diff --git a/.circleci/config.yml b/.circleci/config.yml
index b8d9bb466c21..f29c69235e68 100644
--- a/.circleci/config.yml
+++ b/.circleci/config.yml
@@ -15,7 +15,7 @@ variables:
 jobs:
   get_code:
     docker:
-      - image: cimg/python:3.7
+      - image: cimg/python:3.8
     <<: *set_workdir
     steps:
       # Replace standard code checkout with shallow clone to speed things up.
@@ -73,7 +73,7 @@ jobs:
             - ~/repo
   validate_test_tools:
     docker:
-      - image: cimg/python:3.7
+      - image: cimg/python:3.8
     <<: *set_workdir
     steps:
       - *restore_repo_cache
diff --git a/.github/workflows/api.yaml b/.github/workflows/api.yaml
index 90038e328865..753f669f148f 100644
--- a/.github/workflows/api.yaml
+++ b/.github/workflows/api.yaml
@@ -28,7 +28,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
         chunk: [0, 1]
     services:
       postgres:
diff --git a/.github/workflows/check_test_class_names.yaml b/.github/workflows/check_test_class_names.yaml
index 92db607c7bdb..217995df93e4 100644
--- a/.github/workflows/check_test_class_names.yaml
+++ b/.github/workflows/check_test_class_names.yaml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     steps:
       - uses: actions/checkout@v3
       - uses: actions/setup-python@v4
diff --git a/.github/workflows/converter_tests.yaml b/.github/workflows/converter_tests.yaml
index 359edf8b0d9e..1d8c0ee963b8 100644
--- a/.github/workflows/converter_tests.yaml
+++ b/.github/workflows/converter_tests.yaml
@@ -22,7 +22,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     steps:
       - if: github.event_name == 'schedule'
         run: |
diff --git a/.github/workflows/cwl_conformance.yaml b/.github/workflows/cwl_conformance.yaml
index 716a8ca2bbbd..206bd33798a1 100644
--- a/.github/workflows/cwl_conformance.yaml
+++ b/.github/workflows/cwl_conformance.yaml
@@ -22,7 +22,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
         marker: ['green', 'red and required', 'red and not required']
         conformance-version: ['cwl_conformance_v1_0'] #, 'cwl_conformance_v1_1', 'cwl_conformance_v1_2']
     services:
diff --git a/.github/workflows/db_indexes.yaml b/.github/workflows/db_indexes.yaml
index af8455edd887..88f1d02297af 100644
--- a/.github/workflows/db_indexes.yaml
+++ b/.github/workflows/db_indexes.yaml
@@ -24,11 +24,11 @@ jobs:
       matrix:
         db: ['postgresql', 'sqlite']
         postgresql-version: ['13']
-        python-version: ['3.7']
+        python-version: ['3.8']
         include:
           - db: postgresql
             postgresql-version: '9.6'
-            python-version: '3.7'
+            python-version: '3.8'
     services:
       postgres:
         image: postgres:${{ matrix.postgresql-version }}
diff --git a/.github/workflows/dependencies.yaml b/.github/workflows/dependencies.yaml
index 0d02ae2f1234..31405cc13498 100644
--- a/.github/workflows/dependencies.yaml
+++ b/.github/workflows/dependencies.yaml
@@ -9,17 +9,12 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     steps:
       - uses: actions/checkout@v3
       - uses: actions/setup-python@v4
         with:
-          # poetry requires Python >=3.8, but lint requirements currently need
-          # to be generated with `pip freeze`` on the oldest Python version
-          # supported by Galaxy.
-          python-version: |
-            ${{ matrix.python-version }}
-            3.8
+          python-version: ${{ matrix.python-version }}
       - name: Update dependencies
         run: | 
           python -m venv .venv
diff --git a/.github/workflows/deployment.yaml b/.github/workflows/deployment.yaml
index 441f35e71673..082e93d9328d 100644
--- a/.github/workflows/deployment.yaml
+++ b/.github/workflows/deployment.yaml
@@ -33,7 +33,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     steps:
       - uses: actions/checkout@v3
         with:
diff --git a/.github/workflows/docs.yaml b/.github/workflows/docs.yaml
index 6de9bf997964..9f67f3322488 100644
--- a/.github/workflows/docs.yaml
+++ b/.github/workflows/docs.yaml
@@ -16,7 +16,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     steps:
       - name: Get target branch name (push)
         if: github.event_name == 'push'
diff --git a/.github/workflows/first_startup.yaml b/.github/workflows/first_startup.yaml
index 34093cae9605..10f506dbbedf 100644
--- a/.github/workflows/first_startup.yaml
+++ b/.github/workflows/first_startup.yaml
@@ -21,7 +21,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7', '3.11']
+        python-version: ['3.8', '3.11']
     defaults:
       run:
         shell: bash -l {0}
diff --git a/.github/workflows/framework.yaml b/.github/workflows/framework.yaml
index 5de51cca7cad..53a287c5d7c0 100644
--- a/.github/workflows/framework.yaml
+++ b/.github/workflows/framework.yaml
@@ -25,7 +25,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     services:
       postgres:
         image: postgres:13
diff --git a/.github/workflows/integration.yaml b/.github/workflows/integration.yaml
index d3660517fb72..58aa81dd63c3 100644
--- a/.github/workflows/integration.yaml
+++ b/.github/workflows/integration.yaml
@@ -29,7 +29,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
         chunk: ['0', '1', '2', '3']
     services:
       postgres:
diff --git a/.github/workflows/integration_selenium.yaml b/.github/workflows/integration_selenium.yaml
index e8cf74fb5639..427a41ec1559 100644
--- a/.github/workflows/integration_selenium.yaml
+++ b/.github/workflows/integration_selenium.yaml
@@ -29,7 +29,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     services:
       postgres:
         image: postgres:13
diff --git a/.github/workflows/lint.yaml b/.github/workflows/lint.yaml
index 590f93cd5e2c..037f343d9ee9 100644
--- a/.github/workflows/lint.yaml
+++ b/.github/workflows/lint.yaml
@@ -22,7 +22,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7', '3.11']
+        python-version: ['3.8', '3.11']
     env:
       LINT_PATH: 'lib/galaxy/dependencies/pinned-lint-requirements.txt'
       TYPE_PATH: 'lib/galaxy/dependencies/pinned-typecheck-requirements.txt'
diff --git a/.github/workflows/lint_openapi_schema.yml b/.github/workflows/lint_openapi_schema.yml
index 32ddedf5cd7d..c327fabe72e8 100644
--- a/.github/workflows/lint_openapi_schema.yml
+++ b/.github/workflows/lint_openapi_schema.yml
@@ -20,7 +20,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7', '3.11']
+        python-version: ['3.8', '3.11']
     steps:
       - uses: actions/checkout@v3
         with:
diff --git a/.github/workflows/mulled.yaml b/.github/workflows/mulled.yaml
index a9e84ef28a5e..1b140eb1f6c3 100644
--- a/.github/workflows/mulled.yaml
+++ b/.github/workflows/mulled.yaml
@@ -20,7 +20,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     steps:
       - uses: actions/checkout@v3
         with:
diff --git a/.github/workflows/osx_startup.yaml b/.github/workflows/osx_startup.yaml
index 0015076712b6..d550c6741678 100644
--- a/.github/workflows/osx_startup.yaml
+++ b/.github/workflows/osx_startup.yaml
@@ -18,7 +18,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7', '3.11']
+        python-version: ['3.8', '3.11']
     defaults:
       run:
         shell: bash -l {0}
diff --git a/.github/workflows/performance.yaml b/.github/workflows/performance.yaml
index 4eda60399585..48287a739f8c 100644
--- a/.github/workflows/performance.yaml
+++ b/.github/workflows/performance.yaml
@@ -24,7 +24,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     services:
       postgres:
         image: postgres:13
diff --git a/.github/workflows/publish_artifacts.yaml b/.github/workflows/publish_artifacts.yaml
index fd4d736f1b34..3703d932c0cc 100644
--- a/.github/workflows/publish_artifacts.yaml
+++ b/.github/workflows/publish_artifacts.yaml
@@ -9,7 +9,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
         matrix:
-            python-version: ['3.7']
+            python-version: ['3.8']
     steps:
       - uses: actions/setup-python@v4
         with:
diff --git a/.github/workflows/reports_startup.yaml b/.github/workflows/reports_startup.yaml
index 805686e3d388..5597489d3726 100644
--- a/.github/workflows/reports_startup.yaml
+++ b/.github/workflows/reports_startup.yaml
@@ -18,7 +18,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7', '3.11']
+        python-version: ['3.8', '3.11']
     defaults:
       run:
         shell: bash -l {0}
diff --git a/.github/workflows/selenium.yaml b/.github/workflows/selenium.yaml
index 11cf1886fc2b..b8235cb7869f 100644
--- a/.github/workflows/selenium.yaml
+++ b/.github/workflows/selenium.yaml
@@ -30,7 +30,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
         chunk: [0, 1, 2]
     services:
       postgres:
diff --git a/.github/workflows/test_galaxy_packages.yaml b/.github/workflows/test_galaxy_packages.yaml
index 089d4f45f19e..f2e5d88b52a7 100644
--- a/.github/workflows/test_galaxy_packages.yaml
+++ b/.github/workflows/test_galaxy_packages.yaml
@@ -18,7 +18,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7', '3.11']
+        python-version: ['3.8', '3.11']
     steps:
       - uses: actions/checkout@v3
         with:
diff --git a/.github/workflows/test_galaxy_packages_for_pulsar.yaml b/.github/workflows/test_galaxy_packages_for_pulsar.yaml
index 7f5f5499bc4c..2a8e39cadbce 100644
--- a/.github/workflows/test_galaxy_packages_for_pulsar.yaml
+++ b/.github/workflows/test_galaxy_packages_for_pulsar.yaml
@@ -20,7 +20,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.7']  # don't upgrade, see https://github.com/galaxyproject/galaxy/pull/16649
     steps:
       - uses: actions/checkout@v3
         with:
diff --git a/.github/workflows/toolshed.yaml b/.github/workflows/toolshed.yaml
index 4e3a2b2cac8c..95db16b404ac 100644
--- a/.github/workflows/toolshed.yaml
+++ b/.github/workflows/toolshed.yaml
@@ -21,7 +21,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7', '3.11']
+        python-version: ['3.8', '3.11']
         shed-api: ['v1', 'v2']
         test-install-client: ['galaxy_api', 'standalone']
     services:
diff --git a/.github/workflows/unit-postgres.yaml b/.github/workflows/unit-postgres.yaml
index 6e695fd64082..f2c57b6228d4 100644
--- a/.github/workflows/unit-postgres.yaml
+++ b/.github/workflows/unit-postgres.yaml
@@ -22,7 +22,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     services:
       postgres:
         image: postgres:13
diff --git a/.github/workflows/unit.yaml b/.github/workflows/unit.yaml
index 8ade16541a1f..9c32eae057d6 100644
--- a/.github/workflows/unit.yaml
+++ b/.github/workflows/unit.yaml
@@ -20,7 +20,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7', '3.11']
+        python-version: ['3.8', '3.11']
     steps:
       - uses: actions/checkout@v3
         with:
diff --git a/Makefile b/Makefile
index c1caf63227a9..570e7b346d83 100644
--- a/Makefile
+++ b/Makefile
@@ -52,6 +52,11 @@ format:  ## Format Python code base
 remove-unused-imports:  ## Remove unused imports in Python code base
 	$(IN_VENV) autoflake --in-place --remove-all-unused-imports --recursive --verbose lib/ test/
 
+pyupgrade:  ## Convert older code patterns to Python3.7/3.8 idiomatic ones
+	ack --type=python -f | grep -v '^lib/galaxy/schema/bco/\|^lib/galaxy/schema/drs/\|^lib/tool_shed_client/schema/trs\|^tools/\|^.venv/\|^.tox/\|^lib/galaxy/files/sources/\|^lib/galaxy/job_metrics/\|^lib/galaxy/objectstore/\|^lib/galaxy/tool_util/\|^lib/galaxy/util/\|^test/functional/tools/cwl_tools/' | xargs pyupgrade --py38-plus
+	ack --type=python -f | grep -v '^lib/galaxy/schema/bco/\|^lib/galaxy/schema/drs/\|^lib/tool_shed_client/schema/trs\|^tools/\|^.venv/\|^.tox/\|^lib/galaxy/files/sources/\|^lib/galaxy/job_metrics/\|^lib/galaxy/objectstore/\|^lib/galaxy/tool_util/\|^lib/galaxy/util/\|^test/functional/tools/cwl_tools/' | xargs auto-walrus
+	ack --type=python -f lib/galaxy/files/sources/ lib/galaxy/job_metrics/ lib/galaxy/objectstore/ lib/galaxy/tool_util/ lib/galaxy/util/ | xargs pyupgrade --py37-plus
+
 docs-slides-ready:
 	test -f plantuml.jar ||  wget http://jaist.dl.sourceforge.net/project/plantuml/plantuml.jar
 	java -jar plantuml.jar -c $(DOC_SOURCE_DIR)/slideshow/architecture/images/plantuml_options.txt -tsvg $(SLIDESHOW_DIR)/architecture/images/ *.plantuml.txt
diff --git a/README.rst b/README.rst
index 34d382dc68bb..17e11d9ff098 100644
--- a/README.rst
+++ b/README.rst
@@ -24,12 +24,12 @@ Community support is available at `Galaxy Help <https://help.galaxyproject.org/>
 Galaxy Quickstart
 =================
 
-Galaxy requires Python 3.7 . To check your Python version, run:
+Galaxy requires Python 3.8 . To check your Python version, run:
 
 .. code:: console
 
     $ python -V
-    Python 3.7.6
+    Python 3.8.18
 
 Start Galaxy:
 
diff --git a/client/src/api/schema/schema.ts b/client/src/api/schema/schema.ts
index 4b6887c7444f..7e4f142a7a05 100644
--- a/client/src/api/schema/schema.ts
+++ b/client/src/api/schema/schema.ts
@@ -2422,8 +2422,8 @@ export interface components {
             /**
              * Type
              * @description The digest method used to create the checksum.
-             * The value (e.g. `sha-256`) SHOULD be listed as `Hash Name String` in the https://www.iana.org/assignments/named-information/named-information.xhtml#hash-alg[IANA Named Information Hash Algorithm Registry]. Other values MAY be used, as long as implementors are aware of the issues discussed in https://tools.ietf.org/html/rfc6920#section-9.4[RFC6920].
-             * GA4GH may provide more explicit guidance for use of non-IANA-registered algorithms in the future. Until then, if implementors do choose such an algorithm (e.g. because it's implemented by their storage provider), they SHOULD use an existing standard `type` value such as `md5`, `etag`, `crc32c`, `trunc512`, or `sha1`.
+             * The value (e.g. `sha-256`) SHOULD be listed as `Hash Name String` in the https://www.iana.org/assignments/named-information/named-information.xhtml#hash-alg[IANA Named Information Hash Algorithm Registry]. Other values MAY be used, as long as implementers are aware of the issues discussed in https://tools.ietf.org/html/rfc6920#section-9.4[RFC6920].
+             * GA4GH may provide more explicit guidance for use of non-IANA-registered algorithms in the future. Until then, if implementers do choose such an algorithm (e.g. because it's implemented by their storage provider), they SHOULD use an existing standard `type` value such as `md5`, `etag`, `crc32c`, `trunc512`, or `sha1`.
              * @example sha-256
              */
             type: string;
diff --git a/doc/parse_gx_xsd.py b/doc/parse_gx_xsd.py
index 450f8e4d15c6..d7f3b631712d 100644
--- a/doc/parse_gx_xsd.py
+++ b/doc/parse_gx_xsd.py
@@ -107,8 +107,7 @@ def _build_tag(tag, hide_attributes):
                     assertions_buffer.write(f"#### ``{element.attrib['name']}``:\n\n{doc}\n\n")
             text = text.replace(line, assertions_buffer.getvalue())
     tag_help.write(text)
-    best_practices = _get_bp_link(annotation_el)
-    if best_practices:
+    if best_practices := _get_bp_link(annotation_el):
         tag_help.write("\n\n### Best Practices\n")
         tag_help.write(
             """
diff --git a/doc/source/admin/python.md b/doc/source/admin/python.md
index 23870548c8e3..015f85e08a10 100644
--- a/doc/source/admin/python.md
+++ b/doc/source/admin/python.md
@@ -1,6 +1,6 @@
 # Supported Python versions
 
-Galaxy's core functionality is currently supported on Python **>=3.7** .
+Galaxy's core functionality is currently supported on Python **>=3.8** .
 
 If Galaxy complains about the version of Python you are using:
 
diff --git a/doc/source/dev/debugging_tests.md b/doc/source/dev/debugging_tests.md
index 231747572be7..6bda2d5c92f8 100644
--- a/doc/source/dev/debugging_tests.md
+++ b/doc/source/dev/debugging_tests.md
@@ -52,7 +52,7 @@ index 6647588dfb..c8d82957a1 100644
 @@ -14,7 +14,7 @@ jobs:
        fail-fast: false
        matrix:
-         python-version: ['3.7']
+         python-version: ['3.8']
 -        subset: ['upload_datatype', 'extended_metadata', 'kubernetes', 'not (upload_datatype or extended_metadata or kubernetes)']
 +        subset: ['not (upload_datatype or extended_metadata or kubernetes)']
      services:
diff --git a/lib/galaxy/actions/library.py b/lib/galaxy/actions/library.py
index b1f3a8bbfae8..32d555700166 100644
--- a/lib/galaxy/actions/library.py
+++ b/lib/galaxy/actions/library.py
@@ -224,8 +224,7 @@ def _get_path_paste_uploaded_datasets(self, trans, params, library_bunch, respon
         return uploaded_datasets, 200, None
 
     def _get_path_files_and_folders(self, params, preserve_dirs):
-        problem_response = self._check_path_paste_params(params)
-        if problem_response:
+        if problem_response := self._check_path_paste_params(params):
             return problem_response
         files_and_folders = []
         for line, path in self._paths_list(params):
diff --git a/lib/galaxy/app.py b/lib/galaxy/app.py
index 1c8d04847c09..2048dc1ea18c 100644
--- a/lib/galaxy/app.py
+++ b/lib/galaxy/app.py
@@ -846,8 +846,7 @@ def to_str(self, **kwd):
 
 class ExecutionTimerFactory:
     def __init__(self, config):
-        statsd_host = getattr(config, "statsd_host", None)
-        if statsd_host:
+        if statsd_host := getattr(config, "statsd_host", None):
             from galaxy.web.statsd_client import GalaxyStatsdClient
 
             self.galaxy_statsd_client: Optional[GalaxyStatsdClient] = GalaxyStatsdClient(
diff --git a/lib/galaxy/app_unittest_utils/tools_support.py b/lib/galaxy/app_unittest_utils/tools_support.py
index 77c759499d75..6c94d5eb59da 100644
--- a/lib/galaxy/app_unittest_utils/tools_support.py
+++ b/lib/galaxy/app_unittest_utils/tools_support.py
@@ -123,7 +123,7 @@ class MockContext:
     def __init__(self, model_objects=None):
         self.expunged_all = False
         self.flushed = False
-        self.model_objects = model_objects or defaultdict(lambda: {})
+        self.model_objects = model_objects or defaultdict(dict)
         self.created_objects = []
         self.current = self
 
diff --git a/lib/galaxy/auth/__init__.py b/lib/galaxy/auth/__init__.py
index 932639e75a3a..388e4ac2beb1 100644
--- a/lib/galaxy/auth/__init__.py
+++ b/lib/galaxy/auth/__init__.py
@@ -123,8 +123,7 @@ def active_authenticators(self, email, username, password):
 
 def _get_allow_register(d):
     s = d.get("allow-register", True)
-    lower_s = str(s).lower()
-    if lower_s == "challenge":
+    if (lower_s := str(s).lower()) == "challenge":
         return lower_s
     else:
         return string_as_bool(s)
diff --git a/lib/galaxy/celery/__init__.py b/lib/galaxy/celery/__init__.py
index 84afb8ee18b2..8459893402f0 100644
--- a/lib/galaxy/celery/__init__.py
+++ b/lib/galaxy/celery/__init__.py
@@ -101,8 +101,7 @@ def get_galaxy_app():
 
 @lru_cache(maxsize=1)
 def build_app():
-    kwargs = get_app_properties()
-    if kwargs:
+    if kwargs := get_app_properties():
         kwargs["check_migrate_databases"] = False
         kwargs["use_display_applications"] = False
         kwargs["use_converters"] = False
diff --git a/lib/galaxy/celery/tasks.py b/lib/galaxy/celery/tasks.py
index b94cc8f832b4..828e692ba0a2 100644
--- a/lib/galaxy/celery/tasks.py
+++ b/lib/galaxy/celery/tasks.py
@@ -68,12 +68,12 @@
 log = get_logger(__name__)
 
 
-@lru_cache()
+@lru_cache
 def setup_data_table_manager(app):
     app._configure_tool_data_tables(from_shed_config=False)
 
 
-@lru_cache()
+@lru_cache
 def cached_create_tool_from_representation(app, raw_tool_source):
     return create_tool_from_representation(
         app=app, raw_tool_source=raw_tool_source, tool_dir="", tool_source_class="XmlToolSource"
diff --git a/lib/galaxy/config/__init__.py b/lib/galaxy/config/__init__.py
index 70f43efd9225..836b69a5f7f0 100644
--- a/lib/galaxy/config/__init__.py
+++ b/lib/galaxy/config/__init__.py
@@ -59,11 +59,10 @@
 if TYPE_CHECKING:
     from galaxy.model import User
 
-try:
-    from importlib.resources import files  # type: ignore[attr-defined]
-except ImportError:
-    # Python < 3.9
-    from importlib_resources import files  # type: ignore[no-redef]
+if sys.version_info >= (3, 9):
+    from importlib.resources import files
+else:
+    from importlib_resources import files
 
 log = logging.getLogger(__name__)
 
@@ -1173,7 +1172,6 @@ def _process_config(self, kwargs: Dict[str, Any]) -> None:
         log_destination = kwargs.get("log_destination")
         log_rotate_size = size_to_bytes(unicodify(kwargs.get("log_rotate_size", 0)))
         log_rotate_count = int(kwargs.get("log_rotate_count", 0))
-        galaxy_daemon_log_destination = os.environ.get("GALAXY_DAEMON_LOG")
         if log_destination == "stdout":
             LOGGING_CONFIG_DEFAULT["handlers"]["console"] = {
                 "class": "logging.StreamHandler",
@@ -1192,7 +1190,7 @@ def _process_config(self, kwargs: Dict[str, Any]) -> None:
                 "maxBytes": log_rotate_size,
                 "backupCount": log_rotate_count,
             }
-        if galaxy_daemon_log_destination:
+        if galaxy_daemon_log_destination := os.environ.get("GALAXY_DAEMON_LOG"):
             LOGGING_CONFIG_DEFAULT["handlers"]["files"] = {
                 "class": "logging.handlers.RotatingFileHandler",
                 "formatter": "stack",
diff --git a/lib/galaxy/config/config_manage.py b/lib/galaxy/config/config_manage.py
index 5f91bc0d386c..4f0da4d9ddf6 100644
--- a/lib/galaxy/config/config_manage.py
+++ b/lib/galaxy/config/config_manage.py
@@ -428,8 +428,7 @@ def _replace_file(args: Namespace, f: StringIO, app_desc: App, from_path: str, t
 def _build_sample_yaml(args: Namespace, app_desc: App) -> None:
     schema = app_desc.schema
     f = StringIO()
-    description = getattr(schema, "description", None)
-    if description:
+    if description := getattr(schema, "description", None):
         description = description.lstrip()
         as_comment = "\n".join(f"# {line}" for line in description.split("\n")) + "\n"
         f.write(as_comment)
@@ -519,8 +518,7 @@ def _warn(message: str) -> None:
 
 def _get_option_desc(option: Dict[str, Any]) -> str:
     desc = option["desc"]
-    parent_dir = option.get("path_resolves_to")
-    if parent_dir:
+    if parent_dir := option.get("path_resolves_to"):
         path_resolves = f"The value of this option will be resolved with respect to <{parent_dir}>."
         return f"{desc}\n{path_resolves}" if desc else path_resolves
     return desc
diff --git a/lib/galaxy/config/templates.py b/lib/galaxy/config/templates.py
index e3d438b846a3..fc4b473ce7cc 100644
--- a/lib/galaxy/config/templates.py
+++ b/lib/galaxy/config/templates.py
@@ -10,14 +10,17 @@
 
 from jinja2 import Environment
 
-from galaxy.util.resources import resource_path
+from galaxy.util.resources import (
+    resource_path,
+    Traversable,
+)
 
 TEMPLATE_SEP = ">>>>>>"  # Used to split templates into doc/body sections
 
 
 def render(template_path: str, context: dict, custom_templates_dir: str) -> str:
     """Read and return templated content as string."""
-    with open(_get_template_path(template_path, custom_templates_dir)) as f:
+    with _get_template_path(template_path, custom_templates_dir).open() as f:
         template_str = _get_template_body(f.read())
     tmpl = Environment().from_string(template_str)
     return tmpl.render(**context)
@@ -28,7 +31,7 @@ def _get_template_body(template: str) -> str:
     return template.split(TEMPLATE_SEP, 1)[-1].split("\n", 1)[1]
 
 
-def _get_template_path(relpath: str, custom_templates_dir: str) -> Path:
+def _get_template_path(relpath: str, custom_templates_dir: str) -> Traversable:
     """Return template file path."""
     default_path = resource_path("galaxy.config", "templates") / relpath
     custom_path = Path(custom_templates_dir) / relpath
diff --git a/lib/galaxy/datatypes/binary.py b/lib/galaxy/datatypes/binary.py
index fa5352918990..a2343074f542 100644
--- a/lib/galaxy/datatypes/binary.py
+++ b/lib/galaxy/datatypes/binary.py
@@ -1491,11 +1491,9 @@ def get_index_value(tmp: Union[h5py.Dataset, h5py.Datatype, h5py.Group]):
                         return tmp["_index"]
                     return None
                 else:
-                    index_var = tmp.attrs.get("index")
-                    if index_var is not None:
+                    if (index_var := tmp.attrs.get("index")) is not None:
                         return tmp[index_var]
-                    index_var = tmp.attrs.get("_index")
-                    if index_var is not None:
+                    if (index_var := tmp.attrs.get("_index")) is not None:
                         return tmp[index_var]
                     return None
 
diff --git a/lib/galaxy/datatypes/dataproviders/base.py b/lib/galaxy/datatypes/dataproviders/base.py
index 22dbb6abcb10..6b4949111f31 100644
--- a/lib/galaxy/datatypes/dataproviders/base.py
+++ b/lib/galaxy/datatypes/dataproviders/base.py
@@ -56,8 +56,7 @@ def __new__(cls, name, base_classes, attributes):
             if base_settings:
                 settings.update(base_settings)
         # get settings defined in this class
-        new_settings = attributes.pop("settings", None)
-        if new_settings:
+        if new_settings := attributes.pop("settings", None):
             settings.update(new_settings)
         attributes["settings"] = settings
         return type.__new__(cls, name, base_classes, attributes)
diff --git a/lib/galaxy/datatypes/dataproviders/line.py b/lib/galaxy/datatypes/dataproviders/line.py
index dcbdfc440123..560be0e9e0c8 100644
--- a/lib/galaxy/datatypes/dataproviders/line.py
+++ b/lib/galaxy/datatypes/dataproviders/line.py
@@ -184,8 +184,7 @@ def __iter__(self):
         parent_gen = super().__iter__()
         yield from parent_gen
 
-        last_block = self.handle_last_block()
-        if last_block is not None:
+        if (last_block := self.handle_last_block()) is not None:
             self.num_data_returned += 1
             yield last_block
 
diff --git a/lib/galaxy/datatypes/display_applications/parameters.py b/lib/galaxy/datatypes/display_applications/parameters.py
index ede98fc73f6e..2462ac4a8875 100644
--- a/lib/galaxy/datatypes/display_applications/parameters.py
+++ b/lib/galaxy/datatypes/display_applications/parameters.py
@@ -118,8 +118,7 @@ def _get_dataset_like_object(self, other_values):
         return data
 
     def get_value(self, other_values, dataset_hash, user_hash, trans):
-        data = self._get_dataset_like_object(other_values)
-        if data:
+        if data := self._get_dataset_like_object(other_values):
             return DisplayDataValueWrapper(data, self, other_values, dataset_hash, user_hash, trans)
         return None
 
@@ -165,8 +164,7 @@ def is_preparing(self, other_values):
         return False
 
     def ready(self, other_values):
-        value = self._get_dataset_like_object(other_values)
-        if value:
+        if value := self._get_dataset_like_object(other_values):
             if value.state == value.states.OK:
                 return True
             elif value.state == value.states.ERROR:
diff --git a/lib/galaxy/datatypes/isa.py b/lib/galaxy/datatypes/isa.py
index 74ebb2ec59bf..68cc2c38752f 100644
--- a/lib/galaxy/datatypes/isa.py
+++ b/lib/galaxy/datatypes/isa.py
@@ -129,8 +129,7 @@ def _get_investigation(self, dataset: HasExtraFilesPath) -> Optional["Investigat
         We will use it to parse and access information from the archive."""
 
         investigation = None
-        main_file = self._get_main_file(dataset)
-        if main_file is not None:
+        if (main_file := self._get_main_file(dataset)) is not None:
             investigation = self._make_investigation_instance(main_file)
 
         return investigation
diff --git a/lib/galaxy/datatypes/registry.py b/lib/galaxy/datatypes/registry.py
index d454810834b0..d6c4ac4fc174 100644
--- a/lib/galaxy/datatypes/registry.py
+++ b/lib/galaxy/datatypes/registry.py
@@ -483,8 +483,7 @@ def load_datatype_sniffers(self, root, override=False, compressed_sniffers=None)
         distributed config) or contained within an installed Tool Shed repository.
         """
         sniffer_elem_classes = [e.attrib["type"] for e in self.sniffer_elems]
-        sniffers = root.find("sniffers")
-        if sniffers is not None:
+        if (sniffers := root.find("sniffers")) is not None:
             for elem in sniffers.findall("sniffer"):
                 # Keep a status of the process steps to enable stopping the process of handling the sniffer if necessary.
                 ok = True
diff --git a/lib/galaxy/datatypes/text.py b/lib/galaxy/datatypes/text.py
index 91ea44cd3b3a..e7670856665b 100644
--- a/lib/galaxy/datatypes/text.py
+++ b/lib/galaxy/datatypes/text.py
@@ -6,6 +6,7 @@
 import logging
 import os
 import re
+import shlex
 import subprocess
 import tempfile
 from typing import (
@@ -38,7 +39,6 @@
 )
 from galaxy.util import (
     nice_size,
-    shlex_join,
     string_as_bool,
     unicodify,
 )
@@ -242,7 +242,7 @@ def _display_data_trusted(
                 ofilename = dataset.get_file_name()
                 log.exception(
                     'Command "%s" failed. Could not convert the Jupyter Notebook to HTML, defaulting to plain text.',
-                    shlex_join(cmd),
+                    shlex.join(cmd),
                 )
             return open(ofilename, mode="rb"), headers
 
diff --git a/lib/galaxy/datatypes/util/maf_utilities.py b/lib/galaxy/datatypes/util/maf_utilities.py
index bbfdf7967f51..52c18931c3c9 100644
--- a/lib/galaxy/datatypes/util/maf_utilities.py
+++ b/lib/galaxy/datatypes/util/maf_utilities.py
@@ -658,9 +658,6 @@ def get_starts_ends_fields_from_gene_bed(line):
     if len(fields) < 12:
         raise Exception(f"Not a proper 12 column BED line ({line}).")
     tx_start = int(fields[1])
-    strand = fields[5]
-    if strand != "-":
-        strand = "+"  # Default strand is +
     cds_start = int(fields[6])
     cds_end = int(fields[7])
 
diff --git a/lib/galaxy/dependencies/dev-requirements.txt b/lib/galaxy/dependencies/dev-requirements.txt
index 52ed8a5e480e..8c4bb177cba5 100644
--- a/lib/galaxy/dependencies/dev-requirements.txt
+++ b/lib/galaxy/dependencies/dev-requirements.txt
@@ -1,176 +1,172 @@
-aiohttp==3.8.6 ; python_version >= "3.7" and python_version < "3.12"
-aiosignal==1.3.1 ; python_version >= "3.7" and python_version < "3.12"
-alabaster==0.7.13 ; python_version >= "3.7" and python_version < "3.12"
-amqp==5.2.0 ; python_version >= "3.7" and python_version < "3.12"
-anyio==3.7.1 ; python_version >= "3.7" and python_version < "3.12"
-ase==3.22.1 ; python_version >= "3.7" and python_version < "3.12"
-async-timeout==4.0.3 ; python_version >= "3.7" and python_version < "3.12"
-asynctest==0.13.0 ; python_version >= "3.7" and python_version < "3.8"
-attrs==23.1.0 ; python_version >= "3.7" and python_version < "3.12"
-axe-selenium-python==2.1.6 ; python_version >= "3.7" and python_version < "3.12"
-babel==2.13.1 ; python_version >= "3.7" and python_version < "3.12"
-billiard==3.6.4.0 ; python_version >= "3.7" and python_version < "3.12"
-black==23.3.0 ; python_version >= "3.7" and python_version < "3.12"
-bleach==6.0.0 ; python_version >= "3.7" and python_version < "3.12"
-build==1.0.3 ; python_version >= "3.7" and python_version < "3.12"
-cachecontrol[filecache]==0.13.1 ; python_version >= "3.7" and python_version < "3.12"
-cached-property==1.5.2 ; python_version >= "3.7" and python_version < "3.8"
-celery==5.2.7 ; python_version >= "3.7" and python_version < "3.12"
-certifi==2023.11.17 ; python_version >= "3.7" and python_version < "3.12"
-cffi==1.15.1 ; python_version >= "3.7" and python_version < "3.12"
-charset-normalizer==3.3.2 ; python_version >= "3.7" and python_version < "3.12"
-click-didyoumean==0.3.0 ; python_version >= "3.7" and python_version < "3.12"
-click-plugins==1.1.1 ; python_version >= "3.7" and python_version < "3.12"
-click-repl==0.3.0 ; python_version >= "3.7" and python_version < "3.12"
-click==8.1.7 ; python_version >= "3.7" and python_version < "3.12"
-codespell==2.2.5 ; python_version >= "3.7" and python_version < "3.12"
-colorama==0.4.6 ; python_version >= "3.7" and python_version < "3.12" and (sys_platform == "win32" or platform_system == "Windows" or os_name == "nt")
-coverage[toml]==7.2.7 ; python_version >= "3.7" and python_version < "3.12"
-cryptography==41.0.5 ; python_version >= "3.7" and python_version < "3.12"
-cwltest==2.3.20230825125225 ; python_version >= "3.7" and python_version < "3.12"
-cycler==0.11.0 ; python_version >= "3.7" and python_version < "3.12"
-darker==1.7.2 ; python_version >= "3.7" and python_version < "3.12"
-defusedxml==0.7.1 ; python_version >= "3.7" and python_version < "3.12"
-deprecated==1.2.14 ; python_version >= "3.7" and python_version < "3.12"
-docutils==0.18.1 ; python_version >= "3.7" and python_version < "3.12"
-exceptiongroup==1.2.0 ; python_version >= "3.7" and python_version < "3.11"
-filelock==3.12.2 ; python_version >= "3.7" and python_version < "3.12"
-fluent-logger==0.10.0 ; python_version >= "3.7" and python_version < "3.12"
-fonttools==4.38.0 ; python_version >= "3.7" and python_version < "3.12"
-frozenlist==1.3.3 ; python_version >= "3.7" and python_version < "3.12"
-future==0.18.3 ; python_version >= "3.7" and python_version < "3.12"
-galaxy-release-util==0.1.5 ; python_version >= "3.7" and python_version < "3.12"
-greenlet==2.0.2 ; python_version >= "3.7" and python_version < "3.12"
-h11==0.14.0 ; python_version >= "3.7" and python_version < "3.12"
-httpcore==0.17.3 ; python_version >= "3.7" and python_version < "3.12"
-httpx==0.24.1 ; python_version >= "3.7" and python_version < "3.12"
-idna==3.5 ; python_version >= "3.7" and python_version < "3.12"
-imagesize==1.4.1 ; python_version >= "3.7" and python_version < "3.12"
-importlib-metadata==4.13.0 ; python_version >= "3.7" and python_version < "3.12"
-importlib-resources==5.12.0 ; python_version >= "3.7" and python_version < "3.12"
-iniconfig==2.0.0 ; python_version >= "3.7" and python_version < "3.12"
-isodate==0.6.1 ; python_version >= "3.7" and python_version < "3.12"
-isort==5.11.5 ; python_version >= "3.7" and python_version < "3.12"
-jaraco-classes==3.2.3 ; python_version >= "3.7" and python_version < "3.12"
-jeepney==0.8.0 ; python_version >= "3.7" and python_version < "3.12" and sys_platform == "linux"
-jinja2==3.1.2 ; python_version >= "3.7" and python_version < "3.12"
-junit-xml==1.9 ; python_version >= "3.7" and python_version < "3.12"
-keyring==24.1.1 ; python_version >= "3.7" and python_version < "3.12"
-kiwisolver==1.4.5 ; python_version >= "3.7" and python_version < "3.12"
-kombu==5.2.4 ; python_version >= "3.7" and python_version < "3.12"
-lxml==4.9.3 ; python_version >= "3.7" and python_version < "3.12"
-markdown-it-py==2.2.0 ; python_version >= "3.7" and python_version < "3.12"
-markdown-it-reporter==0.0.2 ; python_version >= "3.7" and python_version < "3.12"
-markupsafe==2.1.3 ; python_version >= "3.7" and python_version < "3.12"
-matplotlib==3.5.3 ; python_version >= "3.7" and python_version < "3.8"
+aiohttp==3.9.1 ; python_version >= "3.8" and python_version < "3.12"
+aiosignal==1.3.1 ; python_version >= "3.8" and python_version < "3.12"
+alabaster==0.7.13 ; python_version >= "3.8" and python_version < "3.12"
+amqp==5.2.0 ; python_version >= "3.8" and python_version < "3.12"
+anyio==4.1.0 ; python_version >= "3.8" and python_version < "3.12"
+ase==3.22.1 ; python_version >= "3.8" and python_version < "3.12"
+async-timeout==4.0.3 ; python_version >= "3.8" and python_version < "3.11"
+attrs==23.1.0 ; python_version >= "3.8" and python_version < "3.12"
+axe-selenium-python==2.1.6 ; python_version >= "3.8" and python_version < "3.12"
+babel==2.13.1 ; python_version >= "3.8" and python_version < "3.12"
+backports-zoneinfo==0.2.1 ; python_version >= "3.8" and python_version < "3.9"
+backports-zoneinfo[tzdata]==0.2.1 ; python_version >= "3.8" and python_version < "3.9"
+billiard==4.2.0 ; python_version >= "3.8" and python_version < "3.12"
+black==23.11.0 ; python_version >= "3.8" and python_version < "3.12"
+build==1.0.3 ; python_version >= "3.8" and python_version < "3.12"
+cachecontrol[filecache]==0.13.1 ; python_version >= "3.8" and python_version < "3.12"
+celery==5.3.6 ; python_version >= "3.8" and python_version < "3.12"
+certifi==2023.11.17 ; python_version >= "3.8" and python_version < "3.12"
+cffi==1.16.0 ; python_version >= "3.8" and python_version < "3.12"
+charset-normalizer==3.3.2 ; python_version >= "3.8" and python_version < "3.12"
+click-didyoumean==0.3.0 ; python_version >= "3.8" and python_version < "3.12"
+click-plugins==1.1.1 ; python_version >= "3.8" and python_version < "3.12"
+click-repl==0.3.0 ; python_version >= "3.8" and python_version < "3.12"
+click==8.1.7 ; python_version >= "3.8" and python_version < "3.12"
+codespell==2.2.6 ; python_version >= "3.8" and python_version < "3.12"
+colorama==0.4.6 ; python_version >= "3.8" and python_version < "3.12" and (sys_platform == "win32" or platform_system == "Windows" or os_name == "nt")
+contourpy==1.1.1 ; python_version >= "3.8" and python_version < "3.12"
+coverage[toml]==7.3.2 ; python_version >= "3.8" and python_version < "3.12"
+cryptography==41.0.5 ; python_version >= "3.8" and python_version < "3.12"
+cwltest==2.4.20231031143732 ; python_version >= "3.8" and python_version < "3.12"
+cycler==0.12.1 ; python_version >= "3.8" and python_version < "3.12"
+darker==1.7.2 ; python_version >= "3.8" and python_version < "3.12"
+defusedxml==0.7.1 ; python_version >= "3.8" and python_version < "3.12"
+deprecated==1.2.14 ; python_version >= "3.8" and python_version < "3.12"
+docutils==0.18.1 ; python_version >= "3.8" and python_version < "3.12"
+exceptiongroup==1.2.0 ; python_version >= "3.8" and python_version < "3.11"
+filelock==3.13.1 ; python_version >= "3.8" and python_version < "3.12"
+fluent-logger==0.10.0 ; python_version >= "3.8" and python_version < "3.12"
+fonttools==4.45.1 ; python_version >= "3.8" and python_version < "3.12"
+frozenlist==1.4.0 ; python_version >= "3.8" and python_version < "3.12"
+future==0.18.3 ; python_version >= "3.8" and python_version < "3.12"
+galaxy-release-util==0.1.5 ; python_version >= "3.8" and python_version < "3.12"
+greenlet==3.0.1 ; python_version >= "3.8" and python_version < "3.12"
+h11==0.14.0 ; python_version >= "3.8" and python_version < "3.12"
+httpcore==1.0.2 ; python_version >= "3.8" and python_version < "3.12"
+httpx==0.25.2 ; python_version >= "3.8" and python_version < "3.12"
+idna==3.6 ; python_version >= "3.8" and python_version < "3.12"
+imagesize==1.4.1 ; python_version >= "3.8" and python_version < "3.12"
+importlib-metadata==6.8.0 ; python_version >= "3.8" and python_version < "3.12"
+importlib-resources==6.1.1 ; python_version >= "3.8" and python_version < "3.12"
+iniconfig==2.0.0 ; python_version >= "3.8" and python_version < "3.12"
+isodate==0.6.1 ; python_version >= "3.8" and python_version < "3.12"
+isort==5.12.0 ; python_version >= "3.8" and python_version < "3.12"
+jaraco-classes==3.3.0 ; python_version >= "3.8" and python_version < "3.12"
+jeepney==0.8.0 ; python_version >= "3.8" and python_version < "3.12" and sys_platform == "linux"
+jinja2==3.1.2 ; python_version >= "3.8" and python_version < "3.12"
+junit-xml==1.9 ; python_version >= "3.8" and python_version < "3.12"
+keyring==24.3.0 ; python_version >= "3.8" and python_version < "3.12"
+kiwisolver==1.4.5 ; python_version >= "3.8" and python_version < "3.12"
+kombu==5.3.4 ; python_version >= "3.8" and python_version < "3.12"
+lxml==4.9.3 ; python_version >= "3.8" and python_version < "3.12"
+markdown-it-py==3.0.0 ; python_version >= "3.8" and python_version < "3.12"
+markdown-it-reporter==0.0.2 ; python_version >= "3.8" and python_version < "3.12"
+markupsafe==2.1.3 ; python_version >= "3.8" and python_version < "3.12"
 matplotlib==3.7.4 ; python_version >= "3.8" and python_version < "3.9"
 matplotlib==3.8.2 ; python_version >= "3.9" and python_version < "3.12"
-mdit-py-plugins==0.3.5 ; python_version >= "3.7" and python_version < "3.12"
-mdurl==0.1.2 ; python_version >= "3.7" and python_version < "3.12"
-mirakuru==2.5.1 ; python_version >= "3.7" and python_version < "3.12"
-mistune==2.0.5 ; python_version >= "3.7" and python_version < "3.12"
-more-itertools==9.1.0 ; python_version >= "3.7" and python_version < "3.12"
-msgpack==1.0.5 ; python_version >= "3.7" and python_version < "3.12"
-multidict==6.0.4 ; python_version >= "3.7" and python_version < "3.12"
-mypy-extensions==1.0.0 ; python_version >= "3.7" and python_version < "3.12"
-myst-parser==1.0.0 ; python_version >= "3.7" and python_version < "3.12"
-numpy==1.21.6 ; python_version >= "3.7" and python_version < "3.8"
+mdit-py-plugins==0.4.0 ; python_version >= "3.8" and python_version < "3.12"
+mdurl==0.1.2 ; python_version >= "3.8" and python_version < "3.12"
+mirakuru==2.5.2 ; python_version >= "3.8" and python_version < "3.12"
+mistune==2.0.5 ; python_version >= "3.8" and python_version < "3.12"
+more-itertools==10.1.0 ; python_version >= "3.8" and python_version < "3.12"
+msgpack==1.0.7 ; python_version >= "3.8" and python_version < "3.12"
+multidict==6.0.4 ; python_version >= "3.8" and python_version < "3.12"
+mypy-extensions==1.0.0 ; python_version >= "3.8" and python_version < "3.12"
+myst-parser==2.0.0 ; python_version >= "3.8" and python_version < "3.12"
+nh3==0.2.14 ; python_version >= "3.8" and python_version < "3.12"
 numpy==1.24.4 ; python_version >= "3.8" and python_version < "3.9"
 numpy==1.26.2 ; python_version >= "3.9" and python_version < "3.12"
-outcome==1.3.0.post0 ; python_version >= "3.7" and python_version < "3.12"
-packaging==23.2 ; python_version >= "3.7" and python_version < "3.12"
-pathspec==0.11.2 ; python_version >= "3.7" and python_version < "3.12"
-pillow==9.5.0 ; python_version >= "3.7" and python_version < "3.12"
-pkce==1.0.3 ; python_version >= "3.7" and python_version < "3.12"
-pkginfo==1.9.6 ; python_version >= "3.7" and python_version < "3.12"
-platformdirs==4.0.0 ; python_version >= "3.7" and python_version < "3.12"
-playwright==1.35.0 ; python_version >= "3.7" and python_version < "3.12"
-pluggy==1.2.0 ; python_version >= "3.7" and python_version < "3.12"
-port-for==0.7.1 ; python_version >= "3.7" and python_version < "3.12"
-prettytable==3.7.0 ; python_version >= "3.7" and python_version < "3.12"
-prompt-toolkit==3.0.41 ; python_version >= "3.7" and python_version < "3.12"
-psutil==5.9.6 ; python_version >= "3.7" and python_version < "3.12" and sys_platform != "cygwin"
-py==1.11.0 ; python_version >= "3.7" and python_version < "3.12"
-pycparser==2.21 ; python_version >= "3.7" and python_version < "3.12"
-pyee==9.0.4 ; python_version >= "3.7" and python_version < "3.12"
-pygithub==2.1.1 ; python_version >= "3.7" and python_version < "3.12"
-pygments==2.17.2 ; python_version >= "3.7" and python_version < "3.12"
-pyjwt[crypto]==2.8.0 ; python_version >= "3.7" and python_version < "3.12"
-pynacl==1.5.0 ; python_version >= "3.7" and python_version < "3.12"
-pyparsing==3.0.9 ; python_version >= "3.7" and python_version < "3.12"
-pyproject-hooks==1.0.0 ; python_version >= "3.7" and python_version < "3.12"
-pysocks==1.7.1 ; python_version >= "3.7" and python_version < "3.12"
-pytest-asyncio==0.21.1 ; python_version >= "3.7" and python_version < "3.12"
-pytest-base-url==2.0.0 ; python_version >= "3.7" and python_version < "3.12"
-pytest-celery==0.0.0 ; python_version >= "3.7" and python_version < "3.12"
-pytest-cov==4.1.0 ; python_version >= "3.7" and python_version < "3.12"
-pytest-html==3.2.0 ; python_version >= "3.7" and python_version < "3.12"
-pytest-httpserver==1.0.6 ; python_version >= "3.7" and python_version < "3.12"
-pytest-json-report==1.5.0 ; python_version >= "3.7" and python_version < "3.12"
-pytest-metadata==3.0.0 ; python_version >= "3.7" and python_version < "3.12"
-pytest-mock==3.11.1 ; python_version >= "3.7" and python_version < "3.12"
-pytest-playwright==0.3.3 ; python_version >= "3.7" and python_version < "3.12"
-pytest-postgresql==4.1.1 ; python_version >= "3.7" and python_version < "3.12"
-pytest-shard==0.1.2 ; python_version >= "3.7" and python_version < "3.12"
-pytest==7.4.3 ; python_version >= "3.7" and python_version < "3.12"
-python-dateutil==2.8.2 ; python_version >= "3.7" and python_version < "3.12"
-python-irodsclient==1.1.9 ; python_version >= "3.7" and python_version < "3.12"
-python-slugify==8.0.1 ; python_version >= "3.7" and python_version < "3.12"
-pytz==2023.3.post1 ; python_version >= "3.7" and python_version < "3.12"
-pywin32-ctypes==0.2.2 ; python_version >= "3.7" and python_version < "3.12" and sys_platform == "win32"
-pyyaml==6.0.1 ; python_version >= "3.7" and python_version < "3.12"
-rdflib==6.2.0 ; python_version >= "3.7" and python_version < "3.12"
-readme-renderer==37.3 ; python_version >= "3.7" and python_version < "3.12"
-requests-toolbelt==1.0.0 ; python_version >= "3.7" and python_version < "3.12"
-requests==2.31.0 ; python_version >= "3.7" and python_version < "3.12"
-responses==0.23.3 ; python_version >= "3.7" and python_version < "3.12"
-rfc3986==2.0.0 ; python_version >= "3.7" and python_version < "3.12"
-rich==13.7.0 ; python_version >= "3.7" and python_version < "3.12"
-ruamel-yaml-clib==0.2.8 ; platform_python_implementation == "CPython" and python_version < "3.11" and python_version >= "3.7"
-ruamel-yaml==0.17.21 ; python_version >= "3.7" and python_version < "3.12"
-schema-salad==8.4.20230808163024 ; python_version >= "3.7" and python_version < "3.12"
-scipy==1.7.3 ; python_version >= "3.7" and python_version < "3.8"
+outcome==1.3.0.post0 ; python_version >= "3.8" and python_version < "3.12"
+packaging==23.2 ; python_version >= "3.8" and python_version < "3.12"
+pathspec==0.11.2 ; python_version >= "3.8" and python_version < "3.12"
+pillow==10.1.0 ; python_version >= "3.8" and python_version < "3.12"
+pkce==1.0.3 ; python_version >= "3.8" and python_version < "3.12"
+pkginfo==1.9.6 ; python_version >= "3.8" and python_version < "3.12"
+platformdirs==4.0.0 ; python_version >= "3.8" and python_version < "3.12"
+playwright==1.40.0 ; python_version >= "3.8" and python_version < "3.12"
+pluggy==1.3.0 ; python_version >= "3.8" and python_version < "3.12"
+port-for==0.7.2 ; python_version >= "3.8" and python_version < "3.12"
+prettytable==3.9.0 ; python_version >= "3.8" and python_version < "3.12"
+prompt-toolkit==3.0.41 ; python_version >= "3.8" and python_version < "3.12"
+psutil==5.9.6 ; python_version >= "3.8" and python_version < "3.12" and sys_platform != "cygwin"
+psycopg==3.1.13 ; python_version >= "3.8" and python_version < "3.12"
+pycparser==2.21 ; python_version >= "3.8" and python_version < "3.12"
+pyee==11.0.1 ; python_version >= "3.8" and python_version < "3.12"
+pygithub==2.1.1 ; python_version >= "3.8" and python_version < "3.12"
+pygments==2.17.2 ; python_version >= "3.8" and python_version < "3.12"
+pyjwt[crypto]==2.8.0 ; python_version >= "3.8" and python_version < "3.12"
+pynacl==1.5.0 ; python_version >= "3.8" and python_version < "3.12"
+pyparsing==3.1.1 ; python_version >= "3.8" and python_version < "3.12"
+pyproject-hooks==1.0.0 ; python_version >= "3.8" and python_version < "3.12"
+pysocks==1.7.1 ; python_version >= "3.8" and python_version < "3.12"
+pytest-asyncio==0.21.1 ; python_version >= "3.8" and python_version < "3.12"
+pytest-base-url==2.0.0 ; python_version >= "3.8" and python_version < "3.12"
+pytest-celery==0.0.0 ; python_version >= "3.8" and python_version < "3.12"
+pytest-cov==4.1.0 ; python_version >= "3.8" and python_version < "3.12"
+pytest-html==4.1.1 ; python_version >= "3.8" and python_version < "3.12"
+pytest-httpserver==1.0.8 ; python_version >= "3.8" and python_version < "3.12"
+pytest-json-report==1.5.0 ; python_version >= "3.8" and python_version < "3.12"
+pytest-metadata==3.0.0 ; python_version >= "3.8" and python_version < "3.12"
+pytest-mock==3.12.0 ; python_version >= "3.8" and python_version < "3.12"
+pytest-playwright==0.4.3 ; python_version >= "3.8" and python_version < "3.12"
+pytest-postgresql==5.0.0 ; python_version >= "3.8" and python_version < "3.12"
+pytest-shard==0.1.2 ; python_version >= "3.8" and python_version < "3.12"
+pytest==7.4.3 ; python_version >= "3.8" and python_version < "3.12"
+python-dateutil==2.8.2 ; python_version >= "3.8" and python_version < "3.12"
+python-irodsclient==1.1.9 ; python_version >= "3.8" and python_version < "3.12"
+python-slugify==8.0.1 ; python_version >= "3.8" and python_version < "3.12"
+pytz==2023.3.post1 ; python_version >= "3.8" and python_version < "3.9"
+pywin32-ctypes==0.2.2 ; python_version >= "3.8" and python_version < "3.12" and sys_platform == "win32"
+pyyaml==6.0.1 ; python_version >= "3.8" and python_version < "3.12"
+rdflib==6.3.2 ; python_version >= "3.8" and python_version < "3.12"
+readme-renderer==42.0 ; python_version >= "3.8" and python_version < "3.12"
+requests-toolbelt==1.0.0 ; python_version >= "3.8" and python_version < "3.12"
+requests==2.31.0 ; python_version >= "3.8" and python_version < "3.12"
+responses==0.24.1 ; python_version >= "3.8" and python_version < "3.12"
+rfc3986==2.0.0 ; python_version >= "3.8" and python_version < "3.12"
+rich==13.7.0 ; python_version >= "3.8" and python_version < "3.12"
+ruamel-yaml-clib==0.2.8 ; platform_python_implementation == "CPython" and python_version < "3.12" and python_version >= "3.8"
+ruamel-yaml==0.18.5 ; python_version >= "3.8" and python_version < "3.12"
+schema-salad==8.4.20231117150958 ; python_version >= "3.8" and python_version < "3.12"
 scipy==1.10.1 ; python_version >= "3.8" and python_version < "3.9"
 scipy==1.11.4 ; python_version >= "3.9" and python_version < "3.12"
-secretstorage==3.3.3 ; python_version >= "3.7" and python_version < "3.12" and sys_platform == "linux"
-selenium==4.11.2 ; python_version >= "3.7" and python_version < "3.12"
-seletools==1.4.0 ; python_version >= "3.7" and python_version < "3.12"
-setuptools==68.0.0 ; python_version >= "3.7" and python_version < "3.12"
-six==1.16.0 ; python_version >= "3.7" and python_version < "3.12"
-sniffio==1.3.0 ; python_version >= "3.7" and python_version < "3.12"
-snowballstemmer==2.2.0 ; python_version >= "3.7" and python_version < "3.12"
-sortedcontainers==2.4.0 ; python_version >= "3.7" and python_version < "3.12"
-sphinx-rtd-theme==1.3.0 ; python_version >= "3.7" and python_version < "3.12"
-sphinx==5.3.0 ; python_version >= "3.7" and python_version < "3.12"
-sphinxcontrib-applehelp==1.0.2 ; python_version >= "3.7" and python_version < "3.12"
-sphinxcontrib-devhelp==1.0.2 ; python_version >= "3.7" and python_version < "3.12"
-sphinxcontrib-htmlhelp==2.0.0 ; python_version >= "3.7" and python_version < "3.12"
-sphinxcontrib-jquery==4.1 ; python_version >= "3.7" and python_version < "3.12"
-sphinxcontrib-jsmath==1.0.1 ; python_version >= "3.7" and python_version < "3.12"
-sphinxcontrib-qthelp==1.0.3 ; python_version >= "3.7" and python_version < "3.12"
-sphinxcontrib-serializinghtml==1.1.5 ; python_version >= "3.7" and python_version < "3.12"
-statsd==4.0.1 ; python_version >= "3.7" and python_version < "3.12"
-testfixtures==7.2.2 ; python_version >= "3.7" and python_version < "3.12"
-text-unidecode==1.3 ; python_version >= "3.7" and python_version < "3.12"
-tinydb==4.8.0 ; python_version >= "3.7" and python_version < "3.12"
-toml==0.10.2 ; python_version >= "3.7" and python_version < "3.12"
-tomli==2.0.1 ; python_version >= "3.7" and python_full_version <= "3.11.0a6"
-trio-websocket==0.11.1 ; python_version >= "3.7" and python_version < "3.12"
-trio==0.22.2 ; python_version >= "3.7" and python_version < "3.12"
-tuspy==1.0.1 ; python_version >= "3.7" and python_version < "3.12"
-twill==3.1 ; python_version >= "3.7" and python_version < "3.12"
-twine==4.0.2 ; python_version >= "3.7" and python_version < "3.12"
-typed-ast==1.5.5 ; python_version < "3.8" and implementation_name == "cpython" and python_version >= "3.7"
-types-pyyaml==6.0.12.12 ; python_version >= "3.7" and python_version < "3.12"
-typing-extensions==4.7.1 ; python_version >= "3.7" and python_version < "3.12"
-urllib3==1.26.18 ; python_version >= "3.7" and python_version < "3.12"
-urllib3[socks]==1.26.18 ; python_version >= "3.7" and python_version < "3.12"
-vine==5.1.0 ; python_version >= "3.7" and python_version < "3.12"
-watchdog==3.0.0 ; python_version >= "3.7" and python_version < "3.12"
-wcwidth==0.2.12 ; python_version >= "3.7" and python_version < "3.12"
-webencodings==0.5.1 ; python_version >= "3.7" and python_version < "3.12"
-werkzeug==2.2.3 ; python_version >= "3.7" and python_version < "3.12"
-wrapt==1.16.0 ; python_version >= "3.7" and python_version < "3.12"
-wsproto==1.2.0 ; python_version >= "3.7" and python_version < "3.12"
-yarl==1.9.3 ; python_version >= "3.7" and python_version < "3.12"
-zipp==3.15.0 ; python_version >= "3.7" and python_version < "3.12"
+secretstorage==3.3.3 ; python_version >= "3.8" and python_version < "3.12" and sys_platform == "linux"
+selenium==4.15.2 ; python_version >= "3.8" and python_version < "3.12"
+seletools==1.4.0 ; python_version >= "3.8" and python_version < "3.12"
+setuptools==69.0.2 ; python_version >= "3.8" and python_version < "3.12"
+six==1.16.0 ; python_version >= "3.8" and python_version < "3.12"
+sniffio==1.3.0 ; python_version >= "3.8" and python_version < "3.12"
+snowballstemmer==2.2.0 ; python_version >= "3.8" and python_version < "3.12"
+sortedcontainers==2.4.0 ; python_version >= "3.8" and python_version < "3.12"
+sphinx-rtd-theme==1.3.0 ; python_version >= "3.8" and python_version < "3.12"
+sphinx==7.1.2 ; python_version >= "3.8" and python_version < "3.12"
+sphinxcontrib-applehelp==1.0.4 ; python_version >= "3.8" and python_version < "3.12"
+sphinxcontrib-devhelp==1.0.2 ; python_version >= "3.8" and python_version < "3.12"
+sphinxcontrib-htmlhelp==2.0.1 ; python_version >= "3.8" and python_version < "3.12"
+sphinxcontrib-jquery==4.1 ; python_version >= "3.8" and python_version < "3.12"
+sphinxcontrib-jsmath==1.0.1 ; python_version >= "3.8" and python_version < "3.12"
+sphinxcontrib-qthelp==1.0.3 ; python_version >= "3.8" and python_version < "3.12"
+sphinxcontrib-serializinghtml==1.1.5 ; python_version >= "3.8" and python_version < "3.12"
+statsd==4.0.1 ; python_version >= "3.8" and python_version < "3.12"
+testfixtures==7.2.2 ; python_version >= "3.8" and python_version < "3.12"
+text-unidecode==1.3 ; python_version >= "3.8" and python_version < "3.12"
+tinydb==4.8.0 ; python_version >= "3.8" and python_version < "3.12"
+toml==0.10.2 ; python_version >= "3.8" and python_version < "3.12"
+tomli==2.0.1 ; python_version >= "3.8" and python_full_version <= "3.11.0a6"
+trio-websocket==0.11.1 ; python_version >= "3.8" and python_version < "3.12"
+trio==0.23.1 ; python_version >= "3.8" and python_version < "3.12"
+tuspy==1.0.1 ; python_version >= "3.8" and python_version < "3.12"
+twill==3.2.1 ; python_version >= "3.8" and python_version < "3.12"
+twine==4.0.2 ; python_version >= "3.8" and python_version < "3.12"
+typing-extensions==4.8.0 ; python_version >= "3.8" and python_version < "3.12"
+tzdata==2023.3 ; python_version >= "3.8" and python_version < "3.12"
+urllib3==1.26.18 ; python_version >= "3.8" and python_version < "3.12"
+urllib3[socks]==1.26.18 ; python_version >= "3.8" and python_version < "3.12"
+vine==5.1.0 ; python_version >= "3.8" and python_version < "3.12"
+watchdog==3.0.0 ; python_version >= "3.8" and python_version < "3.12"
+wcwidth==0.2.12 ; python_version >= "3.8" and python_version < "3.12"
+werkzeug==3.0.1 ; python_version >= "3.8" and python_version < "3.12"
+wrapt==1.16.0 ; python_version >= "3.8" and python_version < "3.12"
+wsproto==1.2.0 ; python_version >= "3.8" and python_version < "3.12"
+yarl==1.9.3 ; python_version >= "3.8" and python_version < "3.12"
+zipp==3.17.0 ; python_version >= "3.8" and python_version < "3.12"
diff --git a/lib/galaxy/dependencies/pinned-lint-requirements.txt b/lib/galaxy/dependencies/pinned-lint-requirements.txt
index 6958b57997aa..6663da463581 100644
--- a/lib/galaxy/dependencies/pinned-lint-requirements.txt
+++ b/lib/galaxy/dependencies/pinned-lint-requirements.txt
@@ -1,10 +1,7 @@
 attrs==23.1.0
-flake8==5.0.4
-flake8-bugbear==23.3.12
-importlib-metadata==4.2.0
+flake8==6.1.0
+flake8-bugbear==23.9.16
 mccabe==0.7.0
-pycodestyle==2.9.1
-pyflakes==2.5.0
+pycodestyle==2.11.1
+pyflakes==3.1.0
 ruff==0.1.6
-typing_extensions==4.7.1
-zipp==3.15.0
diff --git a/lib/galaxy/dependencies/pinned-requirements.txt b/lib/galaxy/dependencies/pinned-requirements.txt
index d0c127d7b1b4..35c59dfd9e7f 100644
--- a/lib/galaxy/dependencies/pinned-requirements.txt
+++ b/lib/galaxy/dependencies/pinned-requirements.txt
@@ -1,217 +1,216 @@
-a2wsgi==1.7.0 ; python_version >= "3.7" and python_version < "3.12"
-adal==1.2.7 ; python_version >= "3.7" and python_version < "3.12"
-aiobotocore==2.4.2 ; python_version >= "3.7" and python_version < "3.12"
-aiodataloader==0.4.0 ; python_version >= "3.7" and python_version < "3.12"
-aiofiles==23.2.1 ; python_version >= "3.7" and python_version < "3.12"
-aiohttp==3.8.6 ; python_version >= "3.7" and python_version < "3.12"
-aioitertools==0.11.0 ; python_version >= "3.7" and python_version < "3.12"
-aiosignal==1.3.1 ; python_version >= "3.7" and python_version < "3.12"
-alembic==1.12.1 ; python_version >= "3.7" and python_version < "3.12"
-amqp==5.2.0 ; python_version >= "3.7" and python_version < "3.12"
-aniso8601==9.0.1 ; python_version >= "3.7" and python_version < "3.12"
-anyio==3.7.1 ; python_version >= "3.7" and python_version < "3.12"
-apispec==6.3.0 ; python_version >= "3.7" and python_version < "3.12"
-appdirs==1.4.4 ; python_version >= "3.7" and python_version < "3.12"
-arcp==0.2.1 ; python_version >= "3.7" and python_version < "3.12"
-argcomplete==3.1.2 ; python_version >= "3.7" and python_version < "3.12"
-async-timeout==4.0.3 ; python_version >= "3.7" and python_version < "3.12"
-asynctest==0.13.0 ; python_version >= "3.7" and python_version < "3.8"
-attmap==0.13.2 ; python_version >= "3.7" and python_version < "3.12"
-attrs==23.1.0 ; python_version >= "3.7" and python_version < "3.12"
-babel==2.13.1 ; python_version >= "3.7" and python_version < "3.12"
-backports-zoneinfo==0.2.1 ; python_version >= "3.7" and python_version < "3.9"
-bagit-profile==1.3.1 ; python_version >= "3.7" and python_version < "3.12"
-bagit==1.8.1 ; python_version >= "3.7" and python_version < "3.12"
-bcrypt==4.0.1 ; python_version >= "3.7" and python_version < "3.12"
-bdbag==1.7.1 ; python_version >= "3.7" and python_version < "3.12"
-beaker==1.12.1 ; python_version >= "3.7" and python_version < "3.12"
-billiard==3.6.4.0 ; python_version >= "3.7" and python_version < "3.12"
-bioblend==1.2.0 ; python_version >= "3.7" and python_version < "3.12"
-bleach==6.0.0 ; python_version >= "3.7" and python_version < "3.12"
-boltons==23.1.1 ; python_version >= "3.7" and python_version < "3.12"
-boto==2.49.0 ; python_version >= "3.7" and python_version < "3.12"
-botocore==1.27.59 ; python_version >= "3.7" and python_version < "3.12"
-bx-python==0.10.0 ; python_version >= "3.7" and python_version < "3.12"
-cachecontrol[filecache]==0.13.1 ; python_version >= "3.7" and python_version < "3.12"
-cached-property==1.5.2 ; python_version >= "3.7" and python_version < "3.8"
-celery==5.2.7 ; python_version >= "3.7" and python_version < "3.12"
-certifi==2023.11.17 ; python_version >= "3.7" and python_version < "3.12"
-cffi==1.15.1 ; python_version >= "3.7" and python_version < "3.12"
-charset-normalizer==3.3.2 ; python_version >= "3.7" and python_version < "3.12"
-cheetah3==3.2.6.post1 ; python_version >= "3.7" and python_version < "3.12"
-circus==0.18.0 ; python_version >= "3.7" and python_version < "3.12"
-click-didyoumean==0.3.0 ; python_version >= "3.7" and python_version < "3.12"
-click-plugins==1.1.1 ; python_version >= "3.7" and python_version < "3.12"
-click-repl==0.3.0 ; python_version >= "3.7" and python_version < "3.12"
-click==8.1.7 ; python_version >= "3.7" and python_version < "3.12"
-cloudauthz==0.6.0 ; python_version >= "3.7" and python_version < "3.12"
-cloudbridge==3.2.0 ; python_version >= "3.7" and python_version < "3.12"
-colorama==0.4.6 ; python_version >= "3.7" and python_version < "3.12" and platform_system == "Windows"
-coloredlogs==15.0.1 ; python_version >= "3.7" and python_version < "3.12"
-conda-package-streaming==0.9.0 ; python_version >= "3.7" and python_version < "3.12"
-cryptography==41.0.5 ; python_version >= "3.7" and python_version < "3.12"
-cwl-upgrader==1.2.8 ; python_version >= "3.7" and python_version < "3.12"
-cwl-utils==0.28 ; python_version >= "3.7" and python_version < "3.12"
-cwltool==3.1.20221109155812 ; python_version >= "3.7" and python_version < "3.12"
-decorator==5.1.1 ; python_version >= "3.7" and python_version < "3.12"
-defusedxml==0.7.1 ; python_version >= "3.7" and python_version < "3.12"
-deprecation==2.1.0 ; python_version >= "3.7" and python_version < "3.12"
-dictobj==0.4 ; python_version >= "3.7" and python_version < "3.12"
-dnspython==2.3.0 ; python_version >= "3.7" and python_version < "3.12"
-docopt==0.6.2 ; python_version >= "3.7" and python_version < "3.12"
-docutils==0.18.1 ; python_version >= "3.7" and python_version < "3.12"
-dparse==0.6.3 ; python_version >= "3.7" and python_version < "3.12"
-ecdsa==0.18.0 ; python_version >= "3.7" and python_version < "3.12"
-edam-ontology==1.25.2 ; python_version >= "3.7" and python_version < "3.12"
-email-validator==2.0.0.post2 ; python_version >= "3.7" and python_version < "3.12"
-exceptiongroup==1.2.0 ; python_version >= "3.7" and python_version < "3.11"
-fastapi-utils==0.2.1 ; python_version >= "3.7" and python_version < "3.12"
-fastapi==0.98.0 ; python_version >= "3.7" and python_version < "3.12"
-filelock==3.12.2 ; python_version >= "3.7" and python_version < "3.12"
-frozenlist==1.3.3 ; python_version >= "3.7" and python_version < "3.12"
-fs==2.4.16 ; python_version >= "3.7" and python_version < "3.12"
-fsspec==2023.1.0 ; python_version >= "3.7" and python_version < "3.12"
-future==0.18.3 ; python_version >= "3.7" and python_version < "3.12"
-galaxy-sequence-utils==1.1.5 ; python_version >= "3.7" and python_version < "3.12"
-galaxy2cwl==0.1.4 ; python_version >= "3.7" and python_version < "3.12"
-graphene-sqlalchemy==3.0.0b3 ; python_version >= "3.7" and python_version < "3.12"
-graphene==3.3 ; python_version >= "3.7" and python_version < "3.12"
-graphql-core==3.2.3 ; python_version >= "3.7" and python_version < "3.12"
-graphql-relay==3.2.0 ; python_version >= "3.7" and python_version < "3.12"
-gravity==1.0.4 ; python_version >= "3.7" and python_version < "3.12"
-greenlet==2.0.2 ; python_version >= "3.7" and (platform_machine == "aarch64" or platform_machine == "ppc64le" or platform_machine == "x86_64" or platform_machine == "amd64" or platform_machine == "AMD64" or platform_machine == "win32" or platform_machine == "WIN32") and python_version < "3.12"
-gunicorn==21.2.0 ; python_version >= "3.7" and python_version < "3.12"
-gxformat2==0.18.0 ; python_version >= "3.7" and python_version < "3.12"
-h11==0.14.0 ; python_version >= "3.7" and python_version < "3.12"
-h5grove==1.3.0 ; python_version >= "3.7" and python_version < "3.12"
-h5py==3.8.0 ; python_version >= "3.7" and python_version < "3.12"
-humanfriendly==10.0 ; python_version >= "3.7" and python_version < "3.12"
-idna==3.5 ; python_version >= "3.7" and python_version < "3.12"
-importlib-metadata==4.13.0 ; python_version >= "3.7" and python_version < "3.12"
-importlib-resources==5.12.0 ; python_version >= "3.7" and python_version < "3.12"
-isa-rwval==0.10.10 ; python_version >= "3.7" and python_version < "3.12"
-isodate==0.6.1 ; python_version >= "3.7" and python_version < "3.12"
-jinja2==3.1.2 ; python_version >= "3.7" and python_version < "3.12"
-jmespath==1.0.1 ; python_version >= "3.7" and python_version < "3.12"
-jsonref==1.1.0 ; python_version >= "3.7" and python_version < "3.12"
-jsonschema==4.17.3 ; python_version >= "3.7" and python_version < "3.12"
-kombu==5.2.4 ; python_version >= "3.7" and python_version < "3.12"
-lagom==2.6.0 ; python_version >= "3.7" and python_version < "3.12"
-lxml==4.9.3 ; python_version >= "3.7" and python_version < "3.12"
-mako==1.2.4 ; python_version >= "3.7" and python_version < "3.12"
-markdown-it-py==2.2.0 ; python_version >= "3.7" and python_version < "3.12"
-markdown==3.4.4 ; python_version >= "3.7" and python_version < "3.12"
-markupsafe==2.1.3 ; python_version >= "3.7" and python_version < "3.12"
-mdurl==0.1.2 ; python_version >= "3.7" and python_version < "3.12"
-mercurial==6.6 ; python_version >= "3.7" and python_version < "3.12"
-mistune==2.0.5 ; python_version >= "3.7" and python_version < "3.12"
-mrcfile==1.4.3 ; python_version >= "3.7" and python_version < "3.12"
-msal==1.25.0 ; python_version >= "3.7" and python_version < "3.12"
-msgpack==1.0.5 ; python_version >= "3.7" and python_version < "3.12"
-multidict==6.0.4 ; python_version >= "3.7" and python_version < "3.12"
-mypy-extensions==1.0.0 ; python_version >= "3.7" and python_version < "3.12"
-networkx==2.5 ; python_version >= "3.7" and python_version < "3.12"
-nodeenv==1.8.0 ; python_version >= "3.7" and python_version < "3.12"
-numpy==1.21.6 ; python_version >= "3.7" and python_version < "3.8"
+a2wsgi==1.8.0 ; python_version >= "3.8" and python_version < "3.12"
+adal==1.2.7 ; python_version >= "3.8" and python_version < "3.12"
+aiobotocore==2.7.0 ; python_version >= "3.8" and python_version < "3.12"
+aiodataloader==0.4.0 ; python_version >= "3.8" and python_version < "3.12"
+aiofiles==23.2.1 ; python_version >= "3.8" and python_version < "3.12"
+aiohttp==3.9.1 ; python_version >= "3.8" and python_version < "3.12"
+aioitertools==0.11.0 ; python_version >= "3.8" and python_version < "3.12"
+aiosignal==1.3.1 ; python_version >= "3.8" and python_version < "3.12"
+alembic==1.12.1 ; python_version >= "3.8" and python_version < "3.12"
+amqp==5.2.0 ; python_version >= "3.8" and python_version < "3.12"
+aniso8601==9.0.1 ; python_version >= "3.8" and python_version < "3.12"
+anyio==4.1.0 ; python_version >= "3.8" and python_version < "3.12"
+apispec==6.3.0 ; python_version >= "3.8" and python_version < "3.12"
+appdirs==1.4.4 ; python_version >= "3.8" and python_version < "3.12"
+arcp==0.2.1 ; python_version >= "3.8" and python_version < "3.12"
+argcomplete==3.1.6 ; python_version >= "3.8" and python_version < "3.12"
+async-timeout==4.0.3 ; python_version >= "3.8" and python_version < "3.11"
+attmap==0.13.2 ; python_version >= "3.8" and python_version < "3.12"
+attrs==23.1.0 ; python_version >= "3.8" and python_version < "3.12"
+babel==2.13.1 ; python_version >= "3.8" and python_version < "3.12"
+backports-zoneinfo==0.2.1 ; python_version >= "3.8" and python_version < "3.9"
+backports-zoneinfo[tzdata]==0.2.1 ; python_version >= "3.8" and python_version < "3.9"
+bagit-profile==1.3.1 ; python_version >= "3.8" and python_version < "3.12"
+bagit==1.8.1 ; python_version >= "3.8" and python_version < "3.12"
+bcrypt==4.0.1 ; python_version >= "3.8" and python_version < "3.12"
+bdbag==1.7.1 ; python_version >= "3.8" and python_version < "3.12"
+beaker==1.12.1 ; python_version >= "3.8" and python_version < "3.12"
+billiard==4.2.0 ; python_version >= "3.8" and python_version < "3.12"
+bioblend==1.2.0 ; python_version >= "3.8" and python_version < "3.12"
+bleach==6.1.0 ; python_version >= "3.8" and python_version < "3.12"
+boltons==23.1.1 ; python_version >= "3.8" and python_version < "3.12"
+boto==2.49.0 ; python_version >= "3.8" and python_version < "3.12"
+botocore==1.31.64 ; python_version >= "3.8" and python_version < "3.12"
+bx-python==0.10.0 ; python_version >= "3.8" and python_version < "3.12"
+cachecontrol[filecache]==0.13.1 ; python_version >= "3.8" and python_version < "3.12"
+celery==5.3.6 ; python_version >= "3.8" and python_version < "3.12"
+certifi==2023.11.17 ; python_version >= "3.8" and python_version < "3.12"
+cffi==1.16.0 ; python_version >= "3.8" and python_version < "3.12"
+charset-normalizer==3.3.2 ; python_version >= "3.8" and python_version < "3.12"
+cheetah3==3.2.6.post1 ; python_version >= "3.8" and python_version < "3.12"
+circus==0.18.0 ; python_version >= "3.8" and python_version < "3.12"
+click-didyoumean==0.3.0 ; python_version >= "3.8" and python_version < "3.12"
+click-plugins==1.1.1 ; python_version >= "3.8" and python_version < "3.12"
+click-repl==0.3.0 ; python_version >= "3.8" and python_version < "3.12"
+click==8.1.7 ; python_version >= "3.8" and python_version < "3.12"
+cloudauthz==0.6.0 ; python_version >= "3.8" and python_version < "3.12"
+cloudbridge==3.2.0 ; python_version >= "3.8" and python_version < "3.12"
+colorama==0.4.6 ; python_version >= "3.8" and python_version < "3.12" and platform_system == "Windows"
+coloredlogs==15.0.1 ; python_version >= "3.8" and python_version < "3.12"
+conda-package-streaming==0.9.0 ; python_version >= "3.8" and python_version < "3.12"
+cryptography==41.0.5 ; python_version >= "3.8" and python_version < "3.12"
+cwl-upgrader==1.2.10 ; python_version >= "3.8" and python_version < "3.12"
+cwl-utils==0.31 ; python_version >= "3.8" and python_version < "3.12"
+cwltool==3.1.20231114134824 ; python_version >= "3.8" and python_version < "3.12"
+decorator==5.1.1 ; python_version >= "3.8" and python_version < "3.12"
+defusedxml==0.7.1 ; python_version >= "3.8" and python_version < "3.12"
+deprecation==2.1.0 ; python_version >= "3.8" and python_version < "3.12"
+dictobj==0.4 ; python_version >= "3.8" and python_version < "3.12"
+dnspython==2.4.2 ; python_version >= "3.8" and python_version < "3.12"
+docopt==0.6.2 ; python_version >= "3.8" and python_version < "3.12"
+docutils==0.18.1 ; python_version >= "3.8" and python_version < "3.12"
+dparse==0.6.3 ; python_version >= "3.8" and python_version < "3.12"
+ecdsa==0.18.0 ; python_version >= "3.8" and python_version < "3.12"
+edam-ontology==1.25.2 ; python_version >= "3.8" and python_version < "3.12"
+email-validator==2.1.0.post1 ; python_version >= "3.8" and python_version < "3.12"
+exceptiongroup==1.2.0 ; python_version >= "3.8" and python_version < "3.11"
+fastapi-utils==0.2.1 ; python_version >= "3.8" and python_version < "3.12"
+fastapi==0.98.0 ; python_version >= "3.8" and python_version < "3.12"
+filelock==3.13.1 ; python_version >= "3.8" and python_version < "3.12"
+frozenlist==1.4.0 ; python_version >= "3.8" and python_version < "3.12"
+fs==2.4.16 ; python_version >= "3.8" and python_version < "3.12"
+fsspec==2023.10.0 ; python_version >= "3.8" and python_version < "3.12"
+future==0.18.3 ; python_version >= "3.8" and python_version < "3.12"
+galaxy-sequence-utils==1.1.5 ; python_version >= "3.8" and python_version < "3.12"
+galaxy2cwl==0.1.4 ; python_version >= "3.8" and python_version < "3.12"
+graphene-sqlalchemy==3.0.0b3 ; python_version >= "3.8" and python_version < "3.12"
+graphene==3.3 ; python_version >= "3.8" and python_version < "3.12"
+graphql-core==3.2.3 ; python_version >= "3.8" and python_version < "3.12"
+graphql-relay==3.2.0 ; python_version >= "3.8" and python_version < "3.12"
+gravity==1.0.4 ; python_version >= "3.8" and python_version < "3.12"
+greenlet==3.0.1 ; python_version >= "3.8" and (platform_machine == "aarch64" or platform_machine == "ppc64le" or platform_machine == "x86_64" or platform_machine == "amd64" or platform_machine == "AMD64" or platform_machine == "win32" or platform_machine == "WIN32") and python_version < "3.12"
+gunicorn==21.2.0 ; python_version >= "3.8" and python_version < "3.12"
+gxformat2==0.18.0 ; python_version >= "3.8" and python_version < "3.12"
+h11==0.14.0 ; python_version >= "3.8" and python_version < "3.12"
+h5grove==1.3.0 ; python_version >= "3.8" and python_version < "3.12"
+h5py==3.10.0 ; python_version >= "3.8" and python_version < "3.12"
+humanfriendly==10.0 ; python_version >= "3.8" and python_version < "3.12"
+idna==3.6 ; python_version >= "3.8" and python_version < "3.12"
+importlib-metadata==6.8.0 ; python_version >= "3.8" and python_version < "3.12"
+importlib-resources==6.1.1 ; python_version >= "3.8" and python_version < "3.12"
+isa-rwval==0.10.10 ; python_version >= "3.8" and python_version < "3.12"
+isodate==0.6.1 ; python_version >= "3.8" and python_version < "3.12"
+jinja2==3.1.2 ; python_version >= "3.8" and python_version < "3.12"
+jmespath==1.0.1 ; python_version >= "3.8" and python_version < "3.12"
+jsonref==1.1.0 ; python_version >= "3.8" and python_version < "3.12"
+jsonschema-specifications==2023.11.1 ; python_version >= "3.8" and python_version < "3.12"
+jsonschema==4.20.0 ; python_version >= "3.8" and python_version < "3.12"
+kombu==5.3.4 ; python_version >= "3.8" and python_version < "3.12"
+lagom==2.6.0 ; python_version >= "3.8" and python_version < "3.12"
+lxml==4.9.3 ; python_version >= "3.8" and python_version < "3.12"
+mako==1.3.0 ; python_version >= "3.8" and python_version < "3.12"
+markdown-it-py==3.0.0 ; python_version >= "3.8" and python_version < "3.12"
+markdown==3.5.1 ; python_version >= "3.8" and python_version < "3.12"
+markupsafe==2.1.3 ; python_version >= "3.8" and python_version < "3.12"
+mdurl==0.1.2 ; python_version >= "3.8" and python_version < "3.12"
+mercurial==6.6 ; python_version >= "3.8" and python_version < "3.12"
+mistune==2.0.5 ; python_version >= "3.8" and python_version < "3.12"
+mrcfile==1.4.3 ; python_version >= "3.8" and python_version < "3.12"
+msal==1.25.0 ; python_version >= "3.8" and python_version < "3.12"
+msgpack==1.0.7 ; python_version >= "3.8" and python_version < "3.12"
+multidict==6.0.4 ; python_version >= "3.8" and python_version < "3.12"
+mypy-extensions==1.0.0 ; python_version >= "3.8" and python_version < "3.12"
+networkx==2.5 ; python_version >= "3.8" and python_version < "3.12"
+nodeenv==1.8.0 ; python_version >= "3.8" and python_version < "3.12"
 numpy==1.24.4 ; python_version >= "3.8" and python_version < "3.9"
 numpy==1.26.2 ; python_version >= "3.9" and python_version < "3.12"
-oauthlib==3.2.2 ; python_version >= "3.7" and python_version < "3.12"
-orjson==3.9.7 ; python_version >= "3.7" and python_version < "3.12"
-oyaml==1.0 ; python_version >= "3.7" and python_version < "3.12"
-packaging==23.2 ; python_version >= "3.7" and python_version < "3.12"
-paramiko==3.3.1 ; python_version >= "3.7" and python_version < "3.12"
-parsley==1.3 ; python_version >= "3.7" and python_version < "3.12"
-paste==3.7.1 ; python_version >= "3.7" and python_version < "3.12"
-pastedeploy==3.1.0 ; python_version >= "3.7" and python_version < "3.12"
-pebble==5.0.4 ; python_version >= "3.7" and python_version < "3.12"
-pkgutil-resolve-name==1.3.10 ; python_version >= "3.7" and python_version < "3.9"
-promise==2.3 ; python_version >= "3.7" and python_version < "3.12"
-prompt-toolkit==3.0.41 ; python_version >= "3.7" and python_version < "3.12"
-prov==1.5.1 ; python_version >= "3.7" and python_version < "3.12"
-psutil==5.9.6 ; python_version >= "3.7" and python_version < "3.12"
-pulsar-galaxy-lib==0.15.5 ; python_version >= "3.7" and python_version < "3.12"
-pyasn1==0.5.1 ; python_version >= "3.7" and python_version < "3.12"
-pycparser==2.21 ; python_version >= "3.7" and python_version < "3.12"
-pycryptodome==3.19.0 ; python_version >= "3.7" and python_version < "3.12"
-pydantic-tes==0.1.5 ; python_version >= "3.7" and python_version < "3.12"
-pydantic==1.10.13 ; python_version >= "3.7" and python_version < "3.12"
-pydantic[email]==1.10.13 ; python_version >= "3.7" and python_version < "3.12"
-pydot==1.4.2 ; python_version >= "3.7" and python_version < "3.12"
-pyeventsystem==0.1.0 ; python_version >= "3.7" and python_version < "3.12"
-pyfaidx==0.7.2.2 ; python_version >= "3.7" and python_version < "3.12"
-pygments==2.17.2 ; python_version >= "3.7" and python_version < "3.12"
-pyjwt==2.8.0 ; python_version >= "3.7" and python_version < "3.12"
-pyjwt[crypto]==2.8.0 ; python_version >= "3.7" and python_version < "3.12"
-pykwalify==1.8.0 ; python_version >= "3.7" and python_version < "3.12"
-pylibmagic==0.5.0 ; python_version >= "3.7" and python_version < "3.12"
-pynacl==1.5.0 ; python_version >= "3.7" and python_version < "3.12"
-pyparsing==3.0.9 ; python_version >= "3.7" and python_version < "3.12"
+oauthlib==3.2.2 ; python_version >= "3.8" and python_version < "3.12"
+orjson==3.9.10 ; python_version >= "3.8" and python_version < "3.12"
+oyaml==1.0 ; python_version >= "3.8" and python_version < "3.12"
+packaging==23.2 ; python_version >= "3.8" and python_version < "3.12"
+paramiko==3.3.1 ; python_version >= "3.8" and python_version < "3.12"
+parsley==1.3 ; python_version >= "3.8" and python_version < "3.12"
+paste==3.7.1 ; python_version >= "3.8" and python_version < "3.12"
+pastedeploy==3.1.0 ; python_version >= "3.8" and python_version < "3.12"
+pebble==5.0.4 ; python_version >= "3.8" and python_version < "3.12"
+pkgutil-resolve-name==1.3.10 ; python_version >= "3.8" and python_version < "3.9"
+promise==2.3 ; python_version >= "3.8" and python_version < "3.12"
+prompt-toolkit==3.0.41 ; python_version >= "3.8" and python_version < "3.12"
+prov==1.5.1 ; python_version >= "3.8" and python_version < "3.12"
+psutil==5.9.6 ; python_version >= "3.8" and python_version < "3.12"
+pulsar-galaxy-lib==0.15.5 ; python_version >= "3.8" and python_version < "3.12"
+pyasn1==0.5.1 ; python_version >= "3.8" and python_version < "3.12"
+pycparser==2.21 ; python_version >= "3.8" and python_version < "3.12"
+pycryptodome==3.19.0 ; python_version >= "3.8" and python_version < "3.12"
+pydantic-tes==0.1.5 ; python_version >= "3.8" and python_version < "3.12"
+pydantic==1.10.13 ; python_version >= "3.8" and python_version < "3.12"
+pydantic[email]==1.10.13 ; python_version >= "3.8" and python_version < "3.12"
+pydot==1.4.2 ; python_version >= "3.8" and python_version < "3.12"
+pyeventsystem==0.1.0 ; python_version >= "3.8" and python_version < "3.12"
+pyfaidx==0.7.2.2 ; python_version >= "3.8" and python_version < "3.12"
+pygments==2.17.2 ; python_version >= "3.8" and python_version < "3.12"
+pyjwt==2.8.0 ; python_version >= "3.8" and python_version < "3.12"
+pyjwt[crypto]==2.8.0 ; python_version >= "3.8" and python_version < "3.12"
+pykwalify==1.8.0 ; python_version >= "3.8" and python_version < "3.12"
+pylibmagic==0.5.0 ; python_version >= "3.8" and python_version < "3.12"
+pynacl==1.5.0 ; python_version >= "3.8" and python_version < "3.12"
+pyparsing==3.1.1 ; python_version >= "3.8" and python_version < "3.12"
 pyreadline3==3.4.1 ; sys_platform == "win32" and python_version >= "3.8" and python_version < "3.12"
-pyreadline==2.1 ; sys_platform == "win32" and python_version < "3.8" and python_version >= "3.7"
-pyrsistent==0.19.3 ; python_version >= "3.7" and python_version < "3.12"
-pysam==0.22.0 ; python_version >= "3.7" and python_version < "3.12"
-python-dateutil==2.8.2 ; python_version >= "3.7" and python_version < "3.12"
-python-jose==3.3.0 ; python_version >= "3.7" and python_version < "3.12"
-python-magic==0.4.27 ; python_version >= "3.7" and python_version < "3.12"
-python-multipart==0.0.6 ; python_version >= "3.7" and python_version < "3.12"
-python3-openid==3.2.0 ; python_version >= "3.7" and python_version < "3.12"
-pytz==2023.3.post1 ; python_version >= "3.7" and python_version < "3.12"
-pyyaml==6.0.1 ; python_version >= "3.7" and python_version < "3.12"
-pyzmq==25.1.1 ; python_version >= "3.7" and python_version < "3.12"
-rdflib==6.2.0 ; python_version >= "3.7" and python_version < "3.12"
-refgenconf==0.12.2 ; python_version >= "3.7" and python_version < "3.12"
-regex==2023.10.3 ; python_version >= "3.7" and python_version < "3.12"
-repoze-lru==0.7 ; python_version >= "3.7" and python_version < "3.12"
-requests-oauthlib==1.3.1 ; python_version >= "3.7" and python_version < "3.12"
-requests-toolbelt==1.0.0 ; python_version >= "3.7" and python_version < "3.12"
-requests-unixsocket==0.3.0 ; python_version >= "3.7" and python_version < "3.12"
-requests==2.31.0 ; python_version >= "3.7" and python_version < "3.12"
-rich==13.7.0 ; python_version >= "3.7" and python_version < "3.12"
-rocrate==0.9.0 ; python_version >= "3.7" and python_version < "3.12"
-routes==2.5.1 ; python_version >= "3.7" and python_version < "3.12"
-rsa==4.9 ; python_version >= "3.7" and python_version < "3.12"
-ruamel-yaml-clib==0.2.8 ; platform_python_implementation == "CPython" and python_version < "3.11" and python_version >= "3.7"
-ruamel-yaml==0.17.21 ; python_version >= "3.7" and python_version < "3.12"
-s3fs==2023.1.0 ; python_version >= "3.7" and python_version < "3.12"
-schema-salad==8.4.20230808163024 ; python_version >= "3.7" and python_version < "3.12"
-setuptools-scm==5.0.2 ; python_version >= "3.7" and python_version < "3.12"
-setuptools==68.0.0 ; python_version >= "3.7" and python_version < "3.12"
-shellescape==3.8.1 ; python_version >= "3.7" and python_version < "3.12"
-six==1.16.0 ; python_version >= "3.7" and python_version < "3.12"
-sniffio==1.3.0 ; python_version >= "3.7" and python_version < "3.12"
-social-auth-core[openidconnect]==4.0.3 ; python_version >= "3.7" and python_version < "3.12"
-sortedcontainers==2.4.0 ; python_version >= "3.7" and python_version < "3.12"
-sqlalchemy==1.4.50 ; python_version >= "3.7" and python_version < "3.12"
-sqlitedict==2.1.0 ; python_version >= "3.7" and python_version < "3.12"
-sqlparse==0.4.4 ; python_version >= "3.7" and python_version < "3.12"
-starlette-context==0.3.5 ; python_version >= "3.7" and python_version < "3.12"
-starlette-graphene3==0.6.0 ; python_version >= "3.7" and python_version < "3.12"
-starlette==0.27.0 ; python_version >= "3.7" and python_version < "3.12"
-supervisor==4.2.5 ; python_version >= "3.7" and python_version < "3.12"
-svgwrite==1.4.3 ; python_version >= "3.7" and python_version < "3.12"
-tenacity==8.2.3 ; python_version >= "3.7" and python_version < "3.12"
-tifffile==2021.11.2 ; python_version >= "3.7" and python_version < "3.12"
-tinydb==4.8.0 ; python_version >= "3.7" and python_version < "3.12"
-tomli==2.0.1 ; python_version >= "3.7" and python_version < "3.11"
-tornado==6.2 ; python_version >= "3.7" and python_version < "3.12"
-tqdm==4.66.1 ; python_version >= "3.7" and python_version < "3.12"
-tuspy==1.0.1 ; python_version >= "3.7" and python_version < "3.12"
-tuswsgi==0.5.5 ; python_version >= "3.7" and python_version < "3.12"
-typing-extensions==4.7.1 ; python_version >= "3.7" and python_version < "3.12"
-tzdata==2023.3 ; python_version >= "3.7" and python_version < "3.12" and platform_system == "Windows"
-tzlocal==5.1 ; python_version >= "3.7" and python_version < "3.12"
-ubiquerg==0.6.3 ; python_version >= "3.7" and python_version < "3.12"
-urllib3==1.26.18 ; python_version >= "3.7" and python_version < "3.12"
-uvicorn==0.22.0 ; python_version >= "3.7" and python_version < "3.12"
-uvloop==0.18.0 ; python_version >= "3.7" and python_version < "3.12"
-vine==5.1.0 ; python_version >= "3.7" and python_version < "3.12"
-wcwidth==0.2.12 ; python_version >= "3.7" and python_version < "3.12"
-webencodings==0.5.1 ; python_version >= "3.7" and python_version < "3.12"
-webob==1.8.7 ; python_version >= "3.7" and python_version < "3.12"
-whoosh==2.7.4 ; python_version >= "3.7" and python_version < "3.12"
-wrapt==1.16.0 ; python_version >= "3.7" and python_version < "3.12"
-yacman==0.9.2 ; python_version >= "3.7" and python_version < "3.12"
-yarl==1.9.3 ; python_version >= "3.7" and python_version < "3.12"
-zipp==3.15.0 ; python_version >= "3.7" and python_version < "3.12"
-zipstream-new==1.1.8 ; python_version >= "3.7" and python_version < "3.12"
-zstandard==0.21.0 ; python_version >= "3.7" and python_version < "3.12"
+pysam==0.22.0 ; python_version >= "3.8" and python_version < "3.12"
+python-dateutil==2.8.2 ; python_version >= "3.8" and python_version < "3.12"
+python-jose==3.3.0 ; python_version >= "3.8" and python_version < "3.12"
+python-magic==0.4.27 ; python_version >= "3.8" and python_version < "3.12"
+python-multipart==0.0.6 ; python_version >= "3.8" and python_version < "3.12"
+python3-openid==3.2.0 ; python_version >= "3.8" and python_version < "3.12"
+pytz==2023.3.post1 ; python_version >= "3.8" and python_version < "3.12"
+pyyaml==6.0.1 ; python_version >= "3.8" and python_version < "3.12"
+pyzmq==25.1.1 ; python_version >= "3.8" and python_version < "3.12"
+rdflib==6.3.2 ; python_version >= "3.8" and python_version < "3.12"
+referencing==0.31.0 ; python_version >= "3.8" and python_version < "3.12"
+refgenconf==0.12.2 ; python_version >= "3.8" and python_version < "3.12"
+regex==2023.10.3 ; python_version >= "3.8" and python_version < "3.12"
+repoze-lru==0.7 ; python_version >= "3.8" and python_version < "3.12"
+requests-oauthlib==1.3.1 ; python_version >= "3.8" and python_version < "3.12"
+requests-toolbelt==1.0.0 ; python_version >= "3.8" and python_version < "3.12"
+requests-unixsocket==0.3.0 ; python_version >= "3.8" and python_version < "3.12"
+requests==2.31.0 ; python_version >= "3.8" and python_version < "3.12"
+rich==13.7.0 ; python_version >= "3.8" and python_version < "3.12"
+rocrate==0.9.0 ; python_version >= "3.8" and python_version < "3.12"
+routes==2.5.1 ; python_version >= "3.8" and python_version < "3.12"
+rpds-py==0.13.1 ; python_version >= "3.8" and python_version < "3.12"
+rsa==4.9 ; python_version >= "3.8" and python_version < "3.12"
+ruamel-yaml-clib==0.2.8 ; platform_python_implementation == "CPython" and python_version < "3.12" and python_version >= "3.8"
+ruamel-yaml==0.18.5 ; python_version >= "3.8" and python_version < "3.12"
+s3fs==2023.10.0 ; python_version >= "3.8" and python_version < "3.12"
+schema-salad==8.4.20231117150958 ; python_version >= "3.8" and python_version < "3.12"
+setuptools-scm==5.0.2 ; python_version >= "3.8" and python_version < "3.12"
+setuptools==69.0.2 ; python_version >= "3.8" and python_version < "3.12"
+shellescape==3.8.1 ; python_version >= "3.8" and python_version < "3.12"
+six==1.16.0 ; python_version >= "3.8" and python_version < "3.12"
+sniffio==1.3.0 ; python_version >= "3.8" and python_version < "3.12"
+social-auth-core[openidconnect]==4.0.3 ; python_version >= "3.8" and python_version < "3.12"
+sortedcontainers==2.4.0 ; python_version >= "3.8" and python_version < "3.12"
+sqlalchemy==1.4.50 ; python_version >= "3.8" and python_version < "3.12"
+sqlitedict==2.1.0 ; python_version >= "3.8" and python_version < "3.12"
+sqlparse==0.4.4 ; python_version >= "3.8" and python_version < "3.12"
+starlette-context==0.3.6 ; python_version >= "3.8" and python_version < "3.12"
+starlette-graphene3==0.6.0 ; python_version >= "3.8" and python_version < "3.12"
+starlette==0.27.0 ; python_version >= "3.8" and python_version < "3.12"
+supervisor==4.2.5 ; python_version >= "3.8" and python_version < "3.12"
+svgwrite==1.4.3 ; python_version >= "3.8" and python_version < "3.12"
+tenacity==8.2.3 ; python_version >= "3.8" and python_version < "3.12"
+tifffile==2023.7.10 ; python_version >= "3.8" and python_version < "3.12"
+tinydb==4.8.0 ; python_version >= "3.8" and python_version < "3.12"
+tomli==2.0.1 ; python_version >= "3.8" and python_version < "3.11"
+tornado==6.3.3 ; python_version >= "3.8" and python_version < "3.12"
+tqdm==4.66.1 ; python_version >= "3.8" and python_version < "3.12"
+tuspy==1.0.1 ; python_version >= "3.8" and python_version < "3.12"
+tuswsgi==0.5.5 ; python_version >= "3.8" and python_version < "3.12"
+typing-extensions==4.8.0 ; python_version >= "3.8" and python_version < "3.12"
+tzdata==2023.3 ; python_version >= "3.8" and python_version < "3.12"
+tzlocal==5.2 ; python_version >= "3.8" and python_version < "3.12"
+ubiquerg==0.6.3 ; python_version >= "3.8" and python_version < "3.12"
+urllib3==1.26.18 ; python_version >= "3.8" and python_version < "3.12"
+uvicorn==0.24.0.post1 ; python_version >= "3.8" and python_version < "3.12"
+uvloop==0.19.0 ; python_version >= "3.8" and python_version < "3.12"
+vine==5.1.0 ; python_version >= "3.8" and python_version < "3.12"
+wcwidth==0.2.12 ; python_version >= "3.8" and python_version < "3.12"
+webencodings==0.5.1 ; python_version >= "3.8" and python_version < "3.12"
+webob==1.8.7 ; python_version >= "3.8" and python_version < "3.12"
+whoosh==2.7.4 ; python_version >= "3.8" and python_version < "3.12"
+wrapt==1.16.0 ; python_version >= "3.8" and python_version < "3.12"
+yacman==0.9.2 ; python_version >= "3.8" and python_version < "3.12"
+yarl==1.9.3 ; python_version >= "3.8" and python_version < "3.12"
+zipp==3.17.0 ; python_version >= "3.8" and python_version < "3.12"
+zipstream-new==1.1.8 ; python_version >= "3.8" and python_version < "3.12"
+zstandard==0.22.0 ; python_version >= "3.8" and python_version < "3.12"
diff --git a/lib/galaxy/dependencies/pinned-typecheck-requirements.txt b/lib/galaxy/dependencies/pinned-typecheck-requirements.txt
index a898a0a2c2a1..f25703f5524c 100644
--- a/lib/galaxy/dependencies/pinned-typecheck-requirements.txt
+++ b/lib/galaxy/dependencies/pinned-typecheck-requirements.txt
@@ -1,22 +1,21 @@
-cffi==1.15.1 ; python_version >= "3.7" and python_version < "3.12"
-cryptography==41.0.5 ; python_version >= "3.7" and python_version < "3.12"
-mypy-extensions==1.0.0 ; python_version >= "3.7" and python_version < "3.12"
-mypy==1.4.1 ; python_version >= "3.7" and python_version < "3.12"
-pycparser==2.21 ; python_version >= "3.7" and python_version < "3.12"
-pydantic==1.10.13 ; python_version >= "3.7" and python_version < "3.12"
-tomli==2.0.1 ; python_version >= "3.7" and python_version < "3.11"
-typed-ast==1.5.5 ; python_version >= "3.7" and python_version < "3.8"
-types-bleach==6.1.0.1 ; python_version >= "3.7" and python_version < "3.12"
-types-boto==2.49.18.9 ; python_version >= "3.7" and python_version < "3.12"
-types-contextvars==2.4.7.3 ; python_version >= "3.7" and python_version < "3.12"
-types-dataclasses==0.6.6 ; python_version >= "3.7" and python_version < "3.12"
-types-docutils==0.20.0.3 ; python_version >= "3.7" and python_version < "3.12"
-types-markdown==3.5.0.3 ; python_version >= "3.7" and python_version < "3.12"
-types-paramiko==3.3.0.2 ; python_version >= "3.7" and python_version < "3.12"
-types-pkg-resources==0.1.3 ; python_version >= "3.7" and python_version < "3.12"
-types-python-dateutil==2.8.19.14 ; python_version >= "3.7" and python_version < "3.12"
-types-pyyaml==6.0.12.12 ; python_version >= "3.7" and python_version < "3.12"
-types-requests==2.31.0.6 ; python_version >= "3.7" and python_version < "3.12"
-types-six==1.16.21.9 ; python_version >= "3.7" and python_version < "3.12"
-types-urllib3==1.26.25.14 ; python_version >= "3.7" and python_version < "3.12"
-typing-extensions==4.7.1 ; python_version >= "3.7" and python_version < "3.12"
+cffi==1.16.0 ; python_version >= "3.8" and python_version < "3.12"
+cryptography==41.0.5 ; python_version >= "3.8" and python_version < "3.12"
+mypy-extensions==1.0.0 ; python_version >= "3.8" and python_version < "3.12"
+mypy==1.7.1 ; python_version >= "3.8" and python_version < "3.12"
+pycparser==2.21 ; python_version >= "3.8" and python_version < "3.12"
+pydantic==1.10.13 ; python_version >= "3.8" and python_version < "3.12"
+tomli==2.0.1 ; python_version >= "3.8" and python_version < "3.11"
+types-bleach==6.1.0.1 ; python_version >= "3.8" and python_version < "3.12"
+types-boto==2.49.18.9 ; python_version >= "3.8" and python_version < "3.12"
+types-contextvars==2.4.7.3 ; python_version >= "3.8" and python_version < "3.12"
+types-dataclasses==0.6.6 ; python_version >= "3.8" and python_version < "3.12"
+types-docutils==0.20.0.3 ; python_version >= "3.8" and python_version < "3.12"
+types-markdown==3.5.0.3 ; python_version >= "3.8" and python_version < "3.12"
+types-paramiko==3.3.0.2 ; python_version >= "3.8" and python_version < "3.12"
+types-pkg-resources==0.1.3 ; python_version >= "3.8" and python_version < "3.12"
+types-python-dateutil==2.8.19.14 ; python_version >= "3.8" and python_version < "3.12"
+types-pyyaml==6.0.12.12 ; python_version >= "3.8" and python_version < "3.12"
+types-requests==2.31.0.6 ; python_version >= "3.8" and python_version < "3.12"
+types-six==1.16.21.9 ; python_version >= "3.8" and python_version < "3.12"
+types-urllib3==1.26.25.14 ; python_version >= "3.8" and python_version < "3.12"
+typing-extensions==4.8.0 ; python_version >= "3.8" and python_version < "3.12"
diff --git a/lib/galaxy/dependencies/update.sh b/lib/galaxy/dependencies/update.sh
index 7771ea30791e..01708a54cfe4 100755
--- a/lib/galaxy/dependencies/update.sh
+++ b/lib/galaxy/dependencies/update.sh
@@ -6,7 +6,7 @@
 
 set -e
 
-SUPPORTED_PYTHON_VERSIONS="3.7 3.8 3.9 3.10 3.11"
+SUPPORTED_PYTHON_VERSIONS="3.8 3.9 3.10 3.11"
 NOT_SUPPORTED_NEXT_PYTHON_VERSION="3.12"
 
 this_directory="$(cd "$(dirname "$0")" > /dev/null && pwd)"
diff --git a/lib/galaxy/dependencies/update_lint_requirements.sh b/lib/galaxy/dependencies/update_lint_requirements.sh
index 6f06089aabf8..0225d931d11d 100755
--- a/lib/galaxy/dependencies/update_lint_requirements.sh
+++ b/lib/galaxy/dependencies/update_lint_requirements.sh
@@ -11,7 +11,7 @@ THIS_DIRECTORY="$(cd "$(dirname "$0")" > /dev/null && pwd)"
 
 update_pinned_reqs() {
     VENV=$(mktemp -d "${TMPDIR:-/tmp}/$1_venv.XXXXXXXXXX")
-    python3.7 -m venv "${VENV}"
+    python3.8 -m venv "${VENV}"
     . "${VENV}/bin/activate"
     pip install --upgrade pip setuptools
     pip install -r "${THIS_DIRECTORY}/$1-requirements.txt"
diff --git a/lib/galaxy/files/sources/drs.py b/lib/galaxy/files/sources/drs.py
index a2b8ec6869b1..3e05ca4bd56e 100644
--- a/lib/galaxy/files/sources/drs.py
+++ b/lib/galaxy/files/sources/drs.py
@@ -64,8 +64,7 @@ def _write_from(self, target_path, native_path, user_context=None, opts: Optiona
         raise NotImplementedError()
 
     def score_url_match(self, url: str):
-        match = self._url_regex.match(url)
-        if match:
+        if match := self._url_regex.match(url):
             return match.span()[1]
         else:
             return 0
diff --git a/lib/galaxy/forms/forms.py b/lib/galaxy/forms/forms.py
index 80e6c9067f10..ce8a1c2580fa 100644
--- a/lib/galaxy/forms/forms.py
+++ b/lib/galaxy/forms/forms.py
@@ -54,16 +54,14 @@ def from_elem(self, elem, form_definition_current=None):
         form_type = elem.get("type", None)
         # load layout
         layout = []
-        layouts_elem = elem.find("layout")
-        if layouts_elem:
+        if layouts_elem := elem.find("layout"):
             for layout_elem in layouts_elem.findall("grid"):
                 layout_name = layout_elem.get("name", None)
                 assert layout_name and layout_name not in layout, "Layout grid element requires a unique name."
                 layout.append(layout_name)
         # load fields
         fields = []
-        fields_elem = elem.find("fields")
-        if fields_elem is not None:
+        if (fields_elem := elem.find("fields")) is not None:
             for field_elem in fields_elem.findall("field"):
                 field_type = field_elem.get("type")
                 assert field_type in self.field_type_factories, f"Invalid form field type ( {field_type} )."
diff --git a/lib/galaxy/job_execution/actions/post.py b/lib/galaxy/job_execution/actions/post.py
index 39aa70b8ae52..0ce006d35492 100644
--- a/lib/galaxy/job_execution/actions/post.py
+++ b/lib/galaxy/job_execution/actions/post.py
@@ -140,8 +140,7 @@ def execute_on_mapped_over(
             if step_input and hasattr(step_input, "name"):
                 input_names[input_key] = step_input.name
 
-        new_name = cls._gen_new_name(action, input_names, replacement_dict)
-        if new_name:
+        if new_name := cls._gen_new_name(action, input_names, replacement_dict):
             for name, step_output in step_outputs.items():
                 if action.output_name == "" or name == action.output_name:
                     step_output.name = new_name
@@ -248,8 +247,7 @@ def execute(cls, app, sa_session, action, job, replacement_dict, final_job_state
             if has_collection and hasattr(has_collection, "name"):
                 input_names[input_assoc.name] = has_collection.name
 
-        new_name = cls._gen_new_name(action, input_names, replacement_dict)
-        if new_name:
+        if new_name := cls._gen_new_name(action, input_names, replacement_dict):
             for dataset_assoc in job.output_datasets:
                 if action.output_name == "" or dataset_assoc.name == action.output_name:
                     dataset_assoc.dataset.name = new_name
diff --git a/lib/galaxy/job_execution/output_collect.py b/lib/galaxy/job_execution/output_collect.py
index e251c8655e8c..4b96a03a89c3 100644
--- a/lib/galaxy/job_execution/output_collect.py
+++ b/lib/galaxy/job_execution/output_collect.py
@@ -90,8 +90,7 @@ def permissions(self):
         return self._permissions
 
     def set_default_hda_permissions(self, primary_data):
-        permissions = self.permissions
-        if permissions is not UNSET:
+        if (permissions := self.permissions) is not UNSET:
             self._security_agent.set_all_dataset_permissions(primary_data.dataset, permissions, new=True, flush=False)
 
     def copy_dataset_permissions(self, init_from, primary_data):
@@ -676,9 +675,8 @@ def match(self, dataset_instance, filename, path=None, parent_paths=None):
         pattern = self._pattern_for_dataset(dataset_instance)
         if self.match_relative_path and parent_paths:
             filename = os.path.join(*parent_paths, filename)
-        re_match = re.match(pattern, filename)
         match_object = None
-        if re_match:
+        if re_match := re.match(pattern, filename):
             match_object = RegexCollectedDatasetMatch(re_match, self, filename, path=path)
         return match_object
 
diff --git a/lib/galaxy/job_execution/ports/view.py b/lib/galaxy/job_execution/ports/view.py
index 807c2d5d4b77..86598d0d26b3 100644
--- a/lib/galaxy/job_execution/ports/view.py
+++ b/lib/galaxy/job_execution/ports/view.py
@@ -25,8 +25,7 @@ def register_container_information(self, job_id, **kwd):
 
     # Copy/paste from JobFilesView - TODO: de-duplicate.
     def __authorize_job_access(self, encoded_job_id, **kwargs):
-        key = "job_key"
-        if key not in kwargs:
+        if (key := "job_key") not in kwargs:
             error_message = f"Job files action requires a valid '{key}'."
             raise ObjectAttributeMissingException(error_message)
 
diff --git a/lib/galaxy/jobs/__init__.py b/lib/galaxy/jobs/__init__.py
index 91a0ed792be1..48a22bae5d10 100644
--- a/lib/galaxy/jobs/__init__.py
+++ b/lib/galaxy/jobs/__init__.py
@@ -165,8 +165,7 @@ def job_config_xml_to_dict(config, root):
     config_dict["runners"] = runners
 
     # Parser plugins section populate 'runners' and 'dynamic' in config_dict.
-    plugins = root.find("plugins")
-    if plugins is not None:
+    if (plugins := root.find("plugins")) is not None:
         for plugin in ConfiguresHandlers._findall_with_required(plugins, "plugin", ("id", "type", "load")):
             if plugin.get("type") == "runner":
                 workers = plugin.get("workers", plugins.get("workers", JobConfiguration.DEFAULT_NWORKERS))
@@ -243,8 +242,7 @@ def job_config_xml_to_dict(config, root):
     resource_groups = {}
 
     # Parse resources...
-    resources = root.find("resources")
-    if resources is not None:
+    if (resources := root.find("resources")) is not None:
         default_resource_group = resources.get("default", None)
         if default_resource_group:
             resources_config_dict["default"] = default_resource_group
@@ -259,9 +257,8 @@ def job_config_xml_to_dict(config, root):
     config_dict["resources"] = resources_config_dict
 
     # Parse tool mappings
-    tools = root.find("tools")
     config_dict["tools"] = []
-    if tools is not None:
+    if (tools := root.find("tools")) is not None:
         for tool in tools.findall("tool"):
             # There can be multiple definitions with identical ids, but different params
             tool_mapping_conf = {}
@@ -275,8 +272,7 @@ def job_config_xml_to_dict(config, root):
             config_dict["tools"].append(tool_mapping_conf)
 
     limits_config = []
-    limits = root.find("limits")
-    if limits is not None:
+    if (limits := root.find("limits")) is not None:
         for limit in JobConfiguration._findall_with_required(limits, "limit", ("type",)):
             limit_dict = {}
             for key in ["type", "tag", "id", "window"]:
@@ -1237,8 +1233,7 @@ def prepare(self, compute_environment=None):
 
         def get_special():
             stmt = select(model.JobExportHistoryArchive).filter_by(job=job).limit(1)
-            jeha = self.sa_session.scalars(stmt).first()
-            if jeha:
+            if jeha := self.sa_session.scalars(stmt).first():
                 return jeha.fda
             stmt = select(model.GenomeIndexToolData).filter_by(job=job).limit(1)
             return self.sa_session.scalars(stmt).first()
@@ -1510,8 +1505,7 @@ def is_ready_for_resubmission(self, job=None):
         if job is None:
             job = self.get_job()
 
-        destination_params = job.destination_params
-        if "__resubmit_delay_seconds" in destination_params:
+        if "__resubmit_delay_seconds" in (destination_params := job.destination_params):
             delay = float(destination_params["__resubmit_delay_seconds"])
             if job.seconds_since_updated < delay:
                 return False
@@ -2410,8 +2404,7 @@ def container_monitor_command(self, container, **kwds):
     @property
     def user(self):
         job = self.get_job()
-        user_email = job.get_user_email()
-        if user_email:
+        if user_email := job.get_user_email():
             return user_email
         elif job.galaxy_session is not None:
             return f"anonymous@{job.galaxy_session.remote_addr.split()[-1]}"
diff --git a/lib/galaxy/jobs/command_factory.py b/lib/galaxy/jobs/command_factory.py
index 10b9c137347a..1c2a67da86ad 100644
--- a/lib/galaxy/jobs/command_factory.py
+++ b/lib/galaxy/jobs/command_factory.py
@@ -123,8 +123,7 @@ def build_command(
 
     __handle_remote_command_line_building(commands_builder, job_wrapper, for_pulsar=for_pulsar)
 
-    container_monitor_command = job_wrapper.container_monitor_command(container)
-    if container_monitor_command:
+    if container_monitor_command := job_wrapper.container_monitor_command(container):
         commands_builder.prepend_command(container_monitor_command)
 
     working_directory = remote_job_directory or job_wrapper.working_directory
diff --git a/lib/galaxy/jobs/handler.py b/lib/galaxy/jobs/handler.py
index 2472f874be37..279b853a9198 100644
--- a/lib/galaxy/jobs/handler.py
+++ b/lib/galaxy/jobs/handler.py
@@ -1250,8 +1250,7 @@ def stop(self, job, job_wrapper):
             from galaxy.celery import celery_app
 
             celery_app.control.revoke(job.job_runner_external_id)
-        job_runner_name = job.get_job_runner_name()
-        if job_runner_name is not None:
+        if (job_runner_name := job.get_job_runner_name()) is not None:
             runner_name = job_runner_name.split(":", 1)[0]
             log.debug(f"Stopping job {job_wrapper.get_id_tag()} in {runner_name} runner")
             try:
diff --git a/lib/galaxy/jobs/mapper.py b/lib/galaxy/jobs/mapper.py
index 963a01bb724d..f5bac4e68407 100644
--- a/lib/galaxy/jobs/mapper.py
+++ b/lib/galaxy/jobs/mapper.py
@@ -160,8 +160,7 @@ def __get_expand_function(self, destination):
         rules_module_name = destination.params.get("rules_module")
         rule_modules = self.__get_rule_modules_or_defaults(rules_module_name)
         expand_function = None
-        expand_function_name = destination.params.get("function")
-        if expand_function_name:
+        if expand_function_name := destination.params.get("function"):
             expand_function = self.__last_matching_function_in_modules(rule_modules, expand_function_name)
             if not expand_function:
                 message = ERROR_MESSAGE_RULE_FUNCTION_NOT_FOUND % expand_function_name
diff --git a/lib/galaxy/jobs/runners/__init__.py b/lib/galaxy/jobs/runners/__init__.py
index c14aea726f03..b612103f0475 100644
--- a/lib/galaxy/jobs/runners/__init__.py
+++ b/lib/galaxy/jobs/runners/__init__.py
@@ -217,8 +217,7 @@ def shutdown(self):
         for _ in range(len(self.work_threads)):
             self.work_queue.put((STOP_SIGNAL, None))
 
-        join_timeout = self.app.config.monitor_thread_join_timeout
-        if join_timeout > 0:
+        if (join_timeout := self.app.config.monitor_thread_join_timeout) > 0:
             log.info("Waiting up to %d seconds for job worker threads to shutdown...", join_timeout)
             start = time.time()
             # NOTE: threads that have already joined by now are not going to be logged
diff --git a/lib/galaxy/jobs/runners/aws.py b/lib/galaxy/jobs/runners/aws.py
index bed35ec19c37..fa24add37e45 100644
--- a/lib/galaxy/jobs/runners/aws.py
+++ b/lib/galaxy/jobs/runners/aws.py
@@ -78,8 +78,7 @@ def _add_resource_requirements(destination_params):
         {"type": "VCPU", "value": str(destination_params.get("vcpu"))},
         {"type": "MEMORY", "value": str(destination_params.get("memory"))},
     ]
-    n_gpu = destination_params.get("gpu")
-    if n_gpu:
+    if n_gpu := destination_params.get("gpu"):
         rval.append({"type": "GPU", "value": str(n_gpu)})
     return rval
 
@@ -279,8 +278,7 @@ def _get_mount_volumes(self, destination_params):
         if destination_params.get("platform") == 'Fargate':   # Fargate doesn't support host volumes
             return volumes, mount_points
 
-        ec2_host_volumes = destination_params.get("ec2_host_volumes")
-        if ec2_host_volumes:
+        if (ec2_host_volumes := destination_params.get("ec2_host_volumes")):
             for ix, vol in enumerate(ec2_host_volumes.split(",")):
                 vol = vol.strip()
                 vol_name = "host_vol_" + str(ix)
@@ -356,8 +354,7 @@ def _register_job_definition(self, jd_name, container_image, destination_params)
                 }
             )
         other_kwargs = {}
-        retry_strategy = self._get_retry_strategy(destination_params)
-        if retry_strategy:
+        if (retry_strategy := self._get_retry_strategy(destination_params)):
             other_kwargs["retryStrategy"] = retry_strategy
 
         res = self._batch_client.register_job_definition(
diff --git a/lib/galaxy/jobs/runners/chronos.py b/lib/galaxy/jobs/runners/chronos.py
index 3d1264d8cb17..3bed4dc79a67 100644
--- a/lib/galaxy/jobs/runners/chronos.py
+++ b/lib/galaxy/jobs/runners/chronos.py
@@ -201,9 +201,8 @@ def recover(self, job, job_wrapper):
     @handle_exception_call
     def check_watched_item(self, job_state):
         job_name = job_state.job_id
-        job = self._retrieve_job(job_name)
         # TODO: how can stopped GxIT jobs be handled here?
-        if job:
+        if job := self._retrieve_job(job_name):
             succeeded = job["successCount"]
             errors = job["errorCount"]
             if succeeded > 0:
diff --git a/lib/galaxy/jobs/runners/condor.py b/lib/galaxy/jobs/runners/condor.py
index 6e9862ac9e02..31561b7b219d 100644
--- a/lib/galaxy/jobs/runners/condor.py
+++ b/lib/galaxy/jobs/runners/condor.py
@@ -76,8 +76,7 @@ def queue_job(self, job_wrapper):
                 # HTCondor needs the image as 'docker_image'
                 query_params.update({"docker_image": container.container_id})
 
-        galaxy_slots = query_params.get("request_cpus", None)
-        if galaxy_slots:
+        if galaxy_slots := query_params.get("request_cpus", None):
             galaxy_slots_statement = f'GALAXY_SLOTS="{galaxy_slots}"; export GALAXY_SLOTS; GALAXY_SLOTS_CONFIGURED="1"; export GALAXY_SLOTS_CONFIGURED;'
         else:
             galaxy_slots_statement = 'GALAXY_SLOTS="1"; export GALAXY_SLOTS;'
diff --git a/lib/galaxy/jobs/runners/drmaa.py b/lib/galaxy/jobs/runners/drmaa.py
index 5c1d68f70490..6abb6fbef02e 100644
--- a/lib/galaxy/jobs/runners/drmaa.py
+++ b/lib/galaxy/jobs/runners/drmaa.py
@@ -105,8 +105,7 @@ def url_to_destination(self, url):
         """Convert a legacy URL to a job destination"""
         if not url:
             return
-        native_spec = url.split("/")[2]
-        if native_spec:
+        if native_spec := url.split("/")[2]:
             params = dict(nativeSpecification=native_spec)
             log.debug(f"Converted URL '{url}' to destination runner=drmaa, params={params}")
             return JobDestination(runner="drmaa", params=params)
diff --git a/lib/galaxy/jobs/runners/kubernetes.py b/lib/galaxy/jobs/runners/kubernetes.py
index 90d43aafe504..5709e12bfce9 100644
--- a/lib/galaxy/jobs/runners/kubernetes.py
+++ b/lib/galaxy/jobs/runners/kubernetes.py
@@ -527,8 +527,7 @@ def __get_k8s_containers(self, ajs):
             "volumeMounts": deduplicate_entries(mounts),
         }
 
-        resources = self.__get_resources(ajs.job_wrapper)
-        if resources:
+        if resources := self.__get_resources(ajs.job_wrapper):
             envs = []
             cpu_val = None
             if "requests" in resources:
@@ -585,23 +584,17 @@ def __get_k8s_containers(self, ajs):
         return [k8s_container]
 
     def __get_resources(self, job_wrapper):
-        mem_request = self.__get_memory_request(job_wrapper)
-        cpu_request = self.__get_cpu_request(job_wrapper)
-
-        mem_limit = self.__get_memory_limit(job_wrapper)
-        cpu_limit = self.__get_cpu_limit(job_wrapper)
-
         requests = {}
         limits = {}
 
-        if mem_request:
+        if mem_request := self.__get_memory_request(job_wrapper):
             requests["memory"] = mem_request
-        if cpu_request:
+        if cpu_request := self.__get_cpu_request(job_wrapper):
             requests["cpu"] = cpu_request
 
-        if mem_limit:
+        if mem_limit := self.__get_memory_limit(job_wrapper):
             limits["memory"] = mem_limit
-        if cpu_limit:
+        if cpu_limit := self.__get_cpu_limit(job_wrapper):
             limits["cpu"] = cpu_limit
 
         resources = {}
diff --git a/lib/galaxy/jobs/runners/pbs.py b/lib/galaxy/jobs/runners/pbs.py
index fa22c11b1a70..a1012d1a3705 100644
--- a/lib/galaxy/jobs/runners/pbs.py
+++ b/lib/galaxy/jobs/runners/pbs.py
@@ -132,8 +132,7 @@ def url_to_destination(self, url):
 
         # Determine the queue, set the PBS destination (not the same thing as a Galaxy job destination)
         pbs_destination = f"@{server}"
-        pbs_queue = url_split[3] or None
-        if pbs_queue is not None:
+        if (pbs_queue := url_split[3] or None) is not None:
             pbs_destination = f"{pbs_queue}{pbs_destination}"
 
         params = dict(destination=pbs_destination)
diff --git a/lib/galaxy/jobs/runners/pulsar.py b/lib/galaxy/jobs/runners/pulsar.py
index f9142ee8c59f..003af5a4d3c7 100644
--- a/lib/galaxy/jobs/runners/pulsar.py
+++ b/lib/galaxy/jobs/runners/pulsar.py
@@ -599,8 +599,7 @@ def get_client_from_wrapper(self, job_wrapper):
         if hasattr(job_wrapper, "task_id"):
             job_id = f"{job_id}_{job_wrapper.task_id}"
         params = job_wrapper.job_destination.params.copy()
-        user = job_wrapper.get_job().user
-        if user:
+        if user := job_wrapper.get_job().user:
             for key, value in params.items():
                 if value and isinstance(value, str):
                     params[key] = model.User.expand_user_properties(user, value)
diff --git a/lib/galaxy/jobs/runners/univa.py b/lib/galaxy/jobs/runners/univa.py
index c0d2ecb730c3..3e3455c75e52 100644
--- a/lib/galaxy/jobs/runners/univa.py
+++ b/lib/galaxy/jobs/runners/univa.py
@@ -567,8 +567,7 @@ def _map_qstat_drmaa_states(self, job_id, state, extinfo):
 
 def _parse_time(tstring):
     tme = None
-    m = re.search("([0-9:.]+)", tstring)
-    if m is not None:
+    if (m := re.search("([0-9:.]+)", tstring)) is not None:
         timespl = m.group(1).split(":")
         tme = float(timespl[-1])  # sec
         if len(timespl) > 1:  # min
diff --git a/lib/galaxy/jobs/runners/util/env.py b/lib/galaxy/jobs/runners/util/env.py
index b7a30d895055..3aa62164e17f 100644
--- a/lib/galaxy/jobs/runners/util/env.py
+++ b/lib/galaxy/jobs/runners/util/env.py
@@ -21,11 +21,9 @@ def env_to_statement(env):
     >>> env_to_statement(dict(execute="module load java/1.5.1"))
     'module load java/1.5.1'
     """
-    source_file = env.get("file", None)
-    if source_file:
+    if source_file := env.get("file", None):
         return f". {__escape(source_file, env)}"
-    execute = env.get("execute", None)
-    if execute:
+    if execute := env.get("execute", None):
         return execute
     name = env["name"]
     value = __escape(env["value"], env)
diff --git a/lib/galaxy/jobs/runners/util/sudo.py b/lib/galaxy/jobs/runners/util/sudo.py
index fdc8d74feda4..555625878787 100644
--- a/lib/galaxy/jobs/runners/util/sudo.py
+++ b/lib/galaxy/jobs/runners/util/sudo.py
@@ -16,9 +16,8 @@ def sudo_popen(*args, **kwargs):
     Helper method for building and executing Popen command. This is potentially
     sensetive code so should probably be centralized.
     """
-    user = kwargs.get("user", None)
     full_command = [SUDO_PATH, SUDO_PRESERVE_ENVIRONMENT_ARG]
-    if user:
+    if user := kwargs.get("user", None):
         full_command.extend([SUDO_USER_ARG, user])
     full_command.extend(args)
     log.info(f"About to execute the following sudo command - [{' '.join(full_command)}]")
diff --git a/lib/galaxy/managers/base.py b/lib/galaxy/managers/base.py
index f3abe35e7ceb..216aac5582c3 100644
--- a/lib/galaxy/managers/base.py
+++ b/lib/galaxy/managers/base.py
@@ -1139,8 +1139,7 @@ def _parse_fn_filter(self, attr, op, val):
         if not filter_fn:
             return None
         # parse the val from string using the 'val' parser if present (otherwise, leave as string)
-        val_parser = attr_map.get("val", None)
-        if val_parser:
+        if val_parser := attr_map.get("val", None):
             val = val_parser(val)
 
         # curry/partial and fold the val in there now
@@ -1262,8 +1261,7 @@ def parse_date(self, date_string):
         except ValueError:
             pass
 
-        match = self.date_string_re.match(date_string)
-        if match:
+        if match := self.date_string_re.match(date_string):
             date_string = " ".join(group for group in match.groups() if group)
             return date_string
         raise ValueError("datetime strings must be in the ISO 8601 format and in the UTC")
diff --git a/lib/galaxy/managers/collections.py b/lib/galaxy/managers/collections.py
index 24c695db18b8..d46b74ba7f96 100644
--- a/lib/galaxy/managers/collections.py
+++ b/lib/galaxy/managers/collections.py
@@ -631,9 +631,8 @@ def __load_element(self, trans, element_identifier, hide_source_items, copy_elem
             message = message_template % element_identifier
             raise RequestParameterInvalidException(message)
 
-        tags = element_identifier.pop("tags", None)
         tag_str = ""
-        if tags:
+        if tags := element_identifier.pop("tags", None):
             tag_str = ",".join(str(_) for _ in tags)
         if src_type == "hda":
             hda = self.hda_manager.get_accessible(element_id, trans.user)
diff --git a/lib/galaxy/managers/collections_util.py b/lib/galaxy/managers/collections_util.py
index 60b47c3cdff0..dbf439364d45 100644
--- a/lib/galaxy/managers/collections_util.py
+++ b/lib/galaxy/managers/collections_util.py
@@ -156,8 +156,7 @@ def dictify_element_reference(element, rank_fuzzy_counts=None, recursive=True, s
     are clicked.
     """
     dictified = element.to_dict(view="element")
-    element_object = element.element_object
-    if element_object is not None:
+    if (element_object := element.element_object) is not None:
         object_details = dict(
             id=element_object.id,
             model_class=element_object.__class__.__name__,
diff --git a/lib/galaxy/managers/context.py b/lib/galaxy/managers/context.py
index ec9b5e842998..23caedf81e66 100644
--- a/lib/galaxy/managers/context.py
+++ b/lib/galaxy/managers/context.py
@@ -234,8 +234,7 @@ def anonymous(self) -> bool:
         return self.user is None
 
     def get_current_user_roles(self) -> List[Role]:
-        user = self.user
-        if user:
+        if user := self.user:
             roles = user.all_roles()
         else:
             roles = []
diff --git a/lib/galaxy/managers/groups.py b/lib/galaxy/managers/groups.py
index a0950a4063b8..e8919d4db72a 100644
--- a/lib/galaxy/managers/groups.py
+++ b/lib/galaxy/managers/groups.py
@@ -82,8 +82,7 @@ def update(self, trans: ProvidesAppContext, group_id: int, payload: GroupCreateP
         """
         sa_session = trans.sa_session
         group = self._get_group(sa_session, group_id)
-        name = payload.name
-        if name:
+        if name := payload.name:
             self._check_duplicated_group_name(sa_session, name)
             group.name = name
             sa_session.add(group)
diff --git a/lib/galaxy/managers/history_contents.py b/lib/galaxy/managers/history_contents.py
index fa357af3e3b3..155785e1ca63 100644
--- a/lib/galaxy/managers/history_contents.py
+++ b/lib/galaxy/managers/history_contents.py
@@ -568,8 +568,7 @@ def get_filter(attr, op, val):
                     return sql.column("state").in_(states)
                 raise_filter_err(attr, op, val, "bad op in filter")
 
-        column_filter = get_filter(attr, op, val)
-        if column_filter is not None:
+        if (column_filter := get_filter(attr, op, val)) is not None:
             return self.parsed_filter(filter_type="orm", filter=column_filter)
         return super()._parse_orm_filter(attr, op, val)
 
diff --git a/lib/galaxy/managers/jobs.py b/lib/galaxy/managers/jobs.py
index a1ac6728591a..c8eef282b25b 100644
--- a/lib/galaxy/managers/jobs.py
+++ b/lib/galaxy/managers/jobs.py
@@ -868,8 +868,7 @@ def summarize_destination_params(trans, job):
         "Runner Job ID": job.job_runner_external_id,
         "Handler": job.handler,
     }
-    job_destination_params = job.destination_params
-    if job_destination_params:
+    if job_destination_params := job.destination_params:
         destination_params.update(job_destination_params)
     return destination_params
 
diff --git a/lib/galaxy/managers/markdown_util.py b/lib/galaxy/managers/markdown_util.py
index 5c4226e17bff..982aecc6b4a6 100644
--- a/lib/galaxy/managers/markdown_util.py
+++ b/lib/galaxy/managers/markdown_util.py
@@ -88,9 +88,8 @@ def ready_galaxy_markdown_for_import(trans, external_galaxy_markdown):
     _validate(external_galaxy_markdown, internal=False)
 
     def _remap(container, line):
-        id_match = re.search(ENCODED_ID_PATTERN, line)
         object_id = None
-        if id_match:
+        if id_match := re.search(ENCODED_ID_PATTERN, line):
             object_id = id_match.group(2)
             decoded_id = trans.security.decode_id(object_id)
             line = line.replace(id_match.group(), "%s=%d" % (id_match.group(1), decoded_id))
@@ -232,10 +231,9 @@ def _remap_container(container, line):
         return export_markdown
 
     def _encode_line(self, trans, line):
-        id_match = re.search(UNENCODED_ID_PATTERN, line)
         object_id = None
         encoded_id = None
-        if id_match:
+        if id_match := re.search(UNENCODED_ID_PATTERN, line):
             object_id = int(id_match.group(2))
             encoded_id = trans.security.encode_id(object_id)
             line = line.replace(id_match.group(), f"{id_match.group(1)}={encoded_id}")
@@ -524,9 +522,8 @@ def _display_dataset_content(self, hda, header="Contents"):
     def handle_dataset_as_image(self, line, hda):
         dataset = hda.dataset
         name = hda.name or ""
-        path_match = re.search(PATH_LABEL_PATTERN, line)
 
-        if path_match:
+        if path_match := re.search(PATH_LABEL_PATTERN, line):
             filepath = path_match.group(2)
             file = os.path.join(hda.extra_files_path, filepath)
         else:
@@ -581,9 +578,8 @@ def handle_workflow_display(self, line, stored_workflow, workflow_version: Optio
     def handle_workflow_license(self, line, stored_workflow):
         # workflow_manager = self.trans.app.workflow_manager
         license_manager = LicensesManager()
-        license_id = stored_workflow.latest_workflow.license
         markdown = "*No license specified.*"
-        if license_id:
+        if license_id := stored_workflow.latest_workflow.license:
             try:
                 license_metadata = license_manager.get_license_by_id(license_id)
                 markdown = f"[{license_metadata.name}]({license_metadata.url})"
diff --git a/lib/galaxy/managers/model_stores.py b/lib/galaxy/managers/model_stores.py
index 1e817d130a60..a31e5059668f 100644
--- a/lib/galaxy/managers/model_stores.py
+++ b/lib/galaxy/managers/model_stores.py
@@ -265,8 +265,7 @@ def import_model_store(self, request: ImportModelStoreTaskRequest):
         import_options = ImportOptions(
             allow_library_creation=request.for_library,
         )
-        history_id = request.history_id
-        if history_id:
+        if history_id := request.history_id:
             history = self._sa_session.get(model.History, history_id)
         else:
             history = None
diff --git a/lib/galaxy/managers/notification.py b/lib/galaxy/managers/notification.py
index 0db48f7bfb3e..b0da5b1b9d8e 100644
--- a/lib/galaxy/managers/notification.py
+++ b/lib/galaxy/managers/notification.py
@@ -409,7 +409,7 @@ def resolve_users(self, recipients: NotificationRecipients) -> List[User]:
         user_ids_from_roles_stmt = self._get_all_user_ids_from_roles_query(all_role_ids)
 
         union_stmt = union(user_ids_from_groups_stmt, user_ids_from_roles_stmt)
-        user_ids_from_groups_and_roles = set([id for id, in self.sa_session.execute(union_stmt)])
+        user_ids_from_groups_and_roles = {id for id, in self.sa_session.execute(union_stmt)}
         unique_user_ids.update(user_ids_from_groups_and_roles)
 
         stmt = select(User).where(User.id.in_(unique_user_ids))
@@ -448,7 +448,7 @@ def _expand_group_and_roles_ids(self, group_ids: Set[int], role_ids: Set[int]) -
                 .where(GroupRoleAssociation.role_id.in_(role_ids))
                 .distinct()
             )
-            group_ids_from_roles = set([id for id, in self.sa_session.execute(stmt) if id is not None])
+            group_ids_from_roles = {id for id, in self.sa_session.execute(stmt) if id is not None}
             new_group_ids = group_ids_from_roles - processed_group_ids
 
             # Get role IDs associated with any of the given group IDs
@@ -458,7 +458,7 @@ def _expand_group_and_roles_ids(self, group_ids: Set[int], role_ids: Set[int]) -
                 .where(GroupRoleAssociation.group_id.in_(group_ids))
                 .distinct()
             )
-            role_ids_from_groups = set([id for id, in self.sa_session.execute(stmt) if id is not None])
+            role_ids_from_groups = {id for id, in self.sa_session.execute(stmt) if id is not None}
             new_role_ids = role_ids_from_groups - processed_role_ids
 
             # Stop if there are no new group or role IDs to process
diff --git a/lib/galaxy/managers/pages.py b/lib/galaxy/managers/pages.py
index ded9b71b9b04..72eb50fb6775 100644
--- a/lib/galaxy/managers/pages.py
+++ b/lib/galaxy/managers/pages.py
@@ -263,8 +263,7 @@ def create_page(self, trans, payload: CreatePagePayload):
         page = trans.app.model.Page()
         page.title = payload.title
         page.slug = payload.slug
-        page_annotation = payload.annotation
-        if page_annotation is not None:
+        if (page_annotation := payload.annotation) is not None:
             page_annotation = sanitize_html(page_annotation)
             self.add_item_annotation(trans.sa_session, trans.get_user(), page, page_annotation)
 
diff --git a/lib/galaxy/managers/rbac_secured.py b/lib/galaxy/managers/rbac_secured.py
index 9ead418493fd..a63cd036447b 100644
--- a/lib/galaxy/managers/rbac_secured.py
+++ b/lib/galaxy/managers/rbac_secured.py
@@ -215,8 +215,7 @@ def _user_private_role(self, user):
         return self.user_manager.private_role(user)
 
     def _grant_role(self, dataset, role, flush=True):
-        existing = self.by_role(dataset, role)
-        if existing:
+        if existing := self.by_role(dataset, role):
             return existing
         return self._create(dataset, role, flush=flush)
 
diff --git a/lib/galaxy/managers/workflows.py b/lib/galaxy/managers/workflows.py
index 357ec0deae7c..b6625ac1b356 100644
--- a/lib/galaxy/managers/workflows.py
+++ b/lib/galaxy/managers/workflows.py
@@ -784,9 +784,8 @@ def _workflow_from_raw_description(
         steps_by_external_id: Dict[str, model.WorkflowStep] = {}
 
         # Preload dependent workflows with locally defined content_ids.
-        subworkflows = data.get("subworkflows")
         subworkflow_id_map = None
-        if subworkflows:
+        if subworkflows := data.get("subworkflows"):
             subworkflow_id_map = {}
             for key, subworkflow_dict in subworkflows.items():
                 subworkflow = self.__build_embedded_subworkflow(
@@ -1749,8 +1748,7 @@ def __module_from_dict(
         self.__set_default_label(step, module, step_dict.get("tool_state"))
         module.save_to_step(step, detached=dry_run)
 
-        annotation = step_dict.get("annotation")
-        if annotation:
+        if annotation := step_dict.get("annotation"):
             annotation = sanitize_html(annotation)
             sa_session = None if dry_run else trans.sa_session
             self.add_item_annotation(sa_session, trans.get_user(), step, annotation)
diff --git a/lib/galaxy/metadata/set_metadata.py b/lib/galaxy/metadata/set_metadata.py
index 214fe001ff6d..90549c6ddf6b 100644
--- a/lib/galaxy/metadata/set_metadata.py
+++ b/lib/galaxy/metadata/set_metadata.py
@@ -115,8 +115,7 @@ def set_meta_with_tool_provided(
     # This is intentional due to interplay of overwrite kwd, the fact that some metadata
     # parameters may rely on the values of others, and that we are accepting the
     # values provided by the tool as Truth.
-    extension = dataset_instance.extension
-    if extension == "_sniff_":
+    if (extension := dataset_instance.extension) == "_sniff_":
         try:
             extension = sniff.handle_uploaded_dataset_file(dataset_instance.dataset.get_file_name(), datatypes_registry)
             # We need to both set the extension so it is available to set_meta
diff --git a/lib/galaxy/model/__init__.py b/lib/galaxy/model/__init__.py
index 6c79d51fccb6..0e178f8d98d8 100644
--- a/lib/galaxy/model/__init__.py
+++ b/lib/galaxy/model/__init__.py
@@ -522,8 +522,7 @@ def shrink_and_unicodify(what, stream):
 
     def log_str(self):
         extra = ""
-        safe_id = getattr(self, "id", None)
-        if safe_id is not None:
+        if (safe_id := getattr(self, "id", None)) is not None:
             extra += f"id={safe_id}"
         else:
             extra += "unflushed"
@@ -782,8 +781,7 @@ def __init__(self, email=None, password=None, username=None):
     @property
     def extra_preferences(self):
         data = defaultdict(lambda: None)
-        extra_user_preferences = self.preferences.get("extra_user_preferences")
-        if extra_user_preferences:
+        if extra_user_preferences := self.preferences.get("extra_user_preferences"):
             try:
                 data.update(json.loads(extra_user_preferences))
             except Exception:
@@ -794,8 +792,7 @@ def set_password_cleartext(self, cleartext):
         """
         Set user password to the digest of `cleartext`.
         """
-        message = validate_password_str(cleartext)
-        if message:
+        if message := validate_password_str(cleartext):
             raise Exception(f"Invalid password: {message}")
         if User.use_pbkdf2:
             self.password = galaxy.security.passwords.hash_password(cleartext)
@@ -3819,8 +3816,7 @@ def __init__(self, history, action, role):
 
 class StorableObject:
     def flush(self):
-        sa_session = object_session(self)
-        if sa_session:
+        if sa_session := object_session(self):
             with transaction(sa_session):
                 sa_session.commit()
 
@@ -4034,8 +4030,7 @@ def store_by(self):
         return store_by
 
     def extra_files_path_name_from(self, object_store):
-        store_by = self.store_by
-        if store_by is not None:
+        if (store_by := self.store_by) is not None:
             return f"dataset_{getattr(self, store_by)}_files"
         else:
             return None
@@ -4107,8 +4102,7 @@ def set_total_size(self):
         if self.file_size is None:
             self.set_size()
         self.total_size = self.file_size or 0
-        rel_path = self._extra_files_rel_path
-        if rel_path is not None:
+        if (rel_path := self._extra_files_rel_path) is not None:
             if self.object_store.exists(self, extra_dir=rel_path, dir_only=True):
                 for root, _, files in os.walk(self.extra_files_path):
                     self.total_size += sum(
@@ -4145,8 +4139,7 @@ def full_delete(self):
             self.object_store.delete(self)
         except galaxy.exceptions.ObjectNotFound:
             pass
-        rel_path = self._extra_files_rel_path
-        if rel_path is not None:
+        if (rel_path := self._extra_files_rel_path) is not None:
             if self.object_store.exists(self, extra_dir=rel_path, dir_only=True):
                 self.object_store.delete(self, entire_dir=True, extra_dir=rel_path, dir_only=True)
         # TODO: purge metadata files
@@ -7863,8 +7856,7 @@ def copy_to(self, copied_step, step_mapping, user=None):
                 annotations.append(association)
             copied_step.annotations = annotations
 
-        subworkflow = self.subworkflow
-        if subworkflow:
+        if subworkflow := self.subworkflow:
             copied_subworkflow = subworkflow.copy()
             copied_step.subworkflow = copied_subworkflow
             for subworkflow_step, copied_subworkflow_step in zip(subworkflow.steps, copied_subworkflow.steps):
@@ -8676,8 +8668,7 @@ def set_handler(self, handler):
 
     def log_str(self):
         extra = ""
-        safe_id = getattr(self, "id", None)
-        if safe_id is not None:
+        if (safe_id := getattr(self, "id", None)) is not None:
             extra += f"id={safe_id}"
         else:
             extra += "unflushed"
@@ -9622,8 +9613,7 @@ def load(cls, token):
 
     @classmethod
     def destroy(cls, token):
-        partial = cls.load(token)
-        if partial:
+        if partial := cls.load(token):
             session = cls.sa_session
             session.execute(delete(partial))
             with transaction(session):
diff --git a/lib/galaxy/model/dataset_collections/types/paired.py b/lib/galaxy/model/dataset_collections/types/paired.py
index 3ac8d7da4d89..cfbd19c04343 100644
--- a/lib/galaxy/model/dataset_collections/types/paired.py
+++ b/lib/galaxy/model/dataset_collections/types/paired.py
@@ -19,15 +19,13 @@ def __init__(self):
         pass
 
     def generate_elements(self, elements):
-        forward_dataset = elements.get(FORWARD_IDENTIFIER)
-        reverse_dataset = elements.get(REVERSE_IDENTIFIER)
-        if forward_dataset:
+        if forward_dataset := elements.get(FORWARD_IDENTIFIER):
             left_association = DatasetCollectionElement(
                 element=forward_dataset,
                 element_identifier=FORWARD_IDENTIFIER,
             )
             yield left_association
-        if reverse_dataset:
+        if reverse_dataset := elements.get(REVERSE_IDENTIFIER):
             right_association = DatasetCollectionElement(
                 element=reverse_dataset,
                 element_identifier=REVERSE_IDENTIFIER,
diff --git a/lib/galaxy/model/item_attrs.py b/lib/galaxy/model/item_attrs.py
index 998536e07b7d..e361d5458441 100644
--- a/lib/galaxy/model/item_attrs.py
+++ b/lib/galaxy/model/item_attrs.py
@@ -96,8 +96,7 @@ def add_item_annotation(self, db_session, user, item, annotation):
         return add_item_annotation(db_session, user, item, annotation)
 
     def delete_item_annotation(self, db_session, user, item):
-        annotation_assoc = get_item_annotation_obj(db_session, user, item)
-        if annotation_assoc:
+        if annotation_assoc := get_item_annotation_obj(db_session, user, item):
             db_session.delete(annotation_assoc)
             with transaction(db_session):
                 db_session.commit()
diff --git a/lib/galaxy/model/store/__init__.py b/lib/galaxy/model/store/__init__.py
index e8a6c95fb377..e18fa9ba71a5 100644
--- a/lib/galaxy/model/store/__init__.py
+++ b/lib/galaxy/model/store/__init__.py
@@ -1968,8 +1968,7 @@ def push_metadata_files(self):
 
     def export_job(self, job: model.Job, tool=None, include_job_data=True):
         self.export_jobs([job], include_job_data=include_job_data)
-        tool_source = getattr(tool, "tool_source", None)
-        if tool_source:
+        if tool_source := getattr(tool, "tool_source", None):
             with open(os.path.join(self.export_directory, "tool.xml"), "w") as out:
                 out.write(tool_source.to_string())
 
@@ -2288,8 +2287,7 @@ def record_job(job):
                     return
 
                 jobs_dict[job.id] = job
-                icja = job.implicit_collection_jobs_association
-                if icja:
+                if icja := job.implicit_collection_jobs_association:
                     implicit_collection_jobs = icja.implicit_collection_jobs
                     implicit_collection_jobs_dict[implicit_collection_jobs.id] = implicit_collection_jobs
 
diff --git a/lib/galaxy/model/store/discover.py b/lib/galaxy/model/store/discover.py
index c08579cc3725..ae7143a5ff47 100644
--- a/lib/galaxy/model/store/discover.py
+++ b/lib/galaxy/model/store/discover.py
@@ -1004,8 +1004,7 @@ def __init__(self, as_dict, collector: Optional[CollectorT], filename, path=None
     def designation(self):
         # If collecting nested collection, grab identifier_0,
         # identifier_1, etc... and join on : to build designation.
-        element_identifiers = self.raw_element_identifiers
-        if element_identifiers:
+        if element_identifiers := self.raw_element_identifiers:
             return ":".join(element_identifiers)
         elif "designation" in self.as_dict:
             return self.as_dict.get("designation")
diff --git a/lib/galaxy/model/store/load_objects.py b/lib/galaxy/model/store/load_objects.py
index 3c3fbed2bc82..7666187764ca 100644
--- a/lib/galaxy/model/store/load_objects.py
+++ b/lib/galaxy/model/store/load_objects.py
@@ -35,8 +35,7 @@ def main(argv=None):
     api_url = f"{galaxy_url.rstrip('/')}/api"
     api_key = args.key
     assert api_key
-    history_id = args.history_id
-    if history_id:
+    if history_id := args.history_id:
         create_url = f"{api_url}/histories/{history_id}/contents_from_store?key={api_key}"
     else:
         create_url = f"{api_url}/histories/from_store?key={api_key}"
diff --git a/lib/galaxy/model/tags.py b/lib/galaxy/model/tags.py
index 739d8528195d..a62051b79eef 100644
--- a/lib/galaxy/model/tags.py
+++ b/lib/galaxy/model/tags.py
@@ -147,9 +147,8 @@ def remove_item_tag(self, user: "User", item, tag_name: str):
         """Remove a tag from an item."""
         self._ensure_user_owns_item(user, item)
         # Get item tag association.
-        item_tag_assoc = self._get_item_tag_assoc(user, item, tag_name)
         # Remove association.
-        if item_tag_assoc:
+        if item_tag_assoc := self._get_item_tag_assoc(user, item, tag_name):
             # Delete association.
             self.sa_session.delete(item_tag_assoc)
             item.tags.remove(item_tag_assoc)
diff --git a/lib/galaxy/model/tool_shed_install/__init__.py b/lib/galaxy/model/tool_shed_install/__init__.py
index d406935c79fb..1d41d5c3c677 100644
--- a/lib/galaxy/model/tool_shed_install/__init__.py
+++ b/lib/galaxy/model/tool_shed_install/__init__.py
@@ -242,10 +242,9 @@ def get_shed_config_dict(self, app: HasToolBox) -> DynamicToolConfDict:
 
     def get_tool_relative_path(self, app: HasToolBox):
         # This is a somewhat public function, used by data_manager_manual for instance
-        shed_conf_dict = self.get_shed_config_dict(app)
         tool_path = None
         relative_path = None
-        if shed_conf_dict:
+        if shed_conf_dict := self.get_shed_config_dict(app):
             tool_path = shed_conf_dict["tool_path"]
             relative_path = os.path.join(
                 self.tool_shed_path_name, "repos", self.owner, self.name, self.installed_changeset_revision
@@ -411,8 +410,7 @@ def missing_tool_dependencies(self):
         return missing_dependencies
 
     def repo_files_directory(self, app: HasToolBox):
-        repo_path = self.repo_path(app)
-        if repo_path:
+        if repo_path := self.repo_path(app):
             return os.path.join(repo_path, self.name)
         return None
 
diff --git a/lib/galaxy/model/unittest_utils/model_testing_utils.py b/lib/galaxy/model/unittest_utils/model_testing_utils.py
index f67a5bf81d64..4f0797143612 100644
--- a/lib/galaxy/model/unittest_utils/model_testing_utils.py
+++ b/lib/galaxy/model/unittest_utils/model_testing_utils.py
@@ -97,8 +97,7 @@ def url_factory(tmp_directory: str) -> Callable[[], DbUrl]:
 
     def url() -> DbUrl:
         database = _generate_unique_database_name()
-        connection_url = _get_connection_url()
-        if connection_url:
+        if connection_url := _get_connection_url():
             return _make_postgres_db_url(DbUrl(connection_url), database)
         else:
             return _make_sqlite_db_url(tmp_directory, database)
@@ -115,8 +114,7 @@ def url(tmp_directory: str) -> str:
     """
     # TODO this duplication should be removed (see url_factory).
     database = _generate_unique_database_name()
-    connection_url = _get_connection_url()
-    if connection_url:
+    if connection_url := _get_connection_url():
         return _make_postgres_db_url(DbUrl(connection_url), database)
     else:
         return _make_sqlite_db_url(tmp_directory, database)
diff --git a/lib/galaxy/navigation/components.py b/lib/galaxy/navigation/components.py
index 34b677a7e16b..77da115784ef 100644
--- a/lib/galaxy/navigation/components.py
+++ b/lib/galaxy/navigation/components.py
@@ -255,8 +255,7 @@ def resolve_component_locator(self, path: Optional[str] = None) -> LocatorT:
 
         def arguments() -> Tuple[str, Optional[Dict[str, str]], Optional[str]]:
             assert path
-            match = CALL_ARGUMENTS_RE.match(path)
-            if match:
+            if match := CALL_ARGUMENTS_RE.match(path):
                 component_name = match.group("SUBCOMPONENT")
                 expression = match.group("ARGS")
                 rest = match.group("REST")
diff --git a/lib/galaxy/queue_worker.py b/lib/galaxy/queue_worker.py
index e133a9ee399b..3d80addf28dd 100644
--- a/lib/galaxy/queue_worker.py
+++ b/lib/galaxy/queue_worker.py
@@ -216,9 +216,8 @@ def reload_sanitize_allowlist(app):
 
 
 def recalculate_user_disk_usage(app, **kwargs):
-    user_id = kwargs.get("user_id", None)
     sa_session = app.model.context
-    if user_id:
+    if user_id := kwargs.get("user_id", None):
         user = sa_session.get(User, user_id)
         if user:
             user.calculate_and_set_disk_usage(app.object_store)
diff --git a/lib/galaxy/schema/drs/Checksum.py b/lib/galaxy/schema/drs/Checksum.py
index 24cae8b6cac2..afa32f958e05 100644
--- a/lib/galaxy/schema/drs/Checksum.py
+++ b/lib/galaxy/schema/drs/Checksum.py
@@ -14,6 +14,6 @@ class Model(BaseModel):
     checksum: str = Field(..., description="The hex-string encoded checksum for the data")
     type: str = Field(
         ...,
-        description="The digest method used to create the checksum.\nThe value (e.g. `sha-256`) SHOULD be listed as `Hash Name String` in the https://www.iana.org/assignments/named-information/named-information.xhtml#hash-alg[IANA Named Information Hash Algorithm Registry]. Other values MAY be used, as long as implementors are aware of the issues discussed in https://tools.ietf.org/html/rfc6920#section-9.4[RFC6920].\nGA4GH may provide more explicit guidance for use of non-IANA-registered algorithms in the future. Until then, if implementors do choose such an algorithm (e.g. because it's implemented by their storage provider), they SHOULD use an existing standard `type` value such as `md5`, `etag`, `crc32c`, `trunc512`, or `sha1`.",
+        description="The digest method used to create the checksum.\nThe value (e.g. `sha-256`) SHOULD be listed as `Hash Name String` in the https://www.iana.org/assignments/named-information/named-information.xhtml#hash-alg[IANA Named Information Hash Algorithm Registry]. Other values MAY be used, as long as implementers are aware of the issues discussed in https://tools.ietf.org/html/rfc6920#section-9.4[RFC6920].\nGA4GH may provide more explicit guidance for use of non-IANA-registered algorithms in the future. Until then, if implementers do choose such an algorithm (e.g. because it's implemented by their storage provider), they SHOULD use an existing standard `type` value such as `md5`, `etag`, `crc32c`, `trunc512`, or `sha1`.",
         example="sha-256",
     )
diff --git a/lib/galaxy/schema/drs/DrsObject.py b/lib/galaxy/schema/drs/DrsObject.py
index 98c98ba36339..21e8e14fc088 100644
--- a/lib/galaxy/schema/drs/DrsObject.py
+++ b/lib/galaxy/schema/drs/DrsObject.py
@@ -21,7 +21,7 @@ class Checksum(BaseModel):
     checksum: str = Field(..., description="The hex-string encoded checksum for the data")
     type: str = Field(
         ...,
-        description="The digest method used to create the checksum.\nThe value (e.g. `sha-256`) SHOULD be listed as `Hash Name String` in the https://www.iana.org/assignments/named-information/named-information.xhtml#hash-alg[IANA Named Information Hash Algorithm Registry]. Other values MAY be used, as long as implementors are aware of the issues discussed in https://tools.ietf.org/html/rfc6920#section-9.4[RFC6920].\nGA4GH may provide more explicit guidance for use of non-IANA-registered algorithms in the future. Until then, if implementors do choose such an algorithm (e.g. because it's implemented by their storage provider), they SHOULD use an existing standard `type` value such as `md5`, `etag`, `crc32c`, `trunc512`, or `sha1`.",
+        description="The digest method used to create the checksum.\nThe value (e.g. `sha-256`) SHOULD be listed as `Hash Name String` in the https://www.iana.org/assignments/named-information/named-information.xhtml#hash-alg[IANA Named Information Hash Algorithm Registry]. Other values MAY be used, as long as implementers are aware of the issues discussed in https://tools.ietf.org/html/rfc6920#section-9.4[RFC6920].\nGA4GH may provide more explicit guidance for use of non-IANA-registered algorithms in the future. Until then, if implementers do choose such an algorithm (e.g. because it's implemented by their storage provider), they SHOULD use an existing standard `type` value such as `md5`, `etag`, `crc32c`, `trunc512`, or `sha1`.",
         example="sha-256",
     )
 
diff --git a/lib/galaxy/security/object_wrapper.py b/lib/galaxy/security/object_wrapper.py
index 15352cfa9fdd..1c7176a29b16 100644
--- a/lib/galaxy/security/object_wrapper.py
+++ b/lib/galaxy/security/object_wrapper.py
@@ -165,8 +165,7 @@ def __do_wrap(value):
         except Exception:
             wrapped_class_name = value.__class__.__name__
             wrapped_class = value.__class__
-        value_mod = inspect.getmodule(value)
-        if value_mod:
+        if value_mod := inspect.getmodule(value):
             wrapped_class_name = f"{value_mod.__name__}.{wrapped_class_name}"
         wrapped_class_name = (
             f"SafeStringWrapper__{wrapped_class_name}__{'__'.join(sorted(c.__name__ for c in no_wrap_classes))}"
diff --git a/lib/galaxy/security/validate_user_input.py b/lib/galaxy/security/validate_user_input.py
index a5400bd0ee02..29e052db4dc3 100644
--- a/lib/galaxy/security/validate_user_input.py
+++ b/lib/galaxy/security/validate_user_input.py
@@ -129,8 +129,7 @@ def validate_publicname(trans, publicname, user=None):
     """
     if user and user.username == publicname:
         return ""
-    message = validate_publicname_str(publicname)
-    if message:
+    if message := validate_publicname_str(publicname):
         return message
 
     stmt = select(trans.app.model.User).filter_by(username=publicname).limit(1)
diff --git a/lib/galaxy/selenium/axe_results.py b/lib/galaxy/selenium/axe_results.py
index 3f2707644348..fae862c61812 100644
--- a/lib/galaxy/selenium/axe_results.py
+++ b/lib/galaxy/selenium/axe_results.py
@@ -107,8 +107,7 @@ def assert_passes(self, id: str) -> None:
 
     def assert_does_not_violate(self, id: str) -> None:
         violations = self._json["violations"]
-        result = _check_list_for_id(violations, id)
-        if result:
+        if result := _check_list_for_id(violations, id):
             violation = Violation(result)
             raise AssertionError(violation.message)
 
diff --git a/lib/galaxy/selenium/has_driver.py b/lib/galaxy/selenium/has_driver.py
index 5792cec3d6fd..de7f904edcbd 100644
--- a/lib/galaxy/selenium/has_driver.py
+++ b/lib/galaxy/selenium/has_driver.py
@@ -286,8 +286,7 @@ def prepend_timeout_message(
         self, timeout_exception: SeleniumTimeoutException, message: str
     ) -> SeleniumTimeoutException:
         msg = message
-        timeout_msg = timeout_exception.msg
-        if timeout_msg:
+        if timeout_msg := timeout_exception.msg:
             msg += f" {timeout_msg}"
         return SeleniumTimeoutException(
             msg=msg,
diff --git a/lib/galaxy/selenium/navigates_galaxy.py b/lib/galaxy/selenium/navigates_galaxy.py
index f156f348703d..246955f8fc50 100644
--- a/lib/galaxy/selenium/navigates_galaxy.py
+++ b/lib/galaxy/selenium/navigates_galaxy.py
@@ -564,10 +564,9 @@ def published_grid_search_for(self, search_term=None):
         )
 
     def get_logged_in_user(self) -> Optional[Dict[str, Any]]:
-        user_dict = self.api_get("users/current")
         # for user's not logged in - this just returns a {} so lets
         # key this on an id being available?
-        if "id" in user_dict:
+        if "id" in (user_dict := self.api_get("users/current")):
             return user_dict
         else:
             return None
@@ -586,8 +585,7 @@ def get_api_key(self, force=False) -> Optional[str]:
             return self.api_post(f"users/{user_id}/api_key")
 
     def get_user_id(self) -> Optional[str]:
-        user = self.get_logged_in_user()
-        if user is not None:
+        if (user := self.get_logged_in_user()) is not None:
             return user["id"]
         else:
             return None
@@ -2076,8 +2074,7 @@ def run_tour_step(self, step, step_index, tour_callback):
             element = self.tour_wait_for_element_present(element_str)
             assert element is not None
 
-        textinsert = step.get("textinsert", None)
-        if textinsert is not None:
+        if (textinsert := step.get("textinsert", None)) is not None:
             element.send_keys(textinsert)
 
         tour_callback.handle_step(step, step_index)
diff --git a/lib/galaxy/selenium/sizzle.py b/lib/galaxy/selenium/sizzle.py
index 8716a2c55b92..94d9e903b6ae 100644
--- a/lib/galaxy/selenium/sizzle.py
+++ b/lib/galaxy/selenium/sizzle.py
@@ -68,8 +68,7 @@ def find_element_by_sizzle(driver, sizzle_selector: str):
 
     :param sizzle_selector: The sizzle selector to use when finding element.
     """
-    elements = driver.find_elements_by_sizzle(sizzle_selector)
-    if elements:
+    if elements := driver.find_elements_by_sizzle(sizzle_selector):
         return elements[0]
     else:
         raise NoSuchElementException(f"Unable to locate element by Sizzle: {sizzle_selector}")
diff --git a/lib/galaxy/selenium/smart_components.py b/lib/galaxy/selenium/smart_components.py
index 9400a706f1aa..9a6c2c61b257 100644
--- a/lib/galaxy/selenium/smart_components.py
+++ b/lib/galaxy/selenium/smart_components.py
@@ -125,8 +125,7 @@ def data_value(self, attribute: str):
         return attribute_value
 
     def assert_data_value(self, attribute: str, expected_value: str):
-        actual_value = self.data_value(attribute)
-        if actual_value != expected_value:
+        if (actual_value := self.data_value(attribute)) != expected_value:
             message = f"Expected data-{attribute} to have value [{expected_value}] but had value [{actual_value}]"
             raise AssertionError(message)
 
diff --git a/lib/galaxy/tool_shed/galaxy_install/metadata/installed_repository_metadata_manager.py b/lib/galaxy/tool_shed/galaxy_install/metadata/installed_repository_metadata_manager.py
index f8fa39bf80bb..aea14cacdd93 100644
--- a/lib/galaxy/tool_shed/galaxy_install/metadata/installed_repository_metadata_manager.py
+++ b/lib/galaxy/tool_shed/galaxy_install/metadata/installed_repository_metadata_manager.py
@@ -152,10 +152,9 @@ def reset_metadata_on_selected_repositories(self, user, **kwd):
         Inspect the repository changelog to reset metadata for all appropriate changeset revisions.
         This method is called from both Galaxy and the Tool Shed.
         """
-        repository_ids = util.listify(kwd.get("repository_ids", None))
         message = ""
         status = "done"
-        if repository_ids:
+        if repository_ids := util.listify(kwd.get("repository_ids", None)):
             successful_count = 0
             unsuccessful_count = 0
             for repository_id in repository_ids:
diff --git a/lib/galaxy/tool_shed/galaxy_install/tools/data_manager.py b/lib/galaxy/tool_shed/galaxy_install/tools/data_manager.py
index d3cce73744b5..f2cbcdc072af 100644
--- a/lib/galaxy/tool_shed/galaxy_install/tools/data_manager.py
+++ b/lib/galaxy/tool_shed/galaxy_install/tools/data_manager.py
@@ -52,8 +52,7 @@ def _data_manager_config_elems_to_xml_file(self, config_elems: List[Element], co
         Persist the current in-memory list of config_elems to a file named by the value
         of config_filename.
         """
-        data_managers_path = self.data_managers_path
-        if data_managers_path:
+        if data_managers_path := self.data_managers_path:
             root_str = f'<?xml version="1.0"?><data_managers tool_path="{data_managers_path}"></data_managers>'
         else:
             root_str = '<?xml version="1.0"?><data_managers></data_managers>'
diff --git a/lib/galaxy/tool_shed/galaxy_install/tools/tool_panel_manager.py b/lib/galaxy/tool_shed/galaxy_install/tools/tool_panel_manager.py
index c68ba7fe4be3..e4d1dc3c19bd 100644
--- a/lib/galaxy/tool_shed/galaxy_install/tools/tool_panel_manager.py
+++ b/lib/galaxy/tool_shed/galaxy_install/tools/tool_panel_manager.py
@@ -243,10 +243,9 @@ def generate_tool_panel_dict_from_shed_tool_conf_entries(self, repository) -> Di
         shed_tool_conf, tool_path, relative_install_dir = get_tool_panel_config_tool_path_install_dir(
             self.app, repository
         )
-        metadata = repository.metadata_
         # Create a dictionary of tool guid and tool config file name for each tool in the repository.
         guids_and_configs = {}
-        if "tools" in metadata:
+        if "tools" in (metadata := repository.metadata_):
             for tool_dict in metadata["tools"]:
                 guid = tool_dict["guid"]
                 tool_config = tool_dict["tool_config"]
diff --git a/lib/galaxy/tool_shed/metadata/metadata_generator.py b/lib/galaxy/tool_shed/metadata/metadata_generator.py
index d797d1eba98c..71d3f666f7ad 100644
--- a/lib/galaxy/tool_shed/metadata/metadata_generator.py
+++ b/lib/galaxy/tool_shed/metadata/metadata_generator.py
@@ -135,8 +135,7 @@ def _generate_data_manager_metadata(
                 tool_conf_name = os.path.join(tool_path, tool_conf_name)
             tools[tool_conf_name] = tool
         root = tree.getroot()
-        data_manager_tool_path = root.get("tool_path", None)
-        if data_manager_tool_path:
+        if data_manager_tool_path := root.get("tool_path", None):
             relative_data_manager_dir = os.path.join(relative_data_manager_dir, data_manager_tool_path)
         for i, data_manager_elem in enumerate(root.findall("data_manager")):
             tool_file = data_manager_elem.get("tool_file", None)
diff --git a/lib/galaxy/tool_shed/util/dependency_display.py b/lib/galaxy/tool_shed/util/dependency_display.py
index f4c2ef30a60a..61b427718250 100644
--- a/lib/galaxy/tool_shed/util/dependency_display.py
+++ b/lib/galaxy/tool_shed/util/dependency_display.py
@@ -197,8 +197,7 @@ def populate_containers_dict_from_repository_metadata(self, repository):
         when displaying repository dependencies for installed repositories and when displaying
         them for uninstalled repositories that are being reinstalled.
         """
-        metadata = repository.metadata_
-        if metadata:
+        if metadata := repository.metadata_:
             irm = InstalledRepositoryManager(self.app)
             # Handle repository dependencies.
             (
diff --git a/lib/galaxy/tool_util/deps/conda_util.py b/lib/galaxy/tool_util/deps/conda_util.py
index f91203923cd2..9808bd788010 100644
--- a/lib/galaxy/tool_util/deps/conda_util.py
+++ b/lib/galaxy/tool_util/deps/conda_util.py
@@ -254,7 +254,7 @@ def exec_command(self, operation: str, args: List[str], stdout_path: Optional[st
         if self.condarc_override:
             env["CONDARC"] = self.condarc_override
         cmd_string = shlex_join(cmd)
-        kwds = dict()
+        kwds: Dict[str, Any] = dict()
         try:
             if stdout_path:
                 kwds["stdout"] = open(stdout_path, "w")
diff --git a/lib/galaxy/tools/__init__.py b/lib/galaxy/tools/__init__.py
index 3168f6a52e13..d94f5b4d3c0f 100644
--- a/lib/galaxy/tools/__init__.py
+++ b/lib/galaxy/tools/__init__.py
@@ -340,8 +340,7 @@ class ToolNotFoundException(Exception):
 
 def create_tool_from_source(app, tool_source, config_file=None, **kwds):
     # Allow specifying a different tool subclass to instantiate
-    tool_module = tool_source.parse_tool_module()
-    if tool_module is not None:
+    if (tool_module := tool_source.parse_tool_module()) is not None:
         module, cls = tool_module
         mod = __import__(module, globals(), locals(), [cls])
         ToolClass = getattr(mod, cls)
@@ -444,8 +443,7 @@ def __init__(self, config_filenames, tool_root_dir, app, save_integrated_tool_pa
             save_integrated_tool_panel=save_integrated_tool_panel,
         )
 
-        old_toolbox = getattr(app, "toolbox", None)
-        if old_toolbox:
+        if old_toolbox := getattr(app, "toolbox", None):
             self.dependency_manager = old_toolbox.dependency_manager
         else:
             self._init_dependency_manager()
@@ -826,8 +824,7 @@ def __init__(
             self.job_search = self.app.job_search
 
     def remove_from_cache(self):
-        source_path = self.tool_source.source_path
-        if source_path:
+        if source_path := self.tool_source.source_path:
             for region in self.app.toolbox.cache_regions.values():
                 region.delete(source_path)
 
@@ -1093,8 +1090,7 @@ def parse(self, tool_source: ToolSource, guid=None, dynamic=False):
 
         # Versioning for tools
         self.version_string_cmd = None
-        version_command = tool_source.parse_version_command()
-        if version_command is not None:
+        if (version_command := tool_source.parse_version_command()) is not None:
             self.version_string_cmd = version_command.strip()
 
             version_cmd_interpreter = tool_source.parse_version_command_interpreter()
@@ -1261,8 +1257,7 @@ def __parse_legacy_features(self, tool_source):
                         raise
 
         # User interface hints
-        uihints_elem = root.find("uihints")
-        if uihints_elem is not None:
+        if (uihints_elem := root.find("uihints")) is not None:
             for key, value in uihints_elem.attrib.items():
                 self.uihints[key] = value
 
@@ -1272,8 +1267,7 @@ def __parse_config_files(self, tool_source):
             return
 
         root = tool_source.root
-        conf_parent_elem = root.find("configfiles")
-        if conf_parent_elem is not None:
+        if (conf_parent_elem := root.find("configfiles")) is not None:
             inputs_elem = conf_parent_elem.find("inputs")
             if inputs_elem is not None:
                 name = inputs_elem.get("name")
@@ -1300,13 +1294,11 @@ def __parse_trackster_conf(self, tool_source):
             return
 
         # Trackster configuration.
-        trackster_conf = tool_source.root.find("trackster_conf")
-        if trackster_conf is not None:
+        if (trackster_conf := tool_source.root.find("trackster_conf")) is not None:
             self.trackster_conf = TracksterConfig.parse(trackster_conf)
 
     def parse_tests(self):
-        tests_source = self.tool_source
-        if tests_source:
+        if tests_source := self.tool_source:
             try:
                 self.__tests = json.dumps([t.to_dict() for t in parse_tests(self, tests_source)], indent=None)
             except Exception:
@@ -1335,9 +1327,8 @@ def _repository_dir(self):
         return repository_base_dir
 
     def test_data_path(self, filename):
-        repository_dir = self._repository_dir
         test_data = None
-        if repository_dir:
+        if repository_dir := self._repository_dir:
             test_data = self.__walk_test_data(dir=repository_dir, filename=filename)
         else:
             if self.tool_dir:
@@ -1380,8 +1371,7 @@ def tool_provided_metadata(self, job_wrapper):
     def parse_command(self, tool_source):
         """ """
         # Command line (template). Optional for tools that do not invoke a local program
-        command = tool_source.parse_command()
-        if command is not None:
+        if (command := tool_source.parse_command()) is not None:
             self.command = command.lstrip()  # get rid of leading whitespace
             # Must pre-pend this AFTER processing the cheetah command template
             self.interpreter = tool_source.parse_interpreter()
@@ -1637,8 +1627,7 @@ def parse_param_elem(self, input_source: InputSource, enctypes, context) -> Tool
         enctypes.
         """
         param = ToolParameter.build(self, input_source)
-        param_enctype = param.get_required_enctype()
-        if param_enctype:
+        if param_enctype := param.get_required_enctype():
             enctypes.add(param_enctype)
         # If parameter depends on any other paramters, we must refresh the
         # form when it changes
@@ -2343,8 +2332,7 @@ def to_archive(self):
         if os.path.exists(os.path.join(tool_path, "Dockerfile")):
             tarball_files.append((os.path.join(tool_path, "Dockerfile"), "Dockerfile"))
         # Find tests, and check them for test data.
-        tests = tool.tests
-        if tests is not None:
+        if (tests := tool.tests) is not None:
             for test in tests:
                 # Add input file tuples to the list.
                 for input in test.inputs:
@@ -2651,8 +2639,7 @@ def map_to_history(value):
                 source = hdca_source_dict
             else:
                 return None
-            key = f"{value.hid}_{id}"
-            if key in source:
+            if (key := f"{value.hid}_{id}") in source:
                 return source[key]
             elif id in source:
                 return source[id]
@@ -3373,10 +3360,9 @@ def produce_outputs(self, trans, out_data, output_collections, incoming, history
         for incoming_repeat in incoming["inputs"]:
             input_lists.append(incoming_repeat["input"])
 
-        advanced = incoming.get("advanced", None)
         dupl_actions = "keep_first"
         suffix_pattern = None
-        if advanced is not None:
+        if (advanced := incoming.get("advanced", None)) is not None:
             dupl_actions = advanced["conflict"]["duplicate_options"]
 
             if dupl_actions in ["suffix_conflict", "suffix_every", "suffix_conflict_rest"]:
diff --git a/lib/galaxy/tools/actions/model_operations.py b/lib/galaxy/tools/actions/model_operations.py
index 5b71f55c0542..a1e7230b9eeb 100644
--- a/lib/galaxy/tools/actions/model_operations.py
+++ b/lib/galaxy/tools/actions/model_operations.py
@@ -126,8 +126,7 @@ def _produce_outputs(
             hdca_tags=hdca_tags,
             tag_handler=tag_handler,
         )
-        mapped_over_elements = output_collections.dataset_collection_elements
-        if mapped_over_elements:
+        if mapped_over_elements := output_collections.dataset_collection_elements:
             for name, value in out_data.items():
                 if name in mapped_over_elements:
                     value.visible = False
diff --git a/lib/galaxy/tools/actions/upload_common.py b/lib/galaxy/tools/actions/upload_common.py
index 696d21b8de38..a345abde954a 100644
--- a/lib/galaxy/tools/actions/upload_common.py
+++ b/lib/galaxy/tools/actions/upload_common.py
@@ -196,8 +196,7 @@ def __new_library_upload(trans, cntrller, uploaded_dataset, library_bunch, tag_h
         tag_from_filename = os.path.splitext(os.path.basename(uploaded_dataset.name))[0]
         tag_handler.apply_item_tag(item=ldda, user=trans.user, name="name", value=tag_from_filename, flush=False)
 
-    tags_list = uploaded_dataset.get("tags", False)
-    if tags_list:
+    if tags_list := uploaded_dataset.get("tags", False):
         for tag in tags_list:
             tag_handler.apply_item_tag(item=ldda, user=trans.user, name="name", value=tag, flush=False)
 
diff --git a/lib/galaxy/tools/data_fetch.py b/lib/galaxy/tools/data_fetch.py
index e3db469dc99f..19e272ca4b6b 100644
--- a/lib/galaxy/tools/data_fetch.py
+++ b/lib/galaxy/tools/data_fetch.py
@@ -90,9 +90,8 @@ def _fetch_target(upload_config: "UploadConfig", target):
     assert destination, "No destination defined."
 
     def expand_elements_from(target_or_item):
-        elements_from = target_or_item.get("elements_from", None)
         items = None
-        if elements_from:
+        if elements_from := target_or_item.get("elements_from", None):
             if elements_from == "archive":
                 decompressed_directory = _decompress_target(upload_config, target_or_item)
                 items = _directory_to_items(decompressed_directory)
@@ -139,11 +138,10 @@ def _copy_and_validate_simple_attributes(src_item, target_metadata):
         info = src_item.get("info", None)
         created_from_basename = src_item.get("created_from_basename", None)
         tags = src_item.get("tags", [])
-        object_id = src_item.get("object_id", None)
 
         if info is not None:
             target_metadata["info"] = info
-        if object_id is not None:
+        if (object_id := src_item.get("object_id", None)) is not None:
             target_metadata["object_id"] = object_id
         if tags:
             target_metadata["tags"] = tags
diff --git a/lib/galaxy/tools/error_reports/plugins/gitlab.py b/lib/galaxy/tools/error_reports/plugins/gitlab.py
index 9127deb2943c..f85c3b2fd2e0 100644
--- a/lib/galaxy/tools/error_reports/plugins/gitlab.py
+++ b/lib/galaxy/tools/error_reports/plugins/gitlab.py
@@ -247,8 +247,7 @@ def _create_issue(self, issue_cache_key, error_title, error_message, project, **
         issue_data = {"title": error_title, "description": error_message}
 
         # Assign the user to the issue
-        gl_userid = kwargs.get("gl_userid", None)
-        if gl_userid is not None:
+        if (gl_userid := kwargs.get("gl_userid", None)) is not None:
             issue_data["assignee_ids"] = [gl_userid]
 
         # Create the issue on GitLab
diff --git a/lib/galaxy/tools/evaluation.py b/lib/galaxy/tools/evaluation.py
index d06af3b01d53..2e0c40c2c3ff 100644
--- a/lib/galaxy/tools/evaluation.py
+++ b/lib/galaxy/tools/evaluation.py
@@ -613,8 +613,7 @@ def _build_command_line(self):
         self.command_line = command_line
 
     def _build_version_command(self):
-        version_string_cmd_raw = self.tool.version_string_cmd
-        if version_string_cmd_raw:
+        if version_string_cmd_raw := self.tool.version_string_cmd:
             version_command_template = string.Template(version_string_cmd_raw)
             version_command = version_command_template.safe_substitute(
                 {"__tool_directory__": self.compute_environment.tool_directory()}
@@ -694,12 +693,10 @@ def _build_environment_variables(self):
             environment_variable["job_directory_path"] = config_filename
             environment_variables.append(environment_variable)
 
-        home_dir = self.compute_environment.home_directory()
-        tmp_dir = self.compute_environment.tmp_directory()
-        if home_dir:
+        if home_dir := self.compute_environment.home_directory():
             environment_variable = dict(name="HOME", value=f'"{home_dir}"', raw=True)
             environment_variables.append(environment_variable)
-        if tmp_dir:
+        if tmp_dir := self.compute_environment.tmp_directory():
             for tmp_directory_var in self.tool.tmp_directory_vars:
                 environment_variable = dict(name=tmp_directory_var, value=f'"{tmp_dir}"', raw=True)
                 environment_variables.append(environment_variable)
@@ -806,8 +803,7 @@ def _history(self):
 
     @property
     def _user(self):
-        history = self._history
-        if history:
+        if history := self._history:
             return history.user
         else:
             return self.job.user
diff --git a/lib/galaxy/tools/execute.py b/lib/galaxy/tools/execute.py
index 26d29598c740..e1ac8f9f6e25 100644
--- a/lib/galaxy/tools/execute.py
+++ b/lib/galaxy/tools/execute.py
@@ -140,8 +140,7 @@ def execute_single_job(execution_slice, completed_job, skip=False):
             execution_tracker.record_error(result)
 
     tool_action = tool.tool_action
-    check_inputs_ready = getattr(tool_action, "check_inputs_ready", None)
-    if check_inputs_ready:
+    if check_inputs_ready := getattr(tool_action, "check_inputs_ready", None):
         for params in execution_tracker.param_combinations:
             # This will throw an exception if the tool is not ready.
             try:
diff --git a/lib/galaxy/tools/imp_exp/export_history.py b/lib/galaxy/tools/imp_exp/export_history.py
index 4127d11770bb..340c19a203d4 100644
--- a/lib/galaxy/tools/imp_exp/export_history.py
+++ b/lib/galaxy/tools/imp_exp/export_history.py
@@ -40,9 +40,6 @@ def main(argv=None):
     )
     parser.add_option("--file-sources", type=str, help="file sources json")
     (options, args) = parser.parse_args(argv)
-    galaxy_version = options.galaxy_version
-    if galaxy_version is None:
-        galaxy_version = "19.05"
 
     gzip = bool(options.gzip)
     assert len(args) >= 2
diff --git a/lib/galaxy/tools/parameters/basic.py b/lib/galaxy/tools/parameters/basic.py
index ef6a7a19613c..af3e537a1beb 100644
--- a/lib/galaxy/tools/parameters/basic.py
+++ b/lib/galaxy/tools/parameters/basic.py
@@ -110,8 +110,7 @@ def is_runtime_context(trans, other_values):
 
 
 def parse_dynamic_options(param, input_source):
-    options_elem = input_source.parse_dynamic_options_elem()
-    if options_elem is not None:
+    if (options_elem := input_source.parse_dynamic_options_elem()) is not None:
         return dynamic_options.DynamicOptions(options_elem, param)
     return None
 
@@ -180,8 +179,7 @@ def __init__(self, tool, input_source, context=None):
         self.is_dynamic = False
         self.label = input_source.parse_label()
         self.help = input_source.parse_help()
-        sanitizer_elem = input_source.parse_sanitizer_elem()
-        if sanitizer_elem is not None:
+        if (sanitizer_elem := input_source.parse_sanitizer_elem()) is not None:
             self.sanitizer = ToolParameterSanitizer.from_element(sanitizer_elem)
         else:
             self.sanitizer = None
@@ -1618,8 +1616,7 @@ def recurse_option_elems(cur_options, option_elems):
         self.display = elem.get("display", None)
         self.hierarchy = elem.get("hierarchy", "exact")  # exact or recurse
         self.separator = elem.get("separator", ",")
-        from_file = elem.get("from_file", None)
-        if from_file:
+        if from_file := elem.get("from_file", None):
             if not os.path.isabs(from_file):
                 from_file = os.path.join(tool.app.config.tool_data_path, from_file)
             elem = XML(f"<root>{open(from_file).read()}</root>")
@@ -1906,8 +1903,7 @@ def get_initial_value(self, trans, other_values):
             return RuntimeValue()
         if self.optional:
             return None
-        history = trans.history
-        if history is not None:
+        if (history := trans.history) is not None:
             dataset_matcher_factory = get_dataset_matcher_factory(trans)
             dataset_matcher = dataset_matcher_factory.dataset_matcher(self, other_values)
             if isinstance(self, DataToolParameter):
@@ -2651,8 +2647,7 @@ def __init__(self, tool, input_source, context=None):
     def to_dict(self, trans, other_values=None):
         other_values = other_values or {}
         d = ToolParameter.to_dict(self, trans)
-        target_name = self.data_ref
-        if target_name in other_values:
+        if (target_name := self.data_ref) in other_values:
             target = other_values[target_name]
             if not is_runtime_value(target):
                 d["target"] = {
diff --git a/lib/galaxy/tools/parameters/dataset_matcher.py b/lib/galaxy/tools/parameters/dataset_matcher.py
index c7fcf12fc759..e810abf538ec 100644
--- a/lib/galaxy/tools/parameters/dataset_matcher.py
+++ b/lib/galaxy/tools/parameters/dataset_matcher.py
@@ -246,8 +246,7 @@ def __valid_element(self, element):
         if element.ldda:
             return False
 
-        child_collection = element.child_collection
-        if child_collection:
+        if child_collection := element.child_collection:
             return self.dataset_collection_match(child_collection)
 
         hda = element.hda
diff --git a/lib/galaxy/tools/parameters/grouping.py b/lib/galaxy/tools/parameters/grouping.py
index 4f5795664455..ad18c4d55958 100644
--- a/lib/galaxy/tools/parameters/grouping.py
+++ b/lib/galaxy/tools/parameters/grouping.py
@@ -412,8 +412,7 @@ def get_file_name(file_name):
                 return Bunch(type=None, path=None, name=None)
 
         def get_url_paste_urls_or_filename(group_incoming, override_name=None, override_info=None):
-            url_paste_file = group_incoming.get("url_paste", None)
-            if url_paste_file is not None:
+            if (url_paste_file := group_incoming.get("url_paste", None)) is not None:
                 url_paste = open(url_paste_file).read()
 
                 def start_of_url(content):
diff --git a/lib/galaxy/tools/test.py b/lib/galaxy/tools/test.py
index ea84995943aa..41921dd4eaea 100644
--- a/lib/galaxy/tools/test.py
+++ b/lib/galaxy/tools/test.py
@@ -369,8 +369,7 @@ def __init__(self, name, index=None, parent_context=None):
 
     def for_state(self):
         name = self.name if self.index is None else "%s_%d" % (self.name, self.index)
-        parent_for_state = self.parent_context.for_state()
-        if parent_for_state:
+        if parent_for_state := self.parent_context.for_state():
             return f"{parent_for_state}|{name}"
         else:
             return name
diff --git a/lib/galaxy/tools/wrappers.py b/lib/galaxy/tools/wrappers.py
index 9e59536c6529..25e8ce9a9d4e 100644
--- a/lib/galaxy/tools/wrappers.py
+++ b/lib/galaxy/tools/wrappers.py
@@ -792,9 +792,8 @@ def __init__(self, input_datasets: Optional[Dict[str, Any]] = None) -> None:
             self.identifier_key_dict = {}
 
     def identifier(self, dataset_value: str, input_values: Dict[str, str]) -> Optional[str]:
-        identifier_key = self.identifier_key_dict.get(dataset_value, None)
         element_identifier = None
-        if identifier_key:
+        if identifier_key := self.identifier_key_dict.get(dataset_value, None):
             element_identifier = input_values.get(identifier_key, None)
 
         return element_identifier
diff --git a/lib/galaxy/util/__init__.py b/lib/galaxy/util/__init__.py
index 2db3e454bb22..af5c405d3609 100644
--- a/lib/galaxy/util/__init__.py
+++ b/lib/galaxy/util/__init__.py
@@ -57,7 +57,7 @@
     remap,
 )
 from requests.adapters import HTTPAdapter
-from requests.packages.urllib3.util.retry import Retry
+from requests.packages.urllib3.util.retry import Retry  # type: ignore[import-untyped]
 from typing_extensions import Literal
 
 try:
diff --git a/lib/galaxy/util/commands.py b/lib/galaxy/util/commands.py
index 321dd71f2fad..6238431c0e44 100644
--- a/lib/galaxy/util/commands.py
+++ b/lib/galaxy/util/commands.py
@@ -55,7 +55,7 @@ def redirect_aware_commmunicate(p, sys=_sys):
     return out, err
 
 
-def shell(cmds: Union[List[str], str], env: Optional[Dict[str, str]] = None, **kwds: Dict[str, Any]) -> int:
+def shell(cmds: Union[List[str], str], env: Optional[Dict[str, str]] = None, **kwds: Any) -> int:
     """Run shell commands with `shell_process` and wait."""
     sys = kwds.get("sys", _sys)
     assert sys is not None
@@ -68,9 +68,7 @@ def shell(cmds: Union[List[str], str], env: Optional[Dict[str, str]] = None, **k
         return p.wait()
 
 
-def shell_process(
-    cmds: Union[List[str], str], env: Optional[Dict[str, str]] = None, **kwds: Dict[str, Any]
-) -> subprocess.Popen:
+def shell_process(cmds: Union[List[str], str], env: Optional[Dict[str, str]] = None, **kwds: Any) -> subprocess.Popen:
     """A high-level method wrapping subprocess.Popen.
 
     Handles details such as environment extension and in process I/O
diff --git a/lib/galaxy/util/resources.py b/lib/galaxy/util/resources.py
index a16a78f7202b..071921a68b77 100644
--- a/lib/galaxy/util/resources.py
+++ b/lib/galaxy/util/resources.py
@@ -1,24 +1,38 @@
 """Provide a consistent interface into and utilities for importlib file resources.
 """
-try:
-    from importlib.abc import Traversable  # type: ignore[attr-defined]
-    from importlib.resources import files  # type: ignore[attr-defined]
-except ImportError:
-    # Python < 3.9
-    from importlib_resources import files  # type: ignore[no-redef]
-    from importlib_resources.abc import Traversable  # type: ignore[no-redef]
-
-
-def resource_path(package_or_requirement, resource_name):
+import sys
+
+if sys.version_info >= (3, 12):
+    from importlib.resources import (
+        Anchor,
+        as_file,
+        files,
+    )
+    from importlib.resources.abc import Traversable
+elif sys.version_info >= (3, 9):
+    from importlib.abc import Traversable
+    from importlib.resources import (
+        as_file,
+        files,
+        Package as Anchor,
+    )
+else:
+    from importlib_resources import (
+        as_file,
+        files,
+        Package as Anchor,
+    )
+    from importlib_resources.abc import Traversable
+
+
+def resource_path(package_or_requirement: Anchor, resource_name: str) -> Traversable:
     """
-    Return specified resource as a string.
-
-    Replacement function for pkg_resources.resource_string, but returns unicode string instead of bytestring.
+    Return specified resource as a Traversable.
     """
     return files(package_or_requirement).joinpath(resource_name)
 
 
-def resource_string(package_or_requirement, resource_name) -> str:
+def resource_string(package_or_requirement: Anchor, resource_name: str) -> str:
     """
     Return specified resource as a string.
 
@@ -28,6 +42,7 @@ def resource_string(package_or_requirement, resource_name) -> str:
 
 
 __all__ = (
+    "as_file",
     "files",
     "resource_string",
     "resource_path",
diff --git a/lib/galaxy/visualization/data_providers/genome.py b/lib/galaxy/visualization/data_providers/genome.py
index 7d01fd4f3558..0ef56f6b9810 100644
--- a/lib/galaxy/visualization/data_providers/genome.py
+++ b/lib/galaxy/visualization/data_providers/genome.py
@@ -1233,8 +1233,7 @@ def summarize_region(bbi, chrom, start, end, num_points):
             # Get summary; this samples at intervals of length
             # (end - start)/num_points -- i.e. drops any fractional component
             # of interval length.
-            summary = _summarize_bbi(bbi, chrom, start, end, num_points)
-            if summary:
+            if summary := _summarize_bbi(bbi, chrom, start, end, num_points):
                 # mean = summary.sum_data / summary.valid_count
 
                 # Standard deviation by bin, not yet used
diff --git a/lib/galaxy/visualization/plugins/config_parser.py b/lib/galaxy/visualization/plugins/config_parser.py
index c2867c13439b..85b1ccc79be9 100644
--- a/lib/galaxy/visualization/plugins/config_parser.py
+++ b/lib/galaxy/visualization/plugins/config_parser.py
@@ -148,18 +148,15 @@ def parse_visualization(self, xml_tree):
         # consider unifying the above into its own element and parsing method
 
         # load optional custom configuration specifiers
-        specs_section = xml_tree.find("specs")
-        if specs_section is not None:
+        if (specs_section := xml_tree.find("specs")) is not None:
             returned["specs"] = DictParser(specs_section)
 
         # load group specifiers
-        groups_section = xml_tree.find("groups")
-        if groups_section is not None:
+        if (groups_section := xml_tree.find("groups")) is not None:
             returned["groups"] = ListParser(groups_section)
 
         # load settings specifiers
-        settings_section = xml_tree.find("settings")
-        if settings_section is not None:
+        if (settings_section := xml_tree.find("settings")) is not None:
             returned["settings"] = ListParser(settings_section)
 
         return returned
@@ -226,8 +223,7 @@ def parse(self, xml_tree):
 
         # to_params (optional, 0 or more) - tells the registry to set certain params based on the model_clas, tests
         returned["to_params"] = {}
-        to_params = self.parse_to_params(xml_tree.findall("to_param"))
-        if to_params:
+        if to_params := self.parse_to_params(xml_tree.findall("to_param")):
             returned["to_params"] = to_params
 
         return returned
@@ -464,16 +460,14 @@ def parse(self, xml_tree):
         # NOTE: the interpretation of this list is deferred till parsing and based on param type
         #   e.g. it could be 'val in constrain_to', or 'constrain_to is min, max for number', etc.
         # TODO: currently unused
-        constrain_to = xml_tree.get("constrain_to")
-        if constrain_to:
+        if constrain_to := xml_tree.get("constrain_to"):
             returned["constrain_to"] = constrain_to.split(",")
 
         # is the param a comma-separated-value list?
         returned["csv"] = xml_tree.get("csv") == "true"
 
         # remap keys in the params/query string to the var names used in the template
-        var_name_in_template = xml_tree.get("var_name_in_template")
-        if var_name_in_template:
+        if var_name_in_template := xml_tree.get("var_name_in_template"):
             returned["var_name_in_template"] = var_name_in_template
 
         return returned
diff --git a/lib/galaxy/visualization/plugins/registry.py b/lib/galaxy/visualization/plugins/registry.py
index ff050ec0f504..41b0cf2eeaed 100644
--- a/lib/galaxy/visualization/plugins/registry.py
+++ b/lib/galaxy/visualization/plugins/registry.py
@@ -247,8 +247,7 @@ def get_visualization(self, trans, visualization_name, target_object):
         `visualization_name` if it's applicable to `target_object` or
         `None` if it's not.
         """
-        visualization = self.plugins.get(visualization_name, None)
-        if visualization is not None:
+        if (visualization := self.plugins.get(visualization_name, None)) is not None:
             data_sources = visualization.config["data_sources"]
             for data_source in data_sources:
                 model_class = data_source["model_class"]
diff --git a/lib/galaxy/web/framework/__init__.py b/lib/galaxy/web/framework/__init__.py
index a62442893e9b..f08ff3137507 100644
--- a/lib/galaxy/web/framework/__init__.py
+++ b/lib/galaxy/web/framework/__init__.py
@@ -26,9 +26,8 @@ def legacy_url_for(mapper, *args, **kwargs) -> str:
     Re-establishes the mapper for legacy WSGI routes.
     """
     rc = request_config()
-    environ = kwargs.pop("environ", None)
     rc.mapper = mapper
-    if environ:
+    if environ := kwargs.pop("environ", None):
         rc.environ = environ
         if hasattr(rc, "using_request_local"):
             rc.request_local = lambda: rc
diff --git a/lib/galaxy/web/framework/base.py b/lib/galaxy/web/framework/base.py
index baa1a07128e4..9c2b777257a7 100644
--- a/lib/galaxy/web/framework/base.py
+++ b/lib/galaxy/web/framework/base.py
@@ -426,8 +426,7 @@ def remote_hostname(self):
     @lazy_property
     def cookies(self):
         cookies = SimpleCookie()
-        cookie_header = self.environ.get("HTTP_COOKIE")
-        if cookie_header:
+        if cookie_header := self.environ.get("HTTP_COOKIE"):
             all_cookies = webob.cookies.parse_cookie(cookie_header)
             galaxy_cookies = {k.decode(): v.decode() for k, v in all_cookies if k.startswith(b"galaxy")}
             if galaxy_cookies:
diff --git a/lib/galaxy/web/framework/decorators.py b/lib/galaxy/web/framework/decorators.py
index 1db78d104542..1b00a59bf32d 100644
--- a/lib/galaxy/web/framework/decorators.py
+++ b/lib/galaxy/web/framework/decorators.py
@@ -236,8 +236,7 @@ def __extract_payload_from_request(trans, func, kwargs):
         # should ideally be in reverse, with the if clause being a check for application/json and the else clause assuming a standard encoding
         # such as multipart/form-data. Leaving it as is for backward compatibility, just in case.
         payload = loads(unicodify(trans.request.body))
-    run_as = trans.request.headers.get("run-as")
-    if run_as:
+    if run_as := trans.request.headers.get("run-as"):
         payload["run_as"] = run_as
     return payload
 
@@ -396,8 +395,7 @@ def validation_error_to_message_exception(e: ValidationError) -> MessageExceptio
 
 
 def api_error_message(trans, **kwds):
-    exception = kwds.get("exception", None)
-    if exception:
+    if exception := kwds.get("exception", None):
         # If we are passed a MessageException use err_msg.
         default_error_code = getattr(exception, "err_code", error_codes.UNKNOWN)
         default_error_message = getattr(exception, "err_msg", default_error_code.default_error_message)
diff --git a/lib/galaxy/web/framework/helpers/grids.py b/lib/galaxy/web/framework/helpers/grids.py
index 96e7114786ca..c4fd321c1d65 100644
--- a/lib/galaxy/web/framework/helpers/grids.py
+++ b/lib/galaxy/web/framework/helpers/grids.py
@@ -722,9 +722,8 @@ def __call__(self, trans, **kwargs):
         # Maintain sort state in generated urls
         extra_url_args = {}
         # Determine whether use_default_filter flag is set.
-        use_default_filter_str = kwargs.get("use_default_filter")
         use_default_filter = False
-        if use_default_filter_str:
+        if use_default_filter_str := kwargs.get("use_default_filter"):
             use_default_filter = use_default_filter_str.lower() == "true"
         # Process filtering arguments to (a) build a query that represents the filter and (b) build a
         # dictionary that denotes the current filter.
diff --git a/lib/galaxy/web/framework/middleware/static.py b/lib/galaxy/web/framework/middleware/static.py
index 1ac00814c577..d46ff6af592c 100644
--- a/lib/galaxy/web/framework/middleware/static.py
+++ b/lib/galaxy/web/framework/middleware/static.py
@@ -55,8 +55,7 @@ def __call__(self, environ, start_response):
             return self.__class__(full)(environ, start_response)
         if environ.get("PATH_INFO") and environ.get("PATH_INFO") != "/":
             return self.error_extra_path(environ, start_response)
-        if_none_match = environ.get("HTTP_IF_NONE_MATCH")
-        if if_none_match:
+        if if_none_match := environ.get("HTTP_IF_NONE_MATCH"):
             mytime = os.stat(full).st_mtime
             if str(mytime) == if_none_match:
                 headers: List[Tuple[str, str]] = []
diff --git a/lib/galaxy/web/framework/middleware/xforwardedhost.py b/lib/galaxy/web/framework/middleware/xforwardedhost.py
index 3e0e11958632..3376fd03c953 100644
--- a/lib/galaxy/web/framework/middleware/xforwardedhost.py
+++ b/lib/galaxy/web/framework/middleware/xforwardedhost.py
@@ -8,19 +8,16 @@ def __init__(self, app, global_conf=None):
         self.app = app
 
     def __call__(self, environ, start_response):
-        x_forwarded_host = environ.get("HTTP_X_FORWARDED_HOST", None)
-        if x_forwarded_host:
+        if x_forwarded_host := environ.get("HTTP_X_FORWARDED_HOST", None):
             environ["ORGINAL_HTTP_HOST"] = environ["HTTP_HOST"]
             environ["HTTP_HOST"] = x_forwarded_host.split(", ", 1)[0]
-        x_forwarded_for = environ.get("HTTP_X_FORWARDED_FOR", None)
-        if x_forwarded_for:
+        if x_forwarded_for := environ.get("HTTP_X_FORWARDED_FOR", None):
             environ["ORGINAL_REMOTE_ADDR"] = environ["REMOTE_ADDR"]
             environ["REMOTE_ADDR"] = x_forwarded_for.split(",", 1)[0].strip()
         x_forwarded_proto = environ.get("HTTP_X_FORWARDED_PROTO", None)
         if x_forwarded_proto:
             x_forwarded_proto = x_forwarded_proto.split(",", 1)[0].strip()
-        x_url_scheme = environ.get("HTTP_X_URL_SCHEME", x_forwarded_proto)
-        if x_url_scheme:
+        if x_url_scheme := environ.get("HTTP_X_URL_SCHEME", x_forwarded_proto):
             environ["original_wsgi.url_scheme"] = environ["wsgi.url_scheme"]
             environ["wsgi.url_scheme"] = x_url_scheme
         return self.app(environ, start_response)
diff --git a/lib/galaxy/web/legacy_framework/grids.py b/lib/galaxy/web/legacy_framework/grids.py
index 8b4fca1f8c5f..3b62e8665012 100644
--- a/lib/galaxy/web/legacy_framework/grids.py
+++ b/lib/galaxy/web/legacy_framework/grids.py
@@ -682,9 +682,8 @@ def __call__(self, trans, **kwargs):
         # Maintain sort state in generated urls
         extra_url_args = {}
         # Determine whether use_default_filter flag is set.
-        use_default_filter_str = kwargs.get("use_default_filter")
         use_default_filter = False
-        if use_default_filter_str:
+        if use_default_filter_str := kwargs.get("use_default_filter"):
             use_default_filter = use_default_filter_str.lower() == "true"
         # Process filtering arguments to (a) build a query that represents the filter and (b) build a
         # dictionary that denotes the current filter.
diff --git a/lib/galaxy/web/statsd_client.py b/lib/galaxy/web/statsd_client.py
index 99ebbabb091f..cf83b299bc52 100644
--- a/lib/galaxy/web/statsd_client.py
+++ b/lib/galaxy/web/statsd_client.py
@@ -54,8 +54,7 @@ def _effective_infix(self, path, tags):
 
 class PyTestGalaxyStatsdClient(VanillaGalaxyStatsdClient):
     def timing(self, path, time, tags=None):
-        metrics = CURRENT_TEST_METRICS
-        if metrics is not None:
+        if (metrics := CURRENT_TEST_METRICS) is not None:
             timing = metrics["timing"]
             if path not in timing:
                 timing[path] = []
@@ -63,8 +62,7 @@ def timing(self, path, time, tags=None):
         super().timing(path, time, tags=tags)
 
     def incr(self, path, n=1, tags=None):
-        metrics = CURRENT_TEST_METRICS
-        if metrics is not None:
+        if (metrics := CURRENT_TEST_METRICS) is not None:
             counter = metrics["counter"]
             if path not in counter:
                 counter[path] = []
@@ -72,8 +70,7 @@ def incr(self, path, n=1, tags=None):
         super().incr(path, n=n, tags=tags)
 
     def _effective_infix(self, path, tags):
-        current_test = CURRENT_TEST
-        if current_test is not None:
+        if (current_test := CURRENT_TEST) is not None:
             tags = tags or {}
             tags["test"] = current_test
         return super()._effective_infix(path, tags)
diff --git a/lib/galaxy/webapps/base/api.py b/lib/galaxy/webapps/base/api.py
index 73056bc88a09..a1804255f228 100644
--- a/lib/galaxy/webapps/base/api.py
+++ b/lib/galaxy/webapps/base/api.py
@@ -161,8 +161,7 @@ def add_sentry_middleware(app: FastAPI) -> None:
 def get_error_response_for_request(request: Request, exc: MessageException) -> JSONResponse:
     error_dict = api_error_message(None, exception=exc)
     status_code = exc.status_code
-    path = request.url.path
-    if "ga4gh" in path:
+    if "ga4gh" in (path := request.url.path):
         # When serving GA4GH APIs use limited exceptions to conform their expected
         # error schema. Tailored to DRS currently.
         message = error_dict["err_msg"]
diff --git a/lib/galaxy/webapps/base/controller.py b/lib/galaxy/webapps/base/controller.py
index 3a2eb11ac13f..6c0f41d96978 100644
--- a/lib/galaxy/webapps/base/controller.py
+++ b/lib/galaxy/webapps/base/controller.py
@@ -225,8 +225,7 @@ def _parse_serialization_params(self, kwd, default_view):
 
     # TODO: this will be replaced by lib.galaxy.schema.FilterQueryParams.build_order_by
     def _parse_order_by(self, manager, order_by_string):
-        ORDER_BY_SEP_CHAR = ","
-        if ORDER_BY_SEP_CHAR in order_by_string:
+        if (ORDER_BY_SEP_CHAR := ",") in order_by_string:
             return [manager.parse_order_by(o) for o in order_by_string.split(ORDER_BY_SEP_CHAR)]
         return manager.parse_order_by(order_by_string)
 
@@ -1069,8 +1068,7 @@ def _get_genome_data(self, trans, dataset, dbkey=None):
 
         # If there are no messages (messages indicate data is not ready/available), get data.
         messages_list = [data_source_dict["message"] for data_source_dict in data_sources.values()]
-        message = self._get_highest_priority_msg(messages_list)
-        if message:
+        if message := self._get_highest_priority_msg(messages_list):
             rval = message
         else:
             # HACK: chromatin interactions tracks use data as source.
diff --git a/lib/galaxy/webapps/base/webapp.py b/lib/galaxy/webapps/base/webapp.py
index 8fc17986afec..97bd22c768d1 100644
--- a/lib/galaxy/webapps/base/webapp.py
+++ b/lib/galaxy/webapps/base/webapp.py
@@ -309,8 +309,7 @@ def __init__(
         super().__init__(environ)
         config = self.app.config
         self.debug = asbool(config.get("debug", False))
-        x_frame_options = getattr(config, "x_frame_options", None)
-        if x_frame_options:
+        if x_frame_options := getattr(config, "x_frame_options", None):
             self.response.headers["X-Frame-Options"] = x_frame_options
         # Flag indicating whether we are in workflow building mode (means
         # that the current history should not be used for parameter values
diff --git a/lib/galaxy/webapps/galaxy/api/__init__.py b/lib/galaxy/webapps/galaxy/api/__init__.py
index b6a44a1f43ac..4c8df89bee77 100644
--- a/lib/galaxy/webapps/galaxy/api/__init__.py
+++ b/lib/galaxy/webapps/galaxy/api/__init__.py
@@ -214,9 +214,8 @@ def base(self) -> str:
     @property
     def url_path(self) -> str:
         scope = self.__request.scope
-        root_path = scope.get("root_path")
         url = self.base
-        if root_path:
+        if root_path := scope.get("root_path"):
             url = urljoin(url, root_path)
         return url
 
@@ -558,8 +557,7 @@ class IndexQueryTag(NamedTuple):
 
     def as_markdown(self):
         desc = self.description
-        alias = self.alias
-        if alias:
+        if alias := self.alias:
             desc += f" (The tag `{alias}` can be used a short hand alias for this tag to filter on this attribute.)"
         if self.admin_only:
             desc += " This tag is only available for requests using admin keys and/or sessions."
diff --git a/lib/galaxy/webapps/galaxy/api/annotations.py b/lib/galaxy/webapps/galaxy/api/annotations.py
index fc56b54a45a3..f11fea9660a1 100644
--- a/lib/galaxy/webapps/galaxy/api/annotations.py
+++ b/lib/galaxy/webapps/galaxy/api/annotations.py
@@ -28,8 +28,7 @@ class BaseAnnotationsController(BaseGalaxyAPIController, UsesStoredWorkflowMixin
     @expose_api
     def index(self, trans: ProvidesHistoryContext, **kwd):
         idnum = kwd[self.tagged_item_id]
-        item = self._get_item_from_id(trans, idnum)
-        if item is not None:
+        if (item := self._get_item_from_id(trans, idnum)) is not None:
             return self.get_item_annotation_str(trans.sa_session, trans.user, item)
 
     @expose_api
@@ -37,8 +36,7 @@ def create(self, trans: ProvidesHistoryContext, payload: dict, **kwd):
         if "text" not in payload:
             return ""
         idnum = kwd[self.tagged_item_id]
-        item = self._get_item_from_id(trans, idnum)
-        if item is not None:
+        if (item := self._get_item_from_id(trans, idnum)) is not None:
             new_annotation = payload.get("text")
             # TODO: sanitize on display not entry
             new_annotation = sanitize_html(new_annotation)
@@ -52,8 +50,7 @@ def create(self, trans: ProvidesHistoryContext, payload: dict, **kwd):
     @expose_api
     def delete(self, trans: ProvidesHistoryContext, **kwd):
         idnum = kwd[self.tagged_item_id]
-        item = self._get_item_from_id(trans, idnum)
-        if item is not None:
+        if (item := self._get_item_from_id(trans, idnum)) is not None:
             return self.delete_item_annotation(trans.sa_session, trans.user, item)
 
     @expose_api
diff --git a/lib/galaxy/webapps/galaxy/api/drs.py b/lib/galaxy/webapps/galaxy/api/drs.py
index b5960ee49f4f..d0efa39c62e8 100644
--- a/lib/galaxy/webapps/galaxy/api/drs.py
+++ b/lib/galaxy/webapps/galaxy/api/drs.py
@@ -63,9 +63,8 @@ def service_info(self, request: Request) -> Service:
             artifact="drs",
             version="1.2.0",
         )
-        environment = config.ga4gh_service_environment
         extra_kwds = {}
-        if environment:
+        if environment := config.ga4gh_service_environment:
             extra_kwds["environment"] = environment
         return Service(
             id=organization_id + ".drs",
diff --git a/lib/galaxy/webapps/galaxy/api/library_contents.py b/lib/galaxy/webapps/galaxy/api/library_contents.py
index fb8eddd6eff1..7f791ef6be2a 100644
--- a/lib/galaxy/webapps/galaxy/api/library_contents.py
+++ b/lib/galaxy/webapps/galaxy/api/library_contents.py
@@ -332,7 +332,6 @@ def _upload_library_dataset(self, trans, folder_id: int, **kwd):
             last_used_build = dbkey[0]
         else:
             last_used_build = dbkey
-        roles = kwd.get("roles", "")
         is_admin = trans.user_is_admin
         current_user_roles = trans.get_current_user_roles()
         folder = trans.sa_session.get(LibraryFolder, folder_id)
@@ -344,7 +343,7 @@ def _upload_library_dataset(self, trans, folder_id: int, **kwd):
         error = False
         if upload_option == "upload_paths":
             validate_path_upload(trans)  # Duplicate check made in _upload_dataset.
-        elif roles:
+        elif roles := kwd.get("roles", ""):
             # Check to see if the user selected roles to associate with the DATASET_ACCESS permission
             # on the dataset that would cause accessibility issues.
             vars = dict(DATASET_ACCESS_in=roles)
diff --git a/lib/galaxy/webapps/galaxy/api/library_datasets.py b/lib/galaxy/webapps/galaxy/api/library_datasets.py
index 2ce680ad6812..7f34ff0ab840 100644
--- a/lib/galaxy/webapps/galaxy/api/library_datasets.py
+++ b/lib/galaxy/webapps/galaxy/api/library_datasets.py
@@ -434,8 +434,7 @@ def load(self, trans, payload=None, **kwd):
         kwd["file_type"] = kwd.get("file_type", "auto")
         kwd["link_data_only"] = "link_to_files" if util.string_as_bool(kwd.get("link_data", False)) else "copy_files"
         kwd["tag_using_filenames"] = util.string_as_bool(kwd.get("tag_using_filenames", None))
-        encoded_folder_id = kwd.get("encoded_folder_id", None)
-        if encoded_folder_id is not None:
+        if (encoded_folder_id := kwd.get("encoded_folder_id", None)) is not None:
             folder_id = self.folder_manager.cut_and_decode(trans, encoded_folder_id)
         else:
             raise exceptions.RequestParameterMissingException("The required attribute encoded_folder_id is missing.")
diff --git a/lib/galaxy/webapps/galaxy/api/plugins.py b/lib/galaxy/webapps/galaxy/api/plugins.py
index 68f6a3c1d931..1a1c88fd4f20 100644
--- a/lib/galaxy/webapps/galaxy/api/plugins.py
+++ b/lib/galaxy/webapps/galaxy/api/plugins.py
@@ -35,8 +35,7 @@ def index(self, trans, **kwargs):
         GET /api/plugins:
         """
         registry = self._get_registry()
-        dataset_id = kwargs.get("dataset_id")
-        if dataset_id is not None:
+        if (dataset_id := kwargs.get("dataset_id")) is not None:
             hda = self.hda_manager.get_accessible(self.decode_id(dataset_id), trans.user)
             return registry.get_visualizations(trans, hda)
         else:
@@ -49,8 +48,7 @@ def show(self, trans, id, **kwargs):
         GET /api/plugins/{id}:
         """
         registry = self._get_registry()
-        history_id = kwargs.get("history_id")
-        if history_id is not None:
+        if (history_id := kwargs.get("history_id")) is not None:
             history = self.history_manager.get_owned(
                 trans.security.decode_id(history_id), trans.user, current_history=trans.history
             )
diff --git a/lib/galaxy/webapps/galaxy/api/tool_shed_repositories.py b/lib/galaxy/webapps/galaxy/api/tool_shed_repositories.py
index 471eb1d3b6b6..88ade5498482 100644
--- a/lib/galaxy/webapps/galaxy/api/tool_shed_repositories.py
+++ b/lib/galaxy/webapps/galaxy/api/tool_shed_repositories.py
@@ -318,8 +318,7 @@ def __parse_repository_from_payload(self, payload, include_changeset=False):
     @require_admin
     @expose_api
     def reset_metadata_on_selected_installed_repositories(self, trans, **kwd):
-        repository_ids = util.listify(kwd.get("repository_ids"))
-        if repository_ids:
+        if repository_ids := util.listify(kwd.get("repository_ids")):
             irmm = InstalledRepositoryMetadataManager(self.app)
             failed = []
             successful = []
diff --git a/lib/galaxy/webapps/galaxy/api/tools.py b/lib/galaxy/webapps/galaxy/api/tools.py
index 7e9d0b133b8c..38f346bffe8e 100644
--- a/lib/galaxy/webapps/galaxy/api/tools.py
+++ b/lib/galaxy/webapps/galaxy/api/tools.py
@@ -238,9 +238,8 @@ def build(self, trans: GalaxyWebTransaction, id, **kwd):
         """
         kwd = _kwd_or_payload(kwd)
         tool_version = kwd.get("tool_version")
-        history_id = kwd.pop("history_id", None)
         history = None
-        if history_id:
+        if history_id := kwd.pop("history_id", None):
             history = self.history_manager.get_owned(
                 self.decode_id(history_id), trans.user, current_history=trans.history
             )
@@ -275,8 +274,7 @@ def test_data_download(self, trans: GalaxyWebTransaction, id, **kwd):
         filename = kwd.get("filename")
         if filename is None:
             raise exceptions.ObjectNotFound("Test data filename not specified.")
-        path = tool.test_data_path(filename)
-        if path:
+        if path := tool.test_data_path(filename):
             if os.path.isfile(path):
                 trans.response.headers["Content-Disposition"] = f'attachment; filename="{filename}"'
                 return open(path, mode="rb")
@@ -463,9 +461,8 @@ def to_dict(x):
             lineage_dict = tool.lineage.to_dict()
         else:
             lineage_dict = None
-        tool_shed_dependencies = tool.installed_tool_dependencies
         tool_shed_dependencies_dict: Optional[list] = None
-        if tool_shed_dependencies:
+        if tool_shed_dependencies := tool.installed_tool_dependencies:
             tool_shed_dependencies_dict = list(map(to_dict, tool_shed_dependencies))
         return {
             "tool_id": tool.id,
@@ -516,8 +513,7 @@ def conversion(self, trans: GalaxyWebTransaction, tool_id, payload, **kwd):
             ],
             "batch": input_src == "hdca",
         }
-        history_id = payload.get("history_id")
-        if history_id:
+        if history_id := payload.get("history_id"):
             decoded_id = self.decode_id(history_id)
             target_history = self.history_manager.get_owned(decoded_id, trans.user, current_history=trans.history)
         else:
diff --git a/lib/galaxy/webapps/galaxy/api/users.py b/lib/galaxy/webapps/galaxy/api/users.py
index 3deac727ac6d..1ab007693020 100644
--- a/lib/galaxy/webapps/galaxy/api/users.py
+++ b/lib/galaxy/webapps/galaxy/api/users.py
@@ -276,8 +276,7 @@ def usage(
         trans: ProvidesUserContext = DependsOnTrans,
         user_id: FlexibleUserIdType = FlexibleUserIdPathParam,
     ) -> List[UserQuotaUsage]:
-        user = self.service.get_user_full(trans, user_id, False)
-        if user:
+        if user := self.service.get_user_full(trans, user_id, False):
             rval = self.user_serializer.serialize_disk_usage(user)
             return rval
         else:
@@ -294,11 +293,10 @@ def usage_for(
         user_id: FlexibleUserIdType = FlexibleUserIdPathParam,
         label: str = QuotaSourceLabelPathParam,
     ) -> Optional[UserQuotaUsage]:
-        user = self.service.get_user_full(trans, user_id, False)
         effective_label: Optional[str] = label
         if label == "__null__":
             effective_label = None
-        if user:
+        if user := self.service.get_user_full(trans, user_id, False):
             rval = self.user_serializer.serialize_disk_usage_for(user, effective_label)
             return rval
         else:
@@ -907,8 +905,7 @@ def set_information(self, trans, id, payload=None, **kwd):
             if user.username != username:
                 user.username = username
         # Update user custom form
-        user_info_form_id = payload.get("info|form_id")
-        if user_info_form_id:
+        if user_info_form_id := payload.get("info|form_id"):
             prefix = "info|"
             user_info_form = trans.sa_session.get(FormDefinition, trans.security.decode_id(user_info_form_id))
             user_info_values = {}
diff --git a/lib/galaxy/webapps/galaxy/api/workflows.py b/lib/galaxy/webapps/galaxy/api/workflows.py
index e362befa9608..086ddc2f40af 100644
--- a/lib/galaxy/webapps/galaxy/api/workflows.py
+++ b/lib/galaxy/webapps/galaxy/api/workflows.py
@@ -372,9 +372,8 @@ def workflow_dict(self, trans: GalaxyWebTransaction, workflow_id, **kwd):
         style = kwd.get("style", "export")
         download_format = kwd.get("format")
         version = kwd.get("version")
-        history_id = kwd.get("history_id")
         history = None
-        if history_id:
+        if history_id := kwd.get("history_id"):
             history = self.history_manager.get_accessible(
                 self.decode_id(history_id), trans.user, current_history=trans.history
             )
diff --git a/lib/galaxy/webapps/galaxy/buildapp.py b/lib/galaxy/webapps/galaxy/buildapp.py
index d573a83b1233..06ef4f455a62 100644
--- a/lib/galaxy/webapps/galaxy/buildapp.py
+++ b/lib/galaxy/webapps/galaxy/buildapp.py
@@ -1084,8 +1084,7 @@ def wrap_in_middleware(app, global_conf, application_stack, **local_conf):
     # other middleware):
     app = wrap_if_allowed(app, stack, httpexceptions.make_middleware, name="paste.httpexceptions", args=(conf,))
     # Statsd request timing and profiling
-    statsd_host = conf.get("statsd_host", None)
-    if statsd_host:
+    if statsd_host := conf.get("statsd_host", None):
         from galaxy.web.framework.middleware.statsd import StatsdMiddleware
 
         app = wrap_if_allowed(
diff --git a/lib/galaxy/webapps/galaxy/controllers/admin.py b/lib/galaxy/webapps/galaxy/controllers/admin.py
index 77142ca9669b..5495f24943fb 100644
--- a/lib/galaxy/webapps/galaxy/controllers/admin.py
+++ b/lib/galaxy/webapps/galaxy/controllers/admin.py
@@ -516,8 +516,7 @@ class ToolVersionsColumn(grids.TextColumn):
         def get_value(self, trans, grid, tool_version):
             tool_ids_str = ""
             toolbox = trans.app.toolbox
-            tool = toolbox._tools_by_id.get(tool_version.tool_id)
-            if tool:
+            if tool := toolbox._tools_by_id.get(tool_version.tool_id):
                 for tool_id in tool.lineage.tool_ids:
                     if toolbox.has_tool(tool_id, exact=True):
                         link = url_for(controller="tool_runner", tool_id=tool_id)
@@ -910,8 +909,7 @@ def impersonate(self, trans, **kwd):
         if not trans.app.config.allow_user_impersonation:
             return trans.show_error_message("User impersonation is not enabled in this instance of Galaxy.")
         user = None
-        user_id = kwd.get("id", None)
-        if user_id is not None:
+        if (user_id := kwd.get("id", None)) is not None:
             try:
                 user = trans.sa_session.query(trans.app.model.User).get(trans.security.decode_id(user_id))
                 if user:
diff --git a/lib/galaxy/webapps/galaxy/controllers/authnz.py b/lib/galaxy/webapps/galaxy/controllers/authnz.py
index 88af61d8fa11..2f6cde7d1ebe 100644
--- a/lib/galaxy/webapps/galaxy/controllers/authnz.py
+++ b/lib/galaxy/webapps/galaxy/controllers/authnz.py
@@ -202,13 +202,12 @@ def get_logout_url(self, trans, provider=None, **kwargs):
     @web.expose
     @web.json
     def get_cilogon_idps(self, trans, **kwargs):
-        allowed_idps = trans.app.authnz_manager.get_allowed_idps()
         try:
             cilogon_idps = json.loads(url_get("https://cilogon.org/idplist/", params=dict(kwargs)))
         except Exception as e:
             raise Exception(f"Invalid server response. {str(e)}.")
 
-        if allowed_idps:
+        if allowed_idps := trans.app.authnz_manager.get_allowed_idps():
             validated_idps = list(filter(lambda idp: idp["EntityID"] in allowed_idps, cilogon_idps))
 
             if not (len(validated_idps) == len(allowed_idps)):
diff --git a/lib/galaxy/webapps/galaxy/controllers/dataset.py b/lib/galaxy/webapps/galaxy/controllers/dataset.py
index df9f1f2f9e93..ebebec96a3e1 100644
--- a/lib/galaxy/webapps/galaxy/controllers/dataset.py
+++ b/lib/galaxy/webapps/galaxy/controllers/dataset.py
@@ -137,8 +137,7 @@ def display(
             return data
         if "hdca" in kwd:
             raise RequestParameterInvalidException("Invalid request parameter 'hdca' encountered.")
-        hdca_id = kwd.get("hdca_id", None)
-        if hdca_id:
+        if hdca_id := kwd.get("hdca_id", None):
             hdca = self.app.dataset_collection_manager.get_dataset_collection_instance(trans, "history", hdca_id)
             del kwd["hdca_id"]
             kwd["hdca"] = hdca
diff --git a/lib/galaxy/webapps/galaxy/controllers/user.py b/lib/galaxy/webapps/galaxy/controllers/user.py
index 52587ee2d66e..e661f3c40afe 100644
--- a/lib/galaxy/webapps/galaxy/controllers/user.py
+++ b/lib/galaxy/webapps/galaxy/controllers/user.py
@@ -248,8 +248,7 @@ def is_outside_grace_period(self, trans, create_time):
     @web.expose
     @web.json
     def logout(self, trans, logout_all=False, **kwd):
-        message = trans.check_csrf_token(kwd)
-        if message:
+        if message := trans.check_csrf_token(kwd):
             return self.message_exception(trans, message)
         # Since logging an event requires a session, we'll log prior to ending the session
         trans.log_event("User logged out")
@@ -341,8 +340,7 @@ def change_password(self, trans, payload=None, **kwd):
     def reset_password(self, trans, payload=None, **kwd):
         """Reset the user's password. Send an email with token that allows a password change."""
         payload = payload or {}
-        message = self.user_manager.send_reset_email(trans, payload)
-        if message:
+        if message := self.user_manager.send_reset_email(trans, payload):
             return self.message_exception(trans, message)
         return {"message": "Reset link has been sent to your email."}
 
diff --git a/lib/galaxy/webapps/galaxy/fast_app.py b/lib/galaxy/webapps/galaxy/fast_app.py
index 464f57270b09..792a0a5f56d0 100644
--- a/lib/galaxy/webapps/galaxy/fast_app.py
+++ b/lib/galaxy/webapps/galaxy/fast_app.py
@@ -99,8 +99,7 @@ def is_allowed_origin(self, origin: str) -> bool:
 
 
 def add_galaxy_middleware(app: FastAPI, gx_app):
-    x_frame_options = gx_app.config.x_frame_options
-    if x_frame_options:
+    if x_frame_options := gx_app.config.x_frame_options:
 
         @app.middleware("http")
         async def add_x_frame_options(request: Request, call_next):
diff --git a/lib/galaxy/webapps/galaxy/services/datasets.py b/lib/galaxy/webapps/galaxy/services/datasets.py
index 37741b0cdc49..4a40e2e2576a 100644
--- a/lib/galaxy/webapps/galaxy/services/datasets.py
+++ b/lib/galaxy/webapps/galaxy/services/datasets.py
@@ -850,15 +850,13 @@ def _data(
             return dataset.conversion_messages.NO_DATA
 
         # Dataset check.
-        msg = self.hda_manager.data_conversion_status(dataset)
-        if msg:
+        if msg := self.hda_manager.data_conversion_status(dataset):
             return msg
 
         # Get datasources and check for messages.
         data_sources = dataset.get_datasources(trans)
         messages_list = [data_source_dict["message"] for data_source_dict in data_sources.values()]
-        return_message = self._get_highest_priority_msg(messages_list)
-        if return_message:
+        if return_message := self._get_highest_priority_msg(messages_list):
             return return_message
 
         extra_info = None
@@ -953,8 +951,7 @@ def _raw_data(
         be slow because indexes need to be created.
         """
         # Dataset check.
-        msg = self.hda_manager.data_conversion_status(dataset)
-        if msg:
+        if msg := self.hda_manager.data_conversion_status(dataset):
             return msg
 
         registry = self.data_provider_registry
diff --git a/lib/galaxy/webapps/galaxy/services/history_contents.py b/lib/galaxy/webapps/galaxy/services/history_contents.py
index be4727ae0f52..a6d2a3becf2e 100644
--- a/lib/galaxy/webapps/galaxy/services/history_contents.py
+++ b/lib/galaxy/webapps/galaxy/services/history_contents.py
@@ -885,8 +885,7 @@ def __update_dataset(
     ):
         # anon user: ensure that history ids match up and the history is the current,
         #   check for uploading, and use only the subset of attribute keys manipulatable by anon users
-        hda = self.__datasets_for_update(trans, history, [id], payload)[0]
-        if hda:
+        if hda := self.__datasets_for_update(trans, history, [id], payload)[0]:
             self.__deserialize_dataset(trans, hda, payload)
             serialization_params.default_view = "detailed"
             return self.hda_serializer.serialize_to_view(
@@ -933,13 +932,11 @@ def __index_legacy(
         """Legacy implementation of the `index` action."""
         history = self._get_history(trans, history_id)
         legacy_params_dict = legacy_params.dict(exclude_defaults=True)
-        ids = legacy_params_dict.get("ids")
-        if ids:
+        if ids := legacy_params_dict.get("ids"):
             legacy_params_dict["ids"] = self.decode_ids(ids)
 
         object_store_ids = None
-        shareable = legacy_params.shareable
-        if shareable is not None:
+        if (shareable := legacy_params.shareable) is not None:
             object_store_ids = self.object_store.object_store_ids(private=not shareable)
             if object_store_ids:
                 legacy_params_dict["object_store_ids"] = object_store_ids
diff --git a/lib/galaxy/webapps/galaxy/services/jobs.py b/lib/galaxy/webapps/galaxy/services/jobs.py
index 95ceb3ede2b3..ec9e42467482 100644
--- a/lib/galaxy/webapps/galaxy/services/jobs.py
+++ b/lib/galaxy/webapps/galaxy/services/jobs.py
@@ -130,8 +130,7 @@ def dictify_associations(self, trans, *association_lists) -> List[JobAssociation
 
     def __dictify_association(self, trans, job_dataset_association) -> JobAssociation:
         dataset_dict = None
-        dataset = job_dataset_association.dataset
-        if dataset:
+        if dataset := job_dataset_association.dataset:
             if isinstance(dataset, model.HistoryDatasetAssociation):
                 dataset_dict = EncodedDatasetSourceId(src="hda", id=dataset.id)
             else:
diff --git a/lib/galaxy/webapps/galaxy/services/notifications.py b/lib/galaxy/webapps/galaxy/services/notifications.py
index 6ca78fafb568..12c86a218046 100644
--- a/lib/galaxy/webapps/galaxy/services/notifications.py
+++ b/lib/galaxy/webapps/galaxy/services/notifications.py
@@ -134,7 +134,7 @@ def update_user_notification(
     ):
         """Updates a single notification received by the user with the requested values."""
         self.notification_manager.ensure_notifications_enabled()
-        updated_response = self.update_user_notifications(user_context, set([notification_id]), request)
+        updated_response = self.update_user_notifications(user_context, {notification_id}, request)
         if not updated_response.updated_count:
             self._raise_notification_not_found(notification_id)
 
diff --git a/lib/galaxy/webapps/galaxy/services/tools.py b/lib/galaxy/webapps/galaxy/services/tools.py
index 9265f51427f5..8bdb7eb0d5a5 100644
--- a/lib/galaxy/webapps/galaxy/services/tools.py
+++ b/lib/galaxy/webapps/galaxy/services/tools.py
@@ -134,8 +134,7 @@ def _create(self, trans: ProvidesHistoryContext, payload, **kwd):
         # Set running history from payload parameters.
         # History not set correctly as part of this API call for
         # dataset upload.
-        history_id = payload.get("history_id")
-        if history_id:
+        if history_id := payload.get("history_id"):
             history_id = trans.security.decode_id(history_id) if isinstance(history_id, str) else history_id
             target_history = self.history_manager.get_mutable(history_id, trans.user, current_history=trans.history)
         else:
@@ -201,8 +200,7 @@ def _handle_inputs_output_to_api_response(self, trans, tool, target_history, var
         output_datasets = vars.get("out_data", [])
         rval: Dict[str, Any] = {"outputs": [], "output_collections": [], "jobs": [], "implicit_collections": []}
         rval["produces_entry_points"] = tool.produces_entry_points
-        job_errors = vars.get("job_errors", [])
-        if job_errors:
+        if job_errors := vars.get("job_errors", []):
             # If we are here - some jobs were successfully executed but some failed.
             rval["errors"] = job_errors
 
diff --git a/lib/galaxy/webapps/openapi/utils.py b/lib/galaxy/webapps/openapi/utils.py
index 5fa24c4597f8..6e6c79248f96 100644
--- a/lib/galaxy/webapps/openapi/utils.py
+++ b/lib/galaxy/webapps/openapi/utils.py
@@ -150,9 +150,8 @@ def get_openapi_operation_request_body(
     body_schema, _, _ = field_schema(body_field, model_name_map=model_name_map, ref_prefix=REF_PREFIX)
     field_info = cast(Body, body_field.field_info)
     request_media_type = field_info.media_type
-    required = body_field.required
     request_body_oai: Dict[str, Any] = {}
-    if required:
+    if required := body_field.required:
         request_body_oai["required"] = required
     request_media_content: Dict[str, Any] = {"schema": body_schema}
     if field_info.examples:
diff --git a/lib/galaxy/webapps/reports/controllers/history.py b/lib/galaxy/webapps/reports/controllers/history.py
index 4e36891fc0bb..cb6f184b55ab 100644
--- a/lib/galaxy/webapps/reports/controllers/history.py
+++ b/lib/galaxy/webapps/reports/controllers/history.py
@@ -159,7 +159,6 @@ def history_and_dataset_type(self, trans, **kwd):
         user_cutoff = int(kwd.get("user_cutoff", 60))
         descending = 1 if kwd.get("descending", "desc") == "desc" else -1
         reverse = descending == 1
-        user_selection = kwd.get("user_selection", None)
 
         # select d.state, h.name
         # from dataset d, history h , history_dataset_association hda
@@ -169,7 +168,7 @@ def history_and_dataset_type(self, trans, **kwd):
             galaxy.model.History.table,
             galaxy.model.HistoryDatasetAssociation.table,
         ]
-        if user_selection is not None:
+        if (user_selection := kwd.get("user_selection", None)) is not None:
             from_obj.append(galaxy.model.User.table)
             whereclause = and_(
                 galaxy.model.Dataset.table.c.id == galaxy.model.HistoryDatasetAssociation.table.c.dataset_id,
diff --git a/lib/galaxy/webapps/reports/controllers/jobs.py b/lib/galaxy/webapps/reports/controllers/jobs.py
index e281f538b868..3fbad58ba29f 100644
--- a/lib/galaxy/webapps/reports/controllers/jobs.py
+++ b/lib/galaxy/webapps/reports/controllers/jobs.py
@@ -1306,7 +1306,6 @@ def get_monitor_id(trans, monitor_email):
     A convenience method to obtain the monitor job id.
     """
     monitor_user_id = None
-    monitor_row = get_user_by_email(trans.sa_session, monitor_email)
-    if monitor_row is not None:
+    if (monitor_row := get_user_by_email(trans.sa_session, monitor_email)) is not None:
         monitor_user_id = monitor_row[0]
     return monitor_user_id
diff --git a/lib/galaxy/workflow/extract.py b/lib/galaxy/workflow/extract.py
index d349cb87bb26..199c096e1133 100644
--- a/lib/galaxy/workflow/extract.py
+++ b/lib/galaxy/workflow/extract.py
@@ -296,8 +296,7 @@ def __summarize_dataset_collection(self, dataset_collection):
 
         hid = dataset_collection.hid
         self.collection_types[hid] = dataset_collection.collection.collection_type
-        cja = dataset_collection.creating_job_associations
-        if cja:
+        if cja := dataset_collection.creating_job_associations:
             # Use the "first" job to represent all mapped jobs.
             representative_assoc = cja[0]
             representative_job = representative_assoc.job
diff --git a/lib/galaxy/workflow/modules.py b/lib/galaxy/workflow/modules.py
index 4b5f18bee32c..6105111dce89 100644
--- a/lib/galaxy/workflow/modules.py
+++ b/lib/galaxy/workflow/modules.py
@@ -313,8 +313,7 @@ def get_state(self, nested=True):
         """Return a serializable representation of the persistable state of
         the step.
         """
-        inputs = self.get_inputs()
-        if inputs:
+        if inputs := self.get_inputs():
             return self.state.encode(Bunch(inputs=inputs), self.trans.app, nested=nested)
         else:
             return self.state.inputs
@@ -347,8 +346,7 @@ def recover_state(self, state, **kwds):
             state = self.step_state_to_tool_state(state or {})
 
         self.state = DefaultToolState()
-        inputs = self.get_inputs()
-        if inputs:
+        if inputs := self.get_inputs():
             self.state.decode(state, Bunch(inputs=inputs), self.trans.app)
         else:
             self.state.inputs = safe_loads(state) or {}
@@ -1114,7 +1112,6 @@ def get_runtime_inputs(self, step, connections: Optional[Iterable[WorkflowStepCo
         collection_type = parameter_def["collection_type"]
         optional = parameter_def["optional"]
         tag = parameter_def["tag"]
-        formats = parameter_def.get("format")
         collection_param_source = dict(
             name="input",
             label=self.label,
@@ -1123,7 +1120,7 @@ def get_runtime_inputs(self, step, connections: Optional[Iterable[WorkflowStepCo
             tag=tag,
             optional=optional,
         )
-        if formats:
+        if formats := parameter_def.get("format"):
             collection_param_source["format"] = ",".join(listify(formats))
         input_param = DataCollectionToolParameter(None, collection_param_source, self.trans)
         return dict(input=input_param)
@@ -1148,8 +1145,7 @@ def get_all_outputs(self, data_only=False):
 
     def _parse_state_into_dict(self):
         state_as_dict = super()._parse_state_into_dict()
-        inputs = self.state.inputs
-        if "collection_type" in inputs:
+        if "collection_type" in (inputs := self.state.inputs):
             collection_type = inputs["collection_type"]
         else:
             collection_type = self.default_collection_type
@@ -1824,8 +1820,7 @@ def save_to_step(self, step, detached=False):
             step.tool_version = self.get_version()
         else:
             step.tool_version = self.tool_version
-        tool_uuid = getattr(self, "tool_uuid", None)
-        if tool_uuid:
+        if tool_uuid := getattr(self, "tool_uuid", None):
             step.dynamic_tool = self.trans.app.dynamic_tool_manager.get_tool_by_uuid(tool_uuid)
         if not detached:
             for k, v in self.post_job_actions.items():
diff --git a/lib/galaxy/workflow/run_request.py b/lib/galaxy/workflow/run_request.py
index a188dee32b2b..8797b1fb8fe1 100644
--- a/lib/galaxy/workflow/run_request.py
+++ b/lib/galaxy/workflow/run_request.py
@@ -202,8 +202,7 @@ def _step_parameters(step: "WorkflowStep", param_map: Dict, legacy: bool = False
         param_dict.update(param_map.get(str(step.id), {}))
     else:
         param_dict.update(param_map.get(str(step.order_index), {}))
-    step_uuid = step.uuid
-    if step_uuid:
+    if step_uuid := step.uuid:
         uuid_params = param_map.get(str(step_uuid), {})
         param_dict.update(uuid_params)
     if param_dict:
diff --git a/lib/galaxy/workflow/trs_proxy.py b/lib/galaxy/workflow/trs_proxy.py
index a9a32909457a..7bf65631b46a 100644
--- a/lib/galaxy/workflow/trs_proxy.py
+++ b/lib/galaxy/workflow/trs_proxy.py
@@ -74,8 +74,7 @@ def server_from_url(self, trs_url):
         return TrsServer(trs_url)
 
     def match_url(self, url):
-        matches = re.match(TRS_URL_REGEX, url)
-        if matches:
+        if matches := re.match(TRS_URL_REGEX, url):
             match_dict = matches.groupdict()
             match_dict["tool_id"] = urllib.parse.unquote(match_dict["tool_id"])
             return match_dict
diff --git a/lib/galaxy_test/api/test_workflow_extraction.py b/lib/galaxy_test/api/test_workflow_extraction.py
index f8f5c939852e..7b964223df19 100644
--- a/lib/galaxy_test/api/test_workflow_extraction.py
+++ b/lib/galaxy_test/api/test_workflow_extraction.py
@@ -495,9 +495,7 @@ def _assert_first_step_is_paired_input(self, downloaded_workflow):
         return collect_step_idx
 
     def _extract_and_download_workflow(self, history_id: str, **extract_payload):
-        reimport_as = extract_payload.get("reimport_as")
-
-        if reimport_as:
+        if reimport_as := extract_payload.get("reimport_as"):
             history_name = reimport_as
             self.dataset_populator.wait_for_history(history_id)
             self.dataset_populator.rename_history(history_id, history_name)
diff --git a/lib/galaxy_test/base/api.py b/lib/galaxy_test/base/api.py
index 8198ae1b8ef0..964494a3dfc2 100644
--- a/lib/galaxy_test/base/api.py
+++ b/lib/galaxy_test/base/api.py
@@ -109,8 +109,7 @@ def _api_url(self, path, params=None, use_key=None, use_admin_key=None):
             params["key"] = self.galaxy_interactor.api_key
         if use_admin_key:
             params["key"] = self.galaxy_interactor.master_api_key
-        query = urlencode(params)
-        if query:
+        if query := urlencode(params):
             url = f"{url}?{query}"
         return url
 
diff --git a/lib/galaxy_test/base/decorators.py b/lib/galaxy_test/base/decorators.py
index a724c10d696d..710f7eda54ea 100644
--- a/lib/galaxy_test/base/decorators.py
+++ b/lib/galaxy_test/base/decorators.py
@@ -43,8 +43,7 @@ def using_requirement(tag: KnownRequirementT):
     """
     requirement = f"requires_{tag}"
     skip_environment_variable = f"GALAXY_TEST_SKIP_IF_{requirement.upper()}"
-    env_value = os.environ.get(skip_environment_variable, "0")
-    if env_value != "0":
+    if (env_value := os.environ.get(skip_environment_variable, "0")) != "0":
         raise unittest.SkipTest(f"[{env_value}] Skipping due to {skip_environment_variable} being set to {env_value}")
 
 
diff --git a/lib/galaxy_test/base/populators.py b/lib/galaxy_test/base/populators.py
index 86cdba03f8e3..09c6b7244da0 100644
--- a/lib/galaxy_test/base/populators.py
+++ b/lib/galaxy_test/base/populators.py
@@ -1308,8 +1308,7 @@ def get_export_url(self, export_url) -> Response:
 
     def import_history(self, import_data):
         files = {}
-        archive_file = import_data.pop("archive_file", None)
-        if archive_file:
+        if archive_file := import_data.pop("archive_file", None):
             files["archive_file"] = archive_file
         import_response = self._post("histories", data=import_data, files=files)
         api_asserts.assert_status_code_is(import_response, 200)
diff --git a/lib/galaxy_test/driver/driver_util.py b/lib/galaxy_test/driver/driver_util.py
index de9712700633..ee79b007f000 100644
--- a/lib/galaxy_test/driver/driver_util.py
+++ b/lib/galaxy_test/driver/driver_util.py
@@ -854,8 +854,7 @@ def stop_servers(self):
             server_wrapper.stop()
         for th in threading.enumerate():
             log.debug(f"After stopping all servers thread {th} is alive.")
-        active_count = threading.active_count()
-        if active_count > 100:
+        if (active_count := threading.active_count()) > 100:
             # For an unknown reason running iRODS tests results in application threads not shutting down immediately,
             # but if we've accumulated over 100 active threads something else is wrong that needs to be fixed.
             raise Exception(
diff --git a/lib/galaxy_test/driver/integration_util.py b/lib/galaxy_test/driver/integration_util.py
index 2d9309b083ec..1a38759c13f2 100644
--- a/lib/galaxy_test/driver/integration_util.py
+++ b/lib/galaxy_test/driver/integration_util.py
@@ -130,8 +130,7 @@ def tearDownClass(cls):
         cls._app_available = False
 
     def tearDown(self):
-        logs = self._test_driver.get_logs()
-        if logs:
+        if logs := self._test_driver.get_logs():
             print(logs)
         return super().tearDown()
 
diff --git a/lib/galaxy_test/selenium/framework.py b/lib/galaxy_test/selenium/framework.py
index 2e5b67c14522..23d18b3f7478 100644
--- a/lib/galaxy_test/selenium/framework.py
+++ b/lib/galaxy_test/selenium/framework.py
@@ -587,8 +587,7 @@ def assert_item_hid_text(self, hid):
 class UsesWorkflowAssertions(NavigatesGalaxyMixin):
     @retry_assertion_during_transitions
     def _assert_showing_n_workflows(self, n):
-        actual_count = len(self.workflow_index_table_elements())
-        if actual_count != n:
+        if (actual_count := len(self.workflow_index_table_elements())) != n:
             message = f"Expected {n} workflows to be displayed, based on DOM found {actual_count} workflow rows."
             raise AssertionError(message)
 
diff --git a/lib/galaxy_test/selenium/test_pages_index.py b/lib/galaxy_test/selenium/test_pages_index.py
index f7f82676b7e6..baca1d802e97 100644
--- a/lib/galaxy_test/selenium/test_pages_index.py
+++ b/lib/galaxy_test/selenium/test_pages_index.py
@@ -28,7 +28,6 @@ def new_page(self):
 
     @retry_assertion_during_transitions
     def _assert_showing_n_pages(self, n):
-        actual_count = len(self.pages_index_table_elements())
-        if actual_count != n:
+        if (actual_count := len(self.pages_index_table_elements())) != n:
             message = f"Expected {n} pages to be displayed, based on DOM found {actual_count} page index rows."
             raise AssertionError(message)
diff --git a/lib/galaxy_test/selenium/test_tool_form.py b/lib/galaxy_test/selenium/test_tool_form.py
index 9dba37e40697..a70bae70f2b6 100644
--- a/lib/galaxy_test/selenium/test_tool_form.py
+++ b/lib/galaxy_test/selenium/test_tool_form.py
@@ -285,8 +285,7 @@ def test_bibtex_rendering(self):
         @retry_assertion_during_transitions
         def assert_citations_visible():
             references = self.components.tool_form.reference.all()
-            references_rendered = len(references)
-            if references_rendered != citation_count:
+            if (references_rendered := len(references)) != citation_count:
                 citations_api = self.api_get("tools/bibtex/citations")
                 current_citation_count = len(citations_api)
                 message = f"Expected {citation_count} references to be rendered, {references_rendered} actually rendered. Currently the API yields {current_citation_count} references"
diff --git a/lib/tool_shed/dependencies/repository/relation_builder.py b/lib/tool_shed/dependencies/repository/relation_builder.py
index 33811377d031..416282f78055 100644
--- a/lib/tool_shed/dependencies/repository/relation_builder.py
+++ b/lib/tool_shed/dependencies/repository/relation_builder.py
@@ -156,8 +156,7 @@ def get_repository_dependencies_for_changeset_revision(self):
         """
         # Assume the current repository does not have repository dependencies defined for it.
         current_repository_key = None
-        metadata = self.repository_metadata.metadata
-        if metadata:
+        if metadata := self.repository_metadata.metadata:
             # The value of self.tool_shed_url must include the port, but doesn't have to include
             # the protocol.
             if "repository_dependencies" in metadata:
diff --git a/lib/tool_shed/grids/repository_grids.py b/lib/tool_shed/grids/repository_grids.py
index a61de72dc285..5c80c185ff38 100644
--- a/lib/tool_shed/grids/repository_grids.py
+++ b/lib/tool_shed/grids/repository_grids.py
@@ -352,9 +352,8 @@ def get_value(self, trans, grid, repository_metadata):
     use_paging = False
 
     def build_initial_query(self, trans, **kwd):
-        match_tuples = kwd.get("match_tuples", [])
         clause_list = []
-        if match_tuples:
+        if match_tuples := kwd.get("match_tuples", []):
             for match_tuple in match_tuples:
                 repository_id, changeset_revision = match_tuple
                 clause_list.append(
@@ -856,8 +855,7 @@ def get_value(self, trans, grid, repository):
                 trans, repository
             )
             metadata = repository_metadata.metadata
-            invalid_tools = metadata.get("invalid_tools", [])
-            if invalid_tools:
+            if invalid_tools := metadata.get("invalid_tools", []):
                 for invalid_tool_config in invalid_tools:
                     href_str = '<a href="load_invalid_tool?repository_id={}&tool_config={}&changeset_revision={}">{}</a>'.format(
                         trans.security.encode_id(repository.id),
diff --git a/lib/tool_shed/managers/repositories.py b/lib/tool_shed/managers/repositories.py
index c25918547b98..62f145f3aa56 100644
--- a/lib/tool_shed/managers/repositories.py
+++ b/lib/tool_shed/managers/repositories.py
@@ -458,8 +458,7 @@ def create_repository(trans: ProvidesUserContext, request: CreateRepositoryReque
     assert user
     category_ids = listify(request.category_ids)
     name = request.name
-    invalid_message = validate_repository_name(app, name, user)
-    if invalid_message:
+    if invalid_message := validate_repository_name(app, name, user):
         raise RequestParameterInvalidException(invalid_message)
 
     repo, _ = low_level_create_repository(
diff --git a/lib/tool_shed/managers/trs.py b/lib/tool_shed/managers/trs.py
index fbd1880b60b1..3c5cfb9e89e1 100644
--- a/lib/tool_shed/managers/trs.py
+++ b/lib/tool_shed/managers/trs.py
@@ -56,9 +56,8 @@ def service_info(app: ToolShedApp, request_url: URL):
         artifact="trs",
         version="2.1.0",
     )
-    environment = config.ga4gh_service_environment
     extra_kwds = {}
-    if environment:
+    if environment := config.ga4gh_service_environment:
         extra_kwds["environment"] = environment
     return Service(
         id=organization_id + ".trs",
diff --git a/lib/tool_shed/metadata/repository_metadata_manager.py b/lib/tool_shed/metadata/repository_metadata_manager.py
index 2bc5e756a0d0..a5df2eddcf91 100644
--- a/lib/tool_shed/metadata/repository_metadata_manager.py
+++ b/lib/tool_shed/metadata/repository_metadata_manager.py
@@ -918,10 +918,9 @@ def reset_metadata_on_selected_repositories(self, **kwd):
         Inspect the repository changelog to reset metadata for all appropriate changeset revisions.
         This method is called from both Galaxy and the Tool Shed.
         """
-        repository_ids = util.listify(kwd.get("repository_ids", None))
         message = ""
         status = "done"
-        if repository_ids:
+        if repository_ids := util.listify(kwd.get("repository_ids", None)):
             successful_count = 0
             unsuccessful_count = 0
             for repository_id in repository_ids:
diff --git a/lib/tool_shed/test/base/twillbrowser.py b/lib/tool_shed/test/base/twillbrowser.py
index 65cf8c48eda3..1f4af8791678 100644
--- a/lib/tool_shed/test/base/twillbrowser.py
+++ b/lib/tool_shed/test/base/twillbrowser.py
@@ -14,6 +14,8 @@
 )
 
 tc.options["equiv_refresh_interval"] = 0
+# Resetting all repository metadata can take a really long time
+tc.timeout(240)
 
 
 def visit_url(url: str, allowed_codes: List[int]) -> str:
diff --git a/lib/tool_shed/util/hg_util.py b/lib/tool_shed/util/hg_util.py
index 965013846c4f..a0225c0d1a05 100644
--- a/lib/tool_shed/util/hg_util.py
+++ b/lib/tool_shed/util/hg_util.py
@@ -151,8 +151,7 @@ def get_revision_label(app, repository, changeset_revision, include_date=True, i
     which includes the revision date if the receive include_date is True.
     """
     repo = repository.hg_repo
-    ctx = get_changectx_for_changeset(repo, changeset_revision)
-    if ctx:
+    if ctx := get_changectx_for_changeset(repo, changeset_revision):
         return get_revision_label_from_ctx(ctx, include_date=include_date, include_hash=include_hash)
     else:
         if include_hash:
@@ -168,8 +167,7 @@ def get_rev_label_changeset_revision_from_repository_metadata(
         repository = repository_metadata.repository
     repo = repository.hg_repo
     changeset_revision = repository_metadata.changeset_revision
-    ctx = get_changectx_for_changeset(repo, changeset_revision)
-    if ctx:
+    if ctx := get_changectx_for_changeset(repo, changeset_revision):
         rev = "%04d" % ctx.rev()
         if include_date:
             changeset_revision_date = get_readable_ctx_date(ctx)
@@ -209,8 +207,7 @@ def get_rev_label_from_changeset_revision(repo, changeset_revision, include_date
     Given a changeset revision hash, return two strings, the changeset rev and the changeset revision hash
     which includes the revision date if the receive include_date is True.
     """
-    ctx = get_changectx_for_changeset(repo, changeset_revision)
-    if ctx:
+    if ctx := get_changectx_for_changeset(repo, changeset_revision):
         rev = "%04d" % ctx.rev()
         label = get_revision_label_from_ctx(ctx, include_date=include_date)
     else:
diff --git a/lib/tool_shed/util/shed_util_common.py b/lib/tool_shed/util/shed_util_common.py
index ed4d160effda..99027ea17fc8 100644
--- a/lib/tool_shed/util/shed_util_common.py
+++ b/lib/tool_shed/util/shed_util_common.py
@@ -318,8 +318,7 @@ def is_path_within_dependency_dir(app: "ToolShedApp", path: str) -> bool:
     """
     allowed = False
     resolved_path = os.path.realpath(path)
-    tool_dependency_dir = app.config.get("tool_dependency_dir", None)
-    if tool_dependency_dir:
+    if tool_dependency_dir := app.config.get("tool_dependency_dir", None):
         dependency_path = os.path.abspath(tool_dependency_dir)
         allowed = os.path.commonprefix([dependency_path, resolved_path]) == dependency_path
     return allowed
diff --git a/lib/tool_shed/webapp/api/groups.py b/lib/tool_shed/webapp/api/groups.py
index 14228462e675..3fd412dc7211 100644
--- a/lib/tool_shed/webapp/api/groups.py
+++ b/lib/tool_shed/webapp/api/groups.py
@@ -83,8 +83,7 @@ def create(self, trans, payload, **kwd):
         Content-Disposition: form-data; name="description" Group_Description
         """
         group_dict = dict(message="", status="ok")
-        name = payload.get("name", "")
-        if name:
+        if name := payload.get("name", ""):
             description = payload.get("description", "")
             if not description:
                 description = ""
diff --git a/lib/tool_shed/webapp/api/repositories.py b/lib/tool_shed/webapp/api/repositories.py
index 740f3a995421..713452202ca5 100644
--- a/lib/tool_shed/webapp/api/repositories.py
+++ b/lib/tool_shed/webapp/api/repositories.py
@@ -214,8 +214,7 @@ def get_installable_revisions(self, trans, **kwd):
         Returns a list of lists of changesets, in the format [ [ 0, fbb391dc803c ], [ 1, 9d9ec4d9c03e ], [ 2, 9b5b20673b89 ], [ 3, e8c99ce51292 ] ].
         """
         # Example URL: http://localhost:9009/api/repositories/get_installable_revisions?tsr_id=9d37e53072ff9fa4
-        tsr_id = kwd.get("tsr_id", None)
-        if tsr_id is not None:
+        if (tsr_id := kwd.get("tsr_id", None)) is not None:
             repository = repository_util.get_repository_in_tool_shed(
                 self.app, tsr_id, eagerload_columns=[model.Repository.downloadable_revisions]
             )
@@ -271,8 +270,7 @@ def index(self, trans, deleted=False, owner=None, name=None, **kwd):
         """
         repository_dicts = []
         deleted = util.asbool(deleted)
-        q = kwd.get("q", "")
-        if q:
+        if q := kwd.get("q", ""):
             page = kwd.get("page", 1)
             page_size = kwd.get("page_size", 10)
             try:
@@ -288,8 +286,7 @@ def index(self, trans, deleted=False, owner=None, name=None, **kwd):
             else:
                 response = json.dumps(search_results)
             return response
-        tool_ids = kwd.get("tool_ids", None)
-        if tool_ids is not None:
+        if (tool_ids := kwd.get("tool_ids", None)) is not None:
             tool_ids = util.listify(tool_ids)
             response = index_tool_ids(self.app, tool_ids)
             return json.dumps(response)
diff --git a/lib/tool_shed/webapp/api2/__init__.py b/lib/tool_shed/webapp/api2/__init__.py
index 3630038280f3..845e1a9a2cfc 100644
--- a/lib/tool_shed/webapp/api2/__init__.py
+++ b/lib/tool_shed/webapp/api2/__init__.py
@@ -291,7 +291,6 @@ def ensure_valid_session(trans: SessionRequestContext) -> None:
     sa_session = app.model.context
     request = trans.request
     # Try to load an existing session
-    secure_id = request.get_cookie(AUTH_COOKIE_NAME)
     galaxy_session = None
     prev_galaxy_session = None
     user_for_new_session = None
@@ -299,7 +298,7 @@ def ensure_valid_session(trans: SessionRequestContext) -> None:
     # Track whether the session has changed so we can avoid calling flush
     # in the most common case (session exists and is valid).
     galaxy_session_requires_flush = False
-    if secure_id:
+    if secure_id := request.get_cookie(AUTH_COOKIE_NAME):
         session_key: Optional[str] = app.security.decode_guid(secure_id)
         if session_key:
             # We do NOT catch exceptions here, if the database is down the request should fail,
diff --git a/lib/tool_shed/webapp/controllers/admin.py b/lib/tool_shed/webapp/controllers/admin.py
index aec70e430121..73ddef546528 100644
--- a/lib/tool_shed/webapp/controllers/admin.py
+++ b/lib/tool_shed/webapp/controllers/admin.py
@@ -173,8 +173,7 @@ def create_category(self, trans, **kwd):
     def delete_repository(self, trans, **kwd):
         message = escape(kwd.get("message", ""))
         status = kwd.get("status", "done")
-        id = kwd.get("id", None)
-        if id:
+        if id := kwd.get("id", None):
             # Deleting multiple items is currently not allowed (allow_multiple=False), so there will only be 1 id.
             ids = util.listify(id)
             count = 0
@@ -222,8 +221,7 @@ def delete_repository(self, trans, **kwd):
     def delete_repository_metadata(self, trans, **kwd):
         message = escape(kwd.get("message", ""))
         status = kwd.get("status", "done")
-        id = kwd.get("id", None)
-        if id:
+        if id := kwd.get("id", None):
             ids = util.listify(id)
             count = 0
             for repository_metadata_id in ids:
@@ -382,8 +380,7 @@ def reset_metadata_on_selected_repositories_in_tool_shed(self, trans, **kwd):
     @web.require_admin
     def undelete_repository(self, trans, **kwd):
         message = escape(kwd.get("message", ""))
-        id = kwd.get("id", None)
-        if id:
+        if id := kwd.get("id", None):
             # Undeleting multiple items is currently not allowed (allow_multiple=False), so there will only be 1 id.
             ids = util.listify(id)
             count = 0
@@ -437,8 +434,7 @@ def mark_category_deleted(self, trans, **kwd):
         # sense to mark a category as deleted (category names and descriptions can be changed instead).
         # If we do this, and the following 2 methods can be eliminated.
         message = escape(kwd.get("message", ""))
-        id = kwd.get("id", None)
-        if id:
+        if id := kwd.get("id", None):
             ids = util.listify(id)
             message = "Deleted %d categories: " % len(ids)
             for category_id in ids:
@@ -465,8 +461,7 @@ def purge_category(self, trans, **kwd):
         # Purging a deleted Category deletes all of the following from the database:
         # - RepoitoryCategoryAssociations where category_id == Category.id
         message = escape(kwd.get("message", ""))
-        id = kwd.get("id", None)
-        if id:
+        if id := kwd.get("id", None):
             ids = util.listify(id)
             count = 0
             purged_categories = ""
@@ -493,8 +488,7 @@ def purge_category(self, trans, **kwd):
     @web.require_admin
     def undelete_category(self, trans, **kwd):
         message = escape(kwd.get("message", ""))
-        id = kwd.get("id", None)
-        if id:
+        if id := kwd.get("id", None):
             ids = util.listify(id)
             count = 0
             undeleted_categories = ""
diff --git a/lib/tool_shed/webapp/controllers/repository.py b/lib/tool_shed/webapp/controllers/repository.py
index 688e2397525b..d471402aa7d1 100644
--- a/lib/tool_shed/webapp/controllers/repository.py
+++ b/lib/tool_shed/webapp/controllers/repository.py
@@ -199,8 +199,7 @@ def browse_deprecated_repositories_i_own(self, trans, **kwd):
 
     @web.expose
     def browse_my_writable_repositories(self, trans, **kwd):
-        _redir = self._redirect_if_necessary(trans, **kwd)
-        if _redir is not None:
+        if (_redir := self._redirect_if_necessary(trans, **kwd)) is not None:
             return _redir
 
         selected_changeset_revision, repository = suc.get_repository_from_refresh_on_change(trans.app, **kwd)
@@ -218,8 +217,7 @@ def browse_my_writable_repositories(self, trans, **kwd):
 
     @web.expose
     def browse_my_writable_repositories_missing_tool_test_components(self, trans, **kwd):
-        _redir = self._redirect_if_necessary(trans, **kwd)
-        if _redir is not None:
+        if (_redir := self._redirect_if_necessary(trans, **kwd)) is not None:
             return _redir
 
         if "message" not in kwd:
@@ -237,8 +235,7 @@ def browse_my_writable_repositories_missing_tool_test_components(self, trans, **
 
     @web.expose
     def browse_my_writable_repositories_with_invalid_tools(self, trans, **kwd):
-        _redir = self._redirect_if_necessary(trans, **kwd)
-        if _redir is not None:
+        if (_redir := self._redirect_if_necessary(trans, **kwd)) is not None:
             return _redir
 
         if "message" not in kwd:
@@ -360,8 +357,7 @@ def browse_repositories_by_user(self, trans, **kwd):
 
     @web.expose
     def browse_repositories_i_can_administer(self, trans, **kwd):
-        _redir = self._redirect_if_necessary(trans, **kwd)
-        if _redir is not None:
+        if (_redir := self._redirect_if_necessary(trans, **kwd)) is not None:
             return _redir
 
         selected_changeset_revision, repository = suc.get_repository_from_refresh_on_change(trans.app, **kwd)
@@ -379,8 +375,7 @@ def browse_repositories_i_can_administer(self, trans, **kwd):
 
     @web.expose
     def browse_repositories_i_own(self, trans, **kwd):
-        _redir = self._redirect_if_necessary(trans, **kwd)
-        if _redir is not None:
+        if (_redir := self._redirect_if_necessary(trans, **kwd)) is not None:
             return _redir
 
         selected_changeset_revision, repository = suc.get_repository_from_refresh_on_change(trans.app, **kwd)
@@ -427,8 +422,7 @@ def browse_repositories_in_category(self, trans, **kwd):
                     changeset_revision=selected_changeset_revision,
                 )
             )
-        category_id = kwd.get("id", None)
-        if category_id:
+        if category_id := kwd.get("id", None):
             category = suc.get_category(trans.app, category_id)
             if category:
                 trailing_string = f"in Category {str(category.name)}"
@@ -444,8 +438,7 @@ def browse_repositories_in_category(self, trans, **kwd):
 
     @web.expose
     def browse_repositories_missing_tool_test_components(self, trans, **kwd):
-        _redir = self._redirect_if_necessary(trans, **kwd)
-        if _redir is not None:
+        if (_redir := self._redirect_if_necessary(trans, **kwd)) is not None:
             return _redir
 
         if "message" not in kwd:
@@ -462,8 +455,7 @@ def browse_repositories_missing_tool_test_components(self, trans, **kwd):
 
     @web.expose
     def browse_repositories_with_invalid_tools(self, trans, **kwd):
-        _redir = self._redirect_if_necessary(trans, **kwd)
-        if _redir is not None:
+        if (_redir := self._redirect_if_necessary(trans, **kwd)) is not None:
             return _redir
 
         if "message" not in kwd:
@@ -557,8 +549,7 @@ def browse_tool_dependencies(self, trans, **kwd):
     def browse_valid_categories(self, trans, **kwd):
         """Filter repositories per category by those that are valid for installing into Galaxy."""
         # The request came from Galaxy, so restrict category links to display only valid repository changeset revisions.
-        galaxy_url = common_util.handle_galaxy_url(trans, **kwd)
-        if galaxy_url:
+        if galaxy_url := common_util.handle_galaxy_url(trans, **kwd):
             kwd["galaxy_url"] = galaxy_url
         if "f-free-text-search" in kwd:
             if kwd["f-free-text-search"] == "All":
@@ -595,8 +586,7 @@ def browse_valid_categories(self, trans, **kwd):
     @web.expose
     def browse_valid_repositories(self, trans, **kwd):
         """Filter repositories to those that are installable into Galaxy."""
-        galaxy_url = common_util.handle_galaxy_url(trans, **kwd)
-        if galaxy_url:
+        if galaxy_url := common_util.handle_galaxy_url(trans, **kwd):
             kwd["galaxy_url"] = galaxy_url
         repository_id = kwd.get("id", None)
         if "f-free-text-search" in kwd:
@@ -1152,8 +1142,7 @@ def get_ctx_rev(self, trans, **kwd):
         changeset_revision = kwd["changeset_revision"]
         repository = repository_util.get_repository_by_name_and_owner(trans.app, repository_name, repository_owner)
         repo = repository.hg_repo
-        ctx = hg_util.get_changectx_for_changeset(repo, changeset_revision)
-        if ctx:
+        if ctx := hg_util.get_changectx_for_changeset(repo, changeset_revision):
             return str(ctx.rev())
         return ""
 
@@ -1428,8 +1417,7 @@ def get_updated_repository_information(self, trans, name, owner, changeset_revis
         includes_tools_for_display_in_tool_panel = False
         includes_workflows = False
         readme_files_dict = None
-        metadata = repository_metadata.metadata
-        if metadata:
+        if metadata := repository_metadata.metadata:
             if "data_manager" in metadata:
                 includes_data_managers = True
             if "datatypes" in metadata:
@@ -1505,12 +1493,11 @@ def index(self, trans, **kwd):
         status = kwd.get("status", "done")
         # See if there are any RepositoryMetadata records since menu items require them.
         repository_metadata = get_first_repository_metadata(trans.sa_session)
-        current_user = trans.user
         # TODO: move the following to some in-memory register so these queries can be done once
         # at startup.  The in-memory register can then be managed during the current session.
         can_administer_repositories = False
         has_deprecated_repositories = False
-        if current_user:
+        if current_user := trans.user:
             # See if the current user has any repositories that have been marked as deprecated.
             for repository in current_user.active_repositories:
                 if repository.deprecated:
@@ -1727,8 +1714,7 @@ def manage_repository(self, trans, id, **kwd):
         if error:
             status = "error"
         allow_push_select_field = SelectField(name="allow_push", multiple=True)
-        current_allow_push = repository.allow_push()
-        if current_allow_push:
+        if current_allow_push := repository.allow_push():
             current_allow_push_list = current_allow_push.split(",")
         else:
             current_allow_push_list = []
@@ -2118,8 +2104,7 @@ def reset_all_metadata(self, trans, id, **kwd):
         )
         rmm.reset_all_metadata_on_repository_in_tool_shed()
         rmm_metadata_dict = rmm.get_metadata_dict()
-        rmm_invalid_file_tups = rmm.get_invalid_file_tups()
-        if rmm_invalid_file_tups:
+        if rmm_invalid_file_tups := rmm.get_invalid_file_tups():
             message = tool_util.generate_message_for_invalid_tools(
                 trans.app, rmm_invalid_file_tups, repository, rmm_metadata_dict
             )
@@ -2155,8 +2140,7 @@ def set_email_alerts(self, trans, **kwd):
         """Set email alerts for selected repositories."""
         # This method is called from multiple grids, so the caller must be passed.
         caller = kwd["caller"]
-        user = trans.user
-        if user:
+        if user := trans.user:
             repository_ids = util.listify(kwd.get("id", ""))
             total_alerts_added = 0
             total_alerts_removed = 0
@@ -2450,8 +2434,7 @@ def view_changeset(self, trans, id, ctx_str, **kwd):
 
     @web.expose
     def view_or_manage_repository(self, trans, **kwd):
-        repository_id = kwd.get("id", None)
-        if repository_id:
+        if repository_id := kwd.get("id", None):
             repository = repository_util.get_repository_in_tool_shed(trans.app, repository_id)
             user = trans.user
             if repository:
diff --git a/lib/tool_shed/webapp/frontend/src/schema/schema.ts b/lib/tool_shed/webapp/frontend/src/schema/schema.ts
index c49abf299742..399b26a1e64c 100644
--- a/lib/tool_shed/webapp/frontend/src/schema/schema.ts
+++ b/lib/tool_shed/webapp/frontend/src/schema/schema.ts
@@ -290,7 +290,7 @@ export interface components {
              * Type
              * @description The digest method used to create the checksum.
              * The value (e.g. `sha-256`) SHOULD be listed as `Hash Name String` in the https://github.com/ga4gh-discovery/ga4gh-checksum/blob/master/hash-alg.csv[GA4GH Checksum Hash Algorithm Registry].
-             * Other values MAY be used, as long as implementors are aware of the issues discussed in https://tools.ietf.org/html/rfc6920#section-9.4[RFC6920].
+             * Other values MAY be used, as long as implementers are aware of the issues discussed in https://tools.ietf.org/html/rfc6920#section-9.4[RFC6920].
              * GA4GH may provide more explicit guidance for use of non-IANA-registered algorithms in the future.
              */
             type: string
diff --git a/lib/tool_shed/webapp/model/__init__.py b/lib/tool_shed/webapp/model/__init__.py
index f748f7938735..2f2be9dccfbe 100644
--- a/lib/tool_shed/webapp/model/__init__.py
+++ b/lib/tool_shed/webapp/model/__init__.py
@@ -168,8 +168,7 @@ def set_disk_usage(self, bytes):
     total_disk_usage = property(get_disk_usage, set_disk_usage)
 
     def set_password_cleartext(self, cleartext):
-        message = validate_password_str(cleartext)
-        if message:
+        if message := validate_password_str(cleartext):
             raise Exception(f"Invalid password: {message}")
         # Set 'self.password' to the digest of 'cleartext'.
         self.password = new_insecure_hash(text_type=cleartext)
diff --git a/lib/tool_shed/webapp/security/__init__.py b/lib/tool_shed/webapp/security/__init__.py
index 8bf4d52fbdb7..ce61f1dba1dd 100644
--- a/lib/tool_shed/webapp/security/__init__.py
+++ b/lib/tool_shed/webapp/security/__init__.py
@@ -273,8 +273,7 @@ def user_can_import_repository_archive(self, user, archive_owner):
         if user.username == archive_owner:
             return True
         # A member of the IUC is authorized to create new repositories that are owned by another user.
-        iuc_group = get_iuc_group(self.sa_session)
-        if iuc_group is not None:
+        if (iuc_group := get_iuc_group(self.sa_session)) is not None:
             for uga in iuc_group.users:
                 if uga.user.id == user.id:
                     return True
diff --git a/lib/tool_shed_client/schema/trs.py b/lib/tool_shed_client/schema/trs.py
index fceff90e76f7..8d9984800b7f 100644
--- a/lib/tool_shed_client/schema/trs.py
+++ b/lib/tool_shed_client/schema/trs.py
@@ -22,7 +22,7 @@ class Checksum(BaseModel):
     checksum: str = Field(..., description="The hex-string encoded checksum for the data. ")
     type: str = Field(
         ...,
-        description="The digest method used to create the checksum.\nThe value (e.g. `sha-256`) SHOULD be listed as `Hash Name String` in the https://github.com/ga4gh-discovery/ga4gh-checksum/blob/master/hash-alg.csv[GA4GH Checksum Hash Algorithm Registry].\nOther values MAY be used, as long as implementors are aware of the issues discussed in https://tools.ietf.org/html/rfc6920#section-9.4[RFC6920].\nGA4GH may provide more explicit guidance for use of non-IANA-registered algorithms in the future.",
+        description="The digest method used to create the checksum.\nThe value (e.g. `sha-256`) SHOULD be listed as `Hash Name String` in the https://github.com/ga4gh-discovery/ga4gh-checksum/blob/master/hash-alg.csv[GA4GH Checksum Hash Algorithm Registry].\nOther values MAY be used, as long as implementers are aware of the issues discussed in https://tools.ietf.org/html/rfc6920#section-9.4[RFC6920].\nGA4GH may provide more explicit guidance for use of non-IANA-registered algorithms in the future.",
     )
 
 
diff --git a/mypy.ini b/mypy.ini
index 0676824eb3b9..e9da196321af 100644
--- a/mypy.ini
+++ b/mypy.ini
@@ -1,5 +1,4 @@
 [mypy]
-enable_incomplete_feature = Unpack
 plugins = pydantic.mypy
 show_error_codes = True
 ignore_missing_imports = True
diff --git a/packages/app/setup.cfg b/packages/app/setup.cfg
index c8cc845deed1..02a35b4e3afd 100644
--- a/packages/app/setup.cfg
+++ b/packages/app/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -51,10 +50,9 @@ install_requires =
     cloudauthz==0.6.0
     dparse
     gxformat2
-    importlib-metadata<5  # Work around https://github.com/celery/kombu/issues/1600
-    kombu
+    kombu>=5.3
     lagom
-    lxml
+    lxml!=4.2.2
     Mako
     Markdown
     MarkupSafe
@@ -77,7 +75,7 @@ install_requires =
     WebOb
     Whoosh
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.entry_points]
 console_scripts =
diff --git a/packages/auth/setup.cfg b/packages/auth/setup.cfg
index cca3a9b7ad7c..5dfbe3c629e2 100644
--- a/packages/auth/setup.cfg
+++ b/packages/auth/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -35,8 +34,8 @@ install_requires =
     galaxy-data
     galaxy-util
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/config/setup.cfg b/packages/config/setup.cfg
index 16d2815d2a2e..a4bbe8efb729 100644
--- a/packages/config/setup.cfg
+++ b/packages/config/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -38,7 +37,7 @@ install_requires =
     pykwalify
     PyYAML
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.entry_points]
 console_scripts =
@@ -46,4 +45,4 @@ console_scripts =
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/config/test-requirements.txt b/packages/config/test-requirements.txt
index a8797797f1cf..4340e1a6c3e8 100644
--- a/packages/config/test-requirements.txt
+++ b/packages/config/test-requirements.txt
@@ -1,2 +1,2 @@
-gravity
+gravity>=1.0
 pytest
diff --git a/packages/data/setup.cfg b/packages/data/setup.cfg
index 02f74386b9d5..94a5497e512f 100644
--- a/packages/data/setup.cfg
+++ b/packages/data/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -37,13 +36,13 @@ install_requires =
     galaxy-util[template]
     alembic
     alembic-utils
-    bdbag
+    bdbag>=1.6.3
     bx-python
     dnspython
     galaxy-sequence-utils
-    h5grove
+    h5grove>=1.2.1
     h5py
-    isa-rwval
+    isa-rwval>=0.10.10
     MarkupSafe
     msal
     mrcfile
@@ -61,7 +60,7 @@ install_requires =
     typing-extensions
     WebOb
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.entry_points]
 console_scripts =
diff --git a/packages/files/setup.cfg b/packages/files/setup.cfg
index 9f89c257b476..3f7f01acaf1b 100644
--- a/packages/files/setup.cfg
+++ b/packages/files/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -36,7 +35,7 @@ install_requires =
     fs
     typing-extensions
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
diff --git a/packages/files/test-requirements.txt b/packages/files/test-requirements.txt
index 000f71070273..ad572b378cec 100644
--- a/packages/files/test-requirements.txt
+++ b/packages/files/test-requirements.txt
@@ -1,3 +1,3 @@
 pytest
 fs-gcsfs
-s3fs
+s3fs>=2023.1.0,<2024
diff --git a/packages/job_execution/setup.cfg b/packages/job_execution/setup.cfg
index ea1943235b8a..1d77581d51ff 100644
--- a/packages/job_execution/setup.cfg
+++ b/packages/job_execution/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -40,7 +39,7 @@ install_requires =
     MarkupSafe
     SQLAlchemy>=1.4.25,<2
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.entry_points]
 console_scripts =
@@ -49,4 +48,4 @@ console_scripts =
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/meta/setup.cfg b/packages/meta/setup.cfg
index 516107f5088d..e3d3a152e651 100644
--- a/packages/meta/setup.cfg
+++ b/packages/meta/setup.cfg
@@ -10,7 +10,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -34,7 +33,7 @@ version = 22.5.0.dev0
 [options]
 include_package_data = True
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.extras_require]
 postgresql =
diff --git a/packages/navigation/setup.cfg b/packages/navigation/setup.cfg
index 3b16102ad68c..7b346eaa30cb 100644
--- a/packages/navigation/setup.cfg
+++ b/packages/navigation/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -35,8 +34,8 @@ install_requires =
     galaxy-util
     PyYAML
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/schema/setup.cfg b/packages/schema/setup.cfg
index 507f563909ea..eec616559910 100644
--- a/packages/schema/setup.cfg
+++ b/packages/schema/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -32,9 +31,9 @@ version = 23.1.0.dev0
 include_package_data = True
 install_requires =
     galaxy-util
-    pydantic[email]
+    pydantic[email]<2
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
diff --git a/packages/selenium/setup.cfg b/packages/selenium/setup.cfg
index a95b3a449dfa..338943d2013e 100644
--- a/packages/selenium/setup.cfg
+++ b/packages/selenium/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -39,7 +38,7 @@ install_requires =
     requests
     selenium!=4.11.0,!=4.11.1,!=4.11.2
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.entry_points]
 console_scripts =
@@ -47,4 +46,4 @@ console_scripts =
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/test_api/setup.cfg b/packages/test_api/setup.cfg
index 2a09121053d3..a759e952956e 100644
--- a/packages/test_api/setup.cfg
+++ b/packages/test_api/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -38,7 +37,7 @@ install_requires =
     requests
     tuspy
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.extras_require]
 driver =
@@ -46,4 +45,4 @@ driver =
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/test_base/setup.cfg b/packages/test_base/setup.cfg
index a1eec0ef392e..17c957eec67a 100644
--- a/packages/test_base/setup.cfg
+++ b/packages/test_base/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -41,8 +40,8 @@ install_requires =
     PyYAML
     requests
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/test_driver/setup.cfg b/packages/test_driver/setup.cfg
index cd64afd37a8c..9268dd3eb584 100644
--- a/packages/test_driver/setup.cfg
+++ b/packages/test_driver/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -43,8 +42,8 @@ install_requires =
     graphene-sqlalchemy==3.0.0b3  # these are only needed by tool shed - which we've split out but the test driver loads
     starlette-graphene3
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/test_selenium/setup.cfg b/packages/test_selenium/setup.cfg
index dca41568d040..177fbc9ba8ad 100644
--- a/packages/test_selenium/setup.cfg
+++ b/packages/test_selenium/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -43,7 +42,7 @@ install_requires =
     requests
     selenium!=4.11.0,!=4.11.1,!=4.11.2
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.extras_require]
 driver =
diff --git a/packages/tool_shed/setup.cfg b/packages/tool_shed/setup.cfg
index 50b32d282a99..304c61ed9f40 100644
--- a/packages/tool_shed/setup.cfg
+++ b/packages/tool_shed/setup.cfg
@@ -9,10 +9,10 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
+    Programming Language :: Python :: 3.11
     Topic :: Software Development
     Topic :: Software Development :: Code Generators
     Topic :: Software Development :: Testing
@@ -34,7 +34,7 @@ install_requires =
     galaxy-web-stack
     galaxy-web-apps
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
diff --git a/packages/tool_util/setup.cfg b/packages/tool_util/setup.cfg
index 817bd9080842..354417845ca6 100644
--- a/packages/tool_util/setup.cfg
+++ b/packages/tool_util/setup.cfg
@@ -34,7 +34,7 @@ include_package_data = True
 install_requires =
     galaxy-util>=22.1
     conda-package-streaming
-    lxml
+    lxml!=4.2.2
     MarkupSafe
     packaging
     pydantic<2
@@ -59,7 +59,7 @@ console_scripts =
 
 [options.extras_require]
 cwl =
-    cwltool==3.1.20221109155812
+    cwltool>=3.1.20230624081518
 mulled =
     jinja2
     Whoosh
diff --git a/packages/tours/setup.cfg b/packages/tours/setup.cfg
index 1186d0ad6ccf..999022f00af8 100644
--- a/packages/tours/setup.cfg
+++ b/packages/tours/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -36,7 +35,7 @@ install_requires =
     pydantic<2
     PyYAML
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.entry_points]
 console_scripts =
diff --git a/packages/util/setup.cfg b/packages/util/setup.cfg
index 03afa41b853b..39708d11975a 100644
--- a/packages/util/setup.cfg
+++ b/packages/util/setup.cfg
@@ -34,8 +34,8 @@ include_package_data = True
 install_requires =
     bleach
     boltons
-    docutils
-    importlib_resources
+    docutils!=0.17,!=0.17.1
+    importlib-resources;python_version<'3.9'
     packaging
     pyparsing
     PyYAML
@@ -50,7 +50,7 @@ python_requires = >=3.7
 jstree =
     dictobj
 template =
-    Cheetah3
+    Cheetah3!=3.2.6.post2
     future
 
 [options.packages.find]
diff --git a/packages/web_apps/setup.cfg b/packages/web_apps/setup.cfg
index 17de386f4c12..472f09cc1c85 100644
--- a/packages/web_apps/setup.cfg
+++ b/packages/web_apps/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -42,12 +41,12 @@ install_requires =
     a2wsgi
     apispec
     Babel
-    Cheetah3
-    fastapi>=0.68.2,!=0.69.0,!=0.70.0,!=0.70.1,<0.99
+    Cheetah3!=3.2.6.post2
+    fastapi>=0.71.0,!=0.89.0,<0.99
     fastapi-utils
     gunicorn
     gxformat2
-    importlib_resources
+    importlib-resources;python_version<'3.9'
     Mako
     MarkupSafe
     mercurial
@@ -70,7 +69,7 @@ install_requires =
     WebOb
     Whoosh
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
diff --git a/packages/web_framework/setup.cfg b/packages/web_framework/setup.cfg
index 99cce6a9e4c7..adceeced72ed 100644
--- a/packages/web_framework/setup.cfg
+++ b/packages/web_framework/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -44,7 +43,7 @@ install_requires =
     WebOb
 
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
diff --git a/packages/web_stack/setup.cfg b/packages/web_stack/setup.cfg
index 9d9e2022c398..6c1ff8aaee98 100644
--- a/packages/web_stack/setup.cfg
+++ b/packages/web_stack/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -36,8 +35,8 @@ install_requires =
     galaxy-util
     SQLAlchemy>=1.4.25,<2
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/pyproject.toml b/pyproject.toml
index ec98c6f49ef2..625d43195168 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -1,6 +1,6 @@
 [tool.black]
 line-length = 120
-target-version = ['py37']
+target-version = ['py38']
 include = '\.pyi?$'
 extend-exclude = '''
 ^/(
@@ -50,7 +50,7 @@ Cheetah3 = "!=3.2.6.post2"  # yanked release, https://github.com/python-poetry/p
 cloudauthz = "==0.6.0"
 cloudbridge = "*"
 circus = "*"
-cwltool = "==3.1.20221109155812"
+cwltool = ">=3.1.20230624081518"  # save time, minimum needed by cwl-1.0 branch
 conda-package-streaming = "*"
 dictobj = "*"
 dnspython = "*"
@@ -68,10 +68,9 @@ gunicorn = "*"
 gxformat2 = "*"
 h5grove = ">=1.2.1"
 h5py = "*"
-importlib-metadata = "<5"  # Work around https://github.com/celery/kombu/issues/1600
-importlib-resources = "*"
+importlib-resources = { version = "*", python = "<3.9" }  # for importlib.{abc.Traversable,resources.{files, Package}}
 isa-rwval = ">=0.10.10"
-kombu = "*"
+kombu = ">=5.3"  # for importlib-metadata fix
 lagom = "*"
 Mako = "*"
 Markdown = "*"
@@ -93,9 +92,9 @@ pydantic = {version = "<2", extras = ["email"]}
 PyJWT = "*"
 pykwalify = "*"
 pylibmagic = "*"
-pyparsing = "<3.1"  # Needed by matplotlib on Python >=3.8 https://github.com/matplotlib/matplotlib/issues/26152
+pyparsing = "*"
 pysam = ">=0.21"  # for Python 3.11 support on macOS
-python = ">=3.7,<3.12"  # Keep maximum version strict to appease numpy and scipy
+python = ">=3.8,<3.12"  # Keep maximum version strict to appease numpy and scipy
 python-dateutil = "*"
 python-magic = "*"
 python-multipart = "*"  # required to support form parsing in FastAPI/Starlette
@@ -155,13 +154,14 @@ pytest-postgresql = "!=3.0.0"  # https://github.com/ClearcodeHQ/pytest-postgresq
 pytest-shard = "*"
 responses = "*"
 selenium = "*"
+urllib3 = "<2"  # Necessary for poetry for resolve deps for Python 3.8+
 seletools = "*"
 Sphinx = ">=4.0"  # support docutils 0.17
 sphinx-rtd-theme = ">=1.0.0,<2"  # https://github.com/python-poetry/poetry/issues/8194
 statsd = "*"
 testfixtures = "*"
 tuspy = "*"
-twill = "*"
+twill = ">=3.2.1"  # https://github.com/twill-tools/twill/issues/18
 watchdog = "*"
 
 [tool.poetry.group.typecheck.dependencies]
@@ -184,7 +184,7 @@ types-six = "*"
 # Enable: pycodestyle errors (E), Pyflakes (F), flake8-bugbear (B),
 # flake8-logging-format (G) and pyupgrade (UP)
 select = ["E", "F", "B", "G", "UP"]
-target-version = "py37"
+target-version = "py38"
 # Exceptions:
 # B008 Do not perform function calls in argument defaults (for FastAPI Depends and Body)
 # B9 flake8-bugbear opinionated warnings
diff --git a/scripts/apply_tags.py b/scripts/apply_tags.py
index 32de6df53e00..fabf9470eebf 100644
--- a/scripts/apply_tags.py
+++ b/scripts/apply_tags.py
@@ -136,9 +136,8 @@ def propagate_tags(self, history, current_history_id, parent_datasets_ids, datas
         self_tags = self.collect_hash_tags(own_tags[dataset_id])
         # find unique tags from all parents
         all_tags_set = set(all_tags)
-        self_tags_set = set(self_tags)
         # update tags if there are new tags from parents
-        if all_tags_set != self_tags_set:
+        if all_tags_set != (self_tags_set := set(self_tags)):
             if not all_tags_set.issubset(self_tags_set):
                 # append the tags of the child itself
                 all_tags.extend(self_tags)
diff --git a/scripts/check_model.py b/scripts/check_model.py
index 3f411963270a..d1661df4975e 100644
--- a/scripts/check_model.py
+++ b/scripts/check_model.py
@@ -53,8 +53,7 @@ def load_indexes(metadata):
 
     all_indexes = set(mapping_indexes.keys()) | set(tsi_mapping_indexes.keys())
 
-    missing_indexes = all_indexes - set(indexes_in_db.keys())
-    if missing_indexes:
+    if missing_indexes := all_indexes - set(indexes_in_db.keys()):
         return [(mapping_indexes[index], index.table, index.column_names) for index in missing_indexes]
 
 
diff --git a/scripts/check_python.py b/scripts/check_python.py
index 99fd1c9fcbe8..62a8cfbfa59e 100644
--- a/scripts/check_python.py
+++ b/scripts/check_python.py
@@ -7,7 +7,7 @@
 
 
 def check_python():
-    if sys.version_info[:2] >= (3, 7):  # noqa: UP036
+    if sys.version_info[:2] >= (3, 8):  # noqa: UP036
         # supported
         return
     else:
@@ -15,7 +15,7 @@ def check_python():
         msg = (
             """\
 ERROR: Your Python version is: %s
-Galaxy is currently supported on Python >=3.7 .
+Galaxy is currently supported on Python >=3.8 .
 To run Galaxy, please install a supported Python version.
 If a supported version is already installed but is not your default,
 https://docs.galaxyproject.org/en/latest/admin/python.html contains instructions
diff --git a/scripts/common_startup.sh b/scripts/common_startup.sh
index 720f64a79110..e1fa46fdbac6 100755
--- a/scripts/common_startup.sh
+++ b/scripts/common_startup.sh
@@ -14,7 +14,7 @@ SKIP_NODE=${GALAXY_SKIP_NODE:-0}
 INSTALL_PREBUILT_CLIENT=${GALAXY_INSTALL_PREBUILT_CLIENT:-0}
 NODE_VERSION=${GALAXY_NODE_VERSION:-"$(cat client/.node_version)"}
 : "${YARN_INSTALL_OPTS:=--network-timeout 300000 --check-files}"
-: "${GALAXY_CONDA_PYTHON_VERSION:=3.7}"
+: "${GALAXY_CONDA_PYTHON_VERSION:=3.8}"
 
 for arg in "$@"; do
     if [ "$arg" = "--skip-venv" ]; then
@@ -41,7 +41,7 @@ RMFILES="
     lib/pkg_resources.pyc
 "
 
-MIN_PYTHON_VERSION=3.7
+MIN_PYTHON_VERSION=3.8
 MIN_PIP_VERSION=20.3
 
 # return true if $1 is in $2 else false
diff --git a/test/integration/test_celery_user_rate_limit.py b/test/integration/test_celery_user_rate_limit.py
index 5d58d02f9025..ac69062dfda1 100644
--- a/test/integration/test_celery_user_rate_limit.py
+++ b/test/integration/test_celery_user_rate_limit.py
@@ -24,7 +24,7 @@ def mock_user_id_task(task_user_id: int):
     return task_user_id
 
 
-@lru_cache()
+@lru_cache
 def sqlite_url():
     path = tempfile.NamedTemporaryFile().name
     dburl = f"sqlite:///{path}"
@@ -32,7 +32,7 @@ def sqlite_url():
     return dburl
 
 
-@lru_cache()
+@lru_cache
 def setup_users(dburl: str, num_users: int = 2):
     """
     Setup test users in galaxy_user table with user id's starting from 2.
diff --git a/test/integration/test_kubernetes_runner.py b/test/integration/test_kubernetes_runner.py
index 62a353693ea5..728d2a4fbfdd 100644
--- a/test/integration/test_kubernetes_runner.py
+++ b/test/integration/test_kubernetes_runner.py
@@ -4,6 +4,7 @@
 import collections
 import json
 import os
+import shlex
 import string
 import subprocess
 import tempfile
@@ -18,10 +19,7 @@
 from typing_extensions import Literal
 
 from galaxy.tool_util.verify.wait import timeout_type
-from galaxy.util import (
-    shlex_join,
-    unicodify,
-)
+from galaxy.util import unicodify
 from galaxy_test.base.populators import (
     DatasetPopulator,
     DEFAULT_TIMEOUT,
@@ -361,7 +359,7 @@ def get_kubectl_logs(allow_wait: bool = True) -> Optional[str]:
                 if allow_wait and "is waiting to start" in p.stderr:
                     return None
                 raise Exception(
-                    f"Command '{shlex_join(log_cmd)}' failed with exit code: {p.returncode}.\nstdout: {p.stdout}\nstderr: {p.stderr}"
+                    f"Command '{shlex.join(log_cmd)}' failed with exit code: {p.returncode}.\nstdout: {p.stdout}\nstderr: {p.stderr}"
                 )
             return p.stdout
 
diff --git a/test/unit/app/jobs/test_rule_helper.py b/test/unit/app/jobs/test_rule_helper.py
index eaf98dc0741e..1d1197a0dc78 100644
--- a/test/unit/app/jobs/test_rule_helper.py
+++ b/test/unit/app/jobs/test_rule_helper.py
@@ -56,8 +56,7 @@ def test_job_count():
 
 
 def __assert_job_count_is(expected_count, rule_helper, **kwds):
-    actual_count = rule_helper.job_count(**kwds)
-    if expected_count != actual_count:
+    if expected_count != (actual_count := rule_helper.job_count(**kwds)):
         raise AssertionError(f"Expected job count {expected_count}, actual job count {actual_count} for params {kwds}")
 
 
diff --git a/test/unit/app/managers/test_NotificationManager.py b/test/unit/app/managers/test_NotificationManager.py
index 99d493b3ef3b..b644e20e7b10 100644
--- a/test/unit/app/managers/test_NotificationManager.py
+++ b/test/unit/app/managers/test_NotificationManager.py
@@ -95,14 +95,12 @@ def _assert_notification_expected(self, actual_notification: Any, expected_notif
         assert actual_notification.variant == expected_notification["variant"]
         assert actual_notification.category == expected_notification["category"]
 
-        expected_publication_time = expected_notification.get("publication_time")
-        if expected_publication_time:
+        if expected_publication_time := expected_notification.get("publication_time"):
             assert actual_notification.publication_time == expected_publication_time
         else:
             assert actual_notification.publication_time is not None
 
-        expected_expiration_time = expected_notification.get("expiration_time")
-        if expected_expiration_time:
+        if expected_expiration_time := expected_notification.get("expiration_time"):
             assert actual_notification.expiration_time == expected_expiration_time
         else:
             assert actual_notification.expiration_time is not None
@@ -276,7 +274,7 @@ def test_update_user_notifications(self):
         assert user_notification.seen_time is None
         assert user_notification.deleted is False
         request = UserNotificationUpdateRequest(seen=True)
-        self.notification_manager.update_user_notifications(user, set([notification.id]), request)
+        self.notification_manager.update_user_notifications(user, {notification.id}, request)
         user_notification = self.notification_manager.get_user_notification(user, notification.id)
         assert user_notification.seen_time is not None
         assert user_notification.deleted is False
diff --git a/test/unit/data/model/migrations/test_migrations.py b/test/unit/data/model/migrations/test_migrations.py
index 20665cd1fdd5..51cba45265ab 100644
--- a/test/unit/data/model/migrations/test_migrations.py
+++ b/test/unit/data/model/migrations/test_migrations.py
@@ -43,7 +43,10 @@
     drop_existing_database,
     url_factory,
 )
-from galaxy.util.resources import resource_path
+from galaxy.util.resources import (
+    as_file,
+    resource_path,
+)
 
 # Revision numbers from test versions directories
 GXY_REVISION_0 = "62695fac6cc0"  # oldest/base
@@ -1128,10 +1131,13 @@ def db_state6_gxy_state3_tsi_no_sam(url_factory, metadata_state6_gxy_state3_tsi_
 @pytest.fixture(autouse=True)
 def legacy_manage_db(monkeypatch):
     def get_alembic_cfg():
-        path = resource_path("galaxy.model.migrations", "alembic.ini")
-        config = Config(path)
-        path1, path2 = _get_paths_to_version_locations()
-        config.set_main_option("version_locations", f"{path1};{path2}")
+        traversable = resource_path("galaxy.model.migrations", "alembic.ini")
+        with as_file(traversable) as path:
+            config = Config(path)
+            path1, path2 = _get_paths_to_version_locations()
+            config.set_main_option("version_locations", f"{path1};{path2}")
+            # config.set_main_option() calls config.file_config() which memoizes
+            # the temporary file path from the as_file() contextmanager
         return config
 
     monkeypatch.setattr(galaxy.model.migrations.scripts, "get_alembic_cfg", get_alembic_cfg)
diff --git a/test/unit/data/test_galaxy_mapping.py b/test/unit/data/test_galaxy_mapping.py
index c7a2d4eb8366..c22d51f45eae 100644
--- a/test/unit/data/test_galaxy_mapping.py
+++ b/test/unit/data/test_galaxy_mapping.py
@@ -1095,8 +1095,7 @@ def _make_owned(self, security_agent, user, hda):
 
     def _set_permissions(self, security_agent, dataset, permissions):
         # TODO: refactor set_all_dataset_permissions to actually throw an exception :|
-        error = security_agent.set_all_dataset_permissions(dataset, permissions)
-        if error:
+        if error := security_agent.set_all_dataset_permissions(dataset, permissions):
             raise Exception(error)
 
     def new_hda(self, history, **kwds):
diff --git a/test/unit/tool_util/data/test_tool_data_bundles.py b/test/unit/tool_util/data/test_tool_data_bundles.py
index 2e1a1f271740..9c6afddc5735 100644
--- a/test/unit/tool_util/data/test_tool_data_bundles.py
+++ b/test/unit/tool_util/data/test_tool_data_bundles.py
@@ -15,7 +15,10 @@
     galaxy_directory,
     parse_xml,
 )
-from galaxy.util.resources import resource_path
+from galaxy.util.resources import (
+    as_file,
+    resource_path,
+)
 
 TOOLS_DIRECTORY = os.path.abspath(os.path.join(galaxy_directory(), "test/functional/tools/"))
 
@@ -51,8 +54,8 @@ def test_xml_parsing() -> None:
 
 
 def test_parsing_manual() -> None:
-    path = resource_path(__package__, "example_data_managers/manual.xml")
-    tree = parse_xml(path)
+    with as_file(resource_path(__package__, "example_data_managers/manual.xml")) as path:
+        tree = parse_xml(path)
     data_managers_el = tree.getroot()
     data_manager_el = data_managers_el.find("data_manager")
     description = convert_data_tables_xml(data_manager_el)
@@ -61,8 +64,8 @@ def test_parsing_manual() -> None:
 
 
 def test_parsing_mothur() -> None:
-    path = resource_path(__package__, "example_data_managers/mothur.xml")
-    tree = parse_xml(path)
+    with as_file(resource_path(__package__, "example_data_managers/mothur.xml")) as path:
+        tree = parse_xml(path)
     data_managers_el = tree.getroot()
     data_manager_el = data_managers_el.find("data_manager")
     description = convert_data_tables_xml(data_manager_el)
diff --git a/tox.ini b/tox.ini
index 2bde302555f9..c68144f12885 100644
--- a/tox.ini
+++ b/tox.ini
@@ -39,6 +39,8 @@ passenv =
     GALAXY_CONFIG_OVERRIDE_DATABASE_CONNECTION
     APP_WEBSERVER
     TERM
+    TEST_PYTHON
+    TZ
 setenv =
     coverage: GALAXY_TEST_COVERAGE=1
     first_startup: GALAXY_CONFIG_DATABASE_AUTO_MIGRATE=true