diff --git a/conda/meta.yaml b/conda/meta.yaml index f57c9ea..f99eba6 100644 --- a/conda/meta.yaml +++ b/conda/meta.yaml @@ -23,6 +23,7 @@ requirements: - hdf5 - mpfr - libxml2 + - unzip test: commands: diff --git a/galaxy/tools/mentalist_call/mentalist_call.xml b/galaxy/tools/mentalist_call/mentalist_call.xml index db2327e..e3941f1 100644 --- a/galaxy/tools/mentalist_call/mentalist_call.xml +++ b/galaxy/tools/mentalist_call/mentalist_call.xml @@ -15,9 +15,9 @@ $output_votes $output_special #if $input_type.sPaired == "paired": - -s $input_type.pInput1.name $input_type.pInput1 $input_type.pInput2 + -1 $input_type.pInput1 -2 $input_type.pInput2 #elif $input_type.sPaired == "collections": - -s $input_type.fastq_collection.name $input_type.fastq_collection.forward $input_type.fastq_collection.reverse + -1 $input_type.fastq_collection.forward -2 $input_type.fastq_collection.reverse #end if ]]> @@ -45,13 +45,12 @@ - + - diff --git a/src/mlst_download_functions.jl b/src/mlst_download_functions.jl index 55aea58..41ccdfa 100644 --- a/src/mlst_download_functions.jl +++ b/src/mlst_download_functions.jl @@ -181,50 +181,48 @@ function download_enterobase_scheme(scheme, s_type, output_dir, overwrite=false) return loci_files end -function download_cgmlst_scheme(target_id, output_dir, overwrite=false) +function download_cgmlst_scheme(target_id, output_dir) id = _find_cgmlst_id(target_id) if id == nothing Lumberjack.warn("Id/species ($target_id) not found!") exit(-1) end info("Downloading cgMLST scheme ...") - gzip_alleles = _download_to_folder("http://www.cgmlst.org/ncs/schema/$id/alleles",output_dir) - # unzip file to one FASTA per locus: - loci_files = String[] - current_fasta_fh = nothing + scheme_zip_file = _download_to_folder("http://www.cgmlst.org/ncs/schema/$id/alleles", output_dir) + locus_files = String[] locus = "" - fh = GZip.open(gzip_alleles) - info("Unzipping cgMLST scheme into individual FASTA files for each loci ...") - n_locus = 0 - for l in eachline(fh) - # skip empty lines or "=" - if length(strip(l)) == 0 || startswith(l, "=") - continue - end - if startswith(l,"#") - # print to show progress: - if n_locus % 200 == 0 + info("Unzipping cgMLST scheme into individual FASTA files for each locus ...") + scheme_dirname = dirname(scheme_zip_file) + run(`unzip -oq $scheme_zip_file -d $scheme_dirname/tmp`) + rm(scheme_zip_file) + scheme_files = readdir(joinpath(scheme_dirname, "tmp")) + + for scheme_file in scheme_files + # print to show progress: + if length(locus_files) % 200 == 0 print(".") + end + scheme_file_path = joinpath(scheme_dirname, scheme_file) + push!(locus_files, scheme_file_path) + # get locus ID from filename + locus = split(scheme_file, ".")[1] + fh = open(scheme_file_path, "w") + for l in eachline(joinpath(scheme_dirname, "tmp", scheme_file)) + if length(strip(l)) == 0 + continue end - n_locus += 1 - locus = strip(l[2:end]) # remove the starting '#' - if current_fasta_fh != nothing - close(current_fasta_fh) - end - fasta = joinpath(output_dir, "$locus.fa") - push!(loci_files, fasta) - current_fasta_fh = open(fasta, "w") - elseif startswith(l,">") - # substitute the number only to locus_number - id = strip(l[2:end]) - write(current_fasta_fh, ">$(locus)_$id\n") - else # - if current_fasta_fh != nothing - write(current_fasta_fh, "$l\n") + if l[1] == '>' + l = '>' * locus * "_" * l[2:end] * "\n" + write(fh, l) + else + write(fh, l * "\n") end end + close(fh) end println() - info("$n_locus loci found.") - return loci_files + total_loci = length(locus_files) + info("$total_loci loci found.") + rm(joinpath(scheme_dirname, "tmp"), recursive=true) + return locus_files end diff --git a/test/test_data/l_pneumophila_cgmlst_allele_filenames.txt b/test/test_data/l_pneumophila_cgmlst_allele_filenames.txt index 9f3e4ae..6b4364c 100644 --- a/test/test_data/l_pneumophila_cgmlst_allele_filenames.txt +++ b/test/test_data/l_pneumophila_cgmlst_allele_filenames.txt @@ -1,1521 +1,1521 @@ 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