Main tool: stxtyper
Additional tools:
- ncbi-blast+ 2.12.0
Basic information on how to use this tool:
- executable:
stxtyper
- help:
stxtyper --help
- version:
stxtyper --version
- description: Detects and types Shiga toxin genes in nucleotide sequences
Full documentation: https://github.com/ncbi/stxtyper
Note: This software is now incorporated into NCBI's AMRFinderPlus software as of version 4.0.3. Stxtyper will run under-the-hood of AMRFinderPlus when using the amrfinder --organism Escherichia
option.
# stxtyper accepts both gzipped and uncompressed FASTA files
stxtyper -n GCA_012224845.2_ASM1222484v2_genomic.fna.gz -o stxtyper-results.tsv
$ column -t test-result.tsv
#target_contig stx_type operon identity target_start target_stop target_strand A_reference A_reference_subtype A_identity A_coverage B_reference B_reference_subtype B_identity B_coverage
CP113091.1 stx2o COMPLETE 100.00 2085533 2086768 + WAK52085.1 stxA2o 100.00 100.00 QZL10983.1 stxB2o 100.00 100.00