Unable to use constraints files in a simple minimization procedure

[oroscow]

I'm trying to replicate this example. Here's a minimal code example:

Directory:

- 3hon.pdb
- 3hon.pdb_0001.pdb
- cstfile.cst
- min_flag

Flag file:

-s 3hon.pdb
-run:min_tolerance 0.001
-constraints:cst_file cstfile
-score:weights ref2015_cst
-out:suffix _minwithcsts

Constraints file:

AtomPair CA 111 CA 249 CONSTANTFUNC 5
Angle OE2 363 NH2 76 CZ 76 CONSTANTFUNC 6
Dihedral CG 221 ND2 221 OE1 165 CD 165 CONSTANTFUNC 7
AtomPair CA 445 OH 445 CONSTANTFUNC 9
Dihedral CG 25 ND2 25 CA 25 N 25  CONSTANTFUNC 8

I got the contents of my constraints file here.

Rosetta command:

minimize @min_flag

Crash log (abbreviated):

[FILE]: src/core/scoring/constraints/ConstraintIO.cc
[LINE]: 454
[START_MESSAGE]
[ ERROR ] UtilityExitException
ERROR: [ERROR] Unable to open constraints file: cstfile

[roccomoretti]

Rosetta doesn't (necessarily) add the .cst extension to constraint files. You'll need to use -constraints:cst_file cstfile.cst for Rosetta to properly recognize the file cstfile.cst in the working directory.

[oroscow]

As usual, a simple solution; thanks so much!