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paneleditor_GUI() $PnR value truncated #51

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lchan8 opened this issue Feb 24, 2023 · 2 comments
Open

paneleditor_GUI() $PnR value truncated #51

lchan8 opened this issue Feb 24, 2023 · 2 comments

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@lchan8
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lchan8 commented Feb 24, 2023

Hello,

I viewed my files in FlowJo pre- and post-Premessa panel editing and noticed a discrepancy. Each marker's plot got altered post-panel editing. Below are the plots for the same sample, before and after panel editing. The channels are correctly renamed.
image
image

I noticed that I am getting the following warnings when I run paneleditor_GUI(). How do I fix $PnR?
Warning in readFCSdata(con, offsets, txt, transformation, which.lines, scale, :
Some data values of 'Yb171Di' channel exceed its $PnR value 1 and will be truncated!
To avoid truncation, either fix $PnR before generating FCS or set 'truncate_max_range = FALSE'
Warning in readFCSdata(con, offsets, txt, transformation, which.lines, scale, :
Some data values of 'beadDist' channel exceed its $PnR value 1 and will be truncated!
To avoid truncation, either fix $PnR before generating FCS or set 'truncate_max_range = FALSE'

With paneleditor_GUI(), there is no option to set 'truncate_max_range = FALSE'. My flowCore version is ‘2.10.0’

@lchan8 lchan8 changed the title Discrepancy in FlowJo plots pre- and post-Premessa panel editing paneleditor_GUI() $PnR value truncated Feb 27, 2023
@lchan8
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lchan8 commented Mar 2, 2023

The FCS files are not truncated if I did panel editing prior to gating in FlowJo then exporting. Could be a FlowJo bug?

@SamGG
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SamGG commented Mar 2, 2023

Solution to test posted at http://cytoforum.stanford.edu/viewtopic.php?f=4&t=3621

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