Debarcoding issue with a 7 choose 3 scheme

[simonuoft]

Hey,

I'm trying to debarcode a file that have been barcoded with a 7 choose 3 scheme.
When debarcoding, the package debarcode based on the combinations that have been described in the key, however the combination are assigned to wrong samples.

Here is an example of debarcoded file : the correct barcoding scheme for this sample(sample 38) should be 0011010 and based on the plot it's clearly 0110100.

It look like there a shift somwhere but can't figure out where.

So i have few question to try to understand the origin/fix the problem:

1. The barcodes that I'm using don't all have the same intensity can that be problematic to debarcode ?
2. It look like the normalization doesn't change anyhting (left plot and right one are the same) is that normal ?

Thanks in advance