How to set a modification not C-term?

[a995752924]

Hello,

When using the Fragpipe, I have a problem with the modification setting. How to set a modification not C-term, like this in Spectronaut?

Thank you!

[fcyu]

Current version does not support it.

Best,

Fengchao

[anesvi]

Could be a useful option to enable. I assume you want to look for PTMs on K that should prevent trypsin from cleaving next to it? You could also just apply an extra filter at the end?

[a995752924]

Could be a useful option to enable. I assume you want to look for PTMs on K that should prevent trypsin from cleaving next to it? You could also just apply an extra filter at the end?

Thanks. I assume that PTMs on K prevent trypsin from cleaving next to it. Can I just remove the precursors with PTMs on C-term in the "report.tsv"? Will this influence the quantification of the PTM sites?