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Summarization of the technical replicate #63

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Laurems4ms opened this issue Dec 2, 2024 · 0 comments
Open

Summarization of the technical replicate #63

Laurems4ms opened this issue Dec 2, 2024 · 0 comments

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@Laurems4ms
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Hey the FragPipe Team !
Thank you very much for this tool, it is very easy to use !
I am just wondering if i am doing well, I have two strange thing for me...
I do an DIA analysis, with median centred normalization, no imputation, Min percentage of non-missing values in at least one condition 50%. I have 3 conditions some with 1, or 2 or 3 or 4 or 5 technical replicate and other without.
The matrix that I obtained after the analysis with FragPipeAnalyst are always with technical replicates separated, I means there is no summarization of the technical replicate in the Normalized_matrix, Filtered_matrix or full_dataset.
For the limma GC it is also like that ?
Is it normal ? Or it is an issu with my experiment_annotation file ?
image

Also the normalized_matrix and the full_dataset show positive and negative numbers between -5 and 9...
image

Have you any advice ? Thank you very much for your help !

Laure.

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