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Issue with ligation barcode length in code #5

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Gaura opened this issue Jul 29, 2021 · 0 comments
Open

Issue with ligation barcode length in code #5

Gaura opened this issue Jul 29, 2021 · 0 comments

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@Gaura
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Gaura commented Jul 29, 2021

Given that the ligation barcode can be either 9 or 10 bp long. Wouldn't considering only 10bp length of the ligation barcode ignore all the 9bp barcode reads?

sci-RNA-seq3_pipeline/script_folder/UMI_barcode_attach_gzipped_with_dic.py

Line 44 in f8f3497

tmp_lig = line1[0:10]

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@Gaura