From fb8c8eccbbea8f339159e111022825849372144d Mon Sep 17 00:00:00 2001 From: Ziv Yaniv Date: Tue, 31 Oct 2023 13:11:00 -0400 Subject: [PATCH] Correct catalog numbers. Antigen retrieval conditions listed incorrect catalog numbers for ER1. AF9961 corrected to AR9961. resolves #87 --- data/reagent_resources.csv | 6 +++--- docs/supporting_material/CD21_PE/0000-0003-4379-8967.md | 2 +- .../CFTR_Unconjugated/0000-0002-2950-2683.md | 2 +- .../PIGR_Unconjugated/0000-0002-2950-2683.md | 2 +- 4 files changed, 6 insertions(+), 6 deletions(-) diff --git a/data/reagent_resources.csv b/data/reagent_resources.csv index 6e83d11..559bf7d 100644 --- a/data/reagent_resources.csv +++ b/data/reagent_resources.csv @@ -620,11 +620,11 @@ Q9UKI9,Primary Antibody,POU2F3,Human,Rabbit,IgG,Polyclonal,Novus Biologicals,NBP Q12912,Primary Antibody,LRMP,Human,Rabbit,IgG,EPR17852,Abcam,ab202418,Unconjugated,NA,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q76IQ4,Primary Antibody,Ghrelin,Human,Mouse,IgG2b,891240,R&D Systems,MAB8149,Unconjugated,NA,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Jejunum,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P13569,Primary Antibody,CFTR,Human,Mouse,IgM,CF3,Thermo Fisher Scientific,MA1-935,Unconjugated,AB_2081230,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Pancreas,NA,0.3% Triton-X-100,NA,NA,No,0000-0002-2950-2683,NA,0000-0002-2950-2683 -P13569,Primary Antibody,CFTR,Human,Mouse,IgM,CF3,Thermo Fisher Scientific,MA1-935,Unconjugated,AB_2081230,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AF9961) using the Leica Bond,NA,Yes,0000-0002-2950-2683,NA,0000-0002-2950-2683 +P13569,Primary Antibody,CFTR,Human,Mouse,IgM,CF3,Thermo Fisher Scientific,MA1-935,Unconjugated,AB_2081230,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond,NA,Yes,0000-0002-2950-2683,NA,0000-0002-2950-2683 P13569,Primary Antibody,CFTR,Human,Mouse,IgG1,M3A7,Thermo Fisher Scientific,MA5-11768,Unconjugated,AB_10983660,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Pancreas,NA,0.3% Triton-X-100,NA,NA,No,0000-0002-2950-2683,NA,0000-0002-2950-2683 P01833,Primary Antibody,PIGR,Human,Rabbit,IgG,Polyclonal,Abcam,ab96196,Unconjugated,AB_10677612,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Pancreas,NA,0.3% Triton-X-100,NA,NA,No,0000-0002-2950-2683,NA,0000-0002-2950-2683 P01833,Primary Antibody,PIGR,Human,Rat,IgG1,7C1,Abcam,ab170321,Unconjugated,NA,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Jejunum,NA,0.3% Triton-X-100,NA,NA,No,0000-0002-2950-2683,NA,0000-0002-2950-2683 -P01833,Primary Antibody,PIGR,Human,Rat,IgG1,7C1,Abcam,ab170321,Unconjugated,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AF9961) using the Leica Bond,NA,Yes,0000-0002-2950-2683,NA,0000-0002-2950-2683 +P01833,Primary Antibody,PIGR,Human,Rat,IgG1,7C1,Abcam,ab170321,Unconjugated,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond,NA,Yes,0000-0002-2950-2683,NA,0000-0002-2950-2683 P01833,Primary Antibody,PIGR,Human,Rabbit,IgG,Polyclonal,Thermo Fisher Scientific,PA5-35340,Unconjugated,AB_2552650,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,NA,No,0000-0002-2950-2683,NA,0000-0002-2950-2683 P07476,Primary Antibody,Involucrin,Human,Mouse,IgG1,SY5,Santa Cruz Biotechnology,sc-21748 AF488,AF488,AB_2128119,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Skin,NA,0.3% Triton-X-100,NA,NA,No,0000-0002-2950-2683,NA,0000-0002-2950-2683 P07476,Primary Antibody,Involucrin,Human,Rabbit,IgG,Polyclonal,Abcam,ab53112,Unconjugated,AB_880813,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0002-2950-2683,NA,0000-0002-2950-2683 @@ -966,7 +966,7 @@ P62736,Primary Antibody,Alpha smooth muscle actin,Human,Mouse,IgG2a,1A4,Thermo F P62736,Primary Antibody,Alpha smooth muscle actin,Human,Mouse,IgG2a,1A4,Thermo Fisher Scientific,50-9760-82,eF660,AB_2574362,Stock,IBEX2D Manual,FFPE,Lung,Infected,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0003-3697-5726,NA,0000-0003-3697-5726 NA,Primary Antibody,Cytokeratin (Pan),Human,Mouse,IgG1,AE1/AE3,Thermo Fisher Scientific,50-9003-82,eF660,AB_2574301,Stock,IBEX2D Manual,FFPE,Lung,Infected,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0003-3697-5726,NA,0000-0003-3697-5726 P08670,Primary Antibody,Vimentin,Human,Mouse,IgG2a,O91D3,BioLegend,677804,AF594,AB_2566179,Stock,IBEX2D Manual,FFPE,Lung,Infected,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 for more than 120 minutes,Yes,0000-0003-3697-5726,NA,0000-0003-3697-5726 -P20023,Primary Antibody,CD21,Human,Rabbit,IgG,SP186,Abcam,ab306325,PE,NA,Stock,Cell DIVE-IBEX,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AF9961) using the Leica Bond,1 mg/ml LiBH4 15 minutes,No,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P20023,Primary Antibody,CD21,Human,Rabbit,IgG,SP186,Abcam,ab306325,PE,NA,Stock,Cell DIVE-IBEX,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond,1 mg/ml LiBH4 15 minutes,No,0000-0003-4379-8967,NA,0000-0003-4379-8967 P56199,Primary Antibody,CD49a,Human,Mouse,IgG1,CL7207,Abcam,ab243032,Unconjugated,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P56199,Primary Antibody,CD49a,Human,Mouse,IgG1,CL7207,Abcam,ab243032,Unconjugated,NA,Stock,Cell DIVE-IBEX,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 NA,Secondary Antibody,Fluorescein/Oregon Green,Human,Rabbit,IgG,Polyclonal,Thermo Fisher Scientific,A-11090,AF488,AB_221562,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 diff --git a/docs/supporting_material/CD21_PE/0000-0003-4379-8967.md b/docs/supporting_material/CD21_PE/0000-0003-4379-8967.md index 48123bb..04ffd19 100644 --- a/docs/supporting_material/CD21_PE/0000-0003-4379-8967.md +++ b/docs/supporting_material/CD21_PE/0000-0003-4379-8967.md @@ -8,7 +8,7 @@ layout: default |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|:-----------------|:------------|:-----------|:---------------|:---------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------------------------------------------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:--------------------|:------------| | P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354903 | PE | AB_2561406 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications), [1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | | P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) | -| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | No | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) | +| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | No | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) | # Publications diff --git a/docs/supporting_material/CFTR_Unconjugated/0000-0002-2950-2683.md b/docs/supporting_material/CFTR_Unconjugated/0000-0002-2950-2683.md index 4fc41f1..f73b16e 100644 --- a/docs/supporting_material/CFTR_Unconjugated/0000-0002-2950-2683.md +++ b/docs/supporting_material/CFTR_Unconjugated/0000-0002-2950-2683.md @@ -7,7 +7,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:-------------------------|:-----------------|:-------------|:------------|:---------------|:-----------------------|:----------------------------------|:----------------|:---------------|:------------------|:------------------------------------------------------|:------------------------------|:------------|:--------------------|:-----------|:--------------------|:------------| | P13569 | Primary Antibody | CFTR | Human | Mouse | IgM | CF3 | Thermo Fisher Scientific | MA1-935 | Unconjugated | AB_2081230 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [1](#notes) | -| P13569 | Primary Antibody | CFTR | Human | Mouse | IgM | CF3 | Thermo Fisher Scientific | MA1-935 | Unconjugated | AB_2081230 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) using the Leica Bond | NA | Yes | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [2](#notes) | +| P13569 | Primary Antibody | CFTR | Human | Mouse | IgM | CF3 | Thermo Fisher Scientific | MA1-935 | Unconjugated | AB_2081230 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [2](#notes) | | P13569 | Primary Antibody | CFTR | Human | Mouse | IgG1 | M3A7 | Thermo Fisher Scientific | MA5-11768 | Unconjugated | AB_10983660 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [3](#notes) | # Publications diff --git a/docs/supporting_material/PIGR_Unconjugated/0000-0002-2950-2683.md b/docs/supporting_material/PIGR_Unconjugated/0000-0002-2950-2683.md index 61336f6..ad3dd93 100644 --- a/docs/supporting_material/PIGR_Unconjugated/0000-0002-2950-2683.md +++ b/docs/supporting_material/PIGR_Unconjugated/0000-0002-2950-2683.md @@ -8,7 +8,7 @@ layout: default |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:-------------------------|:-----------------|:-------------|:------------|:---------------|:-----------------------|:----------------------------------|:----------------|:---------------|:------------------|:------------------------------------------------------|:------------------------------|:------------|:--------------------|:-----------|:--------------------|:------------| | P01833 | Primary Antibody | PIGR | Human | Rabbit | IgG | Polyclonal | Abcam | ab96196 | Unconjugated | AB_10677612 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [1](#notes) | | P01833 | Primary Antibody | PIGR | Human | Rat | IgG1 | 7C1 | Abcam | ab170321 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [2](#notes) | -| P01833 | Primary Antibody | PIGR | Human | Rat | IgG1 | 7C1 | Abcam | ab170321 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) using the Leica Bond | NA | Yes | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [3](#notes) | +| P01833 | Primary Antibody | PIGR | Human | Rat | IgG1 | 7C1 | Abcam | ab170321 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [3](#notes) | | P01833 | Primary Antibody | PIGR | Human | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | PA5-35340 | Unconjugated | AB_2552650 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | NA | [0000-0002-2950-2683](https://orcid.org/0000-0002-2950-2683) | [4](#notes) | # Publications