diff --git a/data/image_resources.csv b/data/image_resources.csv index 9abf676..509f2cf 100644 --- a/data/image_resources.csv +++ b/data/image_resources.csv @@ -164,3 +164,22 @@ CD45_PE/Human_Tonsil_FFPE_Hoechst_blue_CD45_PE_green.jpg,"Human tonsil: Hoechst CD8_AF647/Human_Tonsil_FFPE_Hoechst_blue_CD8_red.jpg,"Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD8 (red, BioLegend cat. no. 372906)",e2a8ea56916b2337125ea81c18050771,"['CD8_AF647/0009-0006-9784-2694.md']" CTLA4_AF647/Human_Tonsil_FFPE_Hoechst_blue_CTLA4_red.jpg,"Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CTLA4 (red, Abcam cat. no. ab270127)",0319ef9d4863816c1d28cfa5e3ec8258,"['CTLA4_AF647/0009-0006-9784-2694.md']" PD-1_AF555/Human_Tonsil_FFPE_Hoechst_blue_PD1_green.jpg,"Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and PD-1 (green, Abcam cat. no. ab275126)",32a9ea3243d06baf17be375554c5a9d7,"['PD-1_AF555/0009-0006-9784-2694.md']" +CD23_AF647/CD23_AF647_Grey_Mouse_LN_2_Large.jpg,"Mouse (naive) lymph node: CD23 (grey, catalog number 101611)",3c6684993361eb1aa6a61f6054b2fd2b,['CD23_AF647/0000-0002-8909-5039'] +CD23_AF647/CD23_AF647_Grey_Mouse_LN_1_Large.jpg,"Mouse (immunized) lymph node: CD23 (grey, catalog number 101611)",1e9203e92c26bf6b151ad4872c24a7c7,['CD23_AF647/0000-0002-8909-5039'] +CD35_BV510/CD35_BV510_Green_DAPI_Blue_Mouse_LN.jpg,"Mouse (immunized) lymph node: CD35 (green, catalog number 740132) and DAPI (blue, catalog number 62248)",284a4ff657463f54944c9bc42c6cebec,['CD35_BV510/0000-0002-1461-0999'] +Alpha_smooth_muscle_actin_AF488/aSMA_AF488_Grey_Mouse_SI.jpg,"Mouse small intestine: alpha smooth muscle actin (grey, catalogue number 53-9760-82)",51a470bd7b549f77a5d8616442527b67,['Alpha_smooth_muscle_actin_AF488/0000-0002-1461-0999'] +B220_AF647/B220_AF647_Grey_Mouse_LN.jpg,"Mouse lymph node: B220 (grey, catalog number 103226)",594441a0105afe2c0f2d0f078e1301f2,['B220_AF647/0000-0002-1461-0999'] +CD11b_PE/CD11b_PE_Grey_Mouse_LN.jpg,"Mouse lymph node: CD11b (grey, catalog number 101217)",fb45af754793db4a66ae930241c7613d,['CD11b_PE/0000-0002-8909-5039'] +CD11c_AF488/CD11c_AF488_Grey_Mouse_LN.jpg,"Mouse lymph node: CD11c (grey, catalog number MCD11c20)",ee6003913fbc784cae4f0881658c1ff3,['CD11c_AF488/0000-0002-1461-0999'] +CD200_BV421/CD200_BV421_Grey_Mouse_LN.jpg,"Mouse lymph node: CD11c (grey, catalog number 565547)",354c6e657d05a678e0406158f51b294d,['CD200_BV421/0000-0002-1461-0999'] +CD3_AF488/CD3_488_Grey_Mouse_LN.jpg,"Mouse lymph node: CD3 (grey, catalog number 100210)",87f4c2106f8e91275784e64caca088b7,['CD3_AF488/0000-0002-1461-0999'] +CD31_AF594/CD31_AF594_Grey_Mouse_LN.jpg,"Mouse lymph node: CD31 (grey, catalog number 102520)",e96c4e0587f845e94e34f8e74d72cdd6,['CD31_AF594/0000-0002-1461-0999'] +CD4_BV421/CD4_BV421_Grey_Mouse_LN_large.jpg,"Mouse lymph node: CD4 (grey, catalog number 100449)",f7e2ff75a88dc016e7b436dc8e120641,['CD4_BV421/0000-0002-1461-0999'] +CD4_BV510/CD4_BV510_Grey_Mouse_LN.jpg,"Mouse lymph node: CD4 (grey, catalog number 100443)",ffaa812ed72823857aed78d747ba0306,['CD4_BV510/0000-0002-1461-0999'] +CD45.1_AF488/CD45.1_AF488_Magenta_CD138_AF647_Yellow_Mouse_LN.jpg,"Mouse (immunized) lymph node: CD45.1 (yellow, catalog number 110718) and CD138 (magenta, catalog number 142526)",40018e55494cb00bb37f8f5f1210c83c,['CD45.1_AF488/0000-0002-8909-5039'] +CD45_AF594/CD45_AF594_Grey_Mouse_LN.jpg,"Mouse lymph node: CD45 (grey, catalog number 103144)",e8e0fabc8fcf9a6d0dd6d9543a535a89,['CD45_AF594/0000-0002-1461-0999'] +CD64_AF647/CD64_AF647_Grey_Mouse_LN.jpg,"Mouse lymph node: CD64 (grey, catalog number 139322)",0a8e934ad6cc1de5091f8710a9ed41a9,['CD64_AF647/0000-0002-1461-0999'] +CD81_PE/CD81_PE_Grey_Mouse_LN.jpg,"Mouse lymph node: CD3 (grey, catalog number 104905)",aa8dfb29dc0bf9db5e1cc5dfbc24741f,['CD81_PE/0000-0002-8909-5039'] +IgD_BV510/IgD_BV510_Green_B220_AF647_Blue_Mouse_LN.jpg,"Mouse lymph node: IgD (green, catalog number 405723) and B220 (blue, catalog number 103226)",cb40bddb73e073c692e25d967d92c70f,['IgD_BV510/0000-0002-1461-0999'] +Ki-67_AF488/Ki67_AF488_Grey_Mouse_LN.jpg,"Mouse lymph node: Ki67 (grey, catalog number 558616)",5638e7e89b73d31bc55b1dece18752fc,['Ki-67_AF488/0000-0002-1461-0999'] +Pax5_AF647/Pax5_AF647_Margenta_CD3_AF488_Cyan_Mouse_LN.jpg,"Mouse lymph node: Pax5 (magenta, catalog number 649704) and CD3 (cyan, catalog number 100210)",74a7a3125d784c1bdcc0f84fad9f96dc,['Pax5_AF647/0000-0002-1461-0999'] diff --git a/data/reagent_resources.csv b/data/reagent_resources.csv index fe05db5..6cc689c 100644 --- a/data/reagent_resources.csv +++ b/data/reagent_resources.csv @@ -286,7 +286,7 @@ Q61830,Primary Antibody,CD206,Mouse,Rat,IgG2a,C068C2,BioLegend,141717,BV421,AB_2 Q61830,Primary Antibody,CD206,Mouse,Rat,IgG2a,C068C2,BioLegend,141717,BV421,AB_2562232,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P62737,Primary Antibody,Alpha smooth muscle actin,Mouse,Mouse,IgG2a,1A4,Thermo Fisher Scientific,53-9760-82,AF488,AB_2574461,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P62737,Primary Antibody,Alpha smooth muscle actin,Mouse,Mouse,IgG2a,1A4,Thermo Fisher Scientific,53-9760-82,AF488,AB_2574461,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lung,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -P62737,Primary Antibody,Alpha smooth muscle actin,Mouse,Mouse,IgG2a,1A4,Thermo Fisher Scientific,53-9760-82,AF488,AB_2574461,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P62737,Primary Antibody,Alpha smooth muscle actin,Mouse,Mouse,IgG2a,1A4,Thermo Fisher Scientific,53-9760-82,AF488,AB_2574461,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967;0000-0002-1461-0999,NA,0000-0003-4379-8967 P15379,Primary Antibody,CD44,Mouse,Rat,IgG2b,IM7,BioLegend,103002 (Unconjugated),AF532,AB_2892768,Custom,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P06800,Primary Antibody,CD45,Mouse,Rat,IgG2b,30-F11,BioLegend,103128,AF700,AB_493715,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 NA,Primary Antibody,Cytokeratin,Mouse,Mouse,IgG1,C-11,BioLegend,628604,AF647,AB_2563652,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 @@ -330,23 +330,23 @@ P24788,Primary Antibody,CD11b,Mouse,Rat,IgG2b,M1/70,BioLegend,101217,AF488,AB_38 P15104,Primary Antibody,Glutamine synthetase,Mouse,Rabbit,IgG,Polyclonal,Abcam,ab49873,Unconjugated,AB_880241,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Liver,NA,NA,NA,NA,Yes,0000-0003-1431-1398,NA,0000-0003-1431-1398 P11609,Primary Antibody,CD1d,Mouse,Rat,IgG2b,1B1,BD Biosciences,553846,PE,AB_2073521,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Liver,NA,NA,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-1431-1398,NA,0000-0003-1431-1398 Q02242,Primary Antibody,PD-1,Mouse,Rat,IgG2a,29F.1.A12,BioLegend,135218,BV421,AB_2561447,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967;0000-0003-1118-7432,NA,0000-0003-4379-8967 -NA,Primary Antibody,CD35,Mouse,Rat,IgG2a,8C12,BD Biosciences,740132,BV510,AB_2739889,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967;0000-0003-1118-7432,NA,0000-0003-4379-8967 +NA,Primary Antibody,CD35,Mouse,Rat,IgG2a,8C12,BD Biosciences,740132,BV510,AB_2739889,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967;0000-0003-1118-7432;0000-0002-1461-0999,NA,0000-0003-4379-8967 NA,Primary Antibody,B220,Mouse,Rat,IgG2a,RA3-6B2,BD Biosciences,557669,AF488,AB_396781,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967;0000-0003-1118-7432,NA,0000-0003-4379-8967 P41183,Primary Antibody,BCL6,Mouse,Mouse,IgG1,K112-91,BD Biosciences,561525,AF647,AB_10898007,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -Q9ES57,Primary Antibody,CD200,Mouse,Rat,IgG2a,OX-90,BD Biosciences,565547,BV421,AB_2739289,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +Q9ES57,Primary Antibody,CD200,Mouse,Rat,IgG2a,OX-90,BD Biosciences,565547,BV421,AB_2739289,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967;0000-0002-1461-0999,NA,0000-0003-4379-8967 Q62011,Primary Antibody,gp38,Mouse,Syrian Hamster,IgG,8.1.1,BioLegend,127401,Unconjugated,AB_1089186,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 NA,Secondary Antibody,Hamster IgG (H+L),Mouse,Goat,IgG,Polyclonal,Thermo Fisher Scientific,A-21451,AF647,AB_2535868,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q8VBX4,Primary Antibody,CD207,Mouse,Rat,IgG2a,eBioL31,Thermo Fisher Scientific,12-2075-80,PE,AB_763453,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 NA,Primary Antibody,F4/80,Mouse,Rat,IgG2a,BM8,BioLegend,123132,BV421,AB_11203717,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P06332,Primary Antibody,CD4,Mouse,Rat,IgG2b,GK1.5,BD Biosciences,553730,PE,AB_396634,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 NA,Primary Antibody,TCR gamma delta,Mouse,Armenian Hamster,IgG,GL3,BioLegend,118108,PE,AB_313832,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -P26151,Primary Antibody,CD64,Mouse,Mouse,IgG1,X54-5/7.1,BioLegend,139322,AF647,AB_2566561,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P26151,Primary Antibody,CD64,Mouse,Mouse,IgG1,X54-5/7.1,BioLegend,139322,AF647,AB_2566561,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967;0000-0002-1461-0999,NA,0000-0003-4379-8967 NA,Primary Antibody,CD21,Mouse,Rat,IgG2a,7.E.9,BioLegend,123413,Pacific Blue,AB_2085159,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P29533,Primary Antibody,CD106,Mouse,Rat,IgG2a,429,BioLegend,105710,AF488,AB_493427,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -P20693,Primary Antibody,CD23,Mouse,Rat,IgG2a,B3B4,BioLegend,101611,AF647,AB_493479,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P20693,Primary Antibody,CD23,Mouse,Rat,IgG2a,B3B4,BioLegend,101611,AF647,AB_493479,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967;0000-0002-8909-5039,NA,0000-0003-4379-8967 Q64287,Primary Antibody,IRF4,Mouse,Rat,IgG1,3.E.4,Thermo Fisher Scientific,11-9858-82,FITC,AB_2572538,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 30 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q920G3,Primary Antibody,SiglecF,Mouse,Rat,IgG2a,E50-2440,BD Biosciences,552126,PE,AB_394341,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -Q02650,Primary Antibody,Pax5,Mouse,Rat,IgG2a,1H9,BioLegend,649704,AF647,AB_2562425,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +Q02650,Primary Antibody,Pax5,Mouse,Rat,IgG2a,1H9,BioLegend,649704,AF647,AB_2562425,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967;0000-0002-1461-0999,NA,0000-0003-4379-8967 NA,Primary Antibody,GL7,Mouse,Rat,IgM,GL7,BD Biosciences,561530,PE,AB_10715834,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 NA,Primary Antibody,MHC-II,Mouse,Rat,IgG2b,M5/114.15.2,BioLegend,107631,BV421,AB_10900075,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 NA,Primary Antibody,IgD,Mouse,Rat,IgG2a,11-26c.2a,BioLegend,405718,AF488,AB_10730619,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 @@ -384,23 +384,23 @@ NA,Primary Antibody,B220,Mouse,Rat,IgG2a,RA3-6B2,Thermo Fisher Scientific,42-045 NA,Primary Antibody,B220,Mouse,Rat,IgG2a,RA3-6B2,BioLegend,103232,AF700,AB_493717,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P22646,Primary Antibody,CD3,Mouse,Rat,IgG2b,17A2,BioLegend,100228,BV421,AB_2562553,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P22646,Primary Antibody,CD3,Mouse,Rat,IgG2b,17A2,BioLegend,100234,BV510,AB_2562555,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -P22646,Primary Antibody,CD3,Mouse,Rat,IgG2b,17A2,BioLegend,100210,AF488,AB_389301,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P22646,Primary Antibody,CD3,Mouse,Rat,IgG2b,17A2,BioLegend,100210,AF488,AB_389301,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967;0000-0002-1461-0999,NA,0000-0003-4379-8967 P22646,Primary Antibody,CD3,Mouse,Rat,IgG2b,17A2,BioLegend,100205,PE,AB_312662,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -P06332,Primary Antibody,CD4,Mouse,Rat,IgG2b,GK1.5,BioLegend,100449,BV510,AB_2564587,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P06332,Primary Antibody,CD4,Mouse,Rat,IgG2b,GK1.5,BioLegend,100449,BV510,AB_2564587,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,0000-0002-1461-0999,0000-0003-4379-8967 P06332,Primary Antibody,CD4,Mouse,Rat,IgG2a,RM4-5,BD Biosciences,557667,AF488,AB_396779,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P01731,Primary Antibody,CD8,Mouse,Rat,IgG2a,53-6.7,BioLegend,100752,BV510,AB_2563057,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P01731,Primary Antibody,CD8,Mouse,Rat,IgG2a,53-6.7,BioLegend,100723,AF488,AB_389304,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P01731,Primary Antibody,CD8,Mouse,Rat,IgG2a,53-6.7,BD Biosciences,553032,PE,AB_394571,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P01731,Primary Antibody,CD8,Mouse,Rat,IgG2a,53-6.7,BioLegend,100758,AF594,AB_2563237,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 for more than 120 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P24788,Primary Antibody,CD11b,Mouse,Rat,IgG2b,5C6,Bio-Rad,MCA711PE,PE,AB_323678,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -Q9QXH4,Primary Antibody,CD11c,Mouse,Armenian Hamster,IgG,N418,Thermo Fisher Scientific,MCD11c20,AF488,AB_10373244,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +Q9QXH4,Primary Antibody,CD11c,Mouse,Armenian Hamster,IgG,N418,Thermo Fisher Scientific,MCD11c20,AF488,AB_10373244,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967;0000-0002-1461-0999,NA,0000-0003-4379-8967 Q08481,Primary Antibody,CD31,Mouse,Rat,IgG2a,MEC13.3,BioLegend,102514,AF488,AB_2161031,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P06800,Primary Antibody,CD45,Mouse,Rat,IgG2b,30-F11,BioLegend,103134,BV421,AB_2562559,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P06800,Primary Antibody,CD45,Mouse,Rat,IgG2b,30-F11,BioLegend,103138,BV510,AB_2563061,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P06800,Primary Antibody,CD45,Mouse,Rat,IgG2b,30-F11,BioLegend,103122,AF488,AB_493531,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P06800,Primary Antibody,CD45,Mouse,Rat,IgG2b,30-F11,Thermo Fisher Scientific,58-0451-82,AF532,AB_11218871,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P31996,Primary Antibody,CD68,Mouse,Rat,IgG2a,FA-11,BioLegend,137011,AF488,AB_2074847,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -P18828,Primary Antibody,CD138,Mouse,Rat,IgG2a,281-2,BioLegend,142526,AF647,AB_2566239,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P18828,Primary Antibody,CD138,Mouse,Rat,IgG2a,281-2,BioLegend,142526,AF647,AB_2566239,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967;0000-0002-1461-0999,NA,0000-0003-4379-8967 Q62230,Primary Antibody,CD169,Mouse,Rat,IgG2a,3D6.112,Bio-Rad,MCA884F,FITC,AB_324246,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 30 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q62230,Primary Antibody,CD169,Mouse,Rat,IgG2a,3D6.112,BioLegend,142416,AF594,AB_2565620,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 for more than 120 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q9JLM8,Primary Antibody,DCAMKL1,Mouse,Rabbit,IgG,Polyclonal,Abcam,ab37994,Unconjugated,AB_873538,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Small Intestine,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 @@ -411,7 +411,7 @@ Q99JB6,Primary Antibody,FOXP3,Mouse,Rat,IgG2a,FJK-16s,Thermo Fisher Scientific,5 Q99JB6,Primary Antibody,FOXP3,Mouse,Rat,IgG2a,FJK-16s,Thermo Fisher Scientific,12-5773-82,PE,AB_465936,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 NA,Primary Antibody,GL-7,Mouse,Rat,IgM,GL7,BD Biosciences,561530,PE,AB_10715834,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P01881,Primary Antibody,IgD,Mouse,Rat,IgG2a,11-26c.2a,BioLegend,405740,AF594,AB_2565572,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 for more than 120 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -E9PVX6,Primary Antibody,Ki-67,Mouse,Mouse,IgG1,B56,BD Biosciences,558616,AF488,AB_647087,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +E9PVX6,Primary Antibody,Ki-67,Mouse,Mouse,IgG1,B56,BD Biosciences,558616,AF488,AB_647087,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967;0000-0002-1461-0999,NA,0000-0003-4379-8967 NA,Primary Antibody,MHC-II,Mouse,Rat,IgG2b,M5/114.15.2,BioLegend,107618,AF647,AB_493525,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q02242,Primary Antibody,PD-1,Mouse,Rat,IgG2a,29F.1.A12,BioLegend,135206,PE,AB_1877231,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q920G3,Primary Antibody,SiglecF,Mouse,Rat,IgG2a,1RNM44N,Thermo Fisher Scientific,56-1702-80,AF700,AB_2637126,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 @@ -1092,3 +1092,11 @@ P08575,Primary Antibody,CD45,Human,Mouse,IgG1,2B11+PD7/26,Novus Biologicals,NBP2 P08575,Primary Antibody,CD45,Human,Mouse,IgG1,2B11+PD7/26,Novus Biologicals,NBP2-34528AF488,AF488,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0009-0006-9784-2694,NA,0009-0006-9784-2694 P16410,Primary Antibody,CTLA4,Human,Rabbit,IgG,CAL49,Abcam,ab270127,AF647,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0009-0006-9784-2694,NA,0009-0006-9784-2694 Q15116,Primary Antibody,PD-1,Human,Rabbit,IgG,EPR4877(2),Abcam,ab275126,AF555,AB_2728811,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0009-0006-9784-2694,NA,0009-0006-9784-2694 +NA,Primary Antibody,B220,Mouse,Rat,IgG2a,RA3-6B2,Biolegend,103226,AF647,AB_389330,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0002-1461-0999,NA,0000-0002-1461-0999 +P06332,Primary Antibody,CD4,Mouse,Rat,IgG2b,GK1.5,BioLegend,100443,BV421,AB_2562557,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0002-1461-0999,NA,0000-0002-1461-0999 +Q08481,Primary Antibody,CD31,Mouse,Rat,IgG2a,MEC13.3,BioLegend,102520,AF594,AB_2563319,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 for more than 120 minutes,Yes,0000-0002-1461-0999,NA,0000-0002-1461-0999 +P06800,Primary Antibody,CD45,Mouse,Rat,IgG2b,30-F11,BioLegend,103144,AF594,AB_2563458,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 for more than 120 minutes,Yes,0000-0002-1461-0999,NA,0000-0002-1461-0999 +P01881,Primary Antibody,IgD,Mouse,Rat,IgG2a,11-26c.2a,BioLegend,405723,BV510,AB_2562742,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0002-1461-0999,NA,0000-0002-1461-0999 +NA,Primary Antibody,CD45.1,Mouse,Mouse,IgG2a,A20,BioLegend,110718,AF488,AB_492862,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0002-8909-5039,NA,0000-0002-8909-5039 +P35762,Primary Antibody,CD81,Mouse,Armenian Hamster,IgG1,Eat-2,BioLegend,104905,PE,AB_2076267,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0002-8909-5039,NA,0000-0002-8909-5039 +P24788,Primary Antibody,CD11b,Mouse,Rat,IgG2b,M1/70,BD Biosciences,101217,PE,AB_396680,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0002-8909-5039,NA,0000-0002-8909-5039 diff --git a/docs/supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0002-1461-0999.md b/docs/supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0002-1461-0999.md new file mode 100644 index 0000000..1344b92 --- /dev/null +++ b/docs/supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0002-1461-0999.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967), [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse small intestine: alpha smooth muscle actin (grey, catalogue number 53-9760-82) | +|:-------:| +| ![](aSMA_AF488_Grey_Mouse_SI.jpg) | diff --git a/docs/supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0003-4379-8967.md b/docs/supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0003-4379-8967.md index 588fe71..1fd5f69 100644 --- a/docs/supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0003-4379-8967.md +++ b/docs/supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0003-4379-8967.md @@ -10,7 +10,7 @@ layout: default | P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications), [1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | | P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | | P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | -| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)], [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | | P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | # Publications diff --git a/docs/supporting_material/Alpha_smooth_muscle_actin_AF488/aSMA_AF488_Grey_Mouse_SI.jpg b/docs/supporting_material/Alpha_smooth_muscle_actin_AF488/aSMA_AF488_Grey_Mouse_SI.jpg new file mode 100644 index 0000000..e26d8c9 Binary files /dev/null and b/docs/supporting_material/Alpha_smooth_muscle_actin_AF488/aSMA_AF488_Grey_Mouse_SI.jpg differ diff --git a/docs/supporting_material/B220_AF647/0000-0002-1461-0999.md b/docs/supporting_material/B220_AF647/0000-0002-1461-0999.md new file mode 100644 index 0000000..d6aa8cb --- /dev/null +++ b/docs/supporting_material/B220_AF647/0000-0002-1461-0999.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Biolegend | 103226 | AF647 | AB_389330 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: B220 (grey, catalog number 103226) | +|:-------:| +| ![](B220_AF647_Grey_Mouse_LN.jpg) | diff --git a/docs/supporting_material/B220_AF647/B220_AF647_Grey_Mouse_LN.jpg b/docs/supporting_material/B220_AF647/B220_AF647_Grey_Mouse_LN.jpg new file mode 100644 index 0000000..eaccca5 Binary files /dev/null and b/docs/supporting_material/B220_AF647/B220_AF647_Grey_Mouse_LN.jpg differ diff --git a/docs/supporting_material/CD11b_PE/0000-0002-8909-5039.md b/docs/supporting_material/CD11b_PE/0000-0002-8909-5039.md new file mode 100644 index 0000000..040fb59 --- /dev/null +++ b/docs/supporting_material/CD11b_PE/0000-0002-8909-5039.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BD Biosciences | 101217 | PE | AB_396680 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-8909-5039](https://orcid.org/0000-0002-8909-5039) | NA | [0000-0002-8909-5039](https://orcid.org/0000-0002-8909-5039) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: CD11b (grey, catalog number 101217) | +|:-------:| +| ![](CD11b_PE_Grey_Mouse_LN.jpg) | diff --git a/docs/supporting_material/CD11b_PE/CD11b_PE_Grey_Mouse_LN.jpg b/docs/supporting_material/CD11b_PE/CD11b_PE_Grey_Mouse_LN.jpg new file mode 100644 index 0000000..e5729ed Binary files /dev/null and b/docs/supporting_material/CD11b_PE/CD11b_PE_Grey_Mouse_LN.jpg differ diff --git a/docs/supporting_material/CD11c_AF488/0000-0002-1461-0999.md b/docs/supporting_material/CD11c_AF488/0000-0002-1461-0999.md new file mode 100644 index 0000000..ad3d80f --- /dev/null +++ b/docs/supporting_material/CD11c_AF488/0000-0002-1461-0999.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:-----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:------------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | Thermo Fisher Scientific | MCD11c20 | AF488 | AB_10373244 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: CD11c (grey, catalog number MCD11c20) | +|:-------:| +| ![](CD11c_AF488_Grey_Mouse_LN.jpg) | diff --git a/docs/supporting_material/CD11c_AF488/0000-0003-4379-8967.md b/docs/supporting_material/CD11c_AF488/0000-0003-4379-8967.md index 0c36724..8e7bb0d 100644 --- a/docs/supporting_material/CD11c_AF488/0000-0003-4379-8967.md +++ b/docs/supporting_material/CD11c_AF488/0000-0003-4379-8967.md @@ -6,7 +6,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:-----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:------------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:------------| -| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | Thermo Fisher Scientific | MCD11c20 | AF488 | AB_10373244 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | +| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | Thermo Fisher Scientific | MCD11c20 | AF488 | AB_10373244 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)]; [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | # Publications diff --git a/docs/supporting_material/CD11c_AF488/CD11c_AF488_Grey_Mouse_LN.jpg b/docs/supporting_material/CD11c_AF488/CD11c_AF488_Grey_Mouse_LN.jpg new file mode 100644 index 0000000..4330f22 Binary files /dev/null and b/docs/supporting_material/CD11c_AF488/CD11c_AF488_Grey_Mouse_LN.jpg differ diff --git a/docs/supporting_material/CD200_BV421/0000-0002-1461-0999.md b/docs/supporting_material/CD200_BV421/0000-0002-1461-0999.md new file mode 100644 index 0000000..fc16407 --- /dev/null +++ b/docs/supporting_material/CD200_BV421/0000-0002-1461-0999.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| Q9ES57 | Primary Antibody | CD200 | Mouse | Rat | IgG2a | OX-90 | BD Biosciences | 565547 | BV421 | AB_2739289 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: CD11c (grey, catalog number 565547) | +|:-------:| +| ![](CD200_BV421_Grey_Mouse_LN.jpg) | diff --git a/docs/supporting_material/CD200_BV421/0000-0003-4379-8967.md b/docs/supporting_material/CD200_BV421/0000-0003-4379-8967.md index 4ecadca..8ad4d0e 100644 --- a/docs/supporting_material/CD200_BV421/0000-0003-4379-8967.md +++ b/docs/supporting_material/CD200_BV421/0000-0003-4379-8967.md @@ -6,7 +6,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:------------| -| Q9ES57 | Primary Antibody | CD200 | Mouse | Rat | IgG2a | OX-90 | BD Biosciences | 565547 | BV421 | AB_2739289 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | +| Q9ES57 | Primary Antibody | CD200 | Mouse | Rat | IgG2a | OX-90 | BD Biosciences | 565547 | BV421 | AB_2739289 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)]; [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | # Publications diff --git a/docs/supporting_material/CD200_BV421/CD200_BV421_Grey_Mouse_LN.jpg b/docs/supporting_material/CD200_BV421/CD200_BV421_Grey_Mouse_LN.jpg new file mode 100644 index 0000000..1a868c7 Binary files /dev/null and b/docs/supporting_material/CD200_BV421/CD200_BV421_Grey_Mouse_LN.jpg differ diff --git a/docs/supporting_material/CD23_AF647/0000-0002-8909-5039.md b/docs/supporting_material/CD23_AF647/0000-0002-8909-5039.md new file mode 100644 index 0000000..0aa6874 --- /dev/null +++ b/docs/supporting_material/CD23_AF647/0000-0002-8909-5039.md @@ -0,0 +1,26 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| P20693 | Primary Antibody | CD23 | Mouse | Rat | IgG2a | B3B4 | BioLegend | 101611 | AF647 | AB_493479 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0002-8909-5039](https://orcid.org/0000-0002-8909-5039) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse (naive) lymph node: CD23 (grey, catalog number 101611) | +|:-------:| +| ![](CD23_AF647_Grey_Mouse_LN_2_Large.jpg) | + +| Mouse (immunized) lymph node: CD23 (grey, catalog number 101611) | +|:-------:| +| ![](CD23_AF647_Grey_Mouse_LN_1_Large.jpg) | diff --git a/docs/supporting_material/CD23_AF647/0000-0003-4379-8967.md b/docs/supporting_material/CD23_AF647/0000-0003-4379-8967.md index 144d231..97b9d99 100644 --- a/docs/supporting_material/CD23_AF647/0000-0003-4379-8967.md +++ b/docs/supporting_material/CD23_AF647/0000-0003-4379-8967.md @@ -6,7 +6,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:------------| -| P20693 | Primary Antibody | CD23 | Mouse | Rat | IgG2a | B3B4 | BioLegend | 101611 | AF647 | AB_493479 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | +| P20693 | Primary Antibody | CD23 | Mouse | Rat | IgG2a | B3B4 | BioLegend | 101611 | AF647 | AB_493479 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)]; [0000-0002-8909-5039](https://orcid.org/0000-0002-8909-5039) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | # Publications diff --git a/docs/supporting_material/CD23_AF647/CD23_AF647_Grey_Mouse_LN_1_Large.jpg b/docs/supporting_material/CD23_AF647/CD23_AF647_Grey_Mouse_LN_1_Large.jpg new file mode 100644 index 0000000..6fe0942 Binary files /dev/null and b/docs/supporting_material/CD23_AF647/CD23_AF647_Grey_Mouse_LN_1_Large.jpg differ diff --git a/docs/supporting_material/CD23_AF647/CD23_AF647_Grey_Mouse_LN_2_Large.jpg b/docs/supporting_material/CD23_AF647/CD23_AF647_Grey_Mouse_LN_2_Large.jpg new file mode 100644 index 0000000..8e65ded Binary files /dev/null and b/docs/supporting_material/CD23_AF647/CD23_AF647_Grey_Mouse_LN_2_Large.jpg differ diff --git a/docs/supporting_material/CD31_AF594/0000-0002-1461-0999.md b/docs/supporting_material/CD31_AF594/0000-0002-1461-0999.md new file mode 100644 index 0000000..9a685dd --- /dev/null +++ b/docs/supporting_material/CD31_AF594/0000-0002-1461-0999.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:----------------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102520 | AF594 | AB_2563319 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: CD31 (grey, catalog number 102520) | +|:-------:| +| ![](CD31_AF594_Grey_Mouse_LN.jpg) | diff --git a/docs/supporting_material/CD31_AF594/CD31_AF594_Grey_Mouse_LN.jpg b/docs/supporting_material/CD31_AF594/CD31_AF594_Grey_Mouse_LN.jpg new file mode 100644 index 0000000..e8751df Binary files /dev/null and b/docs/supporting_material/CD31_AF594/CD31_AF594_Grey_Mouse_LN.jpg differ diff --git a/docs/supporting_material/CD35_BV510/0000-0002-1461-0999.md b/docs/supporting_material/CD35_BV510/0000-0002-1461-0999.md new file mode 100644 index 0000000..21d9787 --- /dev/null +++ b/docs/supporting_material/CD35_BV510/0000-0002-1461-0999.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | BD Biosciences | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432); [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999)| NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse (immunized) lymph node: CD35 (green, catalog number 740132) and DAPI (blue, catalog number 62248) | +|:-------:| +| ![](CD35_BV510_Green_DAPI_Blue_Mouse_LN.jpg) | diff --git a/docs/supporting_material/CD35_BV510/0000-0003-1118-7432.md b/docs/supporting_material/CD35_BV510/0000-0003-1118-7432.md index 81dbefc..2535829 100644 --- a/docs/supporting_material/CD35_BV510/0000-0003-1118-7432.md +++ b/docs/supporting_material/CD35_BV510/0000-0003-1118-7432.md @@ -6,7 +6,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| -| NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | BD Biosciences | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | BD Biosciences | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432); [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | # Publications diff --git a/docs/supporting_material/CD35_BV510/0000-0003-4379-8967.md b/docs/supporting_material/CD35_BV510/0000-0003-4379-8967.md index e6fffc4..8a8ddb1 100644 --- a/docs/supporting_material/CD35_BV510/0000-0003-4379-8967.md +++ b/docs/supporting_material/CD35_BV510/0000-0003-4379-8967.md @@ -7,7 +7,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:------------| | NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | BD Biosciences | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | -| NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | BD Biosciences | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)]; [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | BD Biosciences | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)]; [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432); [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | # Publications diff --git a/docs/supporting_material/CD35_BV510/CD35_BV510_Green_DAPI_Blue_Mouse_LN.jpg b/docs/supporting_material/CD35_BV510/CD35_BV510_Green_DAPI_Blue_Mouse_LN.jpg new file mode 100644 index 0000000..4e33f0a Binary files /dev/null and b/docs/supporting_material/CD35_BV510/CD35_BV510_Green_DAPI_Blue_Mouse_LN.jpg differ diff --git a/docs/supporting_material/CD3_AF488/0000-0002-1461-0999.md b/docs/supporting_material/CD3_AF488/0000-0002-1461-0999.md new file mode 100644 index 0000000..b5e804d --- /dev/null +++ b/docs/supporting_material/CD3_AF488/0000-0002-1461-0999.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100210 | AF488 | AB_389301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: CD3 (grey, catalog number 100210) | +|:-------:| +| ![](CD3_488_Grey_Mouse_LN.jpg) | diff --git a/docs/supporting_material/CD3_AF488/0000-0003-4379-8967.md b/docs/supporting_material/CD3_AF488/0000-0003-4379-8967.md index 82243ec..10686c0 100644 --- a/docs/supporting_material/CD3_AF488/0000-0003-4379-8967.md +++ b/docs/supporting_material/CD3_AF488/0000-0003-4379-8967.md @@ -6,7 +6,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:--------------------|:------------| -| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100210 | AF488 | AB_389301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100210 | AF488 | AB_389301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications)]; [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | | P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300415 | AF488 | AB_389310 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications), [1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | # Publications diff --git a/docs/supporting_material/CD3_AF488/CD3_488_Grey_Mouse_LN.jpg b/docs/supporting_material/CD3_AF488/CD3_488_Grey_Mouse_LN.jpg new file mode 100644 index 0000000..d844a67 Binary files /dev/null and b/docs/supporting_material/CD3_AF488/CD3_488_Grey_Mouse_LN.jpg differ diff --git a/docs/supporting_material/CD45.1_AF488/0000-0002-8909-5039.md b/docs/supporting_material/CD45.1_AF488/0000-0002-8909-5039.md new file mode 100644 index 0000000..0781354 --- /dev/null +++ b/docs/supporting_material/CD45.1_AF488/0000-0002-8909-5039.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| NA | Primary Antibody | CD45.1 | Mouse | Mouse | IgG2a | A20 | BioLegend | 110718 | AF488 | AB_492862 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-8909-5039](https://orcid.org/0000-0002-8909-5039) | NA | [0000-0002-8909-5039](https://orcid.org/0000-0002-8909-5039) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse (immunized) lymph node: CD45.1 (yellow, catalog number 110718) and CD138 (magenta, catalog number 142526) | +|:-------:| +| ![](CD45.1_AF488_Magenta_CD138_AF647_Yellow_Mouse_LN.jpg) | diff --git a/docs/supporting_material/CD45.1_AF488/CD45.1_AF488_Magenta_CD138_AF647_Yellow_Mouse_LN.jpg b/docs/supporting_material/CD45.1_AF488/CD45.1_AF488_Magenta_CD138_AF647_Yellow_Mouse_LN.jpg new file mode 100644 index 0000000..57f16d3 Binary files /dev/null and b/docs/supporting_material/CD45.1_AF488/CD45.1_AF488_Magenta_CD138_AF647_Yellow_Mouse_LN.jpg differ diff --git a/docs/supporting_material/CD45_AF594/0000-0002-1461-0999.md b/docs/supporting_material/CD45_AF594/0000-0002-1461-0999.md new file mode 100644 index 0000000..e7eee9f --- /dev/null +++ b/docs/supporting_material/CD45_AF594/0000-0002-1461-0999.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:----------------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103144 | AF594 | AB_2563458 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: CD45 (grey, catalog number 103144) | +|:-------:| +| ![](CD45_AF594_Grey_Mouse_LN.jpg) | diff --git a/docs/supporting_material/CD45_AF594/CD45_AF594_Grey_Mouse_LN.jpg b/docs/supporting_material/CD45_AF594/CD45_AF594_Grey_Mouse_LN.jpg new file mode 100644 index 0000000..f1ba8af Binary files /dev/null and b/docs/supporting_material/CD45_AF594/CD45_AF594_Grey_Mouse_LN.jpg differ diff --git a/docs/supporting_material/CD4_BV421/0000-0002-1461-0999.md b/docs/supporting_material/CD4_BV421/0000-0002-1461-0999.md new file mode 100644 index 0000000..9ae8f03 --- /dev/null +++ b/docs/supporting_material/CD4_BV421/0000-0002-1461-0999.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BioLegend | 100443 | BV421 | AB_2562557 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: CD4 (grey, catalog number 100449) | +|:-------:| +| ![](CD4_BV421_Grey_Mouse_LN_large.jpg) | diff --git a/docs/supporting_material/CD4_BV421/CD4_BV421_Grey_Mouse_LN_large.jpg b/docs/supporting_material/CD4_BV421/CD4_BV421_Grey_Mouse_LN_large.jpg new file mode 100644 index 0000000..97347c7 Binary files /dev/null and b/docs/supporting_material/CD4_BV421/CD4_BV421_Grey_Mouse_LN_large.jpg differ diff --git a/docs/supporting_material/CD4_BV510/0000-0002-1461-0999.md b/docs/supporting_material/CD4_BV510/0000-0002-1461-0999.md new file mode 100644 index 0000000..57e73b9 --- /dev/null +++ b/docs/supporting_material/CD4_BV510/0000-0002-1461-0999.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BioLegend | 100449 | BV510 | AB_2564587 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: CD4 (grey, catalog number 100443) | +|:-------:| +| ![](CD4_BV510_Grey_Mouse_LN.jpg) | diff --git a/docs/supporting_material/CD4_BV510/0000-0003-4379-8967.md b/docs/supporting_material/CD4_BV510/0000-0003-4379-8967.md index 40c3448..b3952f7 100644 --- a/docs/supporting_material/CD4_BV510/0000-0003-4379-8967.md +++ b/docs/supporting_material/CD4_BV510/0000-0003-4379-8967.md @@ -6,7 +6,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:------------| -| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BioLegend | 100449 | BV510 | AB_2564587 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | +| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BioLegend | 100449 | BV510 | AB_2564587 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | # Publications diff --git a/docs/supporting_material/CD4_BV510/CD4_BV510_Grey_Mouse_LN.jpg b/docs/supporting_material/CD4_BV510/CD4_BV510_Grey_Mouse_LN.jpg new file mode 100644 index 0000000..b5f1f64 Binary files /dev/null and b/docs/supporting_material/CD4_BV510/CD4_BV510_Grey_Mouse_LN.jpg differ diff --git a/docs/supporting_material/CD64_AF647/0000-0002-1461-0999.md b/docs/supporting_material/CD64_AF647/0000-0002-1461-0999.md new file mode 100644 index 0000000..50074ba --- /dev/null +++ b/docs/supporting_material/CD64_AF647/0000-0002-1461-0999.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| P26151 | Primary Antibody | CD64 | Mouse | Mouse | IgG1 | X54-5/7.1 | BioLegend | 139322 | AF647 | AB_2566561 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: CD64 (grey, catalog number 139322) | +|:-------:| +| ![](CD64_AF647_Grey_Mouse_LN.jpg) | diff --git a/docs/supporting_material/CD64_AF647/0000-0003-4379-8967.md b/docs/supporting_material/CD64_AF647/0000-0003-4379-8967.md index ecff398..1d424ab 100644 --- a/docs/supporting_material/CD64_AF647/0000-0003-4379-8967.md +++ b/docs/supporting_material/CD64_AF647/0000-0003-4379-8967.md @@ -6,7 +6,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:------------| -| P26151 | Primary Antibody | CD64 | Mouse | Mouse | IgG1 | X54-5/7.1 | BioLegend | 139322 | AF647 | AB_2566561 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | +| P26151 | Primary Antibody | CD64 | Mouse | Mouse | IgG1 | X54-5/7.1 | BioLegend | 139322 | AF647 | AB_2566561 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)]; [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | # Publications diff --git a/docs/supporting_material/CD64_AF647/CD64_AF647_Grey_Mouse_LN.jpg b/docs/supporting_material/CD64_AF647/CD64_AF647_Grey_Mouse_LN.jpg new file mode 100644 index 0000000..02b5a9f Binary files /dev/null and b/docs/supporting_material/CD64_AF647/CD64_AF647_Grey_Mouse_LN.jpg differ diff --git a/docs/supporting_material/CD81_PE/0000-0002-8909-5039.md b/docs/supporting_material/CD81_PE/0000-0002-8909-5039.md new file mode 100644 index 0000000..033b8a2 --- /dev/null +++ b/docs/supporting_material/CD81_PE/0000-0002-8909-5039.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:-----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| P35762 | Primary Antibody | CD81 | Mouse/Rat | Armenian Hamster | IgG1 | Eat-2 | BioLegend | 104905 | PE | AB_2076267 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-8909-5039](https://orcid.org/0000-0002-8909-5039) | NA | [0000-0002-8909-5039](https://orcid.org/0000-0002-8909-5039) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: CD3 (grey, catalog number 104905) | +|:-------:| +| ![](CD81_PE_Grey_Mouse_LN.jpg) | diff --git a/docs/supporting_material/CD81_PE/CD81_PE_Grey_Mouse_LN.jpg b/docs/supporting_material/CD81_PE/CD81_PE_Grey_Mouse_LN.jpg new file mode 100644 index 0000000..79050b0 Binary files /dev/null and b/docs/supporting_material/CD81_PE/CD81_PE_Grey_Mouse_LN.jpg differ diff --git a/docs/supporting_material/IgD_BV510/0000-0002-1461-0999.md b/docs/supporting_material/IgD_BV510/0000-0002-1461-0999.md new file mode 100644 index 0000000..9a847b3 --- /dev/null +++ b/docs/supporting_material/IgD_BV510/0000-0002-1461-0999.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405723 | BV510 | AB_2562742 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: IgD (green, catalog number 405723) and B220 (blue, catalog number 103226) | +|:-------:| +| ![](IgD_BV510_Green_B220_AF647_Blue_Mouse_LN.jpg) | diff --git a/docs/supporting_material/IgD_BV510/IgD_BV510_Green_B220_AF647_Blue_Mouse_LN.jpg b/docs/supporting_material/IgD_BV510/IgD_BV510_Green_B220_AF647_Blue_Mouse_LN.jpg new file mode 100644 index 0000000..176fb2e Binary files /dev/null and b/docs/supporting_material/IgD_BV510/IgD_BV510_Green_B220_AF647_Blue_Mouse_LN.jpg differ diff --git a/docs/supporting_material/Ki-67_AF488/0000-0002-1461-0999.md b/docs/supporting_material/Ki-67_AF488/0000-0002-1461-0999.md new file mode 100644 index 0000000..1c67a6c --- /dev/null +++ b/docs/supporting_material/Ki-67_AF488/0000-0002-1461-0999.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| E9PVX6 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 558616 | AF488 | AB_647087 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: Ki67 (grey, catalog number 558616) | +|:-------:| +| ![](Ki67_AF488_Grey_Mouse_LN.jpg) | diff --git a/docs/supporting_material/Ki-67_AF488/0000-0003-4379-8967.md b/docs/supporting_material/Ki-67_AF488/0000-0003-4379-8967.md index 70b4536..6363471 100644 --- a/docs/supporting_material/Ki-67_AF488/0000-0003-4379-8967.md +++ b/docs/supporting_material/Ki-67_AF488/0000-0003-4379-8967.md @@ -6,7 +6,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:--------| -| E9PVX6 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 558616 | AF488 | AB_647087 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| E9PVX6 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 558616 | AF488 | AB_647087 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)]; [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | # Publications diff --git a/docs/supporting_material/Ki-67_AF488/Ki67_AF488_Grey_Mouse_LN.jpg b/docs/supporting_material/Ki-67_AF488/Ki67_AF488_Grey_Mouse_LN.jpg new file mode 100644 index 0000000..9528c87 Binary files /dev/null and b/docs/supporting_material/Ki-67_AF488/Ki67_AF488_Grey_Mouse_LN.jpg differ diff --git a/docs/supporting_material/Pax5_AF647/0000-0002-1461-0999.md b/docs/supporting_material/Pax5_AF647/0000-0002-1461-0999.md new file mode 100644 index 0000000..3156c92 --- /dev/null +++ b/docs/supporting_material/Pax5_AF647/0000-0002-1461-0999.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| Q02650 | Primary Antibody | Pax5 | Mouse | Rat | IgG2a | 1H9 | BioLegend | 649704 | AF647 | AB_2562425 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: Pax5 (magenta, catalog number 649704) and CD3 (cyan, catalog number 100210) | +|:-------:| +| ![](Pax5_AF647_Margenta_CD3_AF488_Cyan_Mouse_LN.jpg) | diff --git a/docs/supporting_material/Pax5_AF647/0000-0003-4379-8967.md b/docs/supporting_material/Pax5_AF647/0000-0003-4379-8967.md index 6949ca6..471d501 100644 --- a/docs/supporting_material/Pax5_AF647/0000-0003-4379-8967.md +++ b/docs/supporting_material/Pax5_AF647/0000-0003-4379-8967.md @@ -6,7 +6,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:--------| -| Q02650 | Primary Antibody | Pax5 | Mouse | Rat | IgG2a | 1H9 | BioLegend | 649704 | AF647 | AB_2562425 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| Q02650 | Primary Antibody | Pax5 | Mouse | Rat | IgG2a | 1H9 | BioLegend | 649704 | AF647 | AB_2562425 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)]; [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | # Publications diff --git a/docs/supporting_material/Pax5_AF647/Pax5_AF647_Margenta_CD3_AF488_Cyan_Mouse_LN.jpg b/docs/supporting_material/Pax5_AF647/Pax5_AF647_Margenta_CD3_AF488_Cyan_Mouse_LN.jpg new file mode 100644 index 0000000..db71cb6 Binary files /dev/null and b/docs/supporting_material/Pax5_AF647/Pax5_AF647_Margenta_CD3_AF488_Cyan_Mouse_LN.jpg differ