From fcb3d98976d3a0b6e6811c42f7c7c1029f08c9e4 Mon Sep 17 00:00:00 2001 From: Ziv Yaniv Date: Wed, 14 Feb 2024 13:31:37 -0500 Subject: [PATCH] Adding CD49a PE lymph node configuration. --- data/reagent_resources.csv | 1 + .../CD49a_PE/0000-0003-4379-8967.md | 30 ++++++++++++++++++ ...egend_303133_CD49a_PE_BioLegend_328303.jpg | Bin 0 -> 66631 bytes 3 files changed, 31 insertions(+) create mode 100644 docs/supporting_material/CD49a_PE/0000-0003-4379-8967.md create mode 100755 docs/supporting_material/CD49a_PE/CD31_AF700_BioLegend_303133_CD49a_PE_BioLegend_328303.jpg diff --git a/data/reagent_resources.csv b/data/reagent_resources.csv index c1c8638..03cfec3 100644 --- a/data/reagent_resources.csv +++ b/data/reagent_resources.csv @@ -1073,3 +1073,4 @@ P70658,Primary Antibody,CXCR4,Mouse,Rabbit,IgG,polyclonal,Novus Biologicals,NB-1 P56485,Primary Antibody,CXCR7,Mouse,Rabbit,IgG,polyclonal,Novus Biologicals,NBP2-24779G,DyLight 488,NA,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997 Q9QXH4,Primary Antibody,CD11c,Mouse,Armenian Hamster,IgG,N418,BioLegend,117314,AF647,AB_492850,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997 P03995,Primary Antibody,GFAP,Mouse,Mouse,IgG1,GA5,Thermo Fisher Scientific,41-9892-82,eF570,AB_2573656,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997 +P56199,Primary Antibody,CD49a,Human,Mouse,IgG1,TS2/7,BioLegend,328303,PE,AB_1236441,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 diff --git a/docs/supporting_material/CD49a_PE/0000-0003-4379-8967.md b/docs/supporting_material/CD49a_PE/0000-0003-4379-8967.md new file mode 100644 index 0000000..bae6327 --- /dev/null +++ b/docs/supporting_material/CD49a_PE/0000-0003-4379-8967.md @@ -0,0 +1,30 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:--------------------------------------------------------------------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328303 | PE | AB_1236441 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications), [2](#publications), [1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + +1. A. J. Radtke et al., "A Multi-scale, Multiomic Atlas of Human Normal and Follicular Lymphoma Lymph Nodes", *bioRxiv*, 2022, [doi: 10.1101/2022.06.03.494716](https://doi.org/10.1101/2022.06.03.494716). +2. A. J. Radtke et al., "IBEX: an iterative immunolabeling and chemical bleaching + method for high-content imaging of diverse tissues", *Nat. Protoc.*, 17(2):378-401, 2022, [doi: 10.1038/s41596-021-00644-9](https://doi.org/10.1038/s41596-021-00644-9). + + A. J. Radtke et al., "Accompanying dataset for: IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues", [doi: 10.5281/zenodo.5244550](https://doi.org/10.5281/zenodo.5244551). + +3. A. J. Radtke et al., "IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues", *Proc Natl Acad Sci*, 117(52):33455–33465, 2020, [doi:10.1073/pnas.2018488117](https://doi.org/10.1073/pnas.2018488117). + + +# Additional Notes + + + + +| Human lymph node: CD49a (cyan, catalog number 328303) and CD31 (red, catalog number 303133) | +|:-------:| +| ![](CD31_AF700_BioLegend_303133_CD49a_PE_BioLegend_328303.jpg) | diff --git a/docs/supporting_material/CD49a_PE/CD31_AF700_BioLegend_303133_CD49a_PE_BioLegend_328303.jpg b/docs/supporting_material/CD49a_PE/CD31_AF700_BioLegend_303133_CD49a_PE_BioLegend_328303.jpg new file mode 100755 index 0000000000000000000000000000000000000000..ef734997f677bc59984169e2fbef189ac6801460 GIT binary patch literal 66631 zcmcG$2Ut^C*ESq_M=7BRiu5Fcbg2%-011SW5ITxXiTBsG~E^^FHtUU!Pw4I>{k9XRm$My4StR&L2~MEP(bR%@AfF 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