From 76856b8bdcb4ec19422335f0571f04c2aeeaf6d2 Mon Sep 17 00:00:00 2001 From: Ziv Yaniv Date: Tue, 27 Feb 2024 11:46:34 -0500 Subject: [PATCH] Adding reagent configurations. Added configurations for lymph node with Cell DIVE-IBEX method. --- data/reagent_resources.csv | 7 +++++++ .../BCL2_Unconjugated/0000-0003-4379-8967.md | 12 ++++++++++++ ...CD3_ab208514_CD4_ab196372_CD21_ab306325.jpg | Bin 0 -> 181545 bytes ...CD3_ab208514_CD4_ab196372_CD21_ab306325.jpg | Bin 0 -> 162395 bytes .../CD21_PE/0000-0003-4379-8967.md | 7 +++++++ .../CD3_AF555/0000-0003-4379-8967.md | 13 +++++++++++++ .../CD4_AF488/0000-0003-4379-8967.md | 13 +++++++++++++ 7 files changed, 52 insertions(+) create mode 100644 docs/supporting_material/BCL2_Unconjugated/Human_FL_LN_BCL2_ab236221_CD3_ab208514_CD4_ab196372_CD21_ab306325.jpg create mode 100644 docs/supporting_material/BCL2_Unconjugated/Human_normal_LN_BCL2_ab236221_CD3_ab208514_CD4_ab196372_CD21_ab306325.jpg diff --git a/data/reagent_resources.csv b/data/reagent_resources.csv index 42ee36a..db5c457 100644 --- a/data/reagent_resources.csv +++ b/data/reagent_resources.csv @@ -1074,3 +1074,10 @@ P56485,Primary Antibody,CXCR7,Mouse,Rabbit,IgG,polyclonal,Novus Biologicals,NBP2 Q9QXH4,Primary Antibody,CD11c,Mouse,Armenian Hamster,IgG,N418,BioLegend,117314,AF647,AB_492850,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997 P03995,Primary Antibody,GFAP,Mouse,Mouse,IgG1,GA5,Thermo Fisher Scientific,41-9892-82,eF570,AB_2573656,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997 P56199,Primary Antibody,CD49a,Human,Mouse,IgG1,TS2/7,BioLegend,328303,PE,AB_1236441,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P10415,Primary Antibody,BCL2,Human,Rabbit,IgG,SP66,Abcam,ab236221,Unconjugated,NA,Stock,Cell DIVE-IBEX,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P10415,Primary Antibody,BCL2,Human,Rabbit,IgG,SP66,Abcam,ab236221,Unconjugated,NA,Stock,Cell DIVE-IBEX,FFPE,Lymph Node,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P07766,Primary Antibody,CD3,Human,Rabbit,IgG,EP4426,Abcam,ab208514,AF555,AB_443425,Stock,Cell DIVE-IBEX,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P07766,Primary Antibody,CD3,Human,Rabbit,IgG,EP4426,Abcam,ab208514,AF555,AB_443425,Stock,Cell DIVE-IBEX,FFPE,Lymph Node,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P01730,Primary Antibody,CD4,Human,Rabbit,IgG,EPR6855,Abcam,ab196372,AF488,AB_2889191,Stock,Cell DIVE-IBEX,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P01730,Primary Antibody,CD4,Human,Rabbit,IgG,EPR6855,Abcam,ab196372,AF488,AB_2889191,Stock,Cell DIVE-IBEX,FFPE,Lymph Node,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P20023,Primary Antibody,CD21,Human,Rabbit,IgG,SP186,Abcam,ab306325,PE,NA,Stock,Cell DIVE-IBEX,FFPE,Lymph Node,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 diff --git a/docs/supporting_material/BCL2_Unconjugated/0000-0003-4379-8967.md b/docs/supporting_material/BCL2_Unconjugated/0000-0003-4379-8967.md index e823012..c82459a 100644 --- a/docs/supporting_material/BCL2_Unconjugated/0000-0003-4379-8967.md +++ b/docs/supporting_material/BCL2_Unconjugated/0000-0003-4379-8967.md @@ -8,6 +8,8 @@ layout: default |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------|:-----------------|:-------------|:-------|:---------------|:-----------------------|:----------------------|:----------------|:--------------------|:------------------|:-------------------------------------------------------------------------------------------|:------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:------------| | P10415 | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | Abcam | ab236221 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | | P10415 | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | Abcam | ab236221 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| P10415 | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | Abcam | ab236221 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| P10415 | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | Abcam | ab236221 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | # Publications @@ -19,3 +21,13 @@ layout: default 1. Validated in human follicular lymphoma FFPE samples; amplified with donkey anti-rabbit AF594 (Thermo Fisher Scientific A-21207). + + +| Human normal LN: BCL2 (cyan, catalog number ab236221 and A-31573), CD3 (magenta, catalog number ab208514), CD4 (blue, catalog number ab196372), CD21 (yellow, catalog number ab306325) | +|:-------:| +| ![](Human_normal_LN_BCL2_ab236221_CD3_ab208514_CD4_ab196372_CD21_ab306325.jpg) | + + +| Human FL LN: BCL2 (cyan, catalog number ab236221 and A-31573), CD3 (magenta, catalog number ab208514), CD4 (blue, catalog number ab196372), CD21 (yellow, catalog number ab306325) | +|:-------:| +| ![](Human_FL_LN_BCL2_ab236221_CD3_ab208514_CD4_ab196372_CD21_ab306325.jpg) | diff --git a/docs/supporting_material/BCL2_Unconjugated/Human_FL_LN_BCL2_ab236221_CD3_ab208514_CD4_ab196372_CD21_ab306325.jpg b/docs/supporting_material/BCL2_Unconjugated/Human_FL_LN_BCL2_ab236221_CD3_ab208514_CD4_ab196372_CD21_ab306325.jpg new file mode 100644 index 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100644 --- a/docs/supporting_material/CD21_PE/0000-0003-4379-8967.md +++ b/docs/supporting_material/CD21_PE/0000-0003-4379-8967.md @@ -9,6 +9,7 @@ layout: default | P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354903 | PE | AB_2561406 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications), [1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | | P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) | | P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | No | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) | +| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | # Publications @@ -20,6 +21,7 @@ layout: default 2. A. J. Radtke et al., "IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues", *Proc Natl Acad Sci*, 117(52):33455–33465, 2020, [doi:10.1073/pnas.2018488117](https://doi.org/10.1073/pnas.2018488117) +3. A. J. Radtke et al., "A Multi-scale, Multiomic Atlas of Human Normal and Follicular Lymphoma Lymph Nodes", *bioRxiv*, 2022, [doi: 10.1101/2022.06.03.494716](https://doi.org/10.1101/2022.06.03.494716). # Additional Notes @@ -31,3 +33,8 @@ layout: default |:-------:|:-------:| | ![CD21 PE FL pH6and9](CD21_PE_FL_pH6and9.jpg) | ![CD21 PE FL pH6](CD21_PE_FL_pH6.jpg) | + + +| Human normal LN: BCL2 (cyan, catalog number ab236221 and A-31573), CD3 (magenta, catalog number ab208514), CD4 (blue, catalog number ab196372), CD21 (yellow, catalog number ab306325) | +|:-------:| +| ![](../BCL2_Unconjugated/Human_normal_LN_BCL2_ab236221_CD3_ab208514_CD4_ab196372_CD21_ab306325.jpg) | diff --git a/docs/supporting_material/CD3_AF555/0000-0003-4379-8967.md b/docs/supporting_material/CD3_AF555/0000-0003-4379-8967.md index f4daa48..4d49b78 100644 --- a/docs/supporting_material/CD3_AF555/0000-0003-4379-8967.md +++ b/docs/supporting_material/CD3_AF555/0000-0003-4379-8967.md @@ -12,6 +12,8 @@ layout: default | P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) | | P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [4](#notes) | | P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [5](#notes) | +| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | # Publications @@ -23,6 +25,7 @@ layout: default 2. A. J. Radtke et al., "IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues", *Proc Natl Acad Sci*, 117(52):33455–33465, 2020, [doi:10.1073/pnas.2018488117](https://doi.org/10.1073/pnas.2018488117) +3. A. J. Radtke et al., "A Multi-scale, Multiomic Atlas of Human Normal and Follicular Lymphoma Lymph Nodes", *bioRxiv*, 2022, [doi: 10.1101/2022.06.03.494716](https://doi.org/10.1101/2022.06.03.494716). # Additional Notes @@ -32,3 +35,13 @@ layout: default 3. Custom conjugate by BioLegend. Labels T cells in diverse tissues. 4. Antibody labels T cells. Preferred clone for FFPE samples. Works beautifully on Cell DIVE imager with IBEX dye inactivation protocol. 5. Labels T cells in lung. Great signal and works very well on Cell DIVE imager with IBEX dye inactivation protocol. + + +| Human normal LN: BCL2 (cyan, catalog number ab236221 and A-31573), CD3 (magenta, catalog number ab208514), CD4 (blue, catalog number ab196372), CD21 (yellow, catalog number ab306325) | +|:-------:| +| ![](../BCL2_Unconjugated/Human_normal_LN_BCL2_ab236221_CD3_ab208514_CD4_ab196372_CD21_ab306325.jpg) | + + +| Human FL LN: BCL2 (cyan, catalog number ab236221 and A-31573), CD3 (magenta, catalog number ab208514), CD4 (blue, catalog number ab196372), CD21 (yellow, catalog number ab306325) | +|:-------:| +| ![](../BCL2_Unconjugated/Human_FL_LN_BCL2_ab236221_CD3_ab208514_CD4_ab196372_CD21_ab306325.jpg) | diff --git a/docs/supporting_material/CD4_AF488/0000-0003-4379-8967.md b/docs/supporting_material/CD4_AF488/0000-0003-4379-8967.md index acbf2c0..7f7e487 100644 --- a/docs/supporting_material/CD4_AF488/0000-0003-4379-8967.md +++ b/docs/supporting_material/CD4_AF488/0000-0003-4379-8967.md @@ -10,6 +10,8 @@ layout: default | P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BD Biosciences | 557667 | AF488 | AB_396779 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | | P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Abcam | ab196372 | AF488 | AB_2889191 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | | P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Abcam | ab196372 | AF488 | AB_2889191 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) | +| P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Abcam | ab196372 | AF488 | AB_2889191 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Abcam | ab196372 | AF488 | AB_2889191 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | # Publications @@ -21,6 +23,7 @@ layout: default 2. A. J. Radtke et al., "IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues", *Proc Natl Acad Sci*, 117(52):33455–33465, 2020, [doi:10.1073/pnas.2018488117](https://doi.org/10.1073/pnas.2018488117) +3. A. J. Radtke et al., "A Multi-scale, Multiomic Atlas of Human Normal and Follicular Lymphoma Lymph Nodes", *bioRxiv*, 2022, [doi: 10.1101/2022.06.03.494716](https://doi.org/10.1101/2022.06.03.494716). # Additional Notes @@ -31,3 +34,13 @@ layout: default | Human lymph node: CD163 (red, catalog number 333620) and CD4 (blue, catalog number 300519) | |:-------:| | ![](../CD163_AF647/Human_LN_CD163_CD4.jpg) | + + +| Human normal LN: BCL2 (cyan, catalog number ab236221 and A-31573), CD3 (magenta, catalog number ab208514), CD4 (blue, catalog number ab196372), CD21 (yellow, catalog number ab306325) | +|:-------:| +| ![](../BCL2_Unconjugated/Human_normal_LN_BCL2_ab236221_CD3_ab208514_CD4_ab196372_CD21_ab306325.jpg) | + + +| Human FL LN: BCL2 (cyan, catalog number ab236221 and A-31573), CD3 (magenta, catalog number ab208514), CD4 (blue, catalog number ab196372), CD21 (yellow, catalog number ab306325) | +|:-------:| +| ![](../BCL2_Unconjugated/Human_FL_LN_BCL2_ab236221_CD3_ab208514_CD4_ab196372_CD21_ab306325.jpg) |