diff --git a/.zenodo.json b/.zenodo.json index 744f0bc..1b1e083 100644 --- a/.zenodo.json +++ b/.zenodo.json @@ -112,6 +112,11 @@ "name": "Koenig, Joshua F. E.", "orcid": "0000-0002-8909-5039" }, + { + "affiliation": "National Institutes of Health", + "name": "Makranz, Chen", + "orcid": "0000-0002-6944-6997" + }, { "affiliation": "McMaster University", "name": "Murakami Olyntho, Vitoria", diff --git a/data/reagent_resources.csv b/data/reagent_resources.csv index e5a3801..c1c8638 100644 --- a/data/reagent_resources.csv +++ b/data/reagent_resources.csv @@ -1062,3 +1062,14 @@ P49840,Primary Antibody,Phospho-GSK-3alpha,Human,Rabbit,IgG,36E9,Cell Signaling Q9Y5Y7,Primary Antibody,LYVE-1,Human,Goat,IgG,Polyclonal,R&D Systems,AF2089,Unconjugated,AB_355144,Stock,Multiplexed 2D Imaging,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P08575,Primary Antibody,CD45,Human,Mouse,IgG1,2B11 + PD7/26,Novus Biologicals,NBP2-34528AF700,AF700,NA,Stock,Multiplexed 2D Imaging,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q6FHL8,Primary Antibody,CTGF,Human,Rabbit,IgG,OT11F4,FabGennix,CTGF-FITC,FITC,NA,Stock,Multiplexed 2D Imaging,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967 +E9PVX6,Primary Antibody,Ki-67,Mouse,Rat,IgG2a,SolA15,Thermo Fisher Scientific,606-5698-82,AF660,AB_2896286,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997 +Q08481,Primary Antibody,CD31,Mouse,Rat,IgG2a,MEC13.3,BioLegend,102515,AF647,AB_2161030,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997 +P06332,Primary Antibody,CD4,Mouse,Rat,IgG2a,RM4-5,BioLegend,100533,AF647,AB_493372,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997 +P06332,Primary Antibody,CD4,Mouse,Rat,IgG2a,RM4-5,BioLegend,100533,AF647,AB_493372,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Spleen,Healthy,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997 +NA,Primary Antibody,mCherrry,Mouse,Chicken,IgY,polyclonal,Novus Biologicals,NBP2-25158,NA,AB_2636881,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997 +Q9EQX4,Primary Antibody,Iba-1,Mouse,Rabbit,IgG,polyclonal,FUJIFILM Wako Chemicals U.S.A. Corporation,019-19741,NA,AB_839504,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997 +P63158,Primary Antibody,CXCL12,Mouse,Rabbit,IgG,polyclonal,Abcam,AB25117,NA,AB_2088164,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997 +P70658,Primary Antibody,CXCR4,Mouse,Rabbit,IgG,polyclonal,Novus Biologicals,NB-100 74396,NA,AB_1048484,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997 +P56485,Primary Antibody,CXCR7,Mouse,Rabbit,IgG,polyclonal,Novus Biologicals,NBP2-24779G,DyLight 488,NA,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997 +Q9QXH4,Primary Antibody,CD11c,Mouse,Armenian Hamster,IgG,N418,BioLegend,117314,AF647,AB_492850,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997 +P03995,Primary Antibody,GFAP,Mouse,Mouse,IgG1,GA5,Thermo Fisher Scientific,41-9892-82,eF570,AB_2573656,Stock,Multiplexed 2D Imaging,4% PFA Fixed Frozen,Brain,Tumor,0.3% Triton-X-100,NA,NA,Yes,0000-0002-6944-6997,NA,0000-0002-6944-6997 diff --git a/data/vendor_urls.csv b/data/vendor_urls.csv index 99ea0dc..0803d29 100644 --- a/data/vendor_urls.csv +++ b/data/vendor_urls.csv @@ -26,4 +26,6 @@ IBT BioServices,https://www.ibtbioservices.com/ Cell IDx,https://cellidx.com/ Vector Laboratories,https://vectorlabs.com/ Agilent,https://www.agilent.com/en/dako-products -FabGennix,https://fabgennix.com/ \ No newline at end of file +FabGennix,https://fabgennix.com/ +Synaptic Systems,https://www.sysy.com/ +FUJIFILM Wako Chemicals U.S.A. Corporation,https://labchem-wako.fujifilm.com diff --git a/docs/supporting_material/CD11c_AF647/0000-0002-6944-6997.md b/docs/supporting_material/CD11c_AF647/0000-0002-6944-6997.md new file mode 100644 index 0000000..b0e0536 --- /dev/null +++ b/docs/supporting_material/CD11c_AF647/0000-0002-6944-6997.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:-----------------|:----------|:------------|:----------|-----------------:|:------------|:----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| +| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117314 | AF647 | AB_492850 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | + +# Publications + + + +# Additional Notes + + +1. Dilution 1:25. + +| Mouse Spleen Hochest (Blue) CD11c (Magenta Biolegend #117314) | +|:-------:| +| ![](Mouse_Spleen_Hochest_Blue_CD11c_Magenta_Biolegend_117314.jpg) | diff --git a/docs/supporting_material/CD11c_AF647/Mouse_Spleen_Hochest_Blue_CD11c_Magenta_Biolegend_117314.jpg b/docs/supporting_material/CD11c_AF647/Mouse_Spleen_Hochest_Blue_CD11c_Magenta_Biolegend_117314.jpg new file mode 100644 index 0000000..acd845e Binary files /dev/null and b/docs/supporting_material/CD11c_AF647/Mouse_Spleen_Hochest_Blue_CD11c_Magenta_Biolegend_117314.jpg differ diff --git a/docs/supporting_material/CD31_AF647/0000-0002-6944-6997.md b/docs/supporting_material/CD31_AF647/0000-0002-6944-6997.md new file mode 100644 index 0000000..2cdfd50 --- /dev/null +++ b/docs/supporting_material/CD31_AF647/0000-0002-6944-6997.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| +| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102515 | AF647 | AB_2161030 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | + +# Publications + + + +# Additional Notes + + +1. Dilution 1:100. + +| Mouse Brain Tumor Hochest(Blue) mCherry (Green Novus #NBP2-25158) CD31(Magenta Biolegend #102515) | +|:-------:| +| ![](Mouse_Brain_Tumor_Hochest_Blue_mCherry_Green_Novus_NBP2_25158_CD31_Magenta_Biolegend_102515.jpg) | diff --git a/docs/supporting_material/CD31_AF647/Mouse_Brain_Tumor_Hochest_Blue_mCherry_Green_Novus_NBP2_25158_CD31_Magenta_Biolegend_102515.jpg b/docs/supporting_material/CD31_AF647/Mouse_Brain_Tumor_Hochest_Blue_mCherry_Green_Novus_NBP2_25158_CD31_Magenta_Biolegend_102515.jpg new file mode 100644 index 0000000..39a8482 Binary files /dev/null and b/docs/supporting_material/CD31_AF647/Mouse_Brain_Tumor_Hochest_Blue_mCherry_Green_Novus_NBP2_25158_CD31_Magenta_Biolegend_102515.jpg differ diff --git a/docs/supporting_material/CD4_AF647/0000-0002-6944-6997.md b/docs/supporting_material/CD4_AF647/0000-0002-6944-6997.md new file mode 100644 index 0000000..bd8cbb0 --- /dev/null +++ b/docs/supporting_material/CD4_AF647/0000-0002-6944-6997.md @@ -0,0 +1,28 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| +| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BioLegend | 100533 | AF647 | AB_493372 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | +| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BioLegend | 100533 | AF647 | AB_493372 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Spleen | Healthy | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | + +# Publications + + + +# Additional Notes + + +1. Dilution 1:50. + +| Mouse Brain Tumor Hochest (Blue) mCherry (green, Novus #NBP2-25158 ) CD4 (Magenta, Biolegend #100533) | +|:-------:| +| ![](Mouse_Tumor_Brain_Hochest_Blue_mCherry_green_Novus_NBP2_25158_CD4_Magenta_Biolegend_100533.jpg) | + + +| Mouse Spleen Hochest (Blue) CD4 (Magenta Biolegend #100533) | +|:-------:| +| ![](Mouse_Spleen_Hochest_Blue_CD4_Magenta_Biolegend_100533.jpg) | diff --git a/docs/supporting_material/CD4_AF647/Mouse_Spleen_Hochest_Blue_CD4_Magenta_Biolegend_100533.jpg b/docs/supporting_material/CD4_AF647/Mouse_Spleen_Hochest_Blue_CD4_Magenta_Biolegend_100533.jpg new file mode 100644 index 0000000..60b4839 Binary files /dev/null and b/docs/supporting_material/CD4_AF647/Mouse_Spleen_Hochest_Blue_CD4_Magenta_Biolegend_100533.jpg differ diff --git a/docs/supporting_material/CD4_AF647/Mouse_Tumor_Brain_Hochest_Blue_mCherry_green_Novus_NBP2_25158_CD4_Magenta_Biolegend_100533.jpg b/docs/supporting_material/CD4_AF647/Mouse_Tumor_Brain_Hochest_Blue_mCherry_green_Novus_NBP2_25158_CD4_Magenta_Biolegend_100533.jpg new file mode 100644 index 0000000..5ea37d8 Binary files /dev/null and b/docs/supporting_material/CD4_AF647/Mouse_Tumor_Brain_Hochest_Blue_mCherry_green_Novus_NBP2_25158_CD4_Magenta_Biolegend_100533.jpg differ diff --git a/docs/supporting_material/CXCL12_NA/0000-0002-6944-6997.md b/docs/supporting_material/CXCL12_NA/0000-0002-6944-6997.md new file mode 100644 index 0000000..f7f2bfc --- /dev/null +++ b/docs/supporting_material/CXCL12_NA/0000-0002-6944-6997.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------|:-----------------|:------------|:-----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| +| P63158 | Primary Antibody | CXCL12 | Mouse | Rabbit | IgG | polyclonal | Abcam | AB25117 | NA | AB_2088164 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | + +# Publications + + + +# Additional Notes + + +1. Dilution 1:100. + +| Mouse Brain Tumor Hochest (Blue) CXCL12 (Green Abcam #AB25117) mCherry (Magenta, Novus #NBP2-25158) | +|:-------:| +| ![](Mouse_Tumor_Brain_Hochest_Blue_CXCL12_Green_Abcam_AB25117_mCherry_Magenta_Novus_NBP2_25158.jpg) | diff --git a/docs/supporting_material/CXCL12_NA/Mouse_Tumor_Brain_Hochest_Blue_CXCL12_Green_Abcam_AB25117_mCherry_Magenta_Novus_NBP2_25158.jpg b/docs/supporting_material/CXCL12_NA/Mouse_Tumor_Brain_Hochest_Blue_CXCL12_Green_Abcam_AB25117_mCherry_Magenta_Novus_NBP2_25158.jpg new file mode 100644 index 0000000..3cce098 Binary files /dev/null and b/docs/supporting_material/CXCL12_NA/Mouse_Tumor_Brain_Hochest_Blue_CXCL12_Green_Abcam_AB25117_mCherry_Magenta_Novus_NBP2_25158.jpg differ diff --git a/docs/supporting_material/CXCR4_NA/0000-0002-6944-6997.md b/docs/supporting_material/CXCR4_NA/0000-0002-6944-6997.md new file mode 100644 index 0000000..8353b77 --- /dev/null +++ b/docs/supporting_material/CXCR4_NA/0000-0002-6944-6997.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:------------------|:-----------------|:------------|:-----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| +| P70658 | Primary Antibody | CXCR4 | Mouse | Rabbit | IgG | polyclonal | Novus Biologicals | NB-100 74396 | NA | AB_1048484 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | + +# Publications + + + +# Additional Notes + + +1. Dilution 1:200. + +| Mouse Brain Tumor Hochest (Blue) CXCR4 (Green Novus # NB-100 74396) | +|:-------:| +| ![](Mouse_Brain_Tumor_Hochest_Blue_CXCR4_Green_Novus_NB_100_74396.jpg) | diff --git a/docs/supporting_material/CXCR4_NA/Mouse_Brain_Tumor_Hochest_Blue_CXCR4_Green_Novus_NB_100_74396.jpg b/docs/supporting_material/CXCR4_NA/Mouse_Brain_Tumor_Hochest_Blue_CXCR4_Green_Novus_NB_100_74396.jpg new file mode 100644 index 0000000..0deb0f8 Binary files /dev/null and b/docs/supporting_material/CXCR4_NA/Mouse_Brain_Tumor_Hochest_Blue_CXCR4_Green_Novus_NB_100_74396.jpg differ diff --git a/docs/supporting_material/CXCR7_DyLight_488/0000-0002-6944-6997.md b/docs/supporting_material/CXCR7_DyLight_488/0000-0002-6944-6997.md new file mode 100644 index 0000000..bf0503b --- /dev/null +++ b/docs/supporting_material/CXCR7_DyLight_488/0000-0002-6944-6997.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:------------------|:-----------------|:------------|:-------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| +| P56485 | Primary Antibody | CXCR7 | Mouse | Rabbit | IgG | polyclonal | Novus Biologicals | NBP2-24779G | DyLight 488 | NA | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | + +# Publications + + + +# Additional Notes + + +1. Dilution 1:100. + +| Mouse Brain Tumor Hochest (Blue) CXCR7 (Green Novus #NBP2-24779G) | +|:-------:| +| ![](Mouse_Brain_Tumor_Hochest_Blue_CXCR7_Green_Novus_NBP2_24779G.jpg) | diff --git a/docs/supporting_material/CXCR7_DyLight_488/Mouse_Brain_Tumor_Hochest_Blue_CXCR7_Green_Novus_NBP2_24779G.jpg b/docs/supporting_material/CXCR7_DyLight_488/Mouse_Brain_Tumor_Hochest_Blue_CXCR7_Green_Novus_NBP2_24779G.jpg new file mode 100644 index 0000000..595ead3 Binary files /dev/null and b/docs/supporting_material/CXCR7_DyLight_488/Mouse_Brain_Tumor_Hochest_Blue_CXCR7_Green_Novus_NBP2_24779G.jpg differ diff --git a/docs/supporting_material/GFAP_eF570/0000-0002-6944-6997.md b/docs/supporting_material/GFAP_eF570/0000-0002-6944-6997.md new file mode 100644 index 0000000..4be00b7 --- /dev/null +++ b/docs/supporting_material/GFAP_eF570/0000-0002-6944-6997.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:-----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| +| P03995 | Primary Antibody | GFAP | Mouse | Mouse | IgG1 | GA5 | Thermo Fisher Scientific | 41-9892-82 | eF570 | AB_2573656 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | + +# Publications + + + +# Additional Notes + + +1. Dilution 1:50. + +| Mouse Brain Tumor Hochest (Cyan) GFAP (Orange Thermo #41-9892-82) | +|:-------:| +| ![](Mouse_Brain_Tumor_Hochest_cyan_GFAP_Orange_Thermo_41_9892_82.jpg) | diff --git a/docs/supporting_material/GFAP_eF570/Mouse_Brain_Tumor_Hochest_cyan_GFAP_Orange_Thermo_41_9892_82.jpg b/docs/supporting_material/GFAP_eF570/Mouse_Brain_Tumor_Hochest_cyan_GFAP_Orange_Thermo_41_9892_82.jpg new file mode 100644 index 0000000..5a99223 Binary files /dev/null and b/docs/supporting_material/GFAP_eF570/Mouse_Brain_Tumor_Hochest_cyan_GFAP_Orange_Thermo_41_9892_82.jpg differ diff --git a/docs/supporting_material/Iba-1_NA/0000-0002-6944-6997.md b/docs/supporting_material/Iba-1_NA/0000-0002-6944-6997.md new file mode 100644 index 0000000..3b8e3b3 --- /dev/null +++ b/docs/supporting_material/Iba-1_NA/0000-0002-6944-6997.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:------------------------|:-----------------|:------------|:----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| +| Q9EQX4 | Primary Antibody | Iba-1 | Mouse | Rabbit | IgG | polyclonal | FUJIFILM Wako Chemicals U.S.A. Corporation | 019-19741 | NA | AB_839504 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | + +# Publications + + + +# Additional Notes + + +1. Dilution 1:500. + +| Mouse Brain Tumor Hochest (Cyan) Iba (Green_Fuji #019-19741) | +|:-------:| +| ![](Mouse_Brain_Tumor_Hochest_Cyan_Iba_Green_Fuji_019-19741.jpg) | diff --git a/docs/supporting_material/Iba-1_NA/Mouse_Brain_Tumor_Hochest_Cyan_Iba_Green_Fuji_019-19741.jpg b/docs/supporting_material/Iba-1_NA/Mouse_Brain_Tumor_Hochest_Cyan_Iba_Green_Fuji_019-19741.jpg new file mode 100644 index 0000000..a2a5d5a Binary files /dev/null and b/docs/supporting_material/Iba-1_NA/Mouse_Brain_Tumor_Hochest_Cyan_Iba_Green_Fuji_019-19741.jpg differ diff --git a/docs/supporting_material/Ki-67_AF660/0000-0002-6944-6997.md b/docs/supporting_material/Ki-67_AF660/0000-0002-6944-6997.md new file mode 100644 index 0000000..7939bf4 --- /dev/null +++ b/docs/supporting_material/Ki-67_AF660/0000-0002-6944-6997.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:-----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| +| E9PVX6 | Primary Antibody | Ki-67 | Mouse | Rat | IgG2a | SolA15 | Thermo Fisher Scientific | 606-5698-82 | AF660 | AB_2896286 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | + +# Publications + + + +# Additional Notes + + +1. Dilution 1:100. + +| Mouse Brain Tumor Hochest (Blue) Iba (Cyan, Fuji #019-19741) mCherry (Red, Novus #NBP2-25158) Ki67 (Magenta Thermo #606-5698-82) | +|:-------:| +| ![](Mouse_Brain_Tumor_Hochest_Blue_Iba_Cyan_Fuji_019_19741_mCherry_Red_Novus_NBP2_25158_Ki67_Magenta_Thermo_606_5698_82.jpg) | diff --git a/docs/supporting_material/Ki-67_AF660/Mouse_Brain_Tumor_Hochest_Blue_Iba_Cyan_Fuji_019_19741_mCherry_Red_Novus_NBP2_25158_Ki67_Magenta_Thermo_606_5698_82.jpg b/docs/supporting_material/Ki-67_AF660/Mouse_Brain_Tumor_Hochest_Blue_Iba_Cyan_Fuji_019_19741_mCherry_Red_Novus_NBP2_25158_Ki67_Magenta_Thermo_606_5698_82.jpg new file mode 100644 index 0000000..369c0f9 Binary files /dev/null and b/docs/supporting_material/Ki-67_AF660/Mouse_Brain_Tumor_Hochest_Blue_Iba_Cyan_Fuji_019_19741_mCherry_Red_Novus_NBP2_25158_Ki67_Magenta_Thermo_606_5698_82.jpg differ diff --git a/docs/supporting_material/mCherrry_NA/0000-0002-6944-6997.md b/docs/supporting_material/mCherrry_NA/0000-0002-6944-6997.md new file mode 100644 index 0000000..c46b452 --- /dev/null +++ b/docs/supporting_material/mCherrry_NA/0000-0002-6944-6997.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:------------------|:-----------------|:------------|:-----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:-------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| +| NA | Primary Antibody | mCherrry | Mouse | Chicken | IgY | polyclonal | Novus Biologicals | NBP2-25158 | NA | AB_2636881 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | NA | [0000-0002-6944-6997](https://orcid.org/0000-0002-6944-6997) | [1](#notes) | + +# Publications + + + +# Additional Notes + + +1. Dilution 1:2000. Not specific (stains also brain tissue with no mCherry - tumor cells). + +| Mouse Brain Tumor Hochest (Cyan) mcherry (red Novus #NBP2-25158) | +|:-------:| +| ![](Mouse_Brain_Tumor_Hochest_Cyan_mcherry_red_Novus_NBP2_25158.jpg) | diff --git a/docs/supporting_material/mCherrry_NA/Mouse_Brain_Tumor_Hochest_Cyan_mcherry_red_Novus_NBP2_25158.jpg b/docs/supporting_material/mCherrry_NA/Mouse_Brain_Tumor_Hochest_Cyan_mcherry_red_Novus_NBP2_25158.jpg new file mode 100644 index 0000000..05db38a Binary files /dev/null and b/docs/supporting_material/mCherrry_NA/Mouse_Brain_Tumor_Hochest_Cyan_mcherry_red_Novus_NBP2_25158.jpg differ