diff --git a/.zenodo.json b/.zenodo.json index 0e4b70a..e090f7c 100644 --- a/.zenodo.json +++ b/.zenodo.json @@ -97,6 +97,11 @@ "name": "Ichise, Hiroshi", "orcid": "0000-0002-5187-810X" }, + { + "affiliation": "National Institutes of Health", + "name": "Jing, Zhixin", + "orcid": "0000-0003-1118-7432" + }, { "affiliation": "University Hospital Aachen", "name": "Jonigk, Danny", diff --git a/data/image_resources.csv b/data/image_resources.csv index 9746ad2..2a79a9b 100644 --- a/data/image_resources.csv +++ b/data/image_resources.csv @@ -153,3 +153,7 @@ Phospho_NF-Kappa_B_p105_Unconjugated/FL_FFPE_PhosphoNF-KappaBp105_CST_4808.jpg," Iba-1_NA/Mouse_Brain_Tumor_Hochest_Cyan_Iba_Green_Fuji_019-19741.jpg,Mouse Brain Tumor Hochest (Cyan) Iba (Green_Fuji #019-19741),e243fceacf9de423e29bc97537e19af2,['Iba-1_NA/0000-0002-6944-6997.md'] CD4_AF532/CD4_AF532_Thermo_58-0049-42_CD8_AF488_BioLegend_344716.jpg,"Human lymph node: CD4 (cyan, catalog number 58-0049-42) and CD8 (red, catalog number 561525) ",e56f1f54bbd49e429eba4c2cf0b8a9fe,"['CD8_AF488/0000-0003-4379-8967.md', 'CD4_AF532/0000-0003-4379-8967.md']" CD4_AF700/Human_Metastatic_Lymph_Node_CD4_EpCAM.jpg,"Human lymph node metastatic: CD4 (blue, catalog number 300526) and EpCAM (red, catalog number 324228)",3f0b3658cc378384708e5224782c30db,"['EpCAM_AF594/0000-0003-4379-8967.md', 'CD4_AF700/0000-0003-4379-8967.md']" +B220_AF488/Mouse_LN_B220_cyan_BD_557669_CD3_red_BioLegend_100240.jpg,"Mouse lymph node: B220 (cyan, catalog number 557669) and CD3 (red, catalog number 100240)",70ba1f6cb266c846c2be414648e31960,"['B220_AF488/0000-0003-1118-7432.md','CD3_AF594/0000-0003-1118-7432.md']" +CD35_BV510/Mouse_LN_CD35_yellow_BD_740132_ColIV_blue_ab19808_A-11009.jpg,"Mouse lymph node: CD35 (yellow, catalog number 740132) and Collagen IV (blue, catalog number ab19808 and A-11009)",ceb4fd8cd2cc42201659aa8226b9f07a,"['CD35_BV510/0000-0003-1118-7432.md','Collagen_IV_Unconjugated/0000-0003-1118-7432.md','Rabbit_IgG__H+L__AF532/0000-0003-1118-7432.md']" +CD169_PE/Mouse_LN_CD169_green_BioLegend_142404_Ki67_magenta_BD_561277.jpg,"Mouse lymph node: CD169 (green, catalog number 142404) and Ki-67 (magenta, catalog number 561277)",8a6567cde9020c3f7cf4baa8a32666f2,"['Ki-67_AF700/0000-0003-1118-7432.md','CD169_PE/0000-0003-1118-7432.md']" +PD-1_BV421/Mouse_LN_PD1_cyan_135218_CD11c_magenta_117312.jpg,"Mouse lymph node: PD-1 (cyan, catalog number 135218) and CD11c (magenta, catalog number 117312)",28a7b8aa0b9b5764fe0061fb69312229,"['CD11c_AF647/0000-0003-1118-7432.md','PD-1_BV421/0000-0003-1118-7432.md']" \ No newline at end of file diff --git a/data/reagent_resources.csv b/data/reagent_resources.csv index 700ddd0..6668fb1 100644 --- a/data/reagent_resources.csv +++ b/data/reagent_resources.csv @@ -1,4 +1,4 @@ -UniProt Accession Number,Reagent Type,Target Name / Protein Biomarker,Target Species,Host Organism,Isotype,Clonality,Vendor,Catalog Number,Conjugate,RRID,Availability,Method,Tissue Preservation,Target Tissue,Tissue State,Detergent,Antigen Retrieval Conditions,Dye Inactivation Conditions,Recommend,Agree,Disagree,Contributor +UniProt Accession Number,Reagent Type,Target Name / Protein Biomarker,Target Species,Host Organism,Isotype,Clonality,Vendor,Catalog Number,Conjugate,RRID,Availability,Method,Tissue Preservation,Target Tissue,Tissue State,Detergent,Antigen Retrieval Conditions,Dye Inactivation Conditions,Recommend,Agree,Disagree,Contributor P16422,Primary Antibody,EpCAM,Human,Mouse,IgG2b,9C4,BioLegend,324210,AF488,AB_756084,Stock,IBEX2D Automated,1:4 Cytofix/Cytoperm Fixed Frozen,Jejunum,NA,0.3% Triton-X-100,NA,0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P19320,Primary Antibody,CD106,Human,Mouse,IgG1,STA,BioLegend,305806,PE,AB_314561,Stock,IBEX2D Automated,1:4 Cytofix/Cytoperm Fixed Frozen,Jejunum,NA,0.3% Triton-X-100,NA,0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P19320,Primary Antibody,CD106,Human,Mouse,IgG1,STA,BioLegend,305806,PE,AB_314561,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 @@ -220,9 +220,9 @@ P02462,Primary Antibody,Collagen IV,Mouse,Rabbit,IgG,Polyclonal,Abcam,ab19808,Un NA,Secondary Antibody,Rabbit IgG (H+L),Mouse,Goat,IgG,Polyclonal,Thermo Fisher Scientific,A-21038,AF700,AB_2535709,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P02462,Primary Antibody,Collagen IV,Mouse,Rabbit,IgG,Polyclonal,Abcam,ab19808,Unconjugated,AB_445160,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lung,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 NA,Secondary Antibody,Rabbit IgG (H+L),Mouse,Goat,IgG,Polyclonal,Thermo Fisher Scientific,A-21038,AF700,AB_2535709,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lung,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -P02462,Primary Antibody,Collagen IV,Mouse,Rabbit,IgG,Polyclonal,Abcam,ab19808,Unconjugated,AB_445160,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P02462,Primary Antibody,Collagen IV,Mouse,Rabbit,IgG,Polyclonal,Abcam,ab19808,Unconjugated,AB_445160,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0003-4379-8967;0000-0003-1118-7432,NA,0000-0003-4379-8967 NA,Secondary Antibody,Rabbit IgG (H+L),Mouse,Goat,IgG,Polyclonal,Thermo Fisher Scientific,A-21038,AF700,AB_2535709,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -Q62230,Primary Antibody,CD169,Mouse,Rat,IgG2a,3D6.112,BioLegend,142404,PE,AB_10915697,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +Q62230,Primary Antibody,CD169,Mouse,Rat,IgG2a,3D6.112,BioLegend,142404,PE,AB_10915697,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967;0000-0003-1118-7432,NA,0000-0003-4379-8967 Q62230,Primary Antibody,CD169,Mouse,Rat,IgG2a,3D6.112,BioLegend,142404,PE,AB_10915697,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lung,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q62230,Primary Antibody,CD169,Mouse,Rat,IgG2a,3D6.112,BioLegend,142404,PE,AB_10915697,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Spleen,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q62230,Primary Antibody,CD169,Mouse,Rat,IgG2a,3D6.112,BioLegend,142404,PE,AB_10915697,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 @@ -231,7 +231,7 @@ Q9QXH4,Primary Antibody,CD11c,Mouse,Armenian Hamster,IgG,N418,BioLegend,117312,A Q9QXH4,Primary Antibody,CD11c,Mouse,Armenian Hamster,IgG,N418,BioLegend,117312,AF647,AB_389328,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lung,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q9QXH4,Primary Antibody,CD11c,Mouse,Armenian Hamster,IgG,N418,BioLegend,117312,AF647,AB_389328,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Small Intestine,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q9QXH4,Primary Antibody,CD11c,Mouse,Armenian Hamster,IgG,N418,BioLegend,117312,AF647,AB_389328,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Liver,NA,NA,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-1431-1398,NA,0000-0003-1431-1398 -Q9QXH4,Primary Antibody,CD11c,Mouse,Armenian Hamster,IgG,N418,BioLegend,117312,AF647,AB_389328,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +Q9QXH4,Primary Antibody,CD11c,Mouse,Armenian Hamster,IgG,N418,BioLegend,117312,AF647,AB_389328,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967;0000-0003-1118-7432,NA,0000-0003-4379-8967 NA,Primary Antibody,MHC-II,Mouse,Rat,IgG2b,M5/114.15.2,BioLegend,107622,AF700,AB_493727,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Spleen,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 NA,Primary Antibody,MHC-II,Mouse,Rat,IgG2b,M5/114.15.2,BioLegend,107622,AF700,AB_493727,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 NA,Primary Antibody,MHC-II,Mouse,Rat,IgG2b,M5/114.15.2,BioLegend,107622,AF700,AB_493727,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lung,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 @@ -260,7 +260,7 @@ P22646,Primary Antibody,CD3,Mouse,Rat,IgG2b,17A2,BD Biosciences,557869,AF647,AB_ E9PVX6,Primary Antibody,Ki-67,Mouse,Mouse,IgG1,B56,BD Biosciences,561277,AF700,AB_10611571,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Spleen,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 E9PVX7,Primary Antibody,Ki-67,Mouse,Mouse,IgG1,B56,BD Biosciences,561277,AF700,AB_10611571,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 E9PVX8,Primary Antibody,Ki-67,Mouse,Mouse,IgG1,B56,BD Biosciences,561277,AF700,AB_10611571,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Small Intestine,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -E9PVX9,Primary Antibody,Ki-67,Mouse,Mouse,IgG1,B56,BD Biosciences,561277,AF700,AB_10611571,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +E9PVX9,Primary Antibody,Ki-67,Mouse,Mouse,IgG1,B56,BD Biosciences,561277,AF700,AB_10611571,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967;0000-0003-1118-7432,NA,0000-0003-4379-8967 P18828,Primary Antibody,CD138,Mouse,Rat,IgG2a,281-2,BD Biosciences,562610,BV421,AB_11153126,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P18828,Primary Antibody,CD138,Mouse,Rat,IgG2a,281-2,BD Biosciences,562610,BV421,AB_11153126,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lung,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P18828,Primary Antibody,CD138,Mouse,Rat,IgG2a,281-2,BD Biosciences,562610,BV421,AB_11153126,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 @@ -270,7 +270,7 @@ P24788,Primary Antibody,CD11b,Mouse,Rat,IgG2b,5C6,Bio-Rad,MCA711F,FITC,AB_110061 P24788,Primary Antibody,CD11b,Mouse,Rat,IgG2b,5C6,Bio-Rad,MCA711F,FITC,AB_1100617,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 30 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q9ERD7,Primary Antibody,Tubulin beta-3 chain,Mouse,Mouse,IgG2a,AA10,BioLegend,657402 (Unconjugated),AF532,NA,Custom,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P22646,Primary Antibody,CD3,Mouse,Rat,IgG2b,17A2,BioLegend,100240,AF594,AB_2563427,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 for more than 120 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -P22646,Primary Antibody,CD3,Mouse,Rat,IgG2b,17A2,BioLegend,100240,AF594,AB_2563427,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 for more than 120 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P22646,Primary Antibody,CD3,Mouse,Rat,IgG2b,17A2,BioLegend,100240,AF594,AB_2563427,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 for more than 120 minutes,Yes,0000-0003-4379-8967;0000-0003-1118-7432,NA,0000-0003-4379-8967 P29533,Primary Antibody,CD106,Mouse,Rat,IgG2a,429,Thermo Fisher Scientific,50-1061-82,eF660,AB_11219864,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P01590,Primary Antibody,CD25,Mouse,Rat,IgG1,PC61.5,Thermo Fisher Scientific,53-0251-82,AF488,AB_763472,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q99JB6,Primary Antibody,FOXP3,Mouse,Rat,IgG2a,FJK-16s,Thermo Fisher Scientific,58-5773-82,AF532,AB_11218870,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 @@ -329,9 +329,9 @@ Q6U7R4,Primary Antibody,Tim-4,Mouse,Rat,IgG1,21H12,BD Biosciences,742773,BV421,A P24788,Primary Antibody,CD11b,Mouse,Rat,IgG2b,M1/70,BioLegend,101217,AF488,AB_389305,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Liver,NA,NA,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-1431-1398,NA,0000-0003-1431-1398 P15104,Primary Antibody,Glutamine synthetase,Mouse,Rabbit,IgG,Polyclonal,Abcam,ab49873,Unconjugated,AB_880241,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Liver,NA,NA,NA,NA,Yes,0000-0003-1431-1398,NA,0000-0003-1431-1398 P11609,Primary Antibody,CD1d,Mouse,Rat,IgG2b,1B1,BD Biosciences,553846,PE,AB_2073521,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Liver,NA,NA,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-1431-1398,NA,0000-0003-1431-1398 -Q02242,Primary Antibody,PD-1,Mouse,Rat,IgG2a,29F.1.A12,BioLegend,135218,BV421,AB_2561447,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -NA,Primary Antibody,CD35,Mouse,Rat,IgG2a,8C12,BD Biosciences,740132,BV510,AB_2739889,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -NA,Primary Antibody,B220,Mouse,Rat,IgG2a,RA3-6B2,BD Biosciences,557669,AF488,AB_396781,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +Q02242,Primary Antibody,PD-1,Mouse,Rat,IgG2a,29F.1.A12,BioLegend,135218,BV421,AB_2561447,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967;0000-0003-1118-7432,NA,0000-0003-4379-8967 +NA,Primary Antibody,CD35,Mouse,Rat,IgG2a,8C12,BD Biosciences,740132,BV510,AB_2739889,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967;0000-0003-1118-7432,NA,0000-0003-4379-8967 +NA,Primary Antibody,B220,Mouse,Rat,IgG2a,RA3-6B2,BD Biosciences,557669,AF488,AB_396781,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967;0000-0003-1118-7432,NA,0000-0003-4379-8967 P41183,Primary Antibody,BCL6,Mouse,Mouse,IgG1,K112-91,BD Biosciences,561525,AF647,AB_10898007,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q9ES57,Primary Antibody,CD200,Mouse,Rat,IgG2a,OX-90,BD Biosciences,565547,BV421,AB_2739289,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes + light,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q62011,Primary Antibody,gp38,Mouse,Syrian Hamster,IgG,8.1.1,BioLegend,127401,Unconjugated,AB_1089186,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 @@ -528,7 +528,7 @@ P08138,Primary Antibody,CD271 (NGFR5),Human,Mouse,IgG1,NGFR5,Thermo Fisher Scien P10645,Primary Antibody,Chromogranin A,Human,Mouse,IgG1,LK2H10,Novus Biologicals,NBP2-34672AF532,AF532,AB_2892553,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Pancreas,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0002-3882-457X,NA,0000-0002-3882-457X Q9BWP8,Primary Antibody,COLEC11,Human,Rabbit,IgG,Polyclonal,Proteintech,15269-1-AP,Unconjugated,AB_10732940,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,NA,No,0000-0002-3882-457X,NA,0000-0002-3882-457X Q6ICW0,Primary Antibody,CXCL12,Human,Mouse,IgG1,79018,R&D Systems,MAB350,Unconjugated,AB_2088149,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Liver,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -NA,Primary Antibody,Cytokeratin (Pan),Human,Mouse,IgG1,AE1/AE3,BioLegend,914204 (Unconjugated),AF532,NA,Custom,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0002-3882-457X,NA,0000-0002-3882-457X +NA,Primary Antibody,Cytokeratin (Pan),Human,Mouse,IgG1,AE1/AE3,BioLegend,"914204 (Unconjugated)",AF532,NA,Custom,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Thymus,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0002-3882-457X,NA,0000-0002-3882-457X NA,Primary Antibody,Cytokeratin (Pan),Human,Mouse,IgG1,AE1/AE3,Thermo Fisher Scientific,41-9003-80,eF570,AB_11217482,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 NA,Primary Antibody,Cytokeratin (Pan),Human,Mouse,IgG1,AE1/AE3,Thermo Fisher Scientific,42-9003-82,eF615,AB_10804663,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 NA,Primary Antibody,Cytokeratin (Pan),Human,Mouse,IgG1,AE1/AE3,Novus Biologicals,NBP2-33200AF750,AF750,AB_2868569,Stock,IBEX2D Manual,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML),1 mg/ml LiBH4 15 minutes,Yes,0000-0002-5187-810X,NA,0000-0002-5187-810X @@ -1095,3 +1095,4 @@ P62736,Primary Antibody,Alpha smooth muscle actin,Human,Mouse,IgG2a,1A4,Thermo F P60709,Cell Structure Reagent,Phalloidin,Human,NA,NA,NA,AAT Bioquest,23122,iF594,NA,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Retina,NA,0.1% Saponin,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-2088-8310,NA,0000-0003-2088-8310 NA,Lectin,PNA,Human,NA,NA,NA,Thermo Fisher Scientific,L21409,AF488,NA,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Retina,NA,0.1% Saponin,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-2088-8310,NA,0000-0003-2088-8310 NA,Lectin,Isolectin GS-IB4,Human,NA,NA,NA,Thermo Fisher Scientific,I21412,AF568,NA,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Retina,NA,0.1% Saponin,NA,NA,Yes,0000-0003-2088-8310,NA,0000-0003-2088-8310 +NA,Secondary Antibody,Rabbit IgG (H+L),Mouse,Goat,IgG,Polyclonal,Thermo Fisher Scientific,A-11009,AF532,AB_2534076,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-1118-7432,NA,0000-0003-1118-7432 \ No newline at end of file diff --git a/docs/supporting_material/B220_AF488/0000-0003-1118-7432.md b/docs/supporting_material/B220_AF488/0000-0003-1118-7432.md new file mode 100644 index 0000000..f64b399 --- /dev/null +++ b/docs/supporting_material/B220_AF488/0000-0003-1118-7432.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 557669 | AF488 | AB_396781 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: B220 (cyan, catalog number 557669) and CD3 (red, catalog number 100240) | +|:-------:| +| ![](Mouse_LN_B220_cyan_BD_557669_CD3_red_BioLegend_100240.jpg) | diff --git a/docs/supporting_material/B220_AF488/0000-0003-4379-8967.md b/docs/supporting_material/B220_AF488/0000-0003-4379-8967.md index 0829e8f..d3de876 100644 --- a/docs/supporting_material/B220_AF488/0000-0003-4379-8967.md +++ b/docs/supporting_material/B220_AF488/0000-0003-4379-8967.md @@ -6,7 +6,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:--------| -| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 557669 | AF488 | AB_396781 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 557669 | AF488 | AB_396781 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)]; [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | # Publications diff --git a/docs/supporting_material/B220_AF488/Mouse_LN_B220_cyan_BD_557669_CD3_red_BioLegend_100240.jpg b/docs/supporting_material/B220_AF488/Mouse_LN_B220_cyan_BD_557669_CD3_red_BioLegend_100240.jpg new file mode 100644 index 0000000..61ed282 Binary files /dev/null and b/docs/supporting_material/B220_AF488/Mouse_LN_B220_cyan_BD_557669_CD3_red_BioLegend_100240.jpg differ diff --git a/docs/supporting_material/CD11c_AF647/0000-0003-1118-7432.md b/docs/supporting_material/CD11c_AF647/0000-0003-1118-7432.md new file mode 100644 index 0000000..c0f7d04 --- /dev/null +++ b/docs/supporting_material/CD11c_AF647/0000-0003-1118-7432.md @@ -0,0 +1,21 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:-----------------|:----------|:------------|:----------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + +| Mouse lymph node: PD-1 (cyan, catalog number 135218) and CD11c (magenta, catalog number 117312) | +|:-------:| +| ![](../PD-1_BV421/Mouse_LN_PD1_cyan_135218_CD11c_magenta_117312.jpg) | diff --git a/docs/supporting_material/CD11c_AF647/0000-0003-4379-8967.md b/docs/supporting_material/CD11c_AF647/0000-0003-4379-8967.md index 5e551b6..421a8d6 100644 --- a/docs/supporting_material/CD11c_AF647/0000-0003-4379-8967.md +++ b/docs/supporting_material/CD11c_AF647/0000-0003-4379-8967.md @@ -10,7 +10,7 @@ layout: default | Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) | | Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | | Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | -| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) | +| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)]; [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) | # Publications diff --git a/docs/supporting_material/CD169_PE/0000-0003-1118-7432.md b/docs/supporting_material/CD169_PE/0000-0003-1118-7432.md new file mode 100644 index 0000000..ad73f33 --- /dev/null +++ b/docs/supporting_material/CD169_PE/0000-0003-1118-7432.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:------------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: CD169 (green, catalog number 142404) and Ki-67 (magenta, catalog number 561277) | +|:-------:| +| ![](Mouse_LN_CD169_green_BioLegend_142404_Ki67_magenta_BD_561277.jpg) | diff --git a/docs/supporting_material/CD169_PE/0000-0003-4379-8967.md b/docs/supporting_material/CD169_PE/0000-0003-4379-8967.md index 4e0d0da..571f48c 100644 --- a/docs/supporting_material/CD169_PE/0000-0003-4379-8967.md +++ b/docs/supporting_material/CD169_PE/0000-0003-4379-8967.md @@ -6,7 +6,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:------------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:------------| -| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)]; [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | | Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | | Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | | Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | diff --git a/docs/supporting_material/CD169_PE/Mouse_LN_CD169_green_BioLegend_142404_Ki67_magenta_BD_561277.jpg b/docs/supporting_material/CD169_PE/Mouse_LN_CD169_green_BioLegend_142404_Ki67_magenta_BD_561277.jpg new file mode 100644 index 0000000..b9cff4b Binary files /dev/null and b/docs/supporting_material/CD169_PE/Mouse_LN_CD169_green_BioLegend_142404_Ki67_magenta_BD_561277.jpg differ diff --git a/docs/supporting_material/CD35_BV510/0000-0003-1118-7432.md b/docs/supporting_material/CD35_BV510/0000-0003-1118-7432.md new file mode 100644 index 0000000..81dbefc --- /dev/null +++ b/docs/supporting_material/CD35_BV510/0000-0003-1118-7432.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | BD Biosciences | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: CD35 (yellow, catalog number 740132) and Collagen IV (blue, catalog number ab19808 and A-11009) | +|:-------:| +| ![](Mouse_LN_CD35_yellow_BD_740132_ColIV_blue_ab19808_A-11009.jpg) | diff --git a/docs/supporting_material/CD35_BV510/0000-0003-4379-8967.md b/docs/supporting_material/CD35_BV510/0000-0003-4379-8967.md index 51106c4..e6fffc4 100644 --- a/docs/supporting_material/CD35_BV510/0000-0003-4379-8967.md +++ b/docs/supporting_material/CD35_BV510/0000-0003-4379-8967.md @@ -7,7 +7,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:------------| | NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | BD Biosciences | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | -| NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | BD Biosciences | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | BD Biosciences | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)]; [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | # Publications diff --git a/docs/supporting_material/CD35_BV510/Mouse_LN_CD35_yellow_BD_740132_ColIV_blue_ab19808_A-11009.jpg b/docs/supporting_material/CD35_BV510/Mouse_LN_CD35_yellow_BD_740132_ColIV_blue_ab19808_A-11009.jpg new file mode 100644 index 0000000..7c64b10 Binary files /dev/null and b/docs/supporting_material/CD35_BV510/Mouse_LN_CD35_yellow_BD_740132_ColIV_blue_ab19808_A-11009.jpg differ diff --git a/docs/supporting_material/CD3_AF594/0000-0003-1118-7432.md b/docs/supporting_material/CD3_AF594/0000-0003-1118-7432.md new file mode 100644 index 0000000..52b89a2 --- /dev/null +++ b/docs/supporting_material/CD3_AF594/0000-0003-1118-7432.md @@ -0,0 +1,21 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:----------------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100240 | AF594 | AB_2563427 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + +| Mouse lymph node: B220 (cyan, catalog number 557669) and CD3 (red, catalog number 100240) | +|:-------:| +| ![](../B220_AF488/Mouse_LN_B220_cyan_BD_557669_CD3_red_BioLegend_100240.jpg) | diff --git a/docs/supporting_material/CD3_AF594/0000-0003-4379-8967.md b/docs/supporting_material/CD3_AF594/0000-0003-4379-8967.md index 2dbec07..38bf6fe 100644 --- a/docs/supporting_material/CD3_AF594/0000-0003-4379-8967.md +++ b/docs/supporting_material/CD3_AF594/0000-0003-4379-8967.md @@ -6,7 +6,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:--------------------|:------------------|:-------------------------------|:----------------------------------------|:------------|:-------------------------------------------------------------|:-----------|:--------------------|:------------| -| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300446 | AF594 | AB_2563236 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications), [1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | +| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300446 | AF594 | AB_2563236 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications), [1](#publications)]; [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | | P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100240 | AF594 | AB_2563427 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | | P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100240 | AF594 | AB_2563427 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | | P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300446 | AF594 | AB_2563236 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | diff --git a/docs/supporting_material/Collagen_IV_Unconjugated/0000-0003-1118-7432.md b/docs/supporting_material/Collagen_IV_Unconjugated/0000-0003-1118-7432.md new file mode 100644 index 0000000..e8deb3c --- /dev/null +++ b/docs/supporting_material/Collagen_IV_Unconjugated/0000-0003-1118-7432.md @@ -0,0 +1,21 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------|:-----------------|:-------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| P02462 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + +| Mouse lymph node: CD35 (yellow, catalog number 740132) and Collagen IV (blue, catalog number ab19808 and A-11009) | +|:-------:| +| ![](../CD35_BV510/Mouse_LN_CD35_yellow_BD_740132_ColIV_blue_ab19808_A-11009.jpg) | diff --git a/docs/supporting_material/Collagen_IV_Unconjugated/0000-0003-4379-8967.md b/docs/supporting_material/Collagen_IV_Unconjugated/0000-0003-4379-8967.md index 066e04f..06a7cdd 100644 --- a/docs/supporting_material/Collagen_IV_Unconjugated/0000-0003-4379-8967.md +++ b/docs/supporting_material/Collagen_IV_Unconjugated/0000-0003-4379-8967.md @@ -10,7 +10,7 @@ layout: default | P02462 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | | P02462 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | | P02462 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | -| P02462 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| P02462 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications)]; [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | # Publications diff --git a/docs/supporting_material/Ki-67_AF700/0000-0003-1118-7432.md b/docs/supporting_material/Ki-67_AF700/0000-0003-1118-7432.md new file mode 100644 index 0000000..f72608c --- /dev/null +++ b/docs/supporting_material/Ki-67_AF700/0000-0003-1118-7432.md @@ -0,0 +1,21 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:------------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| E9PVX9 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + +| Mouse lymph node: CD169 (green, catalog number 142404) and Ki-67 (magenta, catalog number 561277) | +|:-------:| +| ![](../CD169_PE/Mouse_LN_CD169_green_BioLegend_142404_Ki67_magenta_BD_561277.jpg) | diff --git a/docs/supporting_material/Ki-67_AF700/0000-0003-4379-8967.md b/docs/supporting_material/Ki-67_AF700/0000-0003-4379-8967.md index c27ed8c..1e23fba 100644 --- a/docs/supporting_material/Ki-67_AF700/0000-0003-4379-8967.md +++ b/docs/supporting_material/Ki-67_AF700/0000-0003-4379-8967.md @@ -12,7 +12,7 @@ layout: default | E9PVX6 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | | E9PVX7 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | | E9PVX8 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | -| E9PVX9 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| E9PVX9 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)]; [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | | Q9BYG3 | Primary Antibody | Ki-67 | Human | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | # Publications diff --git a/docs/supporting_material/PD-1_BV421/0000-0003-1118-7432.md b/docs/supporting_material/PD-1_BV421/0000-0003-1118-7432.md new file mode 100644 index 0000000..9585f8d --- /dev/null +++ b/docs/supporting_material/PD-1_BV421/0000-0003-1118-7432.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| Q02242 | Primary Antibody | PD-1 | Mouse | Rat | IgG2a | 29F.1.A12 | BioLegend | 135218 | BV421 | AB_2561447 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + + +| Mouse lymph node: PD-1 (cyan, catalog number 135218) and CD11c (magenta, catalog number 117312) | +|:-------:| +| ![](Mouse_LN_PD1_cyan_135218_CD11c_magenta_117312.jpg) | diff --git a/docs/supporting_material/PD-1_BV421/0000-0003-4379-8967.md b/docs/supporting_material/PD-1_BV421/0000-0003-4379-8967.md index 1aac614..3f5e6f5 100644 --- a/docs/supporting_material/PD-1_BV421/0000-0003-4379-8967.md +++ b/docs/supporting_material/PD-1_BV421/0000-0003-4379-8967.md @@ -6,7 +6,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:------------|:---------------|:-----------------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:------------| -| Q02242 | Primary Antibody | PD-1 | Mouse | Rat | IgG2a | 29F.1.A12 | BioLegend | 135218 | BV421 | AB_2561447 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| Q02242 | Primary Antibody | PD-1 | Mouse | Rat | IgG2a | 29F.1.A12 | BioLegend | 135218 | BV421 | AB_2561447 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications)]; [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | | Q15116 | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | BioLegend | 329920 | BV421 | AB_10960742 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | # Publications diff --git a/docs/supporting_material/PD-1_BV421/Mouse_LN_PD1_cyan_135218_CD11c_magenta_117312.jpg b/docs/supporting_material/PD-1_BV421/Mouse_LN_PD1_cyan_135218_CD11c_magenta_117312.jpg new file mode 100644 index 0000000..09a4180 Binary files /dev/null and b/docs/supporting_material/PD-1_BV421/Mouse_LN_PD1_cyan_135218_CD11c_magenta_117312.jpg differ diff --git a/docs/supporting_material/Rabbit_IgG__H+L__AF532/0000-0003-1118-7432.md b/docs/supporting_material/Rabbit_IgG__H+L__AF532/0000-0003-1118-7432.md new file mode 100644 index 0000000..8cff50b --- /dev/null +++ b/docs/supporting_material/Rabbit_IgG__H+L__AF532/0000-0003-1118-7432.md @@ -0,0 +1,21 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-------------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11009 | AF532 | AB_2534076 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | NA | [0000-0003-1118-7432](https://orcid.org/0000-0003-1118-7432) | | + +# Publications + + + +# Additional Notes + + + +| Mouse lymph node: CD35 (yellow, catalog number 740132) and Collagen IV (blue, catalog number ab19808 and A-11009) | +|:-------:| +| ![](../CD35_BV510/Mouse_LN_CD35_yellow_BD_740132_ColIV_blue_ab19808_A-11009.jpg) |