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diff --git a/docs/reagent_resources.md b/docs/reagent_resources.md index 9579ca9..f4aa047 100644 --- a/docs/reagent_resources.md +++ b/docs/reagent_resources.md @@ -15,76 +15,78 @@ Additional information on fluorescent probes tested by the IBEX Imaging Communit | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | |:-------------------------------------------------------------------------------------------------------|:----------------------------------------------------------------|:--------------------------------------------------|:-------------------------------------|:-----------------|:------------|:-------------------|:-------------------------------------------------------------------------------------------------|:--------------------------|:----------------------|:------------|:---------------|:-----------------------|:----------------------------------------|:---------------------------------|:--------------------------|:-----------------------|:-------------------------------------------------------------------------------------------|:-----------------------------------------------------------------------|:------------|:-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|:----------------------------------------------------------------------------|:--------------------| | [Q9BYF1](https://www.uniprot.org/uniprotkb/Q9BYF1) | Primary Antibody | ACE2 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF933 (Unconjugated) | AF555 | AB_2892761 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/ACE2_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9GZX7](https://www.uniprot.org/uniprotkb/Q9GZX7) | Primary Antibody | AID | Human | Mouse | IgG1 | 39-2500 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | ZA001 | Unconjugated | AB_2533403 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/AID_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9GZX7](https://www.uniprot.org/uniprotkb/Q9GZX7) | Primary Antibody | AID | Human | Rat | IgG2a | mAID-2 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 14-5959-82 | Unconjugated | AB_10669583 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/AID_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9GZX7](https://www.uniprot.org/uniprotkb/Q9GZX7) | Primary Antibody | AID | Human | Mouse | IgG1 | 39-2500 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | ZA001 | Unconjugated | AB_2533403 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/AID_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4/asm-1 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-34522IR | DL755 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_DL755/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4/asm-1 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-34522IR | DL755 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_DL755/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-9760-82 | eF570 | AB_2573631 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-9760-82 | eF570 | AB_2573631 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-9760-82 | eF570 | AB_2573631 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-3697-5726](supporting_material/Alpha_smooth_muscle_actin_eF570/0000-0003-3697-5726.md) | NA | 0000-0003-3697-5726 | | [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-3697-5726](supporting_material/Alpha_smooth_muscle_actin_eF660/0000-0003-3697-5726.md) | NA | 0000-0003-3697-5726 | +| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | | [P62737](https://www.uniprot.org/uniprotkb/P62737) | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P62737](https://www.uniprot.org/uniprotkb/P62737) | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P62737](https://www.uniprot.org/uniprotkb/P62737) | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | [Abcam](https://www.abcam.com/) | ab184675 | AF488 | AB_2832195 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | +| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Novus Biologicals](https://www.novusbio.com/) | IC1420S-025 | AF750 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | No | [0000-0002-5187-810X](supporting_material/Alpha_smooth_muscle_actin_AF750/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | | [P62737](https://www.uniprot.org/uniprotkb/P62737) | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-9760-82 | eF570 | AB_2573631 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-3697-5726](supporting_material/Alpha_smooth_muscle_actin_eF570/0000-0003-3697-5726.md) | NA | 0000-0003-3697-5726 | +| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_eF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_eF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_eF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P62737](https://www.uniprot.org/uniprotkb/P62737) | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-9760-82 | eF570 | AB_2573631 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-9760-82 | eF570 | AB_2573631 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4/asm-1 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-34522IR | DL755 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_DL755/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4/asm-1 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-34522IR | DL755 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_DL755/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P62737](https://www.uniprot.org/uniprotkb/P62737) | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-1461-0999](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | +| NA | Primary Antibody | Alpha smooth muscle actin | Canine | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0001-5088-7808](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | +| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P62737](https://www.uniprot.org/uniprotkb/P62737) | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | NA | Primary Antibody | Alpha smooth muscle actin | Canine | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-9760-82 | eF570 | AB_2573631 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0001-5088-7808](supporting_material/Alpha_smooth_muscle_actin_eF570/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | -| NA | Primary Antibody | Alpha smooth muscle actin | Canine | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0001-5088-7808](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | +| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-9760-82 | eF570 | AB_2573631 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P62737](https://www.uniprot.org/uniprotkb/P62737) | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-1461-0999](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | | [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | +| NA | Primary Antibody | Alpha smooth muscle actin | Rhesus Macaque | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | [0009-0004-2746-8743](supporting_material/Alpha_smooth_muscle_actin_AF488/0009-0004-2746-8743.md) | NA | 0009-0004-2746-8743 | +| [P62737](https://www.uniprot.org/uniprotkb/P62737) | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_eF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_eF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P62737](https://www.uniprot.org/uniprotkb/P62737) | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0001-5813-2581](supporting_material/Alpha_smooth_muscle_actin_eF660/0000-0001-5813-2581.md) | NA | 0000-0001-5813-2581 | | [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-9760-82 | eF660 | AB_2574362 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_eF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Novus Biologicals](https://www.novusbio.com/) | IC1420S-025 | AF750 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | No | [0000-0002-5187-810X](supporting_material/Alpha_smooth_muscle_actin_AF750/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | -| [P62737](https://www.uniprot.org/uniprotkb/P62737) | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-9760-82 | eF570 | AB_2573631 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | -| NA | Primary Antibody | Alpha smooth muscle actin | Rhesus Macaque | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | [0009-0004-2746-8743](supporting_material/Alpha_smooth_muscle_actin_AF488/0009-0004-2746-8743.md) | NA | 0009-0004-2746-8743 | -| [P62737](https://www.uniprot.org/uniprotkb/P62737) | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | [Abcam](https://www.abcam.com/) | ab184675 | AF488 | AB_2832195 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | -| [P62736](https://www.uniprot.org/uniprotkb/P62736) | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P62737](https://www.uniprot.org/uniprotkb/P62737) | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P25054](https://www.uniprot.org/uniprotkb/P25054) | Secondary Antibody | APC | Human | Mouse | IgG2b | 936809 | [R&D Systems](https://www.rndsystems.com/) | FAB8927R-100UG | AF647 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/APC_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P29972](https://www.uniprot.org/uniprotkb/P29972) | Primary Antibody | Aquaporin 1 | Human | Rabbit | IgG | EPR11588(B) | [Abcam](https://www.abcam.com/) | ab225225 | AF647 | AB_2810992 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-2950-2683](supporting_material/Aquaporin_1_AF647/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | | [P41181](https://www.uniprot.org/uniprotkb/P41181) | Primary Antibody | Aquaporin 2 | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NBP2-33472 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/Aquaporin_2_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P41181](https://www.uniprot.org/uniprotkb/P41181) | Primary Antibody | Aquaporin 2 | Human | Goat | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NBP1-70378 | Unconjugated | AB_11011225 | Stock | Multiplexed 2D Imaging | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/Aquaporin_2_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P05089](https://www.uniprot.org/uniprotkb/P05089) | Primary Antibody | Arginase-1 | Mouse | Rabbit | IgG | D4E3M | [Cell Signaling Technology](https://www.cellsignal.com/) | 93668 | Unconjugated | AB_2800207 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0001-5813-2581](supporting_material/Arginase-1_Unconjugated/0000-0001-5813-2581.md) | NA | 0000-0001-5813-2581 | | [P36575](https://www.uniprot.org/uniprotkb/P36575) | Primary Antibody | Arrestin C | Human | Mouse | IgG1 | 7G6 | [MilliporeSigma](https://www.sigmaaldrich.com/) | MABN2636-100UG | Unconjugated | AB_2935804 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | [0000-0003-2088-8310](supporting_material/Arrestin_C_Unconjugated/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [P00966](https://www.uniprot.org/uniprotkb/P00966) | Primary Antibody | ASS1 | Human | Rabbit | IgG | EPR12398 | [Abcam](https://www.abcam.com/) | ab170952 | Unconjugated | AB_2892766 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-1431-1398](supporting_material/ASS1_Unconjugated/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | -| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BD Biosciences](https://www.bdbiosciences.com/) | 557683 | AF647 | AB_396793 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/B220_AF647/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | +| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BioLegend](https://www.biolegend.com/) | 103226 | AF647 | AB_389330 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/B220_AF647/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | | NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 42-0452-82 | eF615 | AB_10852702 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/B220_eF615/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-0452-80 | eF570 | AB_2573598 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/B220_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BioLegend](https://www.biolegend.com/) | 103232 | AF700 | AB_493717 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/B220_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BioLegend](https://www.biolegend.com/) | 103226 | AF647 | AB_389330 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-1461-0999](supporting_material/B220_AF647/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | -| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BD Biosciences](https://www.bdbiosciences.com/) | 553090 | PE | AB_394619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/B220_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BioLegend](https://www.biolegend.com/) | 103251 | BV421 | AB_2562905 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/B220_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BioLegend](https://www.biolegend.com/) | 103248 | BV510 | AB_2650679 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/B220_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BD Biosciences](https://www.bdbiosciences.com/) | 557669 | AF488 | AB_396781 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/B220_AF488/0000-0003-4379-8967.md), [0000-0003-1118-7432](supporting_material/B220_AF488/0000-0003-1118-7432.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BD Biosciences](https://www.bdbiosciences.com/) | 553090 | PE | AB_394619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/B220_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BD Biosciences](https://www.bdbiosciences.com/) | 553090 | PE | AB_394619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/B220_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BioLegend](https://www.biolegend.com/) | 103226 | AF647 | AB_389330 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/B220_AF647/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | | NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0452-82 | AF532 | AB_11218492 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/B220_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P10415](https://www.uniprot.org/uniprotkb/P10415) | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | [BioLegend](https://www.biolegend.com/) | 658705 | AF647 | AB_2563279 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/BCL2_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P10415](https://www.uniprot.org/uniprotkb/P10415) | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | [Abcam](https://www.abcam.com/) | ab236221 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/BCL2_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P10415](https://www.uniprot.org/uniprotkb/P10415) | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | [BioLegend](https://www.biolegend.com/) | 658703 | AF488 | AB_2563151 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/BCL2_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P10415](https://www.uniprot.org/uniprotkb/P10415) | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | [BioLegend](https://www.biolegend.com/) | 658707 | PE | AB_2563281 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/BCL2_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BioLegend](https://www.biolegend.com/) | 103251 | BV421 | AB_2562905 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/B220_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BioLegend](https://www.biolegend.com/) | 103232 | AF700 | AB_493717 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/B220_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BD Biosciences](https://www.bdbiosciences.com/) | 557669 | AF488 | AB_396781 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/B220_AF488/0000-0003-4379-8967.md), [0000-0003-1118-7432](supporting_material/B220_AF488/0000-0003-1118-7432.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BD Biosciences](https://www.bdbiosciences.com/) | 553090 | PE | AB_394619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/B220_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BioLegend](https://www.biolegend.com/) | 103248 | BV510 | AB_2650679 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/B220_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BioLegend](https://www.biolegend.com/) | 103226 | AF647 | AB_389330 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-1461-0999](supporting_material/B220_AF647/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | +| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-0452-80 | eF570 | AB_2573598 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/B220_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | [BD Biosciences](https://www.bdbiosciences.com/) | 557683 | AF647 | AB_396793 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/B220_AF647/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | | [P10415](https://www.uniprot.org/uniprotkb/P10415) | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | [Abcam](https://www.abcam.com/) | ab236221 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/BCL2_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P10415](https://www.uniprot.org/uniprotkb/P10415) | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | [BioLegend](https://www.biolegend.com/) | 658707 | PE | AB_2563281 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/BCL2_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P10415](https://www.uniprot.org/uniprotkb/P10415) | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | [BioLegend](https://www.biolegend.com/) | 658707 | PE | AB_2563281 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/BCL2_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P10415](https://www.uniprot.org/uniprotkb/P10415) | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | [Abcam](https://www.abcam.com/) | ab236221 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/BCL2_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P10415](https://www.uniprot.org/uniprotkb/P10415) | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | [BioLegend](https://www.biolegend.com/) | 658703 | AF488 | AB_2563151 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/BCL2_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P10415](https://www.uniprot.org/uniprotkb/P10415) | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | [Abcam](https://www.abcam.com/) | ab236221 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/BCL2_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P10415](https://www.uniprot.org/uniprotkb/P10415) | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | [BioLegend](https://www.biolegend.com/) | 658705 | AF647 | AB_2563279 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/BCL2_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P10415](https://www.uniprot.org/uniprotkb/P10415) | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | [Abcam](https://www.abcam.com/) | ab236221 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/BCL2_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P41182](https://www.uniprot.org/uniprotkb/P41182) | Primary Antibody | BCL6 | Human | Mouse | IgG1 | K112-91 | [BD Biosciences](https://www.bdbiosciences.com/) | 561525 | AF647 | AB_10898007 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/BCL6_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P41182](https://www.uniprot.org/uniprotkb/P41182) | Primary Antibody | BCL6 | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NBP2-59786 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/BCL6_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P41183](https://www.uniprot.org/uniprotkb/P41183) | Primary Antibody | BCL6 | Mouse | Mouse | IgG1 | K112-91 | [BD Biosciences](https://www.bdbiosciences.com/) | 561525 | AF647 | AB_10898007 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/BCL6_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P41182](https://www.uniprot.org/uniprotkb/P41182) | Primary Antibody | BCL6 | Human | Mouse | IgG1 | K112-91 | [BD Biosciences](https://www.bdbiosciences.com/) | 561525 | AF647 | AB_10898007 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/BCL6_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P41182](https://www.uniprot.org/uniprotkb/P41182) | Primary Antibody | BCL6 | Human | Mouse | IgG1 | K112-91 | [BD Biosciences](https://www.bdbiosciences.com/) | 561525 | AF647 | AB_10898007 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/BCL6_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P41182](https://www.uniprot.org/uniprotkb/P41182) | Primary Antibody | BCL6 | Human | Mouse | IgG1 | GI191E | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 14-9887-82 | Unconjugated | AB_10669166 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/BCL6_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P41183](https://www.uniprot.org/uniprotkb/P41183) | Primary Antibody | BCL6 | Mouse | Mouse | IgG1 | K112-91 | [BD Biosciences](https://www.bdbiosciences.com/) | 561525 | AF647 | AB_10898007 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/BCL6_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P41182](https://www.uniprot.org/uniprotkb/P41182) | Primary Antibody | BCL6 | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NBP2-59786 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/BCL6_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9W6A8](https://www.uniprot.org/uniprotkb/Q9W6A8) | Primary Antibody | Blue opsin | Zebrafish | Rabbit | IgG | Polyclonal | [Kerafast](https://www.kerafast.com/) | EJH012 | Unconjugated | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Blue_opsin_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [P35226](https://www.uniprot.org/uniprotkb/P35226) | Primary Antibody | BMI-1 | Human | Mouse | IgG2b | LLBmi1-1 | [Novus Biologicals](https://www.novusbio.com/) | NBP1-96140AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/BMI-1_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01106](https://www.uniprot.org/uniprotkb/P01106) | Primary Antibody | c-Myc | Human | Mouse | IgG1 | 9E10 | [Novus Biologicals](https://www.novusbio.com/) | NB600-302IR | DL755 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/c-Myc_DL755/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01106](https://www.uniprot.org/uniprotkb/P01106) | Primary Antibody | c-Myc | Human | Mouse | IgG1 | 9E10 | [R&D Systems](https://www.rndsystems.com/) | MAB3696-SP | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/c-Myc_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P05937](https://www.uniprot.org/uniprotkb/P05937) | Primary Antibody | Calbindin | Human | Rabbit | IgG | Polyclonal | [MilliporeSigma](https://www.sigmaaldrich.com/) | ABN2192 | Unconjugated | AB_2935805 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | [0000-0003-2088-8310](supporting_material/Calbindin_Unconjugated/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [E9QJF8](https://www.uniprot.org/uniprotkb/E9QJF8) | Primary Antibody | Calbindin | Zebrafish | Rabbit | IgG | Polyclonal | [Swant](https://webshop.swant.com/) | CB38a | Unconjugated | AB_10000340 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Calbindin_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | +| [P22676](https://www.uniprot.org/uniprotkb/P22676) | Primary Antibody | Calretinin | Mouse | Mouse | IgG1 | 6B8.2 | [MilliporeSigma](https://www.sigmaaldrich.com/) | MAB1568 | Unconjugated | AB_94259 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | [0000-0003-2088-8310](supporting_material/Calretinin_Unconjugated/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [Q6PC44](https://www.uniprot.org/uniprotkb/Q6PC44) | Primary Antibody | Calretinin | Zebrafish | Rabbit | IgG | Polyclonal | [Swant](https://webshop.swant.com/) | 7697 | Unconjugated | AB_2721226 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Calretinin_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [A0A1A8U8H3](https://www.uniprot.org/uniprotkb/A0A1A8U8H3) | Primary Antibody | Calretinin | Turquoise Killifish | Rabbit | IgG | Polyclonal | [Swant](https://webshop.swant.com/) | 7697 | Unconjugated | AB_2721226 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Calretinin_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| [P22676](https://www.uniprot.org/uniprotkb/P22676) | Primary Antibody | Calretinin | Mouse | Mouse | IgG1 | 6B8.2 | [MilliporeSigma](https://www.sigmaaldrich.com/) | MAB1568 | Unconjugated | AB_94259 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | [0000-0003-2088-8310](supporting_material/Calretinin_Unconjugated/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | NA | Primary Antibody | Carbonic anhydrase 1 | Zebrafish | Rabbit | IgG | EPR5193 | [Abcam](https://www.abcam.com/) | ab108367 | Unconjugated | AB_10863424 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Carbonic_anhydrase_1_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [P07711](https://www.uniprot.org/uniprotkb/P07711) | Primary Antibody | Cathepsin L | Human | Rat | IgG2a | 204101 | [R&D Systems](https://www.rndsystems.com/) | MAB-9521 | Unconjugated | AB_2087829 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Cathepsin_L_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q99731](https://www.uniprot.org/uniprotkb/Q99731) | Primary Antibody | CCL19 | Human | Mouse | IgG1 | OTI2A12 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-71278AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-3882-457X](supporting_material/CCL19_AF532/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | @@ -92,481 +94,485 @@ Additional information on fluorescent probes tested by the IBEX Imaging Communit | [P32248](https://www.uniprot.org/uniprotkb/P32248) | Primary Antibody | CCR7 | Human | Rabbit | IgG | Y59 | [Abcam](https://www.abcam.com/) | ab32527 | Unconjugated | AB_726208 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CCR7_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P32248](https://www.uniprot.org/uniprotkb/P32248) | Primary Antibody | CCR7 | Human | Goat | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NB100-712SS | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-3882-457X](supporting_material/CCR7_Unconjugated/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | | [P32248](https://www.uniprot.org/uniprotkb/P32248) | Primary Antibody | CCR7 | Human | Rabbit | IgG | Polyclonal | [LSBio](https://www.lsbio.com/) | LS-C210538 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CCR7_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P51686](https://www.uniprot.org/uniprotkb/P51686) | Primary Antibody | CCR9 | Human | Mouse | IgG2a | 112509 | [Novus Biologicals](https://www.novusbio.com/) | FAB179G-100UG | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-3882-457X](supporting_material/CCR9_AF488/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | | [P51686](https://www.uniprot.org/uniprotkb/P51686) | Primary Antibody | CCR9 | Human | Rabbit | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | PA5-33407 | Unconjugated | AB_2550787 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-3882-457X](supporting_material/CCR9_Unconjugated/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | +| [P51686](https://www.uniprot.org/uniprotkb/P51686) | Primary Antibody | CCR9 | Human | Mouse | IgG2a | 112509 | [Novus Biologicals](https://www.novusbio.com/) | FAB179G-100UG | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-3882-457X](supporting_material/CCR9_AF488/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | +| [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF1182 | Unconjugated | AB_354652 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD10_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | [Caprico Biotechnologies](https://www.capricobio.com/) | 103901 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD10_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Rat | IgG2b | 200103 | [R&D Systems](https://www.rndsystems.com/) | MAB1126-SP | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD10_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Mouse | IgG1 | HI10a | [BioLegend](https://www.biolegend.com/) | 312204 | PE | AB_314915 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD10_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | [Caprico Biotechnologies](https://www.capricobio.com/) | 103924 | PE | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD10_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Mouse | IgG1 | HI10a | [BioLegend](https://www.biolegend.com/) | 312204 | PE | AB_314915 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD10_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | [Caprico Biotechnologies](https://www.capricobio.com/) | 103924 | PE | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD10_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF1182 | Unconjugated | AB_354652 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD10_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF1182 | Unconjugated | AB_354652 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD10_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Rat | IgG2b | 200103 | [R&D Systems](https://www.rndsystems.com/) | MAB1126-SP | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD10_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1039134 | iF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD10_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Mouse | IgG1 | HI10a | [BioLegend](https://www.biolegend.com/) | 312204 | PE | AB_314915 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD10_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Mouse | IgG1 | HI10a | [BioLegend](https://www.biolegend.com/) | 312204 | PE | AB_314915 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD10_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | [Caprico Biotechnologies](https://www.capricobio.com/) | 103924 | PE | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD10_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF1182 | Unconjugated | AB_354652 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD10_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF1182 | Unconjugated | AB_354652 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD10_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08473](https://www.uniprot.org/uniprotkb/P08473) | Primary Antibody | CD10 | Human | Mouse | IgG1 | HI10a | [BioLegend](https://www.biolegend.com/) | 312204 | PE | AB_314915 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD10_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P38570](https://www.uniprot.org/uniprotkb/P38570) | Primary Antibody | CD103 | Human | Rabbit | IgG | ITGAE/3904R | [Novus Biologicals](https://www.novusbio.com/) | NBP3-07530-100ug | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD103_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P38570](https://www.uniprot.org/uniprotkb/P38570) | Primary Antibody | CD103 | Human | Rabbit | IgG | ITGAE/3904R | [Novus Biologicals](https://www.novusbio.com/) | NBP3-08855AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD103_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P17813](https://www.uniprot.org/uniprotkb/P17813) | Primary Antibody | CD105 (Endoglin) | Human | Mouse | IgG1 | SN6h | [BioLegend](https://www.biolegend.com/) | 800505 | FITC | AB_2687058 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0002-3882-457X](supporting_material/CD105__Endoglin__FITC/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | -| [P19320](https://www.uniprot.org/uniprotkb/P19320) | Primary Antibody | CD106 | Human | Mouse | IgG1 | STA | [BioLegend](https://www.biolegend.com/) | 305806 | PE | AB_314561 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD106_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P19320](https://www.uniprot.org/uniprotkb/P19320) | Primary Antibody | CD106 | Human | Mouse | IgG1 | STA | [BioLegend](https://www.biolegend.com/) | 305806 | PE | AB_314561 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD106_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P29533](https://www.uniprot.org/uniprotkb/P29533) | Primary Antibody | CD106 | Mouse | Rat | IgG2a | 429 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-1061-82 | eF660 | AB_11219864 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD106_eF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P29533](https://www.uniprot.org/uniprotkb/P29533) | Primary Antibody | CD106 | Mouse | Rat | IgG2a | 429 | [BioLegend](https://www.biolegend.com/) | 105710 | AF488 | AB_493427 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD106_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P19320](https://www.uniprot.org/uniprotkb/P19320) | Primary Antibody | CD106 | Human | Mouse | IgG1 | STA | [BioLegend](https://www.biolegend.com/) | 305806 | PE | AB_314561 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD106_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P19320](https://www.uniprot.org/uniprotkb/P19320) | Primary Antibody | CD106 | Human | Mouse | IgG1 | STA | [BioLegend](https://www.biolegend.com/) | 305806 | PE | AB_314561 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD106_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P05532](https://www.uniprot.org/uniprotkb/P05532) | Primary Antibody | CD117 | Mouse | Rat | IgG2b | 2B8 | [BioLegend](https://www.biolegend.com/) | 105827 | BV421 | AB_10898120 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD117_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [O97799](https://www.uniprot.org/uniprotkb/O97799) | Primary Antibody | CD117 | Canine | Rabbit | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | BS-0672R | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/CD117_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | -| [P10721](https://www.uniprot.org/uniprotkb/P10721) | Primary Antibody | CD117 | Human | Mouse | IgG1 | 104D2 | [BioLegend](https://www.biolegend.com/) | 313234 | AF488 | AB_2566221 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD117_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P10721](https://www.uniprot.org/uniprotkb/P10721) | Primary Antibody | CD117 | Human | Mouse | IgG1 | 104D2 | [BioLegend](https://www.biolegend.com/) | 313203 | PE | AB_314983 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0002-5187-810X](supporting_material/CD117_PE/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | -| [P05532](https://www.uniprot.org/uniprotkb/P05532) | Primary Antibody | CD117 | Mouse | Rat | IgG2b | 2B8 | [BioLegend](https://www.biolegend.com/) | 105827 | BV421 | AB_10898120 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD117_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P10721](https://www.uniprot.org/uniprotkb/P10721) | Primary Antibody | CD117 | Human | Mouse | IgG1 | 104D2 | [BioLegend](https://www.biolegend.com/) | 313234 | AF488 | AB_2566221 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD117_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9UQ88](https://www.uniprot.org/uniprotkb/Q9UQ88) | Primary Antibody | CD11a | Human | Mouse | IgG1 | HI111 | [AAT Bioquest](https://www.aatbio.com/) | 101100C0 | iF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD11a_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | [BD Biosciences](https://www.bdbiosciences.com/) | 101217 | PE | AB_396680 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-8909-5039](supporting_material/CD11b_PE/0000-0002-8909-5039.md) | NA | 0000-0002-8909-5039 | +| [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | [Bio-Rad](https://www.bio-rad.com/) | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11b_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | [Bio-Rad](https://www.bio-rad.com/) | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11b_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | [Bio-Rad](https://www.bio-rad.com/) | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11b_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P11215](https://www.uniprot.org/uniprotkb/P11215) | Primary Antibody | CD11b | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NB110-89474AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD11b_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P11215](https://www.uniprot.org/uniprotkb/P11215) | Primary Antibody | CD11b | Human | Rabbit | IgG | EPR1344 | [Abcam](https://www.abcam.com/) | ab133357 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD11b_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | [Bio-Rad](https://www.bio-rad.com/) | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11b_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | [BD Biosciences](https://www.bdbiosciences.com/) | 557397 | PE | AB_396680 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0001-5813-2581](supporting_material/CD11b_PE/0000-0001-5813-2581.md) | NA | 0000-0001-5813-2581 | +| [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | [Bio-Rad](https://www.bio-rad.com/) | MCA711PE | PE | AB_323678 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11b_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | [BioLegend](https://www.biolegend.com/) | 101217 | AF488 | AB_389305 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/CD11b_AF488/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | +| [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | [BioLegend](https://www.biolegend.com/) | 101208 | PE | AB_312791 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/CD11b_PE/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | +| [P11215](https://www.uniprot.org/uniprotkb/P11215) | Primary Antibody | CD11b | Human | Rabbit | IgG | SP330 | [Abcam](https://www.abcam.com/) | ab241408 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD11b_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P11215](https://www.uniprot.org/uniprotkb/P11215) | Primary Antibody | CD11b | Human | Rabbit | IgG | EP1345Y | [Abcam](https://www.abcam.com/) | ab187537 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD11b_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P11215](https://www.uniprot.org/uniprotkb/P11215) | Primary Antibody | CD11b | Human | Rabbit | IgG | EPR1344 | [Abcam](https://www.abcam.com/) | ab133357 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD11b_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P11215](https://www.uniprot.org/uniprotkb/P11215) | Primary Antibody | CD11b | Human | Rabbit | IgG | EP1345Y | [Abcam](https://www.abcam.com/) | ab187537 (Unconjugated) | AF647 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD11b_AF647/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P11215](https://www.uniprot.org/uniprotkb/P11215) | Primary Antibody | CD11b | Human | Rabbit | IgG | SP330 | [Abcam](https://www.abcam.com/) | ab241408 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD11b_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | [BioLegend](https://www.biolegend.com/) | 101208 | PE | AB_312791 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/CD11b_PE/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | -| NA | Primary Antibody | CD11b | Canine | Mouse | IgG1 | CA16.3E10 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA5-16604 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/CD11b_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | -| [P11215](https://www.uniprot.org/uniprotkb/P11215) | Primary Antibody | CD11b | Human | Rabbit | IgG | EP1345Y | [Abcam](https://www.abcam.com/) | ab187537 (Unconjugated) | AF647 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD11b_AF647/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P11215](https://www.uniprot.org/uniprotkb/P11215) | Primary Antibody | CD11b | Human | Rabbit | IgG | SP331 | [Abcam](https://www.abcam.com/) | ab238794 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD11b_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | [Bio-Rad](https://www.bio-rad.com/) | MCA711PE | PE | AB_323678 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11b_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | [Bio-Rad](https://www.bio-rad.com/) | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11b_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | [Bio-Rad](https://www.bio-rad.com/) | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11b_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | [BD Biosciences](https://www.bdbiosciences.com/) | 101217 | PE | AB_396680 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-8909-5039](supporting_material/CD11b_PE/0000-0002-8909-5039.md) | NA | 0000-0002-8909-5039 | -| [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | [Bio-Rad](https://www.bio-rad.com/) | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11b_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P11215](https://www.uniprot.org/uniprotkb/P11215) | Primary Antibody | CD11b | Human | Rabbit | IgG | EP1345Y | [Abcam](https://www.abcam.com/) | ab187537 (Unconjugated) | AF647 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD11b_AF647/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| NA | Primary Antibody | CD11b | Canine | Mouse | IgG1 | CA16.3E10 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA5-16604 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/CD11b_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | +| [P11215](https://www.uniprot.org/uniprotkb/P11215) | Primary Antibody | CD11b | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NB110-89474AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD11b_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | [BioLegend](https://www.biolegend.com/) | 101208 | PE | AB_312791 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/CD11b_PE/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | -| [P24788](https://www.uniprot.org/uniprotkb/P24788) | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | [BioLegend](https://www.biolegend.com/) | 101217 | AF488 | AB_389305 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/CD11b_AF488/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | -| [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | [BD Biosciences](https://www.bdbiosciences.com/) | 561352 | AF700 | AB_10612006 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MCD11c20 | AF488 | AB_10373244 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_AF488/0000-0003-4379-8967.md), [0000-0002-1461-0999](supporting_material/CD11c_AF488/0000-0002-1461-0999.md) | NA | 0000-0003-4379-8967 | +| [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Mouse | IgG1 | 3.9 | [BioLegend](https://www.biolegend.com/) | 301603 | FITC | AB_314173 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD11c_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | [BD Biosciences](https://www.bdbiosciences.com/) | 561352 | AF700 | AB_10612006 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | [BD Biosciences](https://www.bdbiosciences.com/) | 561352 | AF700 | AB_10612006 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Rabbit | IgG | EP1347Y | [Abcam](https://www.abcam.com/) | ab216655 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD11c_AF555/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117308 | PE | AB_313777 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/CD11c_PE/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | +| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MCD11c20 | AF488 | AB_10373244 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_AF488/0000-0003-4379-8967.md), [0000-0002-1461-0999](supporting_material/CD11c_AF488/0000-0002-1461-0999.md) | NA | 0000-0003-4379-8967 | +| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/CD11c_AF647/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | | [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_AF647/0000-0003-4379-8967.md), [0000-0003-1118-7432](supporting_material/CD11c_AF647/0000-0003-1118-7432.md) | NA | 0000-0003-4379-8967 | -| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | [BD Biosciences](https://www.bdbiosciences.com/) | 555392 | PE | AB_395793 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | [BD Biosciences](https://www.bdbiosciences.com/) | 561355 | FITC | AB_10611872 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MCD11c20 | AF488 | AB_10373244 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0001-5813-2581](supporting_material/CD11c_AF488/0000-0001-5813-2581.md) | NA | 0000-0001-5813-2581 | +| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117314 | AF647 | AB_492850 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](supporting_material/CD11c_AF647/0000-0002-6944-6997.md) | NA | 0000-0002-6944-6997 | | [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117371 | Spark Red 718 | AB_2924457 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD11c_Spark_Red_718/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | [BD Biosciences](https://www.bdbiosciences.com/) | 562561 | BV421 | AB_2737656 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD11c_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | [BD Biosciences](https://www.bdbiosciences.com/) | 563026 | BV510 | AB_2737960 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD11c_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | [BD Biosciences](https://www.bdbiosciences.com/) | 561355 | FITC | AB_10611872 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Mouse | IgG1 | 3.9 | [BioLegend](https://www.biolegend.com/) | 301603 | FITC | AB_314173 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD11c_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | [BD Biosciences](https://www.bdbiosciences.com/) | 561352 | AF700 | AB_10612006 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117346 | AF594 | AB_2563323 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD11c_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117308 | PE | AB_313777 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/CD11c_PE/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | +| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Rabbit | IgG | EP1347Y | [Abcam](https://www.abcam.com/) | ab216655 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD11c_AF555/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Rabbit | IgG | EP1347Y | [Abcam](https://www.abcam.com/) | ab216655 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD11c_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117314 | AF647 | AB_492850 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](supporting_material/CD11c_AF647/0000-0002-6944-6997.md) | NA | 0000-0002-6944-6997 | -| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/CD11c_AF647/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | | [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Rabbit | IgG | EP1347Y | [Abcam](https://www.abcam.com/) | ab216655 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD11c_AF555/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [Q9QXH4](https://www.uniprot.org/uniprotkb/Q9QXH4) | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | [BioLegend](https://www.biolegend.com/) | 117346 | AF594 | AB_2563323 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD11c_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | [BD Biosciences](https://www.bdbiosciences.com/) | 555392 | PE | AB_395793 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | [BD Biosciences](https://www.bdbiosciences.com/) | 561352 | AF700 | AB_10612006 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD11c_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | [BioLegend](https://www.biolegend.com/) | 356517 | BV510 | AB_2562661 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD138_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P18828](https://www.uniprot.org/uniprotkb/P18828) | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | [BioLegend](https://www.biolegend.com/) | 142526 | AF647 | AB_2566239 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD138_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P18828](https://www.uniprot.org/uniprotkb/P18828) | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | [BD Biosciences](https://www.bdbiosciences.com/) | 562610 | BV421 | AB_11153126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD138_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P18828](https://www.uniprot.org/uniprotkb/P18828) | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | [BD Biosciences](https://www.bdbiosciences.com/) | 562610 | BV421 | AB_11153126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD138_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P20702](https://www.uniprot.org/uniprotkb/P20702) | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | [BD Biosciences](https://www.bdbiosciences.com/) | 562561 | BV421 | AB_2737656 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD11c_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P18828](https://www.uniprot.org/uniprotkb/P18828) | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | [BD Biosciences](https://www.bdbiosciences.com/) | 562610 | BV421 | AB_11153126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD138_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | [BioLegend](https://www.biolegend.com/) | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD138_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | [BioLegend](https://www.biolegend.com/) | 356524 | AF647 | AB_2564250 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD138_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P18828](https://www.uniprot.org/uniprotkb/P18828) | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | [BD Biosciences](https://www.bdbiosciences.com/) | 562610 | BV421 | AB_11153126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD138_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2780 | Unconjugated | AB_442186 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD138_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | [AAT Bioquest](https://www.aatbio.com/) | 113800C0 | iF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD138_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | [BioLegend](https://www.biolegend.com/) | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD138_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | [BioLegend](https://www.biolegend.com/) | 356504 | PE | AB_2561878 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD138_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | [BioLegend](https://www.biolegend.com/) | 356517 | BV510 | AB_2562661 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD138_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P18828](https://www.uniprot.org/uniprotkb/P18828) | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | [BD Biosciences](https://www.bdbiosciences.com/) | 562610 | BV421 | AB_11153126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD138_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | [BioLegend](https://www.biolegend.com/) | 356524 | AF647 | AB_2564250 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD138_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | [BioLegend](https://www.biolegend.com/) | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD138_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | [BioLegend](https://www.biolegend.com/) | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD138_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2780 | Unconjugated | AB_442186 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD138_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | [BioLegend](https://www.biolegend.com/) | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD138_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | [BioLegend](https://www.biolegend.com/) | 356524 | AF647 | AB_2564250 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD138_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P18828](https://www.uniprot.org/uniprotkb/P18828) | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | [BioLegend](https://www.biolegend.com/) | 142526 | AF647 | AB_2566239 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD138_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | [BioLegend](https://www.biolegend.com/) | 356504 | PE | AB_2561878 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD138_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | [BioLegend](https://www.biolegend.com/) | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD138_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | [BioLegend](https://www.biolegend.com/) | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD138_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08571](https://www.uniprot.org/uniprotkb/P08571) | Primary Antibody | CD14 | Human | Mouse | IgG1 | 61D3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0149-41 | AF532 | AB_11218093 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD14_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08571](https://www.uniprot.org/uniprotkb/P08571) | Primary Antibody | CD14 | Human | Rabbit | IgG | EPR3653 | [Abcam](https://www.abcam.com/) | ab226121 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD14_AF555/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P08571](https://www.uniprot.org/uniprotkb/P08571) | Primary Antibody | CD14 | Human | Rabbit | IgG | EPR3653 | [Abcam](https://www.abcam.com/) | ab226121 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD14_AF555/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P08571](https://www.uniprot.org/uniprotkb/P08571) | Primary Antibody | CD14 | Human | Mouse | IgG1 | 61D3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0149-41 | AF532 | AB_11218093 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD14_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P33151](https://www.uniprot.org/uniprotkb/P33151) | Primary Antibody | CD144 (VE-Cadherin) | Human | Mouse | IgG2a | BV9 | [BioLegend](https://www.biolegend.com/) | 348513 | AF647 | AB_2563669 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD144__VE-Cadherin__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P22083](https://www.uniprot.org/uniprotkb/P22083) | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | [BioLegend](https://www.biolegend.com/) | 394702 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD15_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P22083](https://www.uniprot.org/uniprotkb/P22083) | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | [BioLegend](https://www.biolegend.com/) | 394702 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1% PFA Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-2950-2683](supporting_material/CD15_AF532/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | -| [P22083](https://www.uniprot.org/uniprotkb/P22083) | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | [BD Biosciences](https://www.bdbiosciences.com/) | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD15_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P22083](https://www.uniprot.org/uniprotkb/P22083) | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | [BD Biosciences](https://www.bdbiosciences.com/) | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0002-2692-1225](supporting_material/CD15_Unconjugated/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | -| [P22083](https://www.uniprot.org/uniprotkb/P22083) | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | [BD Biosciences](https://www.bdbiosciences.com/) | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD15_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P22083](https://www.uniprot.org/uniprotkb/P22083) | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | [BioLegend](https://www.biolegend.com/) | 394702 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD15_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P22083](https://www.uniprot.org/uniprotkb/P22083) | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | [BD Biosciences](https://www.bdbiosciences.com/) | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD15_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P22083](https://www.uniprot.org/uniprotkb/P22083) | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | [BD Biosciences](https://www.bdbiosciences.com/) | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD15_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P22083](https://www.uniprot.org/uniprotkb/P22083) | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | [BD Biosciences](https://www.bdbiosciences.com/) | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD15_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P22083](https://www.uniprot.org/uniprotkb/P22083) | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | [BioLegend](https://www.biolegend.com/) | 394702 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD15_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P22083](https://www.uniprot.org/uniprotkb/P22083) | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | [BioLegend](https://www.biolegend.com/) | 394702 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1% PFA Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-2950-2683](supporting_material/CD15_AF532/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | | [P22083](https://www.uniprot.org/uniprotkb/P22083) | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | [BD Biosciences](https://www.bdbiosciences.com/) | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD15_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P22083](https://www.uniprot.org/uniprotkb/P22083) | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | [BioLegend](https://www.biolegend.com/) | 394702 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD15_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [A0A0B4J1G0](https://www.uniprot.org/uniprotkb/A0A0B4J1G0) | Primary Antibody | CD16.2 | Mouse | Armenian Hamster | IgG | 9E9 | [BioLegend](https://www.biolegend.com/) | 149503 | PE | AB_2565810 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/CD16.2_PE/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | | [P08101](https://www.uniprot.org/uniprotkb/P08101) | Primary Antibody | CD16/32 | Mouse | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF1460-SP | Unconjugated | NA | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/CD16_32_Unconjugated/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | -| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | 10D6 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA5-11458 | Unconjugated | AB_10982556 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD163_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | EDHu-1 | [Novus Biologicals](https://www.novusbio.com/) | NB110-40686AF647 | AF647 | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/CD163_AF647/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | -| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | [BioLegend](https://www.biolegend.com/) | 333620 | AF647 | AB_2563475 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD163_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | 10D6 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA5-11458 | Unconjugated | AB_10982556 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD163_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 12-1639-41 | PE | AB_1963571 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD163_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | [BioLegend](https://www.biolegend.com/) | 333620 | AF647 | AB_2563475 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD163_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [F7DAU3](https://www.uniprot.org/uniprotkb/F7DAU3) | Primary Antibody | CD163 | Rhesus Macaque | Mouse | IgG2b | EDHu-1 | [Novus Biologicals](https://www.novusbio.com/) | NB110-40686AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD163_AF647/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | 10D6 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA5-11458 | Unconjugated | AB_10982556 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD163_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | [BioLegend](https://www.biolegend.com/) | 333602 (Unconjugated) | AF532 | AB_2892759 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD163_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | [BioLegend](https://www.biolegend.com/) | 333628 | BV510 | AB_2650632 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD163_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | 10D6 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA5-11458 | Unconjugated | AB_10982556 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD163_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | [BioLegend](https://www.biolegend.com/) | 333620 | AF647 | AB_2563475 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD163_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | [BioLegend](https://www.biolegend.com/) | 333611 | BV421 | AB_2562462 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD163_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | [BioLegend](https://www.biolegend.com/) | 333620 | AF647 | AB_2563475 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD163_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | [BioLegend](https://www.biolegend.com/) | 333628 | BV510 | AB_2650632 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD163_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 12-1639-41 | PE | AB_1963571 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD163_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | 10D6 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA5-11458 | Unconjugated | AB_10982556 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD163_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG2b | 6D3.2F3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-36495AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD163_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG2b | EPR19518 | [Abcam](https://www.abcam.com/) | ab213612 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD163_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | [BioLegend](https://www.biolegend.com/) | 333620 | AF647 | AB_2563475 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD163_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | [BioLegend](https://www.biolegend.com/) | 333602 (Unconjugated) | AF532 | AB_2892759 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD163_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | [BioLegend](https://www.biolegend.com/) | 333620 | AF647 | AB_2563475 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD163_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | 10D6 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA5-11458 | Unconjugated | AB_10982556 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD163_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [Q86VB7](https://www.uniprot.org/uniprotkb/Q86VB7) | Primary Antibody | CD163 | Human | Mouse | IgG1 | EDHu-1 | [Novus Biologicals](https://www.novusbio.com/) | NB110-40686AF647 | AF647 | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/CD163_AF647/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | | [Q13740](https://www.uniprot.org/uniprotkb/Q13740) | Primary Antibody | CD166 | Human | Rabbit | IgG | EPR2759 | [Abcam](https://www.abcam.com/) | ab197543 | AF488 | AB_2889210 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD166_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q13740](https://www.uniprot.org/uniprotkb/Q13740) | Primary Antibody | CD166 | Human | Mouse | IgG1 | 3A6 | [BioLegend](https://www.biolegend.com/) | 343903 | PE | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-3882-457X](supporting_material/CD166_PE/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | -| [Q62230](https://www.uniprot.org/uniprotkb/Q62230) | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | [BioLegend](https://www.biolegend.com/) | 142416 | AF594 | AB_2565620 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD169_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q62230](https://www.uniprot.org/uniprotkb/Q62230) | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | [BioLegend](https://www.biolegend.com/) | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD169_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q62230](https://www.uniprot.org/uniprotkb/Q62230) | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | [BioLegend](https://www.biolegend.com/) | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD169_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q62230](https://www.uniprot.org/uniprotkb/Q62230) | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | [BioLegend](https://www.biolegend.com/) | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD169_PE/0000-0003-4379-8967.md), [0000-0003-1118-7432](supporting_material/CD169_PE/0000-0003-1118-7432.md) | NA | 0000-0003-4379-8967 | +| [Q62230](https://www.uniprot.org/uniprotkb/Q62230) | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | [BioLegend](https://www.biolegend.com/) | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD169_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q62230](https://www.uniprot.org/uniprotkb/Q62230) | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | [BioLegend](https://www.biolegend.com/) | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD169_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q62230](https://www.uniprot.org/uniprotkb/Q62230) | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | [BioLegend](https://www.biolegend.com/) | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD169_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q62230](https://www.uniprot.org/uniprotkb/Q62230) | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | [Bio-Rad](https://www.bio-rad.com/) | MCA884F | FITC | AB_324246 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD169_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P49682](https://www.uniprot.org/uniprotkb/P49682) | Primary Antibody | CD183 (CXCR3) | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab154033 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD183__CXCR3__Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q62230](https://www.uniprot.org/uniprotkb/Q62230) | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | [BioLegend](https://www.biolegend.com/) | 142416 | AF594 | AB_2565620 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD169_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P49682](https://www.uniprot.org/uniprotkb/P49682) | Primary Antibody | CD183 (CXCR3) | Human | Mouse | IgG1 | G025H7 | [BioLegend](https://www.biolegend.com/) | 353711 | AF647 | AB_10962946 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD183__CXCR3__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P49682](https://www.uniprot.org/uniprotkb/P49682) | Primary Antibody | CD183 (CXCR3) | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab154033 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD183__CXCR3__Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P49682](https://www.uniprot.org/uniprotkb/P49682) | Primary Antibody | CD183 (CXCR3) | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NBP2-16044 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD183__CXCR3__Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P32302](https://www.uniprot.org/uniprotkb/P32302) | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | [BD Biosciences](https://www.bdbiosciences.com/) | 552032 | Unconjugated | AB_394324 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD185__CXCR5__Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P32302](https://www.uniprot.org/uniprotkb/P32302) | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | [BD Biosciences](https://www.bdbiosciences.com/) | 558112 | AF488 | AB_397034 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD185__CXCR5__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P32302](https://www.uniprot.org/uniprotkb/P32302) | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | [BD Biosciences](https://www.bdbiosciences.com/) | 552032 | Unconjugated | AB_394324 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD185__CXCR5__Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P32302](https://www.uniprot.org/uniprotkb/P32302) | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | [BD Biosciences](https://www.bdbiosciences.com/) | 552032 | Unconjugated | AB_394324 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD185__CXCR5__Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P32302](https://www.uniprot.org/uniprotkb/P32302) | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | [BD Biosciences](https://www.bdbiosciences.com/) | 552032 | Unconjugated | AB_394324 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD185__CXCR5__Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P15391](https://www.uniprot.org/uniprotkb/P15391) | Primary Antibody | CD19 | Human | Mouse | IgG1 | SJ25C1 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0198-41 | AF532 | AB_11219076 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD19_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P25918](https://www.uniprot.org/uniprotkb/P25918) | Primary Antibody | CD19 | Mouse | Rat | IgG2a | 6D5 | [BioLegend](https://www.biolegend.com/) | 115552 | AF594 | AB_2563459 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/CD19_AF594/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | | [P29017](https://www.uniprot.org/uniprotkb/P29017) | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | [BioLegend](https://www.biolegend.com/) | 331525 | BV421 | AB_10933249 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD1c_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P29017](https://www.uniprot.org/uniprotkb/P29017) | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | [BioLegend](https://www.biolegend.com/) | 331510 | AF647 | AB_1186032 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD1c_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P29017](https://www.uniprot.org/uniprotkb/P29017) | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | [BioLegend](https://www.biolegend.com/) | 331522 | AF488 | AB_10720182 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD1c_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P29017](https://www.uniprot.org/uniprotkb/P29017) | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | [BioLegend](https://www.biolegend.com/) | 331506 | PE | AB_1088999 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD1c_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P29017](https://www.uniprot.org/uniprotkb/P29017) | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | [BioLegend](https://www.biolegend.com/) | 331506 | PE | AB_1088999 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD1c_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P29017](https://www.uniprot.org/uniprotkb/P29017) | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | [BioLegend](https://www.biolegend.com/) | 331506 | PE | AB_1088999 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD1c_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P29017](https://www.uniprot.org/uniprotkb/P29017) | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | [BioLegend](https://www.biolegend.com/) | 331529 | AF700 | AB_2563656 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD1c_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P29017](https://www.uniprot.org/uniprotkb/P29017) | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | [BioLegend](https://www.biolegend.com/) | 331510 | AF647 | AB_1186032 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD1c_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P29017](https://www.uniprot.org/uniprotkb/P29017) | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | [BioLegend](https://www.biolegend.com/) | 331522 | AF488 | AB_10720182 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD1c_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P29017](https://www.uniprot.org/uniprotkb/P29017) | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | [AAT Bioquest](https://www.aatbio.com/) | 10013150 | AF555 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD1c_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P11609](https://www.uniprot.org/uniprotkb/P11609) | Primary Antibody | CD1d | Mouse | Rat | IgG2b | 1B1 | [BD Biosciences](https://www.bdbiosciences.com/) | 553846 | PE | AB_2073521 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/CD1d_PE/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | -| [P19437](https://www.uniprot.org/uniprotkb/P19437) | Primary Antibody | CD20 | Mouse | Rat | IgG2b | SA275A11 | [BioLegend](https://www.biolegend.com/) | 150432 | AF488 | AB_2890699 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | [0000-0002-1461-0999](supporting_material/CD20_AF488/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | -| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 42-0202-82 | eF615 | AB_10853517 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0001-9561-4256](supporting_material/CD20_eF615/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-0202-82 | eF660 | AB_11150959 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD20_eF660/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-0202-82 | AF488 | AB_10734358 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD20_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 (Sodium Citrate buffer) for 45 minutes | NA | Yes | [0000-0002-6604-2065](supporting_material/CD20_eF615/0000-0002-6604-2065.md) | NA | 0000-0002-6604-2065 | | [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-0202-82 | eF660 | AB_11150959 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/CD20_eF660/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | -| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 42-0202-82 | eF615 | AB_10853517 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0001-9561-4256](supporting_material/CD20_eF615/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-0202-82 | AF488 | AB_10734358 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD20_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD20_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [H9YXP1](https://www.uniprot.org/uniprotkb/H9YXP1) | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2b | 2H7 | [BioLegend](https://www.biolegend.com/) | 302354 | AF594 | AB_2565619 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD20_AF594/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-0202-82 | eF660 | AB_11150959 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD20_eF660/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/CD20_eF615/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-0202-82 | AF488 | AB_10734358 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD20_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [H9YXP1](https://www.uniprot.org/uniprotkb/H9YXP1) | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-0202-82 | AF488 | AB_10734358 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD20_AF488/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [H9YXP1](https://www.uniprot.org/uniprotkb/H9YXP1) | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-0202-82 | eF660 | AB_11150959 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD20_eF660/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [H9YXP1](https://www.uniprot.org/uniprotkb/H9YXP1) | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD20_eF615/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-0202-82 | AF488 | AB_10734358 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD20_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD20_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD20_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q3C2E2](https://www.uniprot.org/uniprotkb/Q3C2E2) | Primary Antibody | CD20 | Canine | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/CD20_eF615/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | -| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 (Sodium Citrate buffer) for 45 minutes | NA | Yes | [0000-0002-6604-2065](supporting_material/CD20_eF615/0000-0002-6604-2065.md) | NA | 0000-0002-6604-2065 | +| [H9YXP1](https://www.uniprot.org/uniprotkb/H9YXP1) | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-0202-82 | eF660 | AB_11150959 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | [0009-0004-2746-8743](supporting_material/CD20_eF660/0009-0004-2746-8743.md) | NA | 0009-0004-2746-8743 | +| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/CD20_eF615/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 42-0202-82 | eF615 | AB_10853517 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0001-9561-4256](supporting_material/CD20_eF615/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD20_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD20_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-80486AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD20_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD20_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-0202-82 | eF660 | AB_11150959 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD20_eF660/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-0202-82 | eF660 | AB_11150959 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD20_eF660/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P19437](https://www.uniprot.org/uniprotkb/P19437) | Primary Antibody | CD20 | Mouse | Rat | IgG2b | SA275A11 | [BioLegend](https://www.biolegend.com/) | 150432 | AF488 | AB_2890699 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | [0000-0002-1461-0999](supporting_material/CD20_AF488/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | +| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 42-0202-82 | eF615 | AB_10853517 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0001-9561-4256](supporting_material/CD20_eF615/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-80486AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD20_AF750/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-80486AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD20_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2b | 2H7 | [BioLegend](https://www.biolegend.com/) | 302322 | AF700 | AB_493753 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD20_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [H9YXP1](https://www.uniprot.org/uniprotkb/H9YXP1) | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-0202-82 | AF488 | AB_10734358 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD20_AF488/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [H9YXP1](https://www.uniprot.org/uniprotkb/H9YXP1) | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD20_eF615/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [Q3C2E2](https://www.uniprot.org/uniprotkb/Q3C2E2) | Primary Antibody | CD20 | Canine | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-0202-82 | eF660 | AB_11150959 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/CD20_eF660/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | -| [H9YXP1](https://www.uniprot.org/uniprotkb/H9YXP1) | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-0202-82 | eF660 | AB_11150959 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | [0009-0004-2746-8743](supporting_material/CD20_eF660/0009-0004-2746-8743.md) | NA | 0009-0004-2746-8743 | -| [H9YXP1](https://www.uniprot.org/uniprotkb/H9YXP1) | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-0202-82 | eF660 | AB_11150959 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD20_eF660/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P11836](https://www.uniprot.org/uniprotkb/P11836) | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-0202-82 | AF488 | AB_10734358 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD20_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q3C2E2](https://www.uniprot.org/uniprotkb/Q3C2E2) | Primary Antibody | CD20 | Canine | Mouse | IgG2a | L26 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/CD20_eF615/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | | [Q9ES57](https://www.uniprot.org/uniprotkb/Q9ES57) | Primary Antibody | CD200 | Mouse | Rat | IgG2a | OX-90 | [BD Biosciences](https://www.bdbiosciences.com/) | 565547 | BV421 | AB_2739289 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD200_BV421/0000-0003-4379-8967.md), [0000-0002-1461-0999](supporting_material/CD200_BV421/0000-0002-1461-0999.md) | NA | 0000-0003-4379-8967 | | [Q61830](https://www.uniprot.org/uniprotkb/Q61830) | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | [BioLegend](https://www.biolegend.com/) | 141717 | BV421 | AB_2562232 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD206_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P22897](https://www.uniprot.org/uniprotkb/P22897) | Primary Antibody | CD206 | Human | Mouse | IgG2b | 685645 | [R&D Systems](https://www.rndsystems.com/) | MAB25341 | Unconjugated | AB_10890782 | Stock | Multiplexed 2D Imaging | FFPE | Lung | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD206_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q61830](https://www.uniprot.org/uniprotkb/Q61830) | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | [BioLegend](https://www.biolegend.com/) | 141717 | BV421 | AB_2562232 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD206_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q61830](https://www.uniprot.org/uniprotkb/Q61830) | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | [BioLegend](https://www.biolegend.com/) | 141717 | BV421 | AB_2562232 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD206_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q61830](https://www.uniprot.org/uniprotkb/Q61830) | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | [BioLegend](https://www.biolegend.com/) | 141709 | AF488 | AB_10933252 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0001-5813-2581](supporting_material/CD206_AF488/0000-0001-5813-2581.md) | NA | 0000-0001-5813-2581 | +| [P22897](https://www.uniprot.org/uniprotkb/P22897) | Primary Antibody | CD206 | Human | Mouse | IgG2b | 685645 | [R&D Systems](https://www.rndsystems.com/) | MAB25341 | Unconjugated | AB_10890782 | Stock | Multiplexed 2D Imaging | FFPE | Lung | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD206_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P22897](https://www.uniprot.org/uniprotkb/P22897) | Primary Antibody | CD206 | Human | Mouse | IgG1 | 15-2 | [BioLegend](https://www.biolegend.com/) | 321130 | PE/Dazzle AF594 | AB_2616867 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/CD206_PE_Dazzle_AF594/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | | [Q8VBX4](https://www.uniprot.org/uniprotkb/Q8VBX4) | Primary Antibody | CD207 | Mouse | Rat | IgG2a | eBioL31 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 12-2075-80 | PE | AB_763453 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD207_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | [BioLegend](https://www.biolegend.com/) | 354903 | PE | AB_2561406 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD21_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | [BioLegend](https://www.biolegend.com/) | 354902 (Unconjugated) | AF532 | AB_2892739 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD21_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | [BioLegend](https://www.biolegend.com/) | 354902 (Unconjugated) | AF488 | NA | Custom | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD21_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | [BioLegend](https://www.biolegend.com/) | 354902 (Unconjugated) | AF532 | AB_2892739 | Custom | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD21_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | [BioLegend](https://www.biolegend.com/) | 354909 | FITC | AB_2561574 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD21_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | [Abcam](https://www.abcam.com/) | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD21_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | [BioLegend](https://www.biolegend.com/) | 354902 (Unconjugated) | AF488 | NA | Custom | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD21_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | [Abcam](https://www.abcam.com/) | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD21_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | [BioLegend](https://www.biolegend.com/) | 354902 (Unconjugated) | AF532 | AB_2892739 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD21_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | [Abcam](https://www.abcam.com/) | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | No | [0000-0003-4379-8967](supporting_material/CD21_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | CD21 | Mouse | Rat | IgG2a | 7.E.9 | [BioLegend](https://www.biolegend.com/) | 123413 | Pacific Blue | AB_2085159 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD21_Pacific_Blue/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Mouse | IgG1 | 1F8 | [Novus Biologicals](https://www.novusbio.com/) | NBP1-22527 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD21_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | [Abcam](https://www.abcam.com/) | ab240987 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD21_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | [Abcam](https://www.abcam.com/) | ab240987 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD21_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | [BioLegend](https://www.biolegend.com/) | 354903 | PE | AB_2561406 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD21_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | [BioLegend](https://www.biolegend.com/) | 354909 | FITC | AB_2561574 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD21_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Mouse | IgG1 | CR2/1952 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-60733AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD21_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Mouse | IgG1 | 1F8 | [Novus Biologicals](https://www.novusbio.com/) | NBP1-22527 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD21_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | CD21 | Mouse | Rat | IgG2a | 7.E.9 | [BioLegend](https://www.biolegend.com/) | 123413 | Pacific Blue | AB_2085159 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD21_Pacific_Blue/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | [Abcam](https://www.abcam.com/) | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD21_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | [Abcam](https://www.abcam.com/) | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | No | [0000-0003-4379-8967](supporting_material/CD21_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P20023](https://www.uniprot.org/uniprotkb/P20023) | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | [BioLegend](https://www.biolegend.com/) | 354902 (Unconjugated) | AF532 | AB_2892739 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD21_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P06734](https://www.uniprot.org/uniprotkb/P06734) | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | [BioLegend](https://www.biolegend.com/) | 338521 | BV421 | AB_2562460 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD23_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P06734](https://www.uniprot.org/uniprotkb/P06734) | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | [BioLegend](https://www.biolegend.com/) | 338505 | FITC | AB_1501125 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD23_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P06734](https://www.uniprot.org/uniprotkb/P06734) | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | [BioLegend](https://www.biolegend.com/) | 338521 | BV421 | AB_2562460 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD23_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P20693](https://www.uniprot.org/uniprotkb/P20693) | Primary Antibody | CD23 | Mouse | Rat | IgG2a | B3B4 | [BioLegend](https://www.biolegend.com/) | 101611 | AF647 | AB_493479 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD23_AF647/0000-0003-4379-8967.md), [0000-0002-8909-5039](supporting_material/CD23_AF647/0000-0002-8909-5039.md) | NA | 0000-0003-4379-8967 | +| [P06734](https://www.uniprot.org/uniprotkb/P06734) | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | [BioLegend](https://www.biolegend.com/) | 338502 (Unconjugated) | AF532 | AB_2892740 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD23_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P06734](https://www.uniprot.org/uniprotkb/P06734) | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | [BioLegend](https://www.biolegend.com/) | 338502 (Unconjugated) | AF532 | AB_2892740 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD23_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P06734](https://www.uniprot.org/uniprotkb/P06734) | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | [BioLegend](https://www.biolegend.com/) | 338502 (Unconjugated) | AF532 | AB_2892740 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD23_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P06734](https://www.uniprot.org/uniprotkb/P06734) | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | [BioLegend](https://www.biolegend.com/) | 338502 (Unconjugated) | AF532 | AB_2892740 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD23_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01590](https://www.uniprot.org/uniprotkb/P01590) | Primary Antibody | CD25 | Mouse | Rat | IgG1 | PC61.5 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-0251-82 | AF488 | AB_763472 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD25_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01589](https://www.uniprot.org/uniprotkb/P01589) | Primary Antibody | CD25 | Human | Mouse | IgG1 | M-A251 | [BioLegend](https://www.biolegend.com/) | 356128 | AF647 | AB_2563588 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD25_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01589](https://www.uniprot.org/uniprotkb/P01589) | Primary Antibody | CD25 | Human | Mouse | IgG2b | CD25-4E3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 17-0257-42 | APC | AB_11218671 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD25_APC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01590](https://www.uniprot.org/uniprotkb/P01590) | Primary Antibody | CD25 | Mouse | Rat | IgG1 | PC61.5 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-0251-82 | AF488 | AB_763472 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD25_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01589](https://www.uniprot.org/uniprotkb/P01589) | Primary Antibody | CD25 | Human | Mouse | IgG1 | M-A251 | [BioLegend](https://www.biolegend.com/) | 356128 | AF647 | AB_2563588 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD25_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01589](https://www.uniprot.org/uniprotkb/P01589) | Primary Antibody | CD25 | Human | Mouse | IgG2b | CD25-4E3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 17-0257-42 | APC | AB_11218671 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD25_APC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08138](https://www.uniprot.org/uniprotkb/P08138) | Primary Antibody | CD271 (NGFR5) | Human | Mouse | IgG1 | NGFR5 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA5-13311 | Biotin | AB_10982271 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD271__NGFR5__Biotin/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08138](https://www.uniprot.org/uniprotkb/P08138) | Primary Antibody | CD271 (NGFR5) | Human | Mouse | IgG1 | NGRF5 | [BioLegend](https://www.biolegend.com/) | 818201 | Unconjugated | AB_2564818 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD271__NGFR5__Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9EP73](https://www.uniprot.org/uniprotkb/Q9EP73) | Primary Antibody | CD274 (PD-L1) | Mouse | Rat | IgG2b | MIH1 | [BD Biosciences](https://www.bdbiosciences.com/) | 753201 | RY586 | NA | Stock | Ce3D | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | [0000-0002-0835-911X](supporting_material/CD274__PD-L1__RY586/0000-0002-0835-911X.md) | NA | 0000-0002-0835-911X | | [P09055](https://www.uniprot.org/uniprotkb/P09055) | Primary Antibody | CD29 | Mouse | Armenian Hamster | IgG | HMB1-1 | [BioLegend](https://www.biolegend.com/) | 102214 | AF647 | AB_492831 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-1461-0999](supporting_material/CD29_AF647/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | CAL57 | [Cell IDx](https://cellidx.com/) | HI06B-005 | UT016 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD3_UT016/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF555/0000-0003-4379-8967.md), [0009-0006-9784-2694](supporting_material/CD3_AF555/0009-0006-9784-2694.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | [Abcam](https://www.abcam.com/) | ab16669 | Unconjugated | AB_443425 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD3_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0038-42 | AF532 | AB_11218675 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EPR4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/CD3_AF555/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | -| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100210 | AF488 | AB_389301 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/CD3_AF488/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [AAT Bioquest](https://www.aatbio.com/) | 10032060 | iF514 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD3_iF514/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100214 | Pacific Blue | AB_493645 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/CD3_Pacific_Blue/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | | [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | [Abcam](https://www.abcam.com/) | ab16669 | Unconjugated | AB_443425 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD3_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [Novus Biologicals](https://www.novusbio.com/) | NB100-2726IR | DL755 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD3_DL755/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100205 | PE | AB_312662 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300448 | BV510 | AB_2563468 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD3_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P04234](https://www.uniprot.org/uniprotkb/P04234) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300483 | Spark Violet 538 | AB_2890733 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_Spark_Violet_538/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300415 | AF488 | AB_389310 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/CD3_AF488/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Skin | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4184-3867](supporting_material/CD3_AF555/0000-0003-4184-3867.md) | NA | 0000-0003-4184-3867 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | [Abcam](https://www.abcam.com/) | ab16669 | Unconjugated | AB_443425 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD3_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | CAL57 | [Cell IDx](https://cellidx.com/) | HI06B-005 | UT016 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD3_UT016/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Cervix | Cervical Cancer | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7586-9996](supporting_material/CD3_AF555/0000-0002-7586-9996.md) | NA | 0000-0002-7586-9996 | -| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100281 | Spark Red 718 | AB_2924440 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_Spark_Red_718/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100281 | Spark Red 718 | AB_2924440 | Stock | Ce3D | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | [0000-0002-5187-810X](supporting_material/CD3_Spark_Red_718/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | -| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0032-80 | AF532 | AB_11220474 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/CD3_AF532/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | -| [P04234](https://www.uniprot.org/uniprotkb/P04234) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300487 | Spark Blue 574 | AB_2904329 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_Spark_Blue_574/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300415 | AF488 | AB_389310 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100281 | Spark Red 718 | AB_2924440 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-1130-1899](supporting_material/CD3_Spark_Red_718/0000-0003-1130-1899.md) | NA | 0000-0003-1130-1899 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300408 | PE | AB_314062 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300402 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300416 | AF647 | AB_389332 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | [Abcam](https://www.abcam.com/) | ab16669 | Unconjugated | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD3_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100205 | PE | AB_312662 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100210 | AF488 | AB_389301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF488/0000-0003-4379-8967.md), [0000-0002-1461-0999](supporting_material/CD3_AF488/0000-0002-1461-0999.md) | NA | 0000-0003-4379-8967 | -| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100234 | BV510 | AB_2562555 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD3_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | [Abcam](https://www.abcam.com/) | ab16669 | Unconjugated | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD3_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100228 | BV421 | AB_2562553 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD3_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [G7NCB9](https://www.uniprot.org/uniprotkb/G7NCB9) | Primary Antibody | CD3 | Rhesus Macaque | Mouse | IgG1 | F7.2.38 | [Agilent](https://www.agilent.com/en/dako-products) | M7254 | Unconjugated | AB_2631163 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD3_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Skin | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4184-3867](supporting_material/CD3_AF555/0000-0003-4184-3867.md) | NA | 0000-0003-4184-3867 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300415 | AF488 | AB_389310 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/CD3_AF488/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [AAT Bioquest](https://www.aatbio.com/) | 10032060 | iF514 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD3_iF514/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P27597](https://www.uniprot.org/uniprotkb/P27597) | Primary Antibody | CD3 | Canine | Rabbit | IgG | SP7 | [Abcam](https://www.abcam.com/) | ab16669 | Unconjugated | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0001-5088-7808](supporting_material/CD3_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [Novus Biologicals](https://www.novusbio.com/) | NB100-2726IR | DL755 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD3_DL755/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300446 | AF594 | AB_2563236 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300448 | BV510 | AB_2563468 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD3_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF555/0000-0003-4379-8967.md), [0009-0006-9784-2694](supporting_material/CD3_AF555/0009-0006-9784-2694.md) | NA | 0000-0003-4379-8967 | | [P04234](https://www.uniprot.org/uniprotkb/P04234) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300401 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P04234](https://www.uniprot.org/uniprotkb/P04234) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300435 | BV570 | AB_10898117 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD3_BV570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300448 | BV510 | AB_2563468 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD3_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BD Biosciences](https://www.bdbiosciences.com/) | 557869 | AF647 | AB_396912 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P04234](https://www.uniprot.org/uniprotkb/P04234) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300487 | Spark Blue 574 | AB_2904329 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_Spark_Blue_574/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100210 | AF488 | AB_389301 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/CD3_AF488/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | +| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0032-80 | AF532 | AB_11220474 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/CD3_AF532/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | +| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100281 | Spark Red 718 | AB_2924440 | Stock | Ce3D | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | [0000-0002-5187-810X](supporting_material/CD3_Spark_Red_718/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | +| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100214 | Pacific Blue | AB_493645 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/CD3_Pacific_Blue/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | +| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100281 | Spark Red 718 | AB_2924440 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_Spark_Red_718/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300446 | AF594 | AB_2563236 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [G7NCB9](https://www.uniprot.org/uniprotkb/G7NCB9) | Primary Antibody | CD3 | Rhesus Macaque | Mouse | IgG1 | F7.2.38 | [Agilent](https://www.agilent.com/en/dako-products) | M7254 | Unconjugated | AB_2631163 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD3_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100281 | Spark Red 718 | AB_2924440 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-1130-1899](supporting_material/CD3_Spark_Red_718/0000-0003-1130-1899.md) | NA | 0000-0003-1130-1899 | +| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BD Biosciences](https://www.bdbiosciences.com/) | 557869 | AF647 | AB_396912 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BD Biosciences](https://www.bdbiosciences.com/) | 557869 | AF647 | AB_396912 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD3_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300446 | AF594 | AB_2563236 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0038-42 | AF532 | AB_11218675 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD3_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD3_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD3_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD3_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EPR4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/CD3_AF555/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | | [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100240 | AF594 | AB_2563427 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100240 | AF594 | AB_2563427 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF594/0000-0003-4379-8967.md), [0000-0003-1118-7432](supporting_material/CD3_AF594/0000-0003-1118-7432.md) | NA | 0000-0003-4379-8967 | -| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | C31.3+C31.7+C31.10 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47785AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | C31.3+C31.7+C31.10 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47785AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303126 | AF594 | AB_2563303 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303102 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100228 | BV421 | AB_2562553 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD3_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | [Abcam](https://www.abcam.com/) | ab16669 | Unconjugated | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD3_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300416 | AF647 | AB_389332 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300402 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300408 | PE | AB_314062 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | [Abcam](https://www.abcam.com/) | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100234 | BV510 | AB_2562555 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD3_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | [BioLegend](https://www.biolegend.com/) | 100210 | AF488 | AB_389301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF488/0000-0003-4379-8967.md), [0000-0002-1461-0999](supporting_material/CD3_AF488/0000-0002-1461-0999.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300415 | AF488 | AB_389310 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD3_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | [BioLegend](https://www.biolegend.com/) | 300448 | BV510 | AB_2563468 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD3_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07766](https://www.uniprot.org/uniprotkb/P07766) | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | [Abcam](https://www.abcam.com/) | ab16669 | Unconjugated | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD3_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303133 | AF700 | AB_2566326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q08481](https://www.uniprot.org/uniprotkb/Q08481) | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | [BioLegend](https://www.biolegend.com/) | 102514 | AF488 | AB_2161031 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [A0A5F7Z8X0](https://www.uniprot.org/uniprotkb/A0A5F7Z8X0) | Primary Antibody | CD31 | Rhesus Macaque | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab28364 | Unconjugated | AB_726362 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD31_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303106 | PE | AB_314332 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD31_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q08481](https://www.uniprot.org/uniprotkb/Q08481) | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | [BioLegend](https://www.biolegend.com/) | 102515 | AF647 | AB_2161030 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](supporting_material/CD31_AF647/0000-0002-6944-6997.md) | NA | 0000-0002-6944-6997 | | [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303110 | AF488 | AB_493074 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303102 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303126 | AF594 | AB_2563303 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303112 | AF647 | AB_493076 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | C31.3+C31.7+C31.10 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47785AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | C31.3+C31.7+C31.10 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47785AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q08481](https://www.uniprot.org/uniprotkb/Q08481) | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | [BD Biosciences](https://www.bdbiosciences.com/) | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD31_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303106 | PE | AB_314332 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD31_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303133 | AF700 | AB_2566326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q08481](https://www.uniprot.org/uniprotkb/Q08481) | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | [BioLegend](https://www.biolegend.com/) | 102520 | AF594 | AB_2563319 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q08481](https://www.uniprot.org/uniprotkb/Q08481) | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | [BioLegend](https://www.biolegend.com/) | 102520 | AF594 | AB_2563319 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0002-1461-0999](supporting_material/CD31_AF594/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | -| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303112 | AF647 | AB_493076 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q08481](https://www.uniprot.org/uniprotkb/Q08481) | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | [BD Biosciences](https://www.bdbiosciences.com/) | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD31_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303133 | AF700 | AB_2566326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q08481](https://www.uniprot.org/uniprotkb/Q08481) | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | [BD Biosciences](https://www.bdbiosciences.com/) | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/CD31_PE/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | +| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | C31.3+C31.7+C31.10 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47785AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q08481](https://www.uniprot.org/uniprotkb/Q08481) | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | [BioLegend](https://www.biolegend.com/) | 102520 | AF594 | AB_2563319 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0002-1461-0999](supporting_material/CD31_AF594/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | | [Q08481](https://www.uniprot.org/uniprotkb/Q08481) | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | [BD Biosciences](https://www.bdbiosciences.com/) | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD31_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q08481](https://www.uniprot.org/uniprotkb/Q08481) | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | [BioLegend](https://www.biolegend.com/) | 102520 | AF594 | AB_2563319 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0002-7422-3237](supporting_material/CD31_AF594/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | | [Q08481](https://www.uniprot.org/uniprotkb/Q08481) | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | [BioLegend](https://www.biolegend.com/) | 102516 | AF647 | AB_2161029 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q08481](https://www.uniprot.org/uniprotkb/Q08481) | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | [BioLegend](https://www.biolegend.com/) | 102520 | AF594 | AB_2563319 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/CD31_AF594/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | -| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | C31.3+C31.7+C31.10 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47785AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303133 | AF700 | AB_2566326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD31_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303106 | PE | AB_314332 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD31_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q08481](https://www.uniprot.org/uniprotkb/Q08481) | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | [BioLegend](https://www.biolegend.com/) | 102515 | AF647 | AB_2161030 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](supporting_material/CD31_AF647/0000-0002-6944-6997.md) | NA | 0000-0002-6944-6997 | -| [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303106 | PE | AB_314332 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD31_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303106 | PE | AB_314332 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD31_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q08481](https://www.uniprot.org/uniprotkb/Q08481) | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | [BioLegend](https://www.biolegend.com/) | 102520 | AF594 | AB_2563319 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/CD31_AF594/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | | [P16284](https://www.uniprot.org/uniprotkb/P16284) | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | [BioLegend](https://www.biolegend.com/) | 303106 | PE | AB_314332 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD31_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [A0A5F7Z8X0](https://www.uniprot.org/uniprotkb/A0A5F7Z8X0) | Primary Antibody | CD31 | Rhesus Macaque | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab28364 | Unconjugated | AB_726362 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD31_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P28906](https://www.uniprot.org/uniprotkb/P28906) | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | [Novus Biologicals](https://www.novusbio.com/) | FAB7227T | AF594 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/CD34_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P28906](https://www.uniprot.org/uniprotkb/P28906) | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | [AAT Bioquest](https://www.aatbio.com/) | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD34_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P28906](https://www.uniprot.org/uniprotkb/P28906) | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA1-10205 | PE | AB_11152571 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD34_PE/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P28906](https://www.uniprot.org/uniprotkb/P28906) | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA1-10205 | PE | AB_11152571 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD34_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q08481](https://www.uniprot.org/uniprotkb/Q08481) | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | [BioLegend](https://www.biolegend.com/) | 102520 | AF594 | AB_2563319 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0002-7422-3237](supporting_material/CD31_AF594/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | +| [Q08481](https://www.uniprot.org/uniprotkb/Q08481) | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | [BD Biosciences](https://www.bdbiosciences.com/) | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/CD31_PE/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | +| [P28906](https://www.uniprot.org/uniprotkb/P28906) | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | [AAT Bioquest](https://www.aatbio.com/) | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD34_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P28906](https://www.uniprot.org/uniprotkb/P28906) | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | [AAT Bioquest](https://www.aatbio.com/) | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD34_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P28906](https://www.uniprot.org/uniprotkb/P28906) | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | [Novus Biologicals](https://www.novusbio.com/) | FAB7227T | AF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD34_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P28906](https://www.uniprot.org/uniprotkb/P28906) | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | [AAT Bioquest](https://www.aatbio.com/) | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD34_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P28906](https://www.uniprot.org/uniprotkb/P28906) | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | [AAT Bioquest](https://www.aatbio.com/) | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD34_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P28906](https://www.uniprot.org/uniprotkb/P28906) | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA1-10205 | PE | AB_11152571 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD34_PE/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P28906](https://www.uniprot.org/uniprotkb/P28906) | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | [AAT Bioquest](https://www.aatbio.com/) | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD34_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P28906](https://www.uniprot.org/uniprotkb/P28906) | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | [Novus Biologicals](https://www.novusbio.com/) | FAB7227T | AF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD34_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P28906](https://www.uniprot.org/uniprotkb/P28906) | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA1-10205 | PE | AB_11152571 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD34_PE/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P28906](https://www.uniprot.org/uniprotkb/P28906) | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | [Novus Biologicals](https://www.novusbio.com/) | FAB7227T | AF594 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/CD34_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P28906](https://www.uniprot.org/uniprotkb/P28906) | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA1-10205 | PE | AB_11152571 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0009-0006-9784-2694](supporting_material/CD34_PE/0009-0006-9784-2694.md) | NA | 0009-0006-9784-2694 | +| [P28906](https://www.uniprot.org/uniprotkb/P28906) | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA1-10205 | PE | AB_11152571 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD34_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P17927](https://www.uniprot.org/uniprotkb/P17927) | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | [BioLegend](https://www.biolegend.com/) | 333404 | FITC | AB_2085022 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD35_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P17927](https://www.uniprot.org/uniprotkb/P17927) | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | [BioLegend](https://www.biolegend.com/) | 333406 | PE | AB_2292231 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD35_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P17927](https://www.uniprot.org/uniprotkb/P17927) | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | [BioLegend](https://www.biolegend.com/) | 333406 | PE | AB_2292231 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD35_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P17927](https://www.uniprot.org/uniprotkb/P17927) | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | [BD Biosciences](https://www.bdbiosciences.com/) | 565329 | AF647 | AB_2739186 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD35_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | [BD Biosciences](https://www.bdbiosciences.com/) | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD35_BV510/0000-0003-4379-8967.md), [0000-0003-1118-7432](supporting_material/CD35_BV510/0000-0003-1118-7432.md), [0000-0002-1461-0999](supporting_material/CD35_BV510/0000-0002-1461-0999.md) | NA | 0000-0003-4379-8967 | | [P17927](https://www.uniprot.org/uniprotkb/P17927) | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | [BioLegend](https://www.biolegend.com/) | 333406 | PE | AB_2292231 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD35_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P17927](https://www.uniprot.org/uniprotkb/P17927) | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | [BioLegend](https://www.biolegend.com/) | 333406 | PE | AB_2292231 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD35_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P17927](https://www.uniprot.org/uniprotkb/P17927) | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | [BioLegend](https://www.biolegend.com/) | 333406 | PE | AB_2292231 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD35_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | [BD Biosciences](https://www.bdbiosciences.com/) | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD35_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | [BD Biosciences](https://www.bdbiosciences.com/) | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD35_BV510/0000-0003-4379-8967.md), [0000-0003-1118-7432](supporting_material/CD35_BV510/0000-0003-1118-7432.md), [0000-0002-1461-0999](supporting_material/CD35_BV510/0000-0002-1461-0999.md) | NA | 0000-0003-4379-8967 | -| [P56528](https://www.uniprot.org/uniprotkb/P56528) | Primary Antibody | CD38 | Mouse | Rat | IgG2a | 90 | [BioLegend](https://www.biolegend.com/) | 102732 | BV421 | AB_2734153 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0002-1461-0999](supporting_material/CD38_BV421/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | -| [P28907](https://www.uniprot.org/uniprotkb/P28907) | Primary Antibody | CD38 | Human | Mouse | IgG1 | HIT2 | [BioLegend](https://www.biolegend.com/) | 303524 | AF700 | AB_2072781 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD38_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P28907](https://www.uniprot.org/uniprotkb/P28907) | Primary Antibody | CD38 | Human | Mouse | IgG1 | HIT2 | [BioLegend](https://www.biolegend.com/) | 303503 | FITC | AB_314355 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD38_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P28907](https://www.uniprot.org/uniprotkb/P28907) | Primary Antibody | CD38 | Human | Mouse | IgG1 | HIT2 | [BioLegend](https://www.biolegend.com/) | 303524 | AF700 | AB_2072781 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD38_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P56528](https://www.uniprot.org/uniprotkb/P56528) | Primary Antibody | CD38 | Mouse | Rat | IgG2a | 90 | [BioLegend](https://www.biolegend.com/) | 102732 | BV421 | AB_2734153 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0002-1461-0999](supporting_material/CD38_BV421/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | | [P55772](https://www.uniprot.org/uniprotkb/P55772) | Primary Antibody | CD39 | Human | Mouse | IgG1 | A1 | [BioLegend](https://www.biolegend.com/) | 328208 | PE | AB_940429 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD39_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P55772](https://www.uniprot.org/uniprotkb/P55772) | Primary Antibody | CD39 | Human | Mouse | IgG1 | A1 | [BioLegend](https://www.biolegend.com/) | 328205 | FITC | AB_940423 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD39_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P55772](https://www.uniprot.org/uniprotkb/P55772) | Primary Antibody | CD39 | Human | Mouse | IgG1 | A1 | [BioLegend](https://www.biolegend.com/) | 328205 | FITC | AB_940423 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD39_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3e | Human | Mouse | IgG1 | UCHT1 | [Bio-Rad](https://www.bio-rad.com/) | MCA463SBV670 | StarBright Violet 670 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-0835-911X](supporting_material/CD3e_StarBright_Violet_670/0000-0002-0835-911X.md) | NA | 0000-0002-0835-911X | | [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3e | Human | Mouse | IgG1 | UCHT1 | [Bio-Rad](https://www.bio-rad.com/) | MCA463SBV710 | StarBright Violet 710 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-0835-911X](supporting_material/CD3e_StarBright_Violet_710/0000-0002-0835-911X.md) | NA | 0000-0002-0835-911X | | [P22646](https://www.uniprot.org/uniprotkb/P22646) | Primary Antibody | CD3e | Human | Mouse | IgG1 | UCHT1 | [Bio-Rad](https://www.bio-rad.com/) | MCA463SBB700 | StarBright Blue 700 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-0835-911X](supporting_material/CD3e_StarBright_Blue_700/0000-0002-0835-911X.md) | NA | 0000-0002-0835-911X | -| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-27216AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD4_AF750/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | [BioLegend](https://www.biolegend.com/) | 100533 | AF647 | AB_493372 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Spleen | Healthy | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](supporting_material/CD4_AF647/0000-0002-6944-6997.md) | NA | 0000-0002-6944-6997 | -| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0042-80 | AF532 | AB_11219484 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | [BioLegend](https://www.biolegend.com/) | 300520 | AF647 | AB_389333 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16003](https://www.uniprot.org/uniprotkb/P16003) | Primary Antibody | CD4 | Rhesus Macaque | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | FAB8165N | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD4_AF700/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA1211 | [Miltenyi Biotec](https://www.miltenyibiotec.com/) | 130-123-209 | VioGreen | AB_2811462 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-6980-8900](supporting_material/CD4_VioGreen/0000-0002-6980-8900.md) | NA | 0000-0002-6980-8900 | +| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA1211 | [Miltenyi Biotec](https://www.miltenyibiotec.com/) | 130-128-234 | Vio Bright R720 | AB_2905066 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-6980-8900](supporting_material/CD4_Vio_Bright_R720/0000-0002-6980-8900.md) | NA | 0000-0002-6980-8900 | | [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | [AAT Bioquest](https://www.aatbio.com/) | 10041090 | iF555 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_iF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | [Abcam](https://www.abcam.com/) | ab196372 | AF488 | AB_2889191 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | [Abcam](https://www.abcam.com/) | ab196372 | AF488 | AB_2889191 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | [BD Biosciences](https://www.bdbiosciences.com/) | 553730 | PE | AB_396634 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | [BioLegend](https://www.biolegend.com/) | 100533 | AF647 | AB_493372 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Spleen | Healthy | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](supporting_material/CD4_AF647/0000-0002-6944-6997.md) | NA | 0000-0002-6944-6997 | | [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | [Miltenyi Biotec](https://www.miltenyibiotec.com/) | 130-118-568 | VioBlue | AB_2733943 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | [0000-0002-6980-8900](supporting_material/CD4_VioBlue/0000-0002-6980-8900.md) | NA | 0000-0002-6980-8900 | +| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | [Abcam](https://www.abcam.com/) | ab196372 | AF488 | AB_2889191 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | [BioLegend](https://www.biolegend.com/) | 100533 | AF647 | AB_493372 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](supporting_material/CD4_AF647/0000-0002-6944-6997.md) | NA | 0000-0002-6944-6997 | +| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | [Abcam](https://www.abcam.com/) | ab196372 | AF488 | AB_2889191 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P16003](https://www.uniprot.org/uniprotkb/P16003) | Primary Antibody | CD4 | Rhesus Macaque | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | FAB8165G | AF488 | AB_2728839 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD4_AF488/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | [Abcam](https://www.abcam.com/) | ab196372 | AF488 | AB_2889191 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | [BioLegend](https://www.biolegend.com/) | 100443 | BV421 | AB_2562557 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0002-1461-0999](supporting_material/CD4_BV421/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | -| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2a | GK1.5 | [BioLegend](https://www.biolegend.com/) | 100446 | AF594 | AB_2563182 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16003](https://www.uniprot.org/uniprotkb/P16003) | Primary Antibody | CD4 | Rhesus Macaque | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | FAB8165N | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CD4_AF700/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | [BioLegend](https://www.biolegend.com/) | 100443 | BV421 | AB_2562557 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-1431-1398](supporting_material/CD4_BV421/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | +| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF-379-SP | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD4_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | FAB8165G | AF488 | AB_2728839 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 (Sodium Citrate buffer) for 45 minutes | NA | Yes | [0000-0002-6604-2065](supporting_material/CD4_AF488/0000-0002-6604-2065.md) | NA | 0000-0002-6604-2065 | +| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-0042-82 | eF570 | AB_2573595 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-27216AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD4_AF750/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | FAB8165G | AF488 | AB_2728839 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD4_AF488/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0042-80 | AF532 | AB_11219484 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | [Abcam](https://www.abcam.com/) | ab196372 | AF488 | AB_2889191 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2a | GK1.5 | [BioLegend](https://www.biolegend.com/) | 100446 | AF594 | AB_2563182 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | [Miltenyi Biotec](https://www.miltenyibiotec.com/) | 130-118-794 | PerCP-Vio 700 | AB_2733032 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-6980-8900](supporting_material/CD4_PerCP-Vio_700/0000-0002-6980-8900.md) | NA | 0000-0002-6980-8900 | -| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | [Miltenyi Biotec](https://www.miltenyibiotec.com/) | 130-119-132 | APC-Vio 770 | AB_2733118 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6980-8900](supporting_material/CD4_APC-Vio_770/0000-0002-6980-8900.md) | NA | 0000-0002-6980-8900 | -| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | [Miltenyi Biotec](https://www.miltenyibiotec.com/) | 130-118-316 | PE-Vio 615 | AB_2728077 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6980-8900](supporting_material/CD4_PE-Vio_615/0000-0002-6980-8900.md) | NA | 0000-0002-6980-8900 | -| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA1211 | [Miltenyi Biotec](https://www.miltenyibiotec.com/) | 130-123-209 | VioGreen | AB_2811462 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-6980-8900](supporting_material/CD4_VioGreen/0000-0002-6980-8900.md) | NA | 0000-0002-6980-8900 | -| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA1211 | [Miltenyi Biotec](https://www.miltenyibiotec.com/) | 130-128-234 | Vio Bright R720 | AB_2905066 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-6980-8900](supporting_material/CD4_Vio_Bright_R720/0000-0002-6980-8900.md) | NA | 0000-0002-6980-8900 | -| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | [BD Biosciences](https://www.bdbiosciences.com/) | 557667 | AF488 | AB_396779 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | [BioLegend](https://www.biolegend.com/) | 100449 | BV510 | AB_2564587 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD4_BV510/0000-0003-4379-8967.md) | [0000-0002-1461-0999](supporting_material/CD4_BV510/0000-0002-1461-0999.md) | 0000-0003-4379-8967 | -| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | [BioLegend](https://www.biolegend.com/) | 100443 | BV421 | AB_2562557 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-1431-1398](supporting_material/CD4_BV421/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | -| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | FAB8165G | AF488 | AB_2728839 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD4_AF488/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | [Cell IDx](https://cellidx.com/) | HI06B-005 | UT019 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD4_UT019/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | CD4 | Canine | Rat | IgG2a | YKIX302.9 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA5-28357 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/CD4_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | -| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0049-42 | AF532 | AB_2802361 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0042-80 | AF532 | AB_11219484 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | [BioLegend](https://www.biolegend.com/) | 300520 | AF647 | AB_389333 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0049-42 | AF532 | AB_2802361 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | [BioLegend](https://www.biolegend.com/) | 300520 | AF647 | AB_389333 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | [BioLegend](https://www.biolegend.com/) | 300520 | AF647 | AB_389333 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF-379-SP | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD4_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | [BioLegend](https://www.biolegend.com/) | 300520 | AF647 | AB_389333 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0049-42 | AF532 | AB_2802361 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0049-42 | AF532 | AB_2802361 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | CD4 | Canine | Rat | IgG2a | YKIX302.9 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA5-28357 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/CD4_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | | [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF-379-SP | Unconjugated | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0002-2692-1225](supporting_material/CD4_Unconjugated/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | | [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | [BioLegend](https://www.biolegend.com/) | 300526 | AF700 | AB_493743 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Metastatic | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0042-80 | AF532 | AB_11219484 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | [Abcam](https://www.abcam.com/) | ab196372 | AF488 | AB_2889191 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | FAB8165G | AF488 | AB_2728839 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD4_AF488/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | [BioLegend](https://www.biolegend.com/) | 100443 | BV421 | AB_2562557 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0002-1461-0999](supporting_material/CD4_BV421/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | +| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | [BioLegend](https://www.biolegend.com/) | 100449 | BV510 | AB_2564587 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD4_BV510/0000-0003-4379-8967.md) | [0000-0002-1461-0999](supporting_material/CD4_BV510/0000-0002-1461-0999.md) | 0000-0003-4379-8967 | +| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | [BD Biosciences](https://www.bdbiosciences.com/) | 553730 | PE | AB_396634 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | [Miltenyi Biotec](https://www.miltenyibiotec.com/) | 130-118-316 | PE-Vio 615 | AB_2728077 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6980-8900](supporting_material/CD4_PE-Vio_615/0000-0002-6980-8900.md) | NA | 0000-0002-6980-8900 | | [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | [BioLegend](https://www.biolegend.com/) | 300519 | AF488 | AB_389311 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | FAB8165G | AF488 | AB_2728839 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 (Sodium Citrate buffer) for 45 minutes | NA | Yes | [0000-0002-6604-2065](supporting_material/CD4_AF488/0000-0002-6604-2065.md) | NA | 0000-0002-6604-2065 | -| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-0042-82 | eF570 | AB_2573595 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | [BioLegend](https://www.biolegend.com/) | 100533 | AF647 | AB_493372 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](supporting_material/CD4_AF647/0000-0002-6944-6997.md) | NA | 0000-0002-6944-6997 | -| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | [BioLegend](https://www.biolegend.com/) | 300520 | AF647 | AB_389333 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | [BD Biosciences](https://www.bdbiosciences.com/) | 557667 | AF488 | AB_396779 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | [Miltenyi Biotec](https://www.miltenyibiotec.com/) | 130-123-894 | PE-Vio 770 | AB_2811571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | [0000-0002-6980-8900](supporting_material/CD4_PE-Vio_770/0000-0002-6980-8900.md) | NA | 0000-0002-6980-8900 | -| [P15379](https://www.uniprot.org/uniprotkb/P15379) | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P15379](https://www.uniprot.org/uniprotkb/P15379) | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P15379](https://www.uniprot.org/uniprotkb/P15379) | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103026 | AF700 | AB_493713 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/CD44_AF700/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | -| [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103007 | PE | AB_312958 | Stock | Multiplexed 2D Imaging | FFPE | Thymus | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD44_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01730](https://www.uniprot.org/uniprotkb/P01730) | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | [Abcam](https://www.abcam.com/) | ab196372 | AF488 | AB_2889191 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD4_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P06332](https://www.uniprot.org/uniprotkb/P06332) | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | [Miltenyi Biotec](https://www.miltenyibiotec.com/) | 130-119-132 | APC-Vio 770 | AB_2733118 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6980-8900](supporting_material/CD4_APC-Vio_770/0000-0002-6980-8900.md) | NA | 0000-0002-6980-8900 | +| [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103016 | AF488 | AB_493679 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103016 | AF488 | AB_493679 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103002 (Unconjugated) | AF532 | AB_2892768 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103016 | AF488 | AB_493679 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103002 (Unconjugated) | AF532 | AB_2892768 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P15379](https://www.uniprot.org/uniprotkb/P15379) | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103002 (Unconjugated) | AF532 | AB_2892768 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103054 | AF594 | AB_2563183 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103074 | Spark YG 570 | AB_2892265 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD44_Spark_YG_570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P15379](https://www.uniprot.org/uniprotkb/P15379) | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103002 (Unconjugated) | AF532 | AB_2892768 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [AAT Bioquest](https://www.aatbio.com/) | 104410C0 | iF594 | AB_2935685 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/CD44_iF594/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | +| [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103002 (Unconjugated) | AF532 | AB_2892768 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103016 | AF488 | AB_493679 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103002 (Unconjugated) | AF532 | AB_2892768 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103016 | AF488 | AB_493679 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103054 | AF594 | AB_2563183 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1016184 | PE/iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD45_PE_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P15379](https://www.uniprot.org/uniprotkb/P15379) | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P15379](https://www.uniprot.org/uniprotkb/P15379) | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103007 | PE | AB_312958 | Stock | Multiplexed 2D Imaging | FFPE | Thymus | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD44_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P15379](https://www.uniprot.org/uniprotkb/P15379) | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103026 | AF700 | AB_493713 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/CD44_AF700/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | +| [P16070](https://www.uniprot.org/uniprotkb/P16070) | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | [BioLegend](https://www.biolegend.com/) | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD44_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Uterus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2634-6838](supporting_material/CD45_AF700/0000-0003-2634-6838.md) | NA | 0000-0003-2634-6838 | -| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [Novus Biologicals](https://www.novusbio.com/) | NB100-77417IR | DL755 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Lung | Infected | Akoya Antibody Diluent | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0001-9189-7172](supporting_material/CD45_DL755/0000-0001-9189-7172.md) | NA | 0000-0001-9189-7172 | -| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0459-41 | AF532 | AB_11218084 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-34528PE | PE | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0009-0006-9784-2694](supporting_material/CD45_PE/0009-0006-9784-2694.md) | NA | 0009-0006-9784-2694 | -| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103172 | Spark YG 570 | AB_2860598 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment | Yes | [0000-0003-4379-8967](supporting_material/CD45_Spark_YG_570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-34528AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0009-0006-9784-2694](supporting_material/CD45_AF488/0009-0006-9784-2694.md) | NA | 0009-0006-9784-2694 | -| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-34528AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | [BioLegend](https://www.biolegend.com/) | 304060 | AF594 | AB_2629599 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0002-3882-457X](supporting_material/CD45_AF594/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | +| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103122 | AF488 | AB_493531 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-34528PE | PE | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/CD45_PE/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | -| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Infected | Akoya Antibody Diluent | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0001-9189-7172](supporting_material/CD45_AF700/0000-0001-9189-7172.md) | NA | 0000-0001-9189-7172 | -| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD45_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103134 | BV421 | AB_2562559 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD45_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103122 | AF488 | AB_493531 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103138 | BV510 | AB_2563061 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD45_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103124 | AF647 | AB_493533 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11 + PD7/26 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-34528AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD45_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD45_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/CD45_AF700/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | -| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0451-82 | AF532 | AB_11218871 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD45_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103122 | AF488 | AB_493531 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0459-41 | AF532 | AB_11218084 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1016184 | PE/iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD45_PE_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0451-82 | AF532 | AB_11218871 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0459-41 | AF532 | AB_11218084 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Metastatic | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | [BioLegend](https://www.biolegend.com/) | 304060 | AF594 | AB_2629599 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0002-3882-457X](supporting_material/CD45_AF594/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | +| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Infected | Akoya Antibody Diluent | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0001-9189-7172](supporting_material/CD45_AF700/0000-0001-9189-7172.md) | NA | 0000-0001-9189-7172 | +| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103144 | AF594 | AB_2563458 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0002-1461-0999](supporting_material/CD45_AF594/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | +| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [Novus Biologicals](https://www.novusbio.com/) | NB100-77417IR | DL755 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Lung | Infected | Akoya Antibody Diluent | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0001-9189-7172](supporting_material/CD45_DL755/0000-0001-9189-7172.md) | NA | 0000-0001-9189-7172 | | [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103134 | BV421 | AB_2562559 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD45_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103124 | AF647 | AB_493533 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103172 | Spark YG 570 | AB_2860598 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment | Yes | [0000-0003-4379-8967](supporting_material/CD45_Spark_YG_570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD45_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | [BioLegend](https://www.biolegend.com/) | 304018 | AF647 | AB_389336 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103144 | AF594 | AB_2563458 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0002-1461-0999](supporting_material/CD45_AF594/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | +| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103134 | BV421 | AB_2562559 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD45_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103144 | AF594 | AB_2563458 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0001-5813-2581](supporting_material/CD45_AF594/0000-0001-5813-2581.md) | NA | 0000-0001-5813-2581 | +| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103138 | BV510 | AB_2563061 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD45_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD45_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD45_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/CD45_AF700/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | +| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11 + PD7/26 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-34528AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-34528AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P06800](https://www.uniprot.org/uniprotkb/P06800) | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | [BioLegend](https://www.biolegend.com/) | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD45_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD45_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08575](https://www.uniprot.org/uniprotkb/P08575) | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1016184 | PE/iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD45_PE_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | CD45.1 | Mouse | Mouse | IgG2a | A20 | [BioLegend](https://www.biolegend.com/) | 110718 | AF488 | AB_492862 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-8909-5039](supporting_material/CD45.1_AF488/0000-0002-8909-5039.md) | NA | 0000-0002-8909-5039 | | NA | Primary Antibody | CD45RA | Human | Mouse | IgG1 | 5H9 | [BD Biosciences](https://www.bdbiosciences.com/) | 556626 | FITC | AB_396498 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD45RA_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | [BioLegend](https://www.biolegend.com/) | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD49a_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | [BioLegend](https://www.biolegend.com/) | 328308 | FITC | AB_2129084 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD49a_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | [BioLegend](https://www.biolegend.com/) | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD49a_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | [BioLegend](https://www.biolegend.com/) | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD49a_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | [BioLegend](https://www.biolegend.com/) | 328303 | PE | AB_1236407 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-3882-457X](supporting_material/CD49a_PE/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | -| NA | Primary Antibody | CD49a | Rhesus Macaque | Mouse | IgG1 | CL7207 | [Abcam](https://www.abcam.com/) | ab243032 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | [0009-0004-2746-8743](supporting_material/CD49a_Unconjugated/0009-0004-2746-8743.md) | NA | 0009-0004-2746-8743 | -| [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | CL7207 | [Abcam](https://www.abcam.com/) | ab243032 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD49a_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | CL7207 | [Abcam](https://www.abcam.com/) | ab243032 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD49a_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | [BioLegend](https://www.biolegend.com/) | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/CD49a_AF647/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | [BioLegend](https://www.biolegend.com/) | 328303 | PE | AB_1236441 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD49a_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | CL7207 | [Abcam](https://www.abcam.com/) | ab243032 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD49a_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | [BioLegend](https://www.biolegend.com/) | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD49a_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | [BioLegend](https://www.biolegend.com/) | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/CD49a_AF647/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | +| NA | Primary Antibody | CD49a | Rhesus Macaque | Mouse | IgG1 | CL7207 | [Abcam](https://www.abcam.com/) | ab243032 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | [0009-0004-2746-8743](supporting_material/CD49a_Unconjugated/0009-0004-2746-8743.md) | NA | 0009-0004-2746-8743 | +| [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | [BioLegend](https://www.biolegend.com/) | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD49a_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | [BioLegend](https://www.biolegend.com/) | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD49a_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | CL7207 | [Abcam](https://www.abcam.com/) | ab243032 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD49a_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | [BioLegend](https://www.biolegend.com/) | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD49a_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P05362](https://www.uniprot.org/uniprotkb/P05362) | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | [BioLegend](https://www.biolegend.com/) | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD54_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P05362](https://www.uniprot.org/uniprotkb/P05362) | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | [BioLegend](https://www.biolegend.com/) | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD54_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P05362](https://www.uniprot.org/uniprotkb/P05362) | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | [BioLegend](https://www.biolegend.com/) | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD54_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P56199](https://www.uniprot.org/uniprotkb/P56199) | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | [BioLegend](https://www.biolegend.com/) | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD49a_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P05362](https://www.uniprot.org/uniprotkb/P05362) | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | [BioLegend](https://www.biolegend.com/) | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD54_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P05362](https://www.uniprot.org/uniprotkb/P05362) | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | [BioLegend](https://www.biolegend.com/) | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD54_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P05362](https://www.uniprot.org/uniprotkb/P05362) | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | [BioLegend](https://www.biolegend.com/) | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD54_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P05362](https://www.uniprot.org/uniprotkb/P05362) | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | [BioLegend](https://www.biolegend.com/) | 353125 | AF700 | AB_2810560 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD54_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P13591](https://www.uniprot.org/uniprotkb/P13591) | Primary Antibody | CD56 | Human | Mouse | IgG2a | MEM-188 | [BioLegend](https://www.biolegend.com/) | 304611 | AF488 | AB_493179 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD56_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P13591](https://www.uniprot.org/uniprotkb/P13591) | Primary Antibody | CD56 | Human | Mouse | IgG2a | MEM-188 | [BioLegend](https://www.biolegend.com/) | 304605 | PE | AB_314447 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Ileum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD56_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P05362](https://www.uniprot.org/uniprotkb/P05362) | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | [BioLegend](https://www.biolegend.com/) | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD54_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P05362](https://www.uniprot.org/uniprotkb/P05362) | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | [BioLegend](https://www.biolegend.com/) | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD54_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P13591](https://www.uniprot.org/uniprotkb/P13591) | Primary Antibody | CD56 | Human | Mouse | IgG2a | MEM-188 | [BioLegend](https://www.biolegend.com/) | 304603 | FITC | AB_314445 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD56_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P13591](https://www.uniprot.org/uniprotkb/P13591) | Primary Antibody | CD56 | Human | Mouse | IgG2a | MEM-188 | [BioLegend](https://www.biolegend.com/) | 304605 | PE | AB_314447 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Ileum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD56_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P13591](https://www.uniprot.org/uniprotkb/P13591) | Primary Antibody | CD56 | Human | Mouse | IgG2a | MEM-188 | [BioLegend](https://www.biolegend.com/) | 304611 | AF488 | AB_493179 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD56_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P13591](https://www.uniprot.org/uniprotkb/P13591) | Primary Antibody | CD56 | Human | Mouse | IgG1 | 123C3.D5 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-33132AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/CD56_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P13591](https://www.uniprot.org/uniprotkb/P13591) | Primary Antibody | CD56 | Human | Mouse | IgG1 | 123C3 | [Bio-Rad](https://www.bio-rad.com/) | MCA2693A488T | AF488 | AB_1102209 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD56_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P13591](https://www.uniprot.org/uniprotkb/P13591) | Primary Antibody | CD56 | Human | Mouse | IgG1 | 123C3.D5 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-33132AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD56_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P13591](https://www.uniprot.org/uniprotkb/P13591) | Primary Antibody | CD56 | Human | Mouse | IgG1 | 123C3.D5 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-33132AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/CD56_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [F7DRX3](https://www.uniprot.org/uniprotkb/F7DRX3) | Primary Antibody | CD57 | Human | Mouse | IgM | HNK-1 | [BioLegend](https://www.biolegend.com/) | 359602 | Unconjugated | AB_2562403 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD57_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [F7DRX3](https://www.uniprot.org/uniprotkb/F7DRX3) | Primary Antibody | CD57 | Human | Mouse | IgM | HNK-1 | [BioLegend](https://www.biolegend.com/) | 359602 | Unconjugated | AB_2562403 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD57_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P05106](https://www.uniprot.org/uniprotkb/P05106) | Primary Antibody | CD61 | Human | Mouse | IgG1 | Y2/51 | [Miltenyi Biotec](https://www.miltenyibiotec.com/) | 130-098-683 | FITC | AB_2660493 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0002-3882-457X](supporting_material/CD61_FITC/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | | [P26151](https://www.uniprot.org/uniprotkb/P26151) | Primary Antibody | CD64 | Mouse | Mouse | IgG1 | X54-5/7.1 | [BioLegend](https://www.biolegend.com/) | 139322 | AF647 | AB_2566561 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD64_AF647/0000-0003-4379-8967.md), [0000-0002-1461-0999](supporting_material/CD64_AF647/0000-0002-1461-0999.md) | NA | 0000-0003-4379-8967 | | [P26151](https://www.uniprot.org/uniprotkb/P26151) | Primary Antibody | CD64 | Mouse | Mouse | IgG1 | X54-5/7.1 | [BioLegend](https://www.biolegend.com/) | 139304 | PE | AB_10612740 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/CD64_PE/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | | [P13688](https://www.uniprot.org/uniprotkb/P13688) | Primary Antibody | CD66abce | Rhesus Macaque | Mouse | IgG2b | Tet2 | [Miltenyi Biotec](https://www.miltenyibiotec.com/) | 130-116-522 | FITC | AB_2727588 | Stock | Opal-plex | 10% Formalin for 7 Days | Lung | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | [0000-0003-0666-4804](supporting_material/CD66abce_FITC/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | -| [P31997](https://www.uniprot.org/uniprotkb/P31997) | Primary Antibody | CD66b | Human | Mouse | IgM | G10F5 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1075136 | iF594 | AB_2892744 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD66b_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P31997](https://www.uniprot.org/uniprotkb/P31997) | Primary Antibody | CD66b | Human | Mouse | IgM | G10F5 | [BioLegend](https://www.biolegend.com/) | 305110 | AF647 | AB_2563171 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD66b_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD68_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P31997](https://www.uniprot.org/uniprotkb/P31997) | Primary Antibody | CD66b | Human | Mouse | IgM | G10F5 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1075136 | iF594 | AB_2892744 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD66b_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD68_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-20060 AF647 | AF647 | AB_3073741 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD68_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/CD68_iF594/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | +| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD68_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-20060 AF647 | AF647 | AB_3073741 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD68_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [A0A8C0Q9Z4](https://www.uniprot.org/uniprotkb/A0A8C0Q9Z4) | Primary Antibody | CD68 | Canine | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/CD68_iF594/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | | [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD68_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD68_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P31996](https://www.uniprot.org/uniprotkb/P31996) | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | [BioLegend](https://www.biolegend.com/) | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-1461-0999](supporting_material/CD68_AF647/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | -| [P31996](https://www.uniprot.org/uniprotkb/P31996) | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | [BioLegend](https://www.biolegend.com/) | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-1461-0999](supporting_material/CD68_AF647/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | -| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-20060 AF647 | AF647 | AB_3073741 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD68_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P31996](https://www.uniprot.org/uniprotkb/P31996) | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | [BioLegend](https://www.biolegend.com/) | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Uterus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2634-6838](supporting_material/CD68_AF647/0000-0003-2634-6838.md) | NA | 0000-0003-2634-6838 | +| [P31996](https://www.uniprot.org/uniprotkb/P31996) | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | [BioLegend](https://www.biolegend.com/) | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0001-5813-2581](supporting_material/CD68_AF647/0000-0001-5813-2581.md) | NA | 0000-0001-5813-2581 | +| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD68_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD68_iF594/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD68_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/CD68_iF594/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | -| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD68_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P31996](https://www.uniprot.org/uniprotkb/P31996) | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | [BioLegend](https://www.biolegend.com/) | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-1461-0999](supporting_material/CD68_AF647/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | | [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-20060 AF647 | AF647 | AB_3073741 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD68_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-20060 AF647 | AF647 | AB_3073741 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD68_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-20060 AF647 | AF647 | AB_3073741 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD68_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P31996](https://www.uniprot.org/uniprotkb/P31996) | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | [BioLegend](https://www.biolegend.com/) | 137011 | AF488 | AB_2074847 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD68_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P31996](https://www.uniprot.org/uniprotkb/P31996) | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | [BioLegend](https://www.biolegend.com/) | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD68_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P31996](https://www.uniprot.org/uniprotkb/P31996) | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | [BioLegend](https://www.biolegend.com/) | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD68_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD68_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P31996](https://www.uniprot.org/uniprotkb/P31996) | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | [BioLegend](https://www.biolegend.com/) | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Uterus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2634-6838](supporting_material/CD68_AF647/0000-0003-2634-6838.md) | NA | 0000-0003-2634-6838 | -| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD68_iF594/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P31996](https://www.uniprot.org/uniprotkb/P31996) | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | [BioLegend](https://www.biolegend.com/) | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD68_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P31996](https://www.uniprot.org/uniprotkb/P31996) | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | [BioLegend](https://www.biolegend.com/) | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD68_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD68_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [A0A8C0Q9Z4](https://www.uniprot.org/uniprotkb/A0A8C0Q9Z4) | Primary Antibody | CD68 | Canine | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/CD68_iF594/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | -| [P31996](https://www.uniprot.org/uniprotkb/P31996) | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | [BioLegend](https://www.biolegend.com/) | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0001-5813-2581](supporting_material/CD68_AF647/0000-0001-5813-2581.md) | NA | 0000-0001-5813-2581 | -| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD68_iF594/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P31996](https://www.uniprot.org/uniprotkb/P31996) | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | [BioLegend](https://www.biolegend.com/) | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD68_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P31996](https://www.uniprot.org/uniprotkb/P31996) | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | [BioLegend](https://www.biolegend.com/) | 137011 | AF488 | AB_2074847 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD68_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q07108](https://www.uniprot.org/uniprotkb/Q07108) | Primary Antibody | CD69 | Human | Mouse | IgG1 | FN50 | [BioLegend](https://www.biolegend.com/) | 310918 | AF647 | AB_528871 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD69_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD68_iF594/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P34810](https://www.uniprot.org/uniprotkb/P34810) | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1064135 | iF594 | AB_2892745 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD68_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q07108](https://www.uniprot.org/uniprotkb/Q07108) | Primary Antibody | CD69 | Human | Mouse | IgG1 | FN50 | [BioLegend](https://www.biolegend.com/) | 310918 | AF647 | AB_528871 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD69_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q07108](https://www.uniprot.org/uniprotkb/Q07108) | Primary Antibody | CD69 | Human | Mouse | IgG2a | 15B5G2 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-25236AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/CD69_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q07108](https://www.uniprot.org/uniprotkb/Q07108) | Primary Antibody | CD69 | Human | Mouse | IgG1 | FN50 | [BioLegend](https://www.biolegend.com/) | 310906 | PE | AB_314841 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-3218-4479](supporting_material/CD69_PE/0000-0002-3218-4479.md) | NA | 0000-0002-3218-4479 | +| [Q07108](https://www.uniprot.org/uniprotkb/Q07108) | Primary Antibody | CD69 | Human | Mouse | IgG1 | FN50 | [BioLegend](https://www.biolegend.com/) | 310918 | AF647 | AB_528871 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD69_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q61503](https://www.uniprot.org/uniprotkb/Q61503) | Primary Antibody | CD73 | Mouse | Rat | IgG1 | TY/11.8 | [BioLegend](https://www.biolegend.com/) | 127230 | AF700 | AB_2800625 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | [0000-0003-2634-6838](supporting_material/CD73_AF700/0000-0003-2634-6838.md) | NA | 0000-0003-2634-6838 | -| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | RPA-T8 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0088-24 | AF532 | AB_11220473 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD8_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | [BioLegend](https://www.biolegend.com/) | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Cervix | Cervical Cancer | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7586-9996](supporting_material/CD8_AF647/0000-0002-7586-9996.md) | NA | 0000-0002-7586-9996 | +| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Skin | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7586-9996](supporting_material/CD8_AF647/0000-0002-7586-9996.md) | NA | 0000-0002-7586-9996 | +| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Heart | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7586-9996](supporting_material/CD8_AF647/0000-0002-7586-9996.md) | NA | 0000-0002-7586-9996 | +| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4184-3867](supporting_material/CD8_AF647/0000-0003-4184-3867.md) | NA | 0000-0003-4184-3867 | +| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD8_AF647/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372910 | Spark YG 570 | AB_2904405 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD8_Spark_YG_570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BioLegend](https://www.biolegend.com/) | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD8_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BioLegend](https://www.biolegend.com/) | 100724 | AF647 | AB_389326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | CD8 | Canine | Rat | IgG1 | YCATE55.9 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA5-28430 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/CD8_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | +| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | RPA-T8 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-0088-24 | AF532 | AB_11220473 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD8_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | RPA-T8 | [BD Biosciences](https://www.bdbiosciences.com/) | 557945 | AF700 | AB_396953 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD8_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | [AAT Bioquest](https://www.aatbio.com/) | 100810C0 | iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | [BioLegend](https://www.biolegend.com/) | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 14-0085-82 | Unconjugated | AB_11150240 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD8_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD8_AF647/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BioLegend](https://www.biolegend.com/) | 100724 | AF647 | AB_389326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | [BioLegend](https://www.biolegend.com/) | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | [BioLegend](https://www.biolegend.com/) | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | [BioLegend](https://www.biolegend.com/) | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Cervix | Cervical Cancer | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7586-9996](supporting_material/CD8_AF647/0000-0002-7586-9996.md) | NA | 0000-0002-7586-9996 | -| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Skin | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7586-9996](supporting_material/CD8_AF647/0000-0002-7586-9996.md) | NA | 0000-0002-7586-9996 | | [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/CD8_AF647/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | [BioLegend](https://www.biolegend.com/) | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | [BioLegend](https://www.biolegend.com/) | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | [BioLegend](https://www.biolegend.com/) | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | [BioLegend](https://www.biolegend.com/) | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | [BioLegend](https://www.biolegend.com/) | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF647/0000-0003-4379-8967.md), [0009-0006-9784-2694](supporting_material/CD8_AF647/0009-0006-9784-2694.md) | NA | 0000-0003-4379-8967 | +| [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BioLegend](https://www.biolegend.com/) | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-1431-1398](supporting_material/CD8_BV421/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | | [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/CD8_AF647/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | +| [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BioLegend](https://www.biolegend.com/) | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD8_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BioLegend](https://www.biolegend.com/) | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD8_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Rabbit | IgG | EPR10640 | [Cell IDx](https://cellidx.com/) | HI06B-005 | UT015 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/CD8_UT015/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF647/0000-0003-4379-8967.md), [0009-0006-9784-2694](supporting_material/CD8_AF647/0009-0006-9784-2694.md) | NA | 0000-0003-4379-8967 | -| [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BioLegend](https://www.biolegend.com/) | 100752 | BV510 | AB_2563057 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD8_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BioLegend](https://www.biolegend.com/) | 100723 | AF488 | AB_389304 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372910 | Spark YG 570 | AB_2904405 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CD8_Spark_YG_570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BD Biosciences](https://www.bdbiosciences.com/) | 553032 | PE | AB_394571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Heart | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7586-9996](supporting_material/CD8_AF647/0000-0002-7586-9996.md) | NA | 0000-0002-7586-9996 | -| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | [BioLegend](https://www.biolegend.com/) | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BioLegend](https://www.biolegend.com/) | 100758 | AF594 | AB_2563237 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BioLegend](https://www.biolegend.com/) | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD8_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | [BioLegend](https://www.biolegend.com/) | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BD Biosciences](https://www.bdbiosciences.com/) | 553032 | PE | AB_394571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BioLegend](https://www.biolegend.com/) | 100723 | AF488 | AB_389304 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BioLegend](https://www.biolegend.com/) | 100752 | BV510 | AB_2563057 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD8_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BioLegend](https://www.biolegend.com/) | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD8_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BioLegend](https://www.biolegend.com/) | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-1431-1398](supporting_material/CD8_BV421/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | -| [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BioLegend](https://www.biolegend.com/) | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD8_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD8_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | [BioLegend](https://www.biolegend.com/) | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4184-3867](supporting_material/CD8_AF647/0000-0003-4184-3867.md) | NA | 0000-0003-4184-3867 | -| [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | [BioLegend](https://www.biolegend.com/) | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/CD8_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P33681](https://www.uniprot.org/uniprotkb/P33681) | Primary Antibody | CD80 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF140 | Unconjugated | AB_354778 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD80_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P33681](https://www.uniprot.org/uniprotkb/P33681) | Primary Antibody | CD80 | Human | Mouse | IgG2b | 62N3G8 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-25255AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-3882-457X](supporting_material/CD80_AF488/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | | [P33681](https://www.uniprot.org/uniprotkb/P33681) | Primary Antibody | CD80 | Human | Mouse | IgG1 | 2D10 | [BioLegend](https://www.biolegend.com/) | 305215 | AF647 | AB_528874 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CD80_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | @@ -579,39 +585,39 @@ Additional information on fluorescent probes tested by the IBEX Imaging Communit | [P01732](https://www.uniprot.org/uniprotkb/P01732) | Primary Antibody | CD8a | Mouse | Mouse | IgG1 | HIT8a | [BioLegend](https://www.biolegend.com/) | 300908 | PE | AB_314112 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/CD8a_PE/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | | [P01731](https://www.uniprot.org/uniprotkb/P01731) | Primary Antibody | CD8a | Mouse | Rat | IgG2a | 53-6.7 | [R&D Systems](https://www.rndsystems.com/) | FAB116S-100UG | AF750 | NA | Stock | Ce3D | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | NA | No | [0000-0002-0835-911X](supporting_material/CD8a_AF750/0000-0002-0835-911X.md) | NA | 0000-0002-0835-911X | | [P01831](https://www.uniprot.org/uniprotkb/P01831) | Primary Antibody | CD90.1 | Mouse | Mouse | IgG1 | OX-7 | [BioLegend](https://www.biolegend.com/) | 202508 | AF647 | AB_492884 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-1461-0999](supporting_material/CD90.1_AF647/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | +| [Q13241](https://www.uniprot.org/uniprotkb/Q13241) | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | [BioLegend](https://www.biolegend.com/) | 305512 | AF647 | AB_2249691 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD94_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q13241](https://www.uniprot.org/uniprotkb/Q13241) | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | [BioLegend](https://www.biolegend.com/) | 305506 | PE | AB_314536 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD94_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q13241](https://www.uniprot.org/uniprotkb/Q13241) | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | [BioLegend](https://www.biolegend.com/) | 305506 | PE | AB_314536 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD94_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q13241](https://www.uniprot.org/uniprotkb/Q13241) | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | [BioLegend](https://www.biolegend.com/) | 305504 | FITC | AB_314534 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD94_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q13241](https://www.uniprot.org/uniprotkb/Q13241) | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | [BioLegend](https://www.biolegend.com/) | 305512 | AF647 | AB_2249691 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CD94_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q13241](https://www.uniprot.org/uniprotkb/Q13241) | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | [BioLegend](https://www.biolegend.com/) | 305506 | AF488 | AB_314536 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/CD94_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q13241](https://www.uniprot.org/uniprotkb/Q13241) | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | [BioLegend](https://www.biolegend.com/) | 305506 | PE | AB_314536 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CD94_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9NXV6](https://www.uniprot.org/uniprotkb/Q9NXV6) | Primary Antibody | CDKN2a | Human | Rat | IgG2b | 5-C3-1 | [Novus Biologicals](https://www.novusbio.com/) | NB200-174AF647 | AF647 | AB_10002046 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CDKN2a_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9NXV6](https://www.uniprot.org/uniprotkb/Q9NXV6) | Primary Antibody | CDKN2a | Human | Mouse | IgG2a | ARF 4C6/4 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-53392 | Unconjugated | AB_630056 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/CDKN2a_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P13569](https://www.uniprot.org/uniprotkb/P13569) | Primary Antibody | CFTR | Human | Mouse | IgG1 | M3A7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA5-11768 | Unconjugated | AB_10983660 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](supporting_material/CFTR_Unconjugated/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | -| [P13569](https://www.uniprot.org/uniprotkb/P13569) | Primary Antibody | CFTR | Human | Mouse | IgM | CF3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA1-935 | Unconjugated | AB_2081230 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | [0000-0002-2950-2683](supporting_material/CFTR_Unconjugated/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | | [P13569](https://www.uniprot.org/uniprotkb/P13569) | Primary Antibody | CFTR | Human | Mouse | IgM | CF3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA1-935 | Unconjugated | AB_2081230 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](supporting_material/CFTR_Unconjugated/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | +| [P13569](https://www.uniprot.org/uniprotkb/P13569) | Primary Antibody | CFTR | Human | Mouse | IgM | CF3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA1-935 | Unconjugated | AB_2081230 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | [0000-0002-2950-2683](supporting_material/CFTR_Unconjugated/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | +| [P13569](https://www.uniprot.org/uniprotkb/P13569) | Primary Antibody | CFTR | Human | Mouse | IgG1 | M3A7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MA5-11768 | Unconjugated | AB_10983660 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](supporting_material/CFTR_Unconjugated/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | | NA | Secondary Antibody | Chicken IgY (H&L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | SA1-72000 | FITC | AB_923386 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/Chicken_IgY__H_L__FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Chicken IgY (H&L) | Zebrafish | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11039 | AF488 | AB_2534096 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Chicken_IgY__H_L__AF488/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | NA | Secondary Antibody | Chicken IgY (H&L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-32932 | AF555 | AB_2762844 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0002-5187-810X](supporting_material/Chicken_IgY__H_L__AF555/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | +| NA | Secondary Antibody | Chicken IgY (H&L) | Zebrafish | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11039 | AF488 | AB_2534096 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Chicken_IgY__H_L__AF488/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [P10645](https://www.uniprot.org/uniprotkb/P10645) | Primary Antibody | Chromogranin A | Human | Mouse | IgG1 | LK2H10 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-34672AF532 | AF532 | AB_2892553 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-3882-457X](supporting_material/Chromogranin_A_AF532/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | | [P58304](https://www.uniprot.org/uniprotkb/P58304) | Primary Antibody | Chx10 | Mouse | Mouse | IgG2a | E-12 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-365519 AF546 | AF546 | AB_10842442 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | [0000-0003-2088-8310](supporting_material/Chx10_AF546/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [P70194](https://www.uniprot.org/uniprotkb/P70194) | Primary Antibody | CLEC4F | Mouse | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2784 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | [0000-0003-0666-4804](supporting_material/CLEC4F_Unconjugated/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | | [Q6UXN8](https://www.uniprot.org/uniprotkb/Q6UXN8) | Primary Antibody | CLEC9a | Human | Mouse | IgG2a | 8F9 | [BioLegend](https://www.biolegend.com/) | 353802 (Unconjugated) | AF488 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CLEC9a_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9BWP8](https://www.uniprot.org/uniprotkb/Q9BWP8) | Primary Antibody | COLEC11 | Human | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 15269-1-AP | Unconjugated | AB_10732940 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-3882-457X](supporting_material/COLEC11_Unconjugated/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | -| [P02463](https://www.uniprot.org/uniprotkb/P02463) | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Collagen_IV_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P02463](https://www.uniprot.org/uniprotkb/P02463) | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Collagen_IV_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P02463](https://www.uniprot.org/uniprotkb/P02463) | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Collagen_IV_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P02462](https://www.uniprot.org/uniprotkb/P02462) | Primary Antibody | Collagen IV | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab6586 | Unconjugated | AB_305584 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Collagen_IV_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P02463](https://www.uniprot.org/uniprotkb/P02463) | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Collagen_IV_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P02462](https://www.uniprot.org/uniprotkb/P02462) | Primary Antibody | Collagen IV | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab6586 | Unconjugated | AB_305584 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Collagen_IV_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P02463](https://www.uniprot.org/uniprotkb/P02463) | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Collagen_IV_Unconjugated/0000-0003-4379-8967.md), [0000-0003-1118-7432](supporting_material/Collagen_IV_Unconjugated/0000-0003-1118-7432.md) | NA | 0000-0003-4379-8967 | | [P02462](https://www.uniprot.org/uniprotkb/P02462) | Primary Antibody | Collagen IV | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab6586 | Unconjugated | AB_305584 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Collagen_IV_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P02462](https://www.uniprot.org/uniprotkb/P02462) | Primary Antibody | Collagen IV | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab6586 | Unconjugated | AB_305584 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Collagen_IV_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P02463](https://www.uniprot.org/uniprotkb/P02463) | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Collagen_IV_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P02463](https://www.uniprot.org/uniprotkb/P02463) | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Collagen_IV_Unconjugated/0000-0003-4379-8967.md), [0000-0003-1118-7432](supporting_material/Collagen_IV_Unconjugated/0000-0003-1118-7432.md) | NA | 0000-0003-4379-8967 | | [Q6PBM9](https://www.uniprot.org/uniprotkb/Q6PBM9) | Primary Antibody | Cone Arrestin | Zebrafish | Rabbit | IgG | Polyclonal | [MilliporeSigma](https://www.sigmaaldrich.com/) | AB15282 | Unconjugated | AB_1163387 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Cone_Arrestin_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [Q9EQP6](https://www.uniprot.org/uniprotkb/Q9EQP6) | Primary Antibody | Cone Arrestin | Mouse | Rabbit | IgG | Polyclonal | [MilliporeSigma](https://www.sigmaaldrich.com/) | AB15282 | Unconjugated | AB_1163387 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | [0000-0003-2088-8310](supporting_material/Cone_Arrestin_Unconjugated/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [Q6FHL8](https://www.uniprot.org/uniprotkb/Q6FHL8) | Primary Antibody | CTGF | Human | Rabbit | IgG | OT11F4 | [FabGennix](https://fabgennix.com/) | CTGF-FITC | FITC | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/CTGF_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16410](https://www.uniprot.org/uniprotkb/P16410) | Primary Antibody | CTLA4 | Human | Rabbit | IgG | CAL49 | [Abcam](https://www.abcam.com/) | ab270127 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CTLA4_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P16410](https://www.uniprot.org/uniprotkb/P16410) | Primary Antibody | CTLA4 | Human | Rabbit | IgG | CAL49 | [Abcam](https://www.abcam.com/) | ab270127 | AF647 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CTLA4_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16410](https://www.uniprot.org/uniprotkb/P16410) | Primary Antibody | CTLA4 | Human | Rabbit | IgG | CAL49 | [Abcam](https://www.abcam.com/) | ab270127 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/CTLA4_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P16410](https://www.uniprot.org/uniprotkb/P16410) | Primary Antibody | CTLA4 | Human | Rabbit | IgG | CAL49 | [Abcam](https://www.abcam.com/) | ab270127 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0009-0006-9784-2694](supporting_material/CTLA4_AF647/0009-0006-9784-2694.md) | NA | 0009-0006-9784-2694 | -| [P63158](https://www.uniprot.org/uniprotkb/P63158) | Primary Antibody | CXCL12 | Mouse | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | AB25117 | NA | AB_2088164 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](supporting_material/CXCL12_NA/0000-0002-6944-6997.md) | NA | 0000-0002-6944-6997 | | [Q6ICW0](https://www.uniprot.org/uniprotkb/Q6ICW0) | Primary Antibody | CXCL12 | Human | Mouse | IgG1 | 79018 | [R&D Systems](https://www.rndsystems.com/) | MAB350-500 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CXCL12_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P63158](https://www.uniprot.org/uniprotkb/P63158) | Primary Antibody | CXCL12 | Mouse | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | AB25117 | NA | AB_2088164 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](supporting_material/CXCL12_NA/0000-0002-6944-6997.md) | NA | 0000-0002-6944-6997 | | [Q6ICW0](https://www.uniprot.org/uniprotkb/Q6ICW0) | Primary Antibody | CXCL12 | Human | Mouse | IgG1 | 79018 | [R&D Systems](https://www.rndsystems.com/) | MAB350 | Unconjugated | AB_2088149 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/CXCL12_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q53X90](https://www.uniprot.org/uniprotkb/Q53X90) | Primary Antibody | CXCL13 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF801 (Unconjugated) | AF532 | AB_2892755 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/CXCL13_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q53X90](https://www.uniprot.org/uniprotkb/Q53X90) | Primary Antibody | CXCL13 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF801 | Unconjugated | AB_2892755 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/CXCL13_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | @@ -623,268 +629,268 @@ Additional information on fluorescent probes tested by the IBEX Imaging Communit | [Q9EQ16](https://www.uniprot.org/uniprotkb/Q9EQ16) | Primary Antibody | CXCR6 | Mouse | Rat | IgG2b | FAB2145R | [Novus Biologicals](https://www.novusbio.com/) | 221002 | AF647 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/CXCR6_AF647/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | | [P56485](https://www.uniprot.org/uniprotkb/P56485) | Primary Antibody | CXCR7 | Mouse | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NBP2-24779G | DL488 | NA | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](supporting_material/CXCR7_DL488/0000-0002-6944-6997.md) | NA | 0000-0002-6944-6997 | | NA | Primary Antibody | Cytokeratin | Mouse | Mouse | IgG1 | C-11 | [BioLegend](https://www.biolegend.com/) | 628604 | AF647 | AB_2563652 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Cytokeratin_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | Cytokeratin (Pan) | Canine | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9003-82 | AF488 | AB_2574301 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0001-5088-7808](supporting_material/Cytokeratin__Pan__AF488/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | | NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/Cytokeratin__Pan__AF488/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/Cytokeratin__Pan__AF488/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | -| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-33200AF750 | AF750 | AB_2868569 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-5187-810X](supporting_material/Cytokeratin__Pan__AF750/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | -| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 42-9003-82 | eF615 | AB_10804663 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Cytokeratin__Pan__eF615/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9003-82 | AF488 | AB_2574301 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Cytokeratin__Pan__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-9003-80 | eF570 | AB_11217482 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Cytokeratin__Pan__eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-9003-82 | eF660 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-3697-5726](supporting_material/Cytokeratin__Pan__eF660/0000-0003-3697-5726.md) | NA | 0000-0003-3697-5726 | | NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-9003-82 | eF660 | AB_2574301 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Cytokeratin__Pan__eF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-33200AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/Cytokeratin__Pan__AF700/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9003-82 | AF488 | AB_2574301 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Cytokeratin__Pan__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/Cytokeratin__Pan__AF488/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | | NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/Cytokeratin__Pan__AF488/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Cytokeratin__Pan__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-9003-82 | eF660 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-3697-5726](supporting_material/Cytokeratin__Pan__eF660/0000-0003-3697-5726.md) | NA | 0000-0003-3697-5726 | -| NA | Primary Antibody | Cytokeratin (Pan) | Canine | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9003-82 | AF488 | AB_2574301 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0001-5088-7808](supporting_material/Cytokeratin__Pan__AF488/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | | NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [BioLegend](https://www.biolegend.com/) | 914204 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-3882-457X](supporting_material/Cytokeratin__Pan__AF532/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | +| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Cytokeratin__Pan__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-9003-80 | eF570 | AB_11217482 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Cytokeratin__Pan__eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-33200AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/Cytokeratin__Pan__AF700/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 42-9003-82 | eF615 | AB_10804663 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Cytokeratin__Pan__eF615/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-33200AF750 | AF750 | AB_2868569 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-5187-810X](supporting_material/Cytokeratin__Pan__AF750/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | | [P13645](https://www.uniprot.org/uniprotkb/P13645) | Primary Antibody | Cytokeratin 10 | Human | Mouse | IgG1 | DE-K10 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-54402AF488 | AF488 | AB_2892746 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0002-5187-810X](supporting_material/Cytokeratin_10_AF488/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | | [P02533](https://www.uniprot.org/uniprotkb/P02533) | Primary Antibody | Cytokeratin 14 | Human | Chicken | IgY | Poly9060 | [BioLegend](https://www.biolegend.com/) | 906004 (Unconjugated) | AF647 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/Cytokeratin_14_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P02533](https://www.uniprot.org/uniprotkb/P02533) | Primary Antibody | Cytokeratin 14 | Human | Chicken | IgY | Poly9060 | [BioLegend](https://www.biolegend.com/) | 906004 | Unconjugated | AB_2616962 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-5187-810X](supporting_material/Cytokeratin_14_Unconjugated/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | | [P02533](https://www.uniprot.org/uniprotkb/P02533) | Primary Antibody | Cytokeratin 14 | Human | Chicken | IgY | Poly9060 | [BioLegend](https://www.biolegend.com/) | 906004 | Unconjugated | AB_2616962 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Cytokeratin_14_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P02533](https://www.uniprot.org/uniprotkb/P02533) | Primary Antibody | Cytokeratin 14 | Human | Chicken | IgY | Poly9060 | [BioLegend](https://www.biolegend.com/) | 906004 | Unconjugated | AB_2616962 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-5187-810X](supporting_material/Cytokeratin_14_Unconjugated/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | | [P05784](https://www.uniprot.org/uniprotkb/P05784) | Primary Antibody | Cytokeratin 18 | Mouse | Rabbit | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | PA5-14263 | Unconjugated | AB_2133178 | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | [0000-0003-0666-4804](supporting_material/Cytokeratin_18_Unconjugated/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | | [P08729](https://www.uniprot.org/uniprotkb/P08729) | Primary Antibody | Cytokeratin 7 | Human | Rat | IgG2a | W16155A | [BioLegend](https://www.biolegend.com/) | 601606 | AF488 | AB_2728458 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-3218-4479](supporting_material/Cytokeratin_7_AF488/0000-0002-3218-4479.md) | NA | 0000-0002-3218-4479 | | NA | Nuclear Dye | DAPI | Zebrafish | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | D1306 | NA | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/DAPI_NA/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| [Q9NNX6](https://www.uniprot.org/uniprotkb/Q9NNX6) | Primary Antibody | DC-SIGN | Human | Mouse | IgG2a | 9E9A8 | [BioLegend](https://www.biolegend.com/) | 330112 | AF647 | AB_1186092 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/DC-SIGN_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9NNX6](https://www.uniprot.org/uniprotkb/Q9NNX6) | Primary Antibody | DC-SIGN | Human | Rat | IgG2a | h209 | [LSBio](https://www.lsbio.com/) | LS-B3782 | Unconjugated | AB_10689801 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/DC-SIGN_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9NNX6](https://www.uniprot.org/uniprotkb/Q9NNX6) | Primary Antibody | DC-SIGN | Human | Mouse | IgG2a | DC28 | [R&D Systems](https://www.rndsystems.com/) | MAB16211-SP | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/DC-SIGN_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9NNX6](https://www.uniprot.org/uniprotkb/Q9NNX6) | Primary Antibody | DC-SIGN | Human | Rat | IgG2a | h209 | [LSBio](https://www.lsbio.com/) | LS-B3782 | Unconjugated | AB_10689801 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/DC-SIGN_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9NNX6](https://www.uniprot.org/uniprotkb/Q9NNX6) | Primary Antibody | DC-SIGN | Human | Mouse | IgG2b | 102E11.06 | [Eurobio Scientific (Dendritics)](https://www.eurobio-scientific.com/) | DDX0202A647-100 | AF647 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/DC-SIGN_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9NNX6](https://www.uniprot.org/uniprotkb/Q9NNX6) | Primary Antibody | DC-SIGN | Human | Mouse | IgG2a | 9E9A8 | [BioLegend](https://www.biolegend.com/) | 330112 | AF647 | AB_1186092 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/DC-SIGN_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9NNX6](https://www.uniprot.org/uniprotkb/Q9NNX6) | Primary Antibody | DC-SIGN | Human | Mouse | IgG2a | DC28 | [R&D Systems](https://www.rndsystems.com/) | MAB16211-SP | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/DC-SIGN_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9NNX6](https://www.uniprot.org/uniprotkb/Q9NNX6) | Primary Antibody | DC-SIGN | Human | Rat | IgG2a | h209 | [LSBio](https://www.lsbio.com/) | LS-B3782 | Unconjugated | AB_10689801 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/DC-SIGN_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9NNX6](https://www.uniprot.org/uniprotkb/Q9NNX6) | Primary Antibody | DC-SIGN | Human | Mouse | IgG2b | C209/1781 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-54351AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/DC-SIGN_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9NNX6](https://www.uniprot.org/uniprotkb/Q9NNX6) | Primary Antibody | DC-SIGN | Human | Mouse | IgG2a | 9E9A8 | [BioLegend](https://www.biolegend.com/) | 330112 | AF647 | AB_1186092 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/DC-SIGN_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9NNX6](https://www.uniprot.org/uniprotkb/Q9NNX6) | Primary Antibody | DC-SIGN | Human | Mouse | IgG2b | 102E11.06 | [Eurobio Scientific (Dendritics)](https://www.eurobio-scientific.com/) | DDX0202A647-100 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/DC-SIGN_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [O15075](https://www.uniprot.org/uniprotkb/O15075) | Primary Antibody | DCAMKL1 | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab31704 | Unconjugated | AB_873537 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/DCAMKL1_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9NNX6](https://www.uniprot.org/uniprotkb/Q9NNX6) | Primary Antibody | DC-SIGN | Human | Mouse | IgG2a | 9E9A8 | [BioLegend](https://www.biolegend.com/) | 330112 | AF647 | AB_1186092 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/DC-SIGN_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9JLM8](https://www.uniprot.org/uniprotkb/Q9JLM8) | Primary Antibody | DCAMKL1 | Mouse | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab37994 | Unconjugated | AB_873538 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/DCAMKL1_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [O15075](https://www.uniprot.org/uniprotkb/O15075) | Primary Antibody | DCAMKL1 | Human | Rabbit | IgG | EPR6085 | [Abcam](https://www.abcam.com/) | ab187184 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-3882-457X](supporting_material/DCAMKL1_Unconjugated/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | +| [O15075](https://www.uniprot.org/uniprotkb/O15075) | Primary Antibody | DCAMKL1 | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab31704 | Unconjugated | AB_873537 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/DCAMKL1_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q16832](https://www.uniprot.org/uniprotkb/Q16832) | Primary Antibody | DDR2 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2538 | Unconjugated | AB_416613 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/DDR2_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q60767](https://www.uniprot.org/uniprotkb/Q60767) | Primary Antibody | DEC205 | Mouse | Rat | IgG2a | NLDC-145 | [BioLegend](https://www.biolegend.com/) | 138204 | AF647 | AB_2137655 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/DEC205_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q60767](https://www.uniprot.org/uniprotkb/Q60767) | Primary Antibody | DEC205 | Mouse | Rat | IgG2a | NLDC-145 | [BioLegend](https://www.biolegend.com/) | 138204 | AF647 | AB_2137655 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/DEC205_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P17661](https://www.uniprot.org/uniprotkb/P17661) | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | [Abcam](https://www.abcam.com/) | ab185033 | AF488 | AB_2892748 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Desmin_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P17661](https://www.uniprot.org/uniprotkb/P17661) | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | [Abcam](https://www.abcam.com/) | ab185033 | AF488 | AB_2892748 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Desmin_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P17661](https://www.uniprot.org/uniprotkb/P17661) | Primary Antibody | Desmin | Mouse | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab15200 | Unconjugated | AB_301744 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | NA | Yes | [0000-0003-1431-1398](supporting_material/Desmin_Unconjugated/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | | [P17661](https://www.uniprot.org/uniprotkb/P17661) | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | [Abcam](https://www.abcam.com/) | ab185033 | AF488 | AB_2892748 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Desmin_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P17661](https://www.uniprot.org/uniprotkb/P17661) | Primary Antibody | Desmin | Mouse | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab15200 | Unconjugated | AB_301744 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | NA | Yes | [0000-0003-1431-1398](supporting_material/Desmin_Unconjugated/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | | [P17661](https://www.uniprot.org/uniprotkb/P17661) | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | [Abcam](https://www.abcam.com/) | ab185033 | AF488 | AB_2892748 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/Desmin_AF488/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [P17661](https://www.uniprot.org/uniprotkb/P17661) | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | [Abcam](https://www.abcam.com/) | ab185033 | AF488 | AB_2892748 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | No | [0000-0003-4379-8967](supporting_material/Desmin_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P12830](https://www.uniprot.org/uniprotkb/P12830) | Primary Antibody | E-cadherin | Human | Rat | IgG1 | DECMA-1 | [BioLegend](https://www.biolegend.com/) | 147307 | AF647 | AB_2563954 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Nasal Polyp | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0009-0004-8162-409X](supporting_material/E-cadherin_AF647/0009-0004-8162-409X.md) | NA | 0009-0004-8162-409X | | [P09803](https://www.uniprot.org/uniprotkb/P09803) | Primary Antibody | E-cadherin | Mouse | Rat | IgG1 | DECMA-1 | [BioLegend](https://www.biolegend.com/) | 147308 | AF647 | AB_2563955 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/E-cadherin_AF647/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | +| [P12830](https://www.uniprot.org/uniprotkb/P12830) | Primary Antibody | E-cadherin | Human | Rat | IgG1 | DECMA-1 | [BioLegend](https://www.biolegend.com/) | 147307 | AF647 | AB_2563954 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Nasal Polyp | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0009-0004-8162-409X](supporting_material/E-cadherin_AF647/0009-0004-8162-409X.md) | NA | 0009-0004-8162-409X | | [P32249](https://www.uniprot.org/uniprotkb/P32249) | Primary Antibody | EBI2 | Human | Rabbit | IgG | Polyclonal | [RayBiotech](https://www.raybiotech.com/) | 119-10961 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/EBI2_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P32249](https://www.uniprot.org/uniprotkb/P32249) | Primary Antibody | EBI2 | Human | Mouse | IgG2a | SA313E4 | [BioLegend](https://www.biolegend.com/) | 368903 | AF647 | AB_2566384 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/EBI2_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P32249](https://www.uniprot.org/uniprotkb/P32249) | Primary Antibody | EBI2 | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NLS46 | Unconjugated | AB_10001402 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/EBI2_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | EBOV NP | Mouse | Rabbit | NA | Polyclonal | [IBT BioServices](https://www.ibtbioservices.com/) | 0301-012 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | [0000-0003-0666-4804](supporting_material/EBOV_NP_Unconjugated/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | | NA | Primary Antibody | EBOV NP | Mouse | Rabbit | NA | Polyclonal | [IBT BioServices](https://www.ibtbioservices.com/) | 0301-012 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lymph Node | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | [0000-0003-0666-4804](supporting_material/EBOV_NP_Unconjugated/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | | [P16422](https://www.uniprot.org/uniprotkb/P16422) | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | [BioLegend](https://www.biolegend.com/) | 324228 | AF594 | AB_2563209 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/EpCAM_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q99JW5](https://www.uniprot.org/uniprotkb/Q99JW5) | Primary Antibody | EpCAM | Mouse | Rat | IgG2a | G8.8 | [BioLegend](https://www.biolegend.com/) | 118212 | AF647 | AB_1134101 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/EpCAM_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P16422](https://www.uniprot.org/uniprotkb/P16422) | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | [BioLegend](https://www.biolegend.com/) | 324210 | AF488 | AB_756084 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/EpCAM_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P16422](https://www.uniprot.org/uniprotkb/P16422) | Primary Antibody | EpCAM | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab71916 | Unconjugated | AB_1603782 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/EpCAM_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16422](https://www.uniprot.org/uniprotkb/P16422) | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | [BioLegend](https://www.biolegend.com/) | 324235 | BV510 | AB_2632934 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/EpCAM_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q99JW5](https://www.uniprot.org/uniprotkb/Q99JW5) | Primary Antibody | EpCAM | Mouse | Rat | IgG2a | G8.8 | [BioLegend](https://www.biolegend.com/) | 118222 | AF594 | AB_2563322 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/EpCAM_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16422](https://www.uniprot.org/uniprotkb/P16422) | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | [BioLegend](https://www.biolegend.com/) | 324202 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/EpCAM_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q99JW5](https://www.uniprot.org/uniprotkb/Q99JW5) | Primary Antibody | EpCAM | Mouse | Rat | IgG2a | G8.8 | [BioLegend](https://www.biolegend.com/) | 118212 | AF647 | AB_1134101 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/EpCAM_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16422](https://www.uniprot.org/uniprotkb/P16422) | Primary Antibody | EpCAM | Human | Mouse | IgG1 | 1B7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-9326-42 | eF660 | AB_10598658 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/EpCAM_eF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16422](https://www.uniprot.org/uniprotkb/P16422) | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | [BioLegend](https://www.biolegend.com/) | 324243 | AF700 | AB_2750488 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-3882-457X](supporting_material/EpCAM_AF700/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | -| [Q99JW5](https://www.uniprot.org/uniprotkb/Q99JW5) | Primary Antibody | EpCAM | Mouse | Rat | IgG2a | G8.8 | [BD Biosciences](https://www.bdbiosciences.com/) | 747748 | BV510 | AB_2872217 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | No | [0000-0003-4379-8967](supporting_material/EpCAM_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16422](https://www.uniprot.org/uniprotkb/P16422) | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | [BioLegend](https://www.biolegend.com/) | 324212 | AF647 | AB_756086 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/EpCAM_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P16422](https://www.uniprot.org/uniprotkb/P16422) | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | [BioLegend](https://www.biolegend.com/) | 324228 | AF594 | AB_2563209 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Metastatic | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/EpCAM_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 12-4801-82 | PE | AB_465923 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/F4_80_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | [BioLegend](https://www.biolegend.com/) | 123122 | AF647 | AB_893480 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/F4_80_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | [BioLegend](https://www.biolegend.com/) | 123130 | AF700 | AB_2293450 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/F4_80_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q14246](https://www.uniprot.org/uniprotkb/Q14246) | Primary Antibody | F4/80 | Mouse | Rat | IgG2b | F4/80 | [Abcam](https://www.abcam.com/) | ab204467 | AF647 | AB_2810932 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/F4_80_AF647/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | +| [P16422](https://www.uniprot.org/uniprotkb/P16422) | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | [BioLegend](https://www.biolegend.com/) | 324212 | AF647 | AB_756086 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/EpCAM_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16422](https://www.uniprot.org/uniprotkb/P16422) | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | [BioLegend](https://www.biolegend.com/) | 324228 | AF594 | AB_2563209 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Metastatic | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/EpCAM_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q99JW5](https://www.uniprot.org/uniprotkb/Q99JW5) | Primary Antibody | EpCAM | Mouse | Rat | IgG2a | G8.8 | [BioLegend](https://www.biolegend.com/) | 118222 | AF594 | AB_2563322 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/EpCAM_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16422](https://www.uniprot.org/uniprotkb/P16422) | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | [BioLegend](https://www.biolegend.com/) | 324243 | AF700 | AB_2750488 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-3882-457X](supporting_material/EpCAM_AF700/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | +| [Q99JW5](https://www.uniprot.org/uniprotkb/Q99JW5) | Primary Antibody | EpCAM | Mouse | Rat | IgG2a | G8.8 | [BD Biosciences](https://www.bdbiosciences.com/) | 747748 | BV510 | AB_2872217 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | No | [0000-0003-4379-8967](supporting_material/EpCAM_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16422](https://www.uniprot.org/uniprotkb/P16422) | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | [BioLegend](https://www.biolegend.com/) | 324202 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/EpCAM_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16422](https://www.uniprot.org/uniprotkb/P16422) | Primary Antibody | EpCAM | Human | Mouse | IgG1 | 1B7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-9326-42 | eF660 | AB_10598658 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/EpCAM_eF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P16422](https://www.uniprot.org/uniprotkb/P16422) | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | [BioLegend](https://www.biolegend.com/) | 324235 | BV510 | AB_2632934 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/EpCAM_BV510/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q14246](https://www.uniprot.org/uniprotkb/Q14246) | Primary Antibody | F4/80 | Mouse | Rat | IgG2b | F4/80 | [Abcam](https://www.abcam.com/) | ab204467 | AF647 | AB_2810932 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/F4_80_AF647/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | | NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | [BioLegend](https://www.biolegend.com/) | 123132 | BV421 | AB_11203717 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/F4_80_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q3S4B0](https://www.uniprot.org/uniprotkb/Q3S4B0) | Primary Antibody | F4/80 | Mouse | Rabbit | IgG | D2S9R | [Cell Signaling Technology](https://www.cellsignal.com/) | 70076 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | [0000-0003-0666-4804](supporting_material/F4_80_Unconjugated/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | +| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | [BioLegend](https://www.biolegend.com/) | 123130 | AF700 | AB_2293450 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/F4_80_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | [BioLegend](https://www.biolegend.com/) | 123122 | AF647 | AB_893480 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/F4_80_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 12-4801-82 | PE | AB_465923 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/F4_80_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q14246](https://www.uniprot.org/uniprotkb/Q14246) | Primary Antibody | F4/80 | Mouse | Rat | IgG2b | F4/80 | [Abcam](https://www.abcam.com/) | ab204467 | AF647 | AB_2810932 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/F4_80_AF647/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | | NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | [BioLegend](https://www.biolegend.com/) | 123132 | BV421 | AB_11203717 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/F4_80_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q3S4B0](https://www.uniprot.org/uniprotkb/Q3S4B0) | Primary Antibody | F4/80 | Mouse | Rabbit | IgG | D2S9R | [Cell Signaling Technology](https://www.cellsignal.com/) | 70076 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lymph Node | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | [0000-0003-0666-4804](supporting_material/F4_80_Unconjugated/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | -| [Q3S4B0](https://www.uniprot.org/uniprotkb/Q3S4B0) | Primary Antibody | F4/80 | Mouse | Rabbit | IgG | D2S9R | [Cell Signaling Technology](https://www.cellsignal.com/) | 70076 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | [0000-0003-0666-4804](supporting_material/F4_80_Unconjugated/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | -| NA | Blocking Reagent | Fc Block | Mouse | Rat | IgG2b | 2.4G2 | [BD Biosciences](https://www.bdbiosciences.com/) | 553141 | Unconjugated | AB_394656 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Fc_Block_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Blocking Reagent | Fc Block | Human | Human | IgG1 | Fc1 | [BD Biosciences](https://www.bdbiosciences.com/) | 564220 | Unconjugated | AB_2869554 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Fc_Block_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Blocking Reagent | Fc Block | Mouse | Rat | IgG2b | 2.4G2 | [BD Biosciences](https://www.bdbiosciences.com/) | 553141 | Unconjugated | AB_394656 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Fc_Block_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q8TBJ5](https://www.uniprot.org/uniprotkb/Q8TBJ5) | Primary Antibody | FEZF2 | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab214186 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-3882-457X](supporting_material/FEZF2_Unconjugated/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | -| [P02671](https://www.uniprot.org/uniprotkb/P02671) | Primary Antibody | Fibrinogen | Human | Sheep | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab118533 | Unconjugated | AB_10900171 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Fibrinogen_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P02671](https://www.uniprot.org/uniprotkb/P02671) | Primary Antibody | Fibrinogen | Human | Sheep | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab118533 | Unconjugated | AB_10900171 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Fibrinogen_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P02671](https://www.uniprot.org/uniprotkb/P02671) | Primary Antibody | Fibrinogen | Human | Sheep | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab118533 | Unconjugated | AB_10900171 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Fibrinogen_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q12884](https://www.uniprot.org/uniprotkb/Q12884) | Primary Antibody | Fibroblast activation protein alpha | Human | Rabbit | IgG | Polyclonal | [MilliporeSigma](https://www.sigmaaldrich.com/) | ABT11 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/Fibroblast_activation_protein_alpha_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P02751](https://www.uniprot.org/uniprotkb/P02751) | Primary Antibody | Fibronectin | Human | Mouse | IgG1 | 2F4 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-22113AF532 | AF532 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Fibronectin_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Fluorescein/Oregon Green | Human | Rabbit | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11090 | AF488 | AB_221562 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Fluorescein_Oregon_Green_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q12951](https://www.uniprot.org/uniprotkb/Q12951) | Primary Antibody | FOXI1 | Human | Mouse | IgG1 | OTI1D4 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-70747AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-3882-457X](supporting_material/FOXI1_AF488/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | | [Q9H334](https://www.uniprot.org/uniprotkb/Q9H334) | Primary Antibody | FOXP1 | Human | Mouse | IgG2a | JC12 | [Novus Biologicals](https://www.novusbio.com/) | NB100-65125 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/FOXP1_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q99JB6](https://www.uniprot.org/uniprotkb/Q99JB6) | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-5773-82 | eF660 | AB_11220082 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_eF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-4777-42 | eF660 | AB_2574219 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_eF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-4777-41 | AF488 | AB_10852836 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q99JB6](https://www.uniprot.org/uniprotkb/Q99JB6) | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-5773-82 | AF532 | AB_11218870 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 13-4777-82 | Biotin | AB_466655 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_Biotin/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Rabbit | IgG | 1054C | [Cell IDx](https://cellidx.com/) | HI06B-005 | UT014 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_UT014/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q99JB6](https://www.uniprot.org/uniprotkb/Q99JB6) | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-5773-82 | AF488 | AB_763537 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q99JB6](https://www.uniprot.org/uniprotkb/Q99JB6) | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 12-5773-82 | PE | AB_465936 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/FOXP3_eF570/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q99JB6](https://www.uniprot.org/uniprotkb/Q99JB6) | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-5773-82 | eF660 | AB_11220082 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_eF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/FOXP3_eF570/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [Q99JB6](https://www.uniprot.org/uniprotkb/Q99JB6) | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-5773-82 | eF660 | AB_11220082 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_eF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q99JB6](https://www.uniprot.org/uniprotkb/Q99JB6) | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-5773-82 | eF570 | AB_11219073 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 14-4777-82 | Unconjugated | AB_467556 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/FOXP3_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-4777-82 | eF570 | AB_2573609 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/FOXP3_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 14-4777-82 | Unconjugated | AB_467556 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/FOXP3_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9BZS2](https://www.uniprot.org/uniprotkb/Q9BZS2) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 17-4777-42 | APC | AB_10804651 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](supporting_material/FOXP3_APC/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | +| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 14-4777-82 | Unconjugated | AB_467556 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/FOXP3_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 13-4777-82 | Biotin | AB_466655 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_Biotin/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 53-4777-41 | AF488 | AB_10852836 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-4777-42 | eF660 | AB_2574219 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_eF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/FOXP3_eF570/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 14-4777-82 | Unconjugated | AB_467556 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/FOXP3_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9BZS2](https://www.uniprot.org/uniprotkb/Q9BZS2) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 17-4777-42 | APC | AB_10804651 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/FOXP3_APC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-4777-82 | eF570 | AB_2573609 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/FOXP3_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q99JB6](https://www.uniprot.org/uniprotkb/Q99JB6) | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 50-5773-82 | eF660 | AB_11220082 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_eF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q99JB6](https://www.uniprot.org/uniprotkb/Q99JB6) | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 58-5773-82 | AF532 | AB_11218870 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9BZS1](https://www.uniprot.org/uniprotkb/Q9BZS1) | Primary Antibody | FOXP3 | Human | Rabbit | IgG | 1054C | [Cell IDx](https://cellidx.com/) | HI06B-005 | UT014 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/FOXP3_UT014/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | Francisella Tularensis, LPS | Mouse | Mouse | IgG2a | 1.B.288 | [US Biological Life Sciences](https://www.usbio.net/) | F6070-02X | Unconjugated | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Lung | Infected | Akoya Antibody Diluent | NA | NA | Yes | [0000-0001-9189-7172](supporting_material/Francisella_Tularensis,_LPS_Unconjugated/0000-0001-9189-7172.md) | NA | 0000-0001-9189-7172 | +| [Q14392](https://www.uniprot.org/uniprotkb/Q14392) | Primary Antibody | GARP | Human | Mouse | IgG2b | Plato-1 | [Enzo](https://www.enzolifesciences.com/) | ALX-804-867-C100 | Unconjugated | AB_2051806 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/GARP_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q14392](https://www.uniprot.org/uniprotkb/Q14392) | Primary Antibody | GARP | Human | Mouse | IgG2b | 7B11 | [BioLegend](https://www.biolegend.com/) | 352503 | PE | AB_10896792 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/GARP_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q14392](https://www.uniprot.org/uniprotkb/Q14392) | Primary Antibody | GARP | Human | Mouse | IgG2b | 7B11 | [BioLegend](https://www.biolegend.com/) | 352502 | Unconjugated | AB_10897447 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/GARP_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q14392](https://www.uniprot.org/uniprotkb/Q14392) | Primary Antibody | GARP | Human | Mouse | IgG2b | Plato-1 | [Enzo](https://www.enzolifesciences.com/) | ALX-804-867-C100 | Unconjugated | AB_2051806 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/GARP_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P23771](https://www.uniprot.org/uniprotkb/P23771) | Primary Antibody | GATA-3 | Human | Mouse | IgG1 | L50-823 | [BD Biosciences](https://www.bdbiosciences.com/) | 560077 | AF488 | AB_1645303 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/GATA-3_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P14136](https://www.uniprot.org/uniprotkb/P14136) | Primary Antibody | GFAP | Mouse | Mouse | IgG2b | SMI 25 | [BioLegend](https://www.biolegend.com/) | 837504 | AF647 | AB_2632641 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/GFAP_AF647/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [P03995](https://www.uniprot.org/uniprotkb/P03995) | Primary Antibody | GFAP | Mouse | Mouse | IgG1 | GA5 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-9892-82 | eF570 | AB_2573656 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](supporting_material/GFAP_eF570/0000-0002-6944-6997.md) | NA | 0000-0002-6944-6997 | | [Q58EE9](https://www.uniprot.org/uniprotkb/Q58EE9) | Primary Antibody | GFAP | Zebrafish | Mouse | IgG2b | SMI 25 | [BioLegend](https://www.biolegend.com/) | 837508 | AF488 | AB_2728555 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | [0009-0000-2047-4228](supporting_material/GFAP_AF488/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | +| [P14136](https://www.uniprot.org/uniprotkb/P14136) | Primary Antibody | GFAP | Mouse | Mouse | IgG2b | SMI 25 | [BioLegend](https://www.biolegend.com/) | 837504 | AF647 | AB_2632641 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/GFAP_AF647/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [P42212](https://www.uniprot.org/uniprotkb/P42212) | Primary Antibody | GFP | Jellyfish GFP transgene | Chicken | IgY | Monoclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A10262 | Unconjugated | AB_2534023 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/GFP_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [Q76IQ4](https://www.uniprot.org/uniprotkb/Q76IQ4) | Primary Antibody | Ghrelin | Human | Mouse | IgG2b | 891240 | [R&D Systems](https://www.rndsystems.com/) | MAB8149 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Ghrelin_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | GL-7 | Mouse | Rat | IgM | GL7 | [BD Biosciences](https://www.bdbiosciences.com/) | 561530 | PE | AB_10715834 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/GL-7_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | GL7 | Mouse | Rat | IgM | GL7 | [BD Biosciences](https://www.bdbiosciences.com/) | 561530 | PE | AB_10715834 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/GL7_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [A0A8C6NQP0](https://www.uniprot.org/uniprotkb/A0A8C6NQP0) | Primary Antibody | Glutamine synthetase | Turquoise Killifish | Mouse | IgG1 | 1B6G4 | [Proteintech](https://www.ptglab.com/) | CL488-66323 | CoraLite Plus AF488 | AB_2883309 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | [0009-0000-2047-4228](supporting_material/Glutamine_synthetase_CoraLite_Plus_AF488/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| [P15104](https://www.uniprot.org/uniprotkb/P15104) | Primary Antibody | Glutamine synthetase | Mouse | Mouse | IgG1 | 1B6G4 | [Proteintech](https://www.ptglab.com/) | CL488-66323 | CoraLite Plus AF488 | AB_2883309 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/Glutamine_synthetase_CoraLite_Plus_AF488/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | -| [P15104](https://www.uniprot.org/uniprotkb/P15104) | Primary Antibody | Glutamine synthetase | Human | Mouse | IgG2a | OTI1F4 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-70834AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/Glutamine_synthetase_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P15104](https://www.uniprot.org/uniprotkb/P15104) | Primary Antibody | Glutamine synthetase | Human | Rabbit | IgG | EPR13022(B) | [Abcam](https://www.abcam.com/) | ab302584 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/Glutamine_synthetase_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P15104](https://www.uniprot.org/uniprotkb/P15104) | Primary Antibody | Glutamine synthetase | Mouse | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab49873 | Unconjugated | AB_880241 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | NA | Yes | [0000-0003-1431-1398](supporting_material/Glutamine_synthetase_Unconjugated/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | +| [A0A8C6NQP0](https://www.uniprot.org/uniprotkb/A0A8C6NQP0) | Primary Antibody | Glutamine synthetase | Turquoise Killifish | Mouse | IgG1 | 1B6G4 | [Proteintech](https://www.ptglab.com/) | CL488-66323 | CoraLite Plus AF488 | AB_2883309 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | [0009-0000-2047-4228](supporting_material/Glutamine_synthetase_CoraLite_Plus_AF488/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [P15104](https://www.uniprot.org/uniprotkb/P15104) | Primary Antibody | Glutamine synthetase | Human | Mouse | IgG2a | OTI1F4 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-70834AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/Glutamine_synthetase_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P51121](https://www.uniprot.org/uniprotkb/P51121) | Primary Antibody | Glutamine synthetase | African Clawed Frog (Xenopus laevis) | Mouse | IgG1 | 1B6G4 | [Proteintech](https://www.ptglab.com/) | CL488-66323 | CoraLite Plus AF488 | AB_2883309 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | [0009-0000-2047-4228](supporting_material/Glutamine_synthetase_CoraLite_Plus_AF488/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [P15104](https://www.uniprot.org/uniprotkb/P15104) | Primary Antibody | Glutamine synthetase | Human | Rabbit | IgG | EPR13022(B) | [Abcam](https://www.abcam.com/) | Ab302584 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/Glutamine_synthetase_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P15104](https://www.uniprot.org/uniprotkb/P15104) | Primary Antibody | Glutamine synthetase | Mouse | Mouse | IgG1 | 1B6G4 | [Proteintech](https://www.ptglab.com/) | CL488-66323 | CoraLite Plus AF488 | AB_2883309 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/Glutamine_synthetase_CoraLite_Plus_AF488/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [Q7ZVF2](https://www.uniprot.org/uniprotkb/Q7ZVF2) | Primary Antibody | Glutamine synthetase | Zebrafish | Mouse | IgG1 | 1B6G4 | [Proteintech](https://www.ptglab.com/) | CL488-66323 | CoraLite Plus AF488 | AB_2883309 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | [0009-0000-2047-4228](supporting_material/Glutamine_synthetase_CoraLite_Plus_AF488/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| [P15104](https://www.uniprot.org/uniprotkb/P15104) | Primary Antibody | Glutamine synthetase | Human | Mouse | IgG2bc | GLUL/6600 | [Novus Biologicals](https://www.novusbio.com/) | NBP3-14023AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/Glutamine_synthetase_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P15104](https://www.uniprot.org/uniprotkb/P15104) | Primary Antibody | Glutamine synthetase | Mouse | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab49873 | Unconjugated | AB_880241 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | NA | Yes | [0000-0003-1431-1398](supporting_material/Glutamine_synthetase_Unconjugated/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | +| [P15104](https://www.uniprot.org/uniprotkb/P15104) | Primary Antibody | Glutamine synthetase | Human | Mouse | IgG2a | OTI1F4 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-70834AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/Glutamine_synthetase_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P15104](https://www.uniprot.org/uniprotkb/P15104) | Primary Antibody | Glutamine synthetase | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab49873 | Unconjugated | AB_880241 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-1431-1398](supporting_material/Glutamine_synthetase_Unconjugated/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | +| [P15104](https://www.uniprot.org/uniprotkb/P15104) | Primary Antibody | Glutamine synthetase | Human | Mouse | IgG2bc | GLUL/6600 | [Novus Biologicals](https://www.novusbio.com/) | NBP3-14023AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/Glutamine_synthetase_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P51121](https://www.uniprot.org/uniprotkb/P51121) | Primary Antibody | Glutamine synthetase | African Clawed Frog (Xenopus laevis) | Mouse | IgG1 | 1B6G4 | [Proteintech](https://www.ptglab.com/) | CL488-66323 | CoraLite Plus AF488 | AB_2883309 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | [0009-0000-2047-4228](supporting_material/Glutamine_synthetase_CoraLite_Plus_AF488/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [P02724](https://www.uniprot.org/uniprotkb/P02724) | Primary Antibody | Glycophorin A | Human | Rabbit | IgG | EPR8200 | [Abcam](https://www.abcam.com/) | ab218372 (Unconjugated) | AF555 | AB_2892758 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-3882-457X](supporting_material/Glycophorin_A_AF555/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | | [P02724](https://www.uniprot.org/uniprotkb/P02724) | Primary Antibody | Glycophorin A | Human | Rabbit | IgG | EPR8200 | [Abcam](https://www.abcam.com/) | ab218372 (Unconjugated) | AF555 | AB_2892758 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Glycophorin_A_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q90WX5](https://www.uniprot.org/uniprotkb/Q90WX5) | Primary Antibody | GNAT2 | Zebrafish | Rabbit | IgG | Polyclonal | [MBL International Corporation](https://mblintl.com/) | PM075 | Unconjugated | AB_2864374 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/GNAT2_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | NA | Primary Antibody | Go-alpha | Zebrafish | Mouse | IgG1 | 2A | [MilliporeSigma](https://www.sigmaaldrich.com/) | MAB3073 | Unconjugated | AB_94671 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Go-alpha_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11055 | AF488 | AB_2534102 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Goat IgG (H+L) | Canine | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21447 | AF647 | AB_2535864 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0001-5088-7808](supporting_material/Goat_IgG__H+L__AF647/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | -| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21432 | AF555 | AB_2535853 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11055 | AF488 | AB_2534102 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11058 | AF594 | AB_2534105 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-32930 | AF800 (Plus) | AB_2762842 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF800__Plus_/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21447 | AF647 | AB_2535864 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | SA5-10091 | DL755 | AB_2556671 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__DL755/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab175745 | AF750 | AB_2924800 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF750/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A32816 | AF555 (Plus) | AB_2762839 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/Goat_IgG__H+L__AF555__Plus_/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | -| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11056 | AF546 | AB_2534103 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF546/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-32930 | AF800 (Plus) | AB_2762842 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF800__Plus_/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | SA5-10091 | DL755 | AB_2556671 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__DL755/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab175745 | AF750 | AB_2924800 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF750/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21447 | AF647 | AB_2535864 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21432 | AF555 | AB_2535853 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21432 | AF555 | AB_2535853 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21432 | AF555 | AB_2535853 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21084 | AF680 | AB_2535741 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21084 | AF680 | AB_2535741 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11056 | AF546 | AB_2534103 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF546/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11055 | AF488 | AB_2534102 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21432 | AF555 | AB_2535853 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21084 | AF680 | AB_2535741 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-3882-457X](supporting_material/Goat_IgG__H+L__AF680/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | +| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11055 | AF488 | AB_2534102 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11055 | AF488 | AB_2534102 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21432 | AF555 | AB_2535853 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21432 | AF555 | AB_2535853 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21432 | AF555 | AB_2535853 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21084 | AF680 | AB_2535741 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11058 | AF594 | AB_2534105 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/Goat_IgG__H+L__AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Goat IgG (H+L) | Canine | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21447 | AF647 | AB_2535864 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0001-5088-7808](supporting_material/Goat_IgG__H+L__AF647/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | | [Q62011](https://www.uniprot.org/uniprotkb/Q62011) | Primary Antibody | gp38 | Mouse | Syrian Hamster | IgG | 8.1.1 | [BioLegend](https://www.biolegend.com/) | 127401 | Unconjugated | AB_1089186 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/gp38_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P10144](https://www.uniprot.org/uniprotkb/P10144) | Primary Antibody | Granzyme B | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab4059 | Unconjugated | AB_304251 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/Granzyme_B_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | Granzyme B | Canine | Mouse | IgG1 | GB12 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MHGB04 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/Granzyme_B_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | -| [P10144](https://www.uniprot.org/uniprotkb/P10144) | Primary Antibody | Granzyme B | Human | Rabbit | IgG | EP230 | [Cell Marque](https://www.cellmarque.com/) | 262R-17 | Unconjugated | AB_2889344 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/Granzyme_B_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [F7CLT4](https://www.uniprot.org/uniprotkb/F7CLT4) | Primary Antibody | Granzyme B | Rhesus Macaque | Mouse | IgG2a | GrB-7 | [Agilent](https://www.agilent.com/en/dako-products) | M7235 | Unconjugated | AB_2114697 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/Granzyme_B_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P10144](https://www.uniprot.org/uniprotkb/P10144) | Primary Antibody | Granzyme B | Human | Rabbit | IgG | EP230 | [Cell Marque](https://www.cellmarque.com/) | 262R-17 | Unconjugated | AB_2889344 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/Granzyme_B_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P10144](https://www.uniprot.org/uniprotkb/P10144) | Primary Antibody | Granzyme B | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab4059 | Unconjugated | AB_304251 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/Granzyme_B_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Hamster IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21451 | AF647 | AB_2535868 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Hamster_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q64525](https://www.uniprot.org/uniprotkb/Q64525) | Primary Antibody | Histone H2B | Mouse | Rat | IgG2a | W17025A | [BioLegend](https://www.biolegend.com/) | 606301 | Unconjugated | AB_2728465 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0002-5187-810X](supporting_material/Histone_H2B_Unconjugated/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | | [P02302](https://www.uniprot.org/uniprotkb/P02302) | Primary Antibody | Histone H3 | Mouse | Mouse | IgG3 | 1B1-B2 | [BioLegend](https://www.biolegend.com/) | 819406 | AF594 | AB_2715803 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0002-5187-810X](supporting_material/Histone_H3_AF594/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | | [P68431](https://www.uniprot.org/uniprotkb/P68431) | Primary Antibody | Histone H3 (citrulline R2 + R8 + R17) | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab5103 | Unconjugated | AB_304752 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-3882-457X](supporting_material/Histone_H3__citrulline_R2_+_R8_+_R17__Unconjugated/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47670 | PE | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/HLA-DR_PE/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | LN-3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47670AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307622 | AF647 | AB_493177 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1032136 | iF594 | AB_2892750 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307620 | AF488 | AB_493175 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47670 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [Novus Biologicals](https://www.novusbio.com/) | NB100-77855AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/HLA-DR_AF750/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1032136 | iF594 | AB_2892750 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1032136 | iF594 | AB_2892750 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307620 | AF488 | AB_493175 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | [Abcam](https://www.abcam.com/) | ab20181 | AF647 | AB_445401 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/HLA-DR_AF647/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307620 | AF488 | AB_493175 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307602 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | HLA-DR | Canine | Mouse | IgG1 | TAL 1B5 | [Abcam](https://www.abcam.com/) | ab176408 (Unconjugated) | iF594 | NA | Custom | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0001-5088-7808](supporting_material/HLA-DR_iF594/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307622 | AF647 | AB_493177 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307625 | AF700 | AB_493770 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/HLA-DR_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | LN-3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47670AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307625 | AF700 | AB_493770 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/HLA-DR_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307622 | AF647 | AB_493177 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307602 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47670AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47670AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/HLA-DR_AF750/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307620 | AF488 | AB_493175 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307620 | AF488 | AB_493175 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307620 | AF488 | AB_493175 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1032136 | iF594 | AB_2892750 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1032136 | iF594 | AB_2892750 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | LN-3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47670PE | PE | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | LN-3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47670AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | [Abcam](https://www.abcam.com/) | ab176408 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/HLA-DR_iF594/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | [Abcam](https://www.abcam.com/) | ab20181 | AF647 | AB_445401 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/HLA-DR_AF647/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | | [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307619 | AF488 | AB_493176 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | [Abcam](https://www.abcam.com/) | ab176408 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/HLA-DR_iF594/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47670AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [Novus Biologicals](https://www.novusbio.com/) | NB100-77855AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/HLA-DR_AF750/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307620 | AF488 | AB_493175 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47670 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | [Novus Biologicals](https://www.novusbio.com/) | NB600-989AF532-0.1ml | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/HLA-DR_AF532/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307620 | AF488 | AB_493175 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | [Abcam](https://www.abcam.com/) | ab176408 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/HLA-DR_iF594/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | [Abcam](https://www.abcam.com/) | ab176408 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/HLA-DR_iF594/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | LN-3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47670AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [BioLegend](https://www.biolegend.com/) | 307622 | AF647 | AB_493177 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47670 | PE | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/HLA-DR_PE/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | +| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | [Caprico Biotechnologies](https://www.capricobio.com/) | 1032136 | iF594 | AB_2892750 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47670AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/HLA-DR_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01903](https://www.uniprot.org/uniprotkb/P01903) | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-47670AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/HLA-DR_AF750/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01911](https://www.uniprot.org/uniprotkb/P01911) | Primary Antibody | HLA-DRB | Human | Mouse | IgG2b | SPM288 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-45312-0.02mg | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/HLA-DRB_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P63158](https://www.uniprot.org/uniprotkb/P63158) | Primary Antibody | HMGB-1 | Mouse | Mouse | IgG2 | 3E8 | [BioLegend](https://www.biolegend.com/) | 651406 | AF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0002-5187-810X](supporting_material/HMGB-1_AF594/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | -| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | [Biotium](https://biotium.com/) | 40046 | NA | NA | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Hoechst_33342_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | [Biotium](https://biotium.com/) | 40046 | NA | NA | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Hoechst_33342_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | [Biotium](https://biotium.com/) | 40046 | NA | NA | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-5187-810X](supporting_material/Hoechst_33342_NA/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | +| NA | Nuclear Dye | Hoechst 33342 | Rhesus Macaque | NA | NA | NA | [Biotium](https://biotium.com/) | 40046 | NA | NA | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | [0009-0004-2746-8743](supporting_material/Hoechst_33342_NA/0009-0004-2746-8743.md) | NA | 0009-0004-2746-8743 | +| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | [Biotium](https://biotium.com/) | 40046 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Hoechst_33342_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | [Biotium](https://biotium.com/) | 40046 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-3882-457X](supporting_material/Hoechst_33342_NA/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | | NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | [Biotium](https://biotium.com/) | 40046 | NA | NA | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Hoechst_33342_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | [Biotium](https://biotium.com/) | 40046 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Hoechst_33342_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Nuclear Dye | Hoechst 33342 | Rhesus Macaque | NA | NA | NA | [Biotium](https://biotium.com/) | 40046 | NA | NA | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | [0009-0004-2746-8743](supporting_material/Hoechst_33342_NA/0009-0004-2746-8743.md) | NA | 0009-0004-2746-8743 | +| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | [Biotium](https://biotium.com/) | 40046 | NA | NA | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-5187-810X](supporting_material/Hoechst_33342_NA/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | +| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | [Biotium](https://biotium.com/) | 40046 | NA | NA | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Hoechst_33342_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | [Biotium](https://biotium.com/) | 40046 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Hoechst_33342_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | HuC/D | African Clawed Frog (Xenopus laevis) | Mouse | IgG2b | 16A11 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A21271 | Unconjugated | AB_221448 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/HuC_D_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | +| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | [Biotium](https://biotium.com/) | 40046 | NA | NA | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Hoechst_33342_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | HuC/D | Zebrafish | Mouse | IgG2b | 16A11 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A21271 | Unconjugated | AB_221448 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/HuC_D_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | NA | Primary Antibody | HuC/D | Turquoise Killifish | Mouse | IgG2b | 16A11 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A21271 | Unconjugated | AB_221448 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/HuC_D_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | +| NA | Primary Antibody | HuC/D | African Clawed Frog (Xenopus laevis) | Mouse | IgG2b | 16A11 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A21271 | Unconjugated | AB_221448 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/HuC_D_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [Q14576](https://www.uniprot.org/uniprotkb/Q14576) | Primary Antibody | HuC/HuD neuronal protein | Human | Mouse | IgG2b | 16A11 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A21271 | Unconjugated | AB_221448 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | [0000-0003-2088-8310](supporting_material/HuC_HuD_neuronal_protein_Unconjugated/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [Q9EQX4](https://www.uniprot.org/uniprotkb/Q9EQX4) | Primary Antibody | Iba-1 | Mouse | Rabbit | IgG | Polyclonal | [FUJIFILM Wako Chemicals U.S.A. Corporation](https://labchem-wako.fujifilm.com) | 019-19741 | NA | AB_839504 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](supporting_material/Iba-1_NA/0000-0002-6944-6997.md) | NA | 0000-0002-6944-6997 | | [P55008](https://www.uniprot.org/uniprotkb/P55008) | Primary Antibody | Iba1 | Human | Goat | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab5076 | Unconjugated | AB_2224402 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | [0000-0003-2088-8310](supporting_material/Iba1_Unconjugated/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | -| [Q9Y6W8](https://www.uniprot.org/uniprotkb/Q9Y6W8) | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | [BioLegend](https://www.biolegend.com/) | 313515 | AF647 | AB_2122583 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/ICOS_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9Y6W8](https://www.uniprot.org/uniprotkb/Q9Y6W8) | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | [BioLegend](https://www.biolegend.com/) | 313527 | AF700 | AB_2566125 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/ICOS_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9Y6W8](https://www.uniprot.org/uniprotkb/Q9Y6W8) | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | [BioLegend](https://www.biolegend.com/) | 313514 | AF488 | AB_2122584 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/ICOS_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9Y6W8](https://www.uniprot.org/uniprotkb/Q9Y6W8) | Primary Antibody | ICOS | Human | Rabbit | IgG | SP98 | [Abcam](https://www.abcam.com/) | ab105227 | Unconjugated | AB_10710236 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/ICOS_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9Y6W8](https://www.uniprot.org/uniprotkb/Q9Y6W8) | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | [BioLegend](https://www.biolegend.com/) | 313514 | AF488 | AB_2122584 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/ICOS_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9Y6W8](https://www.uniprot.org/uniprotkb/Q9Y6W8) | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | [BioLegend](https://www.biolegend.com/) | 313514 | AF488 | AB_2122584 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/ICOS_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9Y6W8](https://www.uniprot.org/uniprotkb/Q9Y6W8) | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | [BioLegend](https://www.biolegend.com/) | 313515 | AF647 | AB_2122583 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/ICOS_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9Y6W8](https://www.uniprot.org/uniprotkb/Q9Y6W8) | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | [BioLegend](https://www.biolegend.com/) | 313527 | AF700 | AB_2566125 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/ICOS_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01878](https://www.uniprot.org/uniprotkb/P01878) | Primary Antibody | IgA | Mouse | Goat | IgG | Polyclonal | [SouthernBiotech](https://www.southernbiotech.com/) | 1040-32 | AF555 | AB_2794378 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/IgA_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01878](https://www.uniprot.org/uniprotkb/P01878) | Primary Antibody | IgA | Mouse | Goat | IgG | Polyclonal | [SouthernBiotech](https://www.southernbiotech.com/) | 1040-32 | AF555 | AB_2794378 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/IgA_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01876](https://www.uniprot.org/uniprotkb/P01876) | Primary Antibody | IgA1 | Human | Mouse | IgG1 | B3506B4 | [SouthernBiotech](https://www.southernbiotech.com/) | 9130-31 | AF647 | AB_2796658 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/IgA1_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01876](https://www.uniprot.org/uniprotkb/P01876) | Primary Antibody | IgA1 | Human | Mouse | IgG1 | B3506B4 | [SouthernBiotech](https://www.southernbiotech.com/) | 9130-31 | AF647 | AB_2796658 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/IgA1_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01876](https://www.uniprot.org/uniprotkb/P01876) | Primary Antibody | IgA1 | Human | Mouse | IgG1 | B3506B4 | [SouthernBiotech](https://www.southernbiotech.com/) | 9130-31 | AF647 | AB_2796658 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/IgA1_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01877](https://www.uniprot.org/uniprotkb/P01877) | Primary Antibody | IgA2 | Human | Mouse | IgG1 | A9604D2 | [SouthernBiotech](https://www.southernbiotech.com/) | 9140-30 | AF488 | AB_2796665 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/IgA2_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01877](https://www.uniprot.org/uniprotkb/P01877) | Primary Antibody | IgA2 | Human | Mouse | IgG1 | A9604D2 | [SouthernBiotech](https://www.southernbiotech.com/) | 9140-30 | AF488 | AB_2796665 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/IgA2_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01880](https://www.uniprot.org/uniprotkb/P01880) | Primary Antibody | IgD | Human | Mouse | IgG2a | IA6-2 | [BioLegend](https://www.biolegend.com/) | 348216 | AF488 | AB_11150595 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/IgD_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01881](https://www.uniprot.org/uniprotkb/P01881) | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | [BioLegend](https://www.biolegend.com/) | 405718 | AF488 | AB_10730619 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/IgD_AF488/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | -| NA | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | [BioLegend](https://www.biolegend.com/) | 405718 | AF488 | AB_10730619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/IgD_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01880](https://www.uniprot.org/uniprotkb/P01880) | Primary Antibody | IgD | Human | Mouse | IgG2a | IA6-2 | [BioLegend](https://www.biolegend.com/) | 348229 | AF700 | AB_2563334 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/IgD_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01881](https://www.uniprot.org/uniprotkb/P01881) | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | [BioLegend](https://www.biolegend.com/) | 405723 | BV510 | AB_2562742 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0002-1461-0999](supporting_material/IgD_BV510/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | -| [P01880](https://www.uniprot.org/uniprotkb/P01880) | Primary Antibody | IgD | Human | Mouse | IgG2a | IA6-2 | [BioLegend](https://www.biolegend.com/) | 348216 | AF488 | AB_11150595 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/IgD_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P01881](https://www.uniprot.org/uniprotkb/P01881) | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | [BioLegend](https://www.biolegend.com/) | 405730 | AF700 | AB_2563341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/IgD_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P01881](https://www.uniprot.org/uniprotkb/P01881) | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | [BioLegend](https://www.biolegend.com/) | 405730 | AF700 | AB_2563341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/IgD_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01880](https://www.uniprot.org/uniprotkb/P01880) | Primary Antibody | IgD | Human | Mouse | IgG2a | IA6-2 | [BioLegend](https://www.biolegend.com/) | 348216 | AF488 | AB_11150595 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/IgD_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01881](https://www.uniprot.org/uniprotkb/P01881) | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | [BioLegend](https://www.biolegend.com/) | 405723 | BV510 | AB_2562742 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0002-1461-0999](supporting_material/IgD_BV510/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | | [P01881](https://www.uniprot.org/uniprotkb/P01881) | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | [BioLegend](https://www.biolegend.com/) | 405740 | AF594 | AB_2565572 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/IgD_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01880](https://www.uniprot.org/uniprotkb/P01880) | Primary Antibody | IgD | Human | Mouse | IgG2a | IA6-2 | [BioLegend](https://www.biolegend.com/) | 348229 | AF700 | AB_2563334 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/IgD_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | [BioLegend](https://www.biolegend.com/) | 405718 | AF488 | AB_10730619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/IgD_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01881](https://www.uniprot.org/uniprotkb/P01881) | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | [BioLegend](https://www.biolegend.com/) | 405730 | AF700 | AB_2563341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/IgD_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P01881](https://www.uniprot.org/uniprotkb/P01881) | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | [BioLegend](https://www.biolegend.com/) | 405718 | AF488 | AB_10730619 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/IgD_AF488/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | | [P06336](https://www.uniprot.org/uniprotkb/P06336) | Primary Antibody | IgE | Mouse | Rat | IgG1 | RME-1 | [BioLegend](https://www.biolegend.com/) | 406908 | PE | AB_493290 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-1461-0999](supporting_material/IgE_PE/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | | [P01868](https://www.uniprot.org/uniprotkb/P01868) | Primary Antibody | IgG1 | Mouse | Rat | IgG | RMG1-1 | [BioLegend](https://www.biolegend.com/) | 406616 | BV421 | AB_2562234 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0002-1461-0999](supporting_material/IgG1_BV421/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | -| [P07476](https://www.uniprot.org/uniprotkb/P07476) | Primary Antibody | Involucrin | Human | Mouse | IgG1 | SY5 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-21748 AF488 | AF488 | AB_2128119 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](supporting_material/Involucrin_AF488/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | | [P07476](https://www.uniprot.org/uniprotkb/P07476) | Primary Antibody | Involucrin | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab53112 | Unconjugated | AB_880813 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-2950-2683](supporting_material/Involucrin_Unconjugated/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | -| [Q64287](https://www.uniprot.org/uniprotkb/Q64287) | Primary Antibody | IRF4 | Mouse | Rat | IgG1 | 3.E.4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 11-9858-82 | FITC | AB_2572538 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/IRF4_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q15306](https://www.uniprot.org/uniprotkb/Q15306) | Primary Antibody | IRF4 | Human | Rat | IgG1 | IRF4.3E4 | [BioLegend](https://www.biolegend.com/) | 646404 | PE | AB_2563005 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/IRF4_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P07476](https://www.uniprot.org/uniprotkb/P07476) | Primary Antibody | Involucrin | Human | Mouse | IgG1 | SY5 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-21748 AF488 | AF488 | AB_2128119 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](supporting_material/Involucrin_AF488/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | +| [Q15306](https://www.uniprot.org/uniprotkb/Q15306) | Primary Antibody | IRF4 | Human | Rabbit | IgG | EP5699 | [Abcam](https://www.abcam.com/) | ab252024 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/IRF4_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q15306](https://www.uniprot.org/uniprotkb/Q15306) | Primary Antibody | IRF4 | Human | Rat | IgG1 | IRF4.3E4 | [BioLegend](https://www.biolegend.com/) | 646404 | PE | AB_2563005 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/IRF4_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q15306](https://www.uniprot.org/uniprotkb/Q15306) | Primary Antibody | IRF4 | Human | Rat | IgG1 | IRF4.3E4 | [BioLegend](https://www.biolegend.com/) | 646404 | PE | AB_2563005 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/IRF4_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q64287](https://www.uniprot.org/uniprotkb/Q64287) | Primary Antibody | IRF4 | Mouse | Rat | IgG1 | 3.E.4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 11-9858-82 | FITC | AB_2572538 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/IRF4_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q15306](https://www.uniprot.org/uniprotkb/Q15306) | Primary Antibody | IRF4 | Human | Sheep | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF5525 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/IRF4_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q15306](https://www.uniprot.org/uniprotkb/Q15306) | Primary Antibody | IRF4 | Human | Mouse | IgG1 | MUM1p | [Novus Biologicals](https://www.novusbio.com/) | NB200-356 | Unconjugated | AB_10003502 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/IRF4_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q15306](https://www.uniprot.org/uniprotkb/Q15306) | Primary Antibody | IRF4 | Human | Mouse | IgG1 | MUM1p | [Novus Biologicals](https://www.novusbio.com/) | NB200-356 | Unconjugated | AB_10003502 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/IRF4_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q15306](https://www.uniprot.org/uniprotkb/Q15306) | Primary Antibody | IRF4 | Human | Mouse | IgG1 | 4G10 | [Novus Biologicals](https://www.novusbio.com/) | NBP1-47396 | Unconjugated | AB_10010349 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/IRF4_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q15306](https://www.uniprot.org/uniprotkb/Q15306) | Primary Antibody | IRF4 | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NBP1-82814 | Unconjugated | AB_11038596 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/IRF4_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q15306](https://www.uniprot.org/uniprotkb/Q15306) | Primary Antibody | IRF4 | Human | Rabbit | IgG | EP5699 | [Abcam](https://www.abcam.com/) | ab252024 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/IRF4_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q15306](https://www.uniprot.org/uniprotkb/Q15306) | Primary Antibody | IRF4 | Human | Mouse | IgG1 | MUM1p | [Novus Biologicals](https://www.novusbio.com/) | NB200-356 | Unconjugated | AB_10003502 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/IRF4_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q15306](https://www.uniprot.org/uniprotkb/Q15306) | Primary Antibody | IRF4 | Human | Mouse | IgG1 | MUM1p | [Novus Biologicals](https://www.novusbio.com/) | NB200-356 | Unconjugated | AB_10003502 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/IRF4_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q02556](https://www.uniprot.org/uniprotkb/Q02556) | Primary Antibody | IRF8 | Human | Rabbit | IgG | E6J8Q | [Cell Signaling Technology](https://www.cellsignal.com/) | 80174S | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](supporting_material/IRF8_AF488/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | | NA | Lectin | Isolectin GS-IB4 | Human | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | I21412 | AF568 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | [0000-0003-2088-8310](supporting_material/Isolectin_GS-IB4_AF568/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | +| NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/JOJO-1_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/JOJO-1_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/JOJO-1_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/JOJO-1_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/JOJO-1_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/JOJO-1_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/JOJO-1_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P02533](https://www.uniprot.org/uniprotkb/P02533) | Primary Antibody | Keratin 14 | Human | Chicken | IgY | Poly9060 | [BioLegend](https://www.biolegend.com/) | 906004 (Unconjugated) | AF647 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/Keratin_14_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P13647](https://www.uniprot.org/uniprotkb/P13647) | Primary Antibody | Keratin 5 | Human | Rabbit | IgG | Polyclonal | [BioLegend](https://www.biolegend.com/) | 905504 (Unconjugated) | AF532 | AB_3095835 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Keratin_5_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P79192](https://www.uniprot.org/uniprotkb/P79192) | Primary Antibody | Ki-67 | Rhesus Macaque | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 563462 | BV510 | AB_2738221 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/Ki-67_BV510/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P79192](https://www.uniprot.org/uniprotkb/P79192) | Primary Antibody | Ki-67 | Rhesus Macaque | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 562899 | BV421 | AB_2686897 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/Ki-67_BV421/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [P79192](https://www.uniprot.org/uniprotkb/P79192) | Primary Antibody | Ki-67 | Rhesus Macaque | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 558616 | AF488 | AB_647087 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/Ki-67_AF488/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [P79192](https://www.uniprot.org/uniprotkb/P79192) | Primary Antibody | Ki-67 | Rhesus Macaque | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 558615 | AF647 | AB_647130 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/Ki-67_AF647/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [Q9BYG3](https://www.uniprot.org/uniprotkb/Q9BYG3) | Primary Antibody | Ki-67 | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NB500-170b | Biotin | AB_1660247 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_Biotin/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9BYG3](https://www.uniprot.org/uniprotkb/Q9BYG3) | Primary Antibody | Ki-67 | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NB500-170b | Biotin | AB_1660247 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_Biotin/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P79192](https://www.uniprot.org/uniprotkb/P79192) | Primary Antibody | Ki-67 | Rhesus Macaque | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 562899 | BV421 | AB_2686897 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/Ki-67_BV421/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [P79192](https://www.uniprot.org/uniprotkb/P79192) | Primary Antibody | Ki-67 | Rhesus Macaque | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 563462 | BV510 | AB_2738221 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/Ki-67_BV510/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [Q9BYG3](https://www.uniprot.org/uniprotkb/Q9BYG3) | Primary Antibody | Ki-67 | Human | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [E9PVX6](https://www.uniprot.org/uniprotkb/E9PVX6) | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 558616 | AF488 | AB_647087 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_AF488/0000-0003-4379-8967.md), [0000-0002-1461-0999](supporting_material/Ki-67_AF488/0000-0002-1461-0999.md) | NA | 0000-0003-4379-8967 | +| [E9PVX6](https://www.uniprot.org/uniprotkb/E9PVX6) | Primary Antibody | Ki-67 | Mouse | Rat | IgG2a | SolA15 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 48-5698-80 | eF450 | AB_11151155 | Stock | Ce3D-IBEX | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 1 hour | Yes | [0000-0002-0835-911X](supporting_material/Ki-67_eF450/0000-0002-0835-911X.md) | NA | 0000-0002-0835-911X | +| [Q9BYG3](https://www.uniprot.org/uniprotkb/Q9BYG3) | Primary Antibody | Ki-67 | Human | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9BYG3](https://www.uniprot.org/uniprotkb/Q9BYG3) | Primary Antibody | Ki-67 | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NB500-170b | Biotin | AB_1660247 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_Biotin/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9BYG3](https://www.uniprot.org/uniprotkb/Q9BYG3) | Primary Antibody | Ki-67 | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NB500-170 | Unconjugated | AB_10001977 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9BYG3](https://www.uniprot.org/uniprotkb/Q9BYG3) | Primary Antibody | Ki-67 | Human | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [E9PVX6](https://www.uniprot.org/uniprotkb/E9PVX6) | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 558616 | AF488 | AB_647087 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_AF488/0000-0003-4379-8967.md), [0000-0002-1461-0999](supporting_material/Ki-67_AF488/0000-0002-1461-0999.md) | NA | 0000-0003-4379-8967 | | [E9PVX6](https://www.uniprot.org/uniprotkb/E9PVX6) | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [E9PVX6](https://www.uniprot.org/uniprotkb/E9PVX6) | Primary Antibody | Ki-67 | Mouse | Rat | IgG2a | SolA15 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 48-5698-80 | eF450 | AB_11151155 | Stock | Ce3D-IBEX | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 1 hour | Yes | [0000-0002-0835-911X](supporting_material/Ki-67_eF450/0000-0002-0835-911X.md) | NA | 0000-0002-0835-911X | -| [E9PVX7](https://www.uniprot.org/uniprotkb/E9PVX7) | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [E9PVX6](https://www.uniprot.org/uniprotkb/E9PVX6) | Primary Antibody | Ki-67 | Mouse | Rat | IgG2a | SolA15 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 606-5698-82 | AF660 | AB_2896286 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](supporting_material/Ki-67_AF660/0000-0002-6944-6997.md) | NA | 0000-0002-6944-6997 | +| [E9PVX7](https://www.uniprot.org/uniprotkb/E9PVX7) | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [E9PVX8](https://www.uniprot.org/uniprotkb/E9PVX8) | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9BYG3](https://www.uniprot.org/uniprotkb/Q9BYG3) | Primary Antibody | Ki-67 | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NB500-170b | Biotin | AB_1660247 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_Biotin/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [E9PVX9](https://www.uniprot.org/uniprotkb/E9PVX9) | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_AF700/0000-0003-4379-8967.md), [0000-0003-1118-7432](supporting_material/Ki-67_AF700/0000-0003-1118-7432.md) | NA | 0000-0003-4379-8967 | | [Q9BYG3](https://www.uniprot.org/uniprotkb/Q9BYG3) | Primary Antibody | Ki-67 | Human | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9BYG3](https://www.uniprot.org/uniprotkb/Q9BYG3) | Primary Antibody | Ki-67 | Human | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9BYG3](https://www.uniprot.org/uniprotkb/Q9BYG3) | Primary Antibody | Ki-67 | Human | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [O88713](https://www.uniprot.org/uniprotkb/O88713) | Primary Antibody | KLRG1 | Mouse | Syrian Hamster | IgG2 | 2F1 | [BD Biosciences](https://www.bdbiosciences.com/) | 561619 | AF488 | AB_10898017 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/KLRG1_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [O88713](https://www.uniprot.org/uniprotkb/O88713) | Primary Antibody | KLRG1 | Mouse | Syrian Hamster | IgG2 | 2F1 | [BD Biosciences](https://www.bdbiosciences.com/) | 561619 | AF488 | AB_10898017 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/KLRG1_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P79192](https://www.uniprot.org/uniprotkb/P79192) | Primary Antibody | Ki-67 | Rhesus Macaque | Mouse | IgG1 | B56 | [BD Biosciences](https://www.bdbiosciences.com/) | 558615 | AF647 | AB_647130 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/Ki-67_AF647/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [Q9BYG3](https://www.uniprot.org/uniprotkb/Q9BYG3) | Primary Antibody | Ki-67 | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NB500-170b | Biotin | AB_1660247 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/Ki-67_Biotin/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [O88713](https://www.uniprot.org/uniprotkb/O88713) | Primary Antibody | KLRG1 | Mouse | Syrian Hamster | IgG2 | 2F1 | [BD Biosciences](https://www.bdbiosciences.com/) | 561619 | AF488 | AB_10898017 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/KLRG1_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P18627](https://www.uniprot.org/uniprotkb/P18627) | Primary Antibody | LAG-3 | Human | Rabbit | IgG | Polyclonal | [LSBio](https://www.lsbio.com/) | LS-CS165666 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/LAG-3_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P18627](https://www.uniprot.org/uniprotkb/P18627) | Primary Antibody | LAG-3 | Human | Mouse | IgG1 | 17B4 | [Novus Biologicals](https://www.novusbio.com/) | NBP1-97657 | AF488 | AB_11162489 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/LAG-3_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [O88713](https://www.uniprot.org/uniprotkb/O88713) | Primary Antibody | KLRG1 | Mouse | Syrian Hamster | IgG2 | 2F1 | [BD Biosciences](https://www.bdbiosciences.com/) | 561619 | AF488 | AB_10898017 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/KLRG1_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [O88713](https://www.uniprot.org/uniprotkb/O88713) | Primary Antibody | KLRG1 | Mouse | Syrian Hamster | IgG2 | 2F1 | [BD Biosciences](https://www.bdbiosciences.com/) | 561619 | AF488 | AB_10898017 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/KLRG1_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P18627](https://www.uniprot.org/uniprotkb/P18627) | Primary Antibody | LAG-3 | Human | Mouse | IgG1 | 17B4 | [Novus Biologicals](https://www.novusbio.com/) | NBP1-97657IR | DL755 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/LAG-3_DL755/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P18627](https://www.uniprot.org/uniprotkb/P18627) | Primary Antibody | LAG-3 | Human | Mouse | IgG1 | 17B4 | [Novus Biologicals](https://www.novusbio.com/) | NBP1-97657 | AF488 | AB_11162489 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/LAG-3_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P18627](https://www.uniprot.org/uniprotkb/P18627) | Primary Antibody | LAG-3 | Human | Rabbit | IgG | Polyclonal | [LSBio](https://www.lsbio.com/) | LS-CS165666 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/LAG-3_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P02545](https://www.uniprot.org/uniprotkb/P02545) | Primary Antibody | Lamin A | Human | Mouse | IgG3 | 133A2 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MUB1101L1 | FITC | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Colon | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Lamin_A_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P11047](https://www.uniprot.org/uniprotkb/P11047) | Primary Antibody | Laminin | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NB300-144AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | [0000-0003-4379-8967](supporting_material/Laminin_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P02468](https://www.uniprot.org/uniprotkb/P02468), [P19137](https://www.uniprot.org/uniprotkb/P19137) | Primary Antibody | Laminin 1 + 2 | Mouse | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab7463 | Unconjugated | AB_305933 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | NA | Yes | [0000-0003-1431-1398](supporting_material/Laminin_1_+_2_Unconjugated/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | @@ -893,148 +899,148 @@ Additional information on fluorescent probes tested by the IBEX Imaging Communit | [P59901](https://www.uniprot.org/uniprotkb/P59901) | Primary Antibody | LILRA4 (CD85g) | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NBP2-15012 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/LILRA4__CD85g__Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [D0PNI2](https://www.uniprot.org/uniprotkb/D0PNI2) | Primary Antibody | LOX | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NB100-2530AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Tumor | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/LOX_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q12912](https://www.uniprot.org/uniprotkb/Q12912) | Primary Antibody | LRMP | Human | Rabbit | IgG | EPR17852 | [Abcam](https://www.abcam.com/) | ab202418 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/LRMP_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P51884](https://www.uniprot.org/uniprotkb/P51884) | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 | Unconjugated | AB_2139484 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-5187-810X](supporting_material/Lumican_Unconjugated/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | +| [P51884](https://www.uniprot.org/uniprotkb/P51884) | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 (Unconjugated) | AF680 | AB_2892760 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Lumican_AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P51884](https://www.uniprot.org/uniprotkb/P51884) | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 | Unconjugated | AB_2139484 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0002-2692-1225](supporting_material/Lumican_Unconjugated/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | +| [P51884](https://www.uniprot.org/uniprotkb/P51884) | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 (Unconjugated) | iF594 | AB_2892764 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Lumican_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P51884](https://www.uniprot.org/uniprotkb/P51884) | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 | Unconjugated | AB_2139484 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/Lumican_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [A0A8C0SV20](https://www.uniprot.org/uniprotkb/A0A8C0SV20) | Primary Antibody | Lumican | Canine | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 | Unconjugated | AB_2139484 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0001-5088-7808](supporting_material/Lumican_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | | [P51884](https://www.uniprot.org/uniprotkb/P51884) | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Lumican_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P51884](https://www.uniprot.org/uniprotkb/P51884) | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 | Unconjugated | AB_2139484 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Lumican_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P51884](https://www.uniprot.org/uniprotkb/P51884) | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 (Unconjugated) | iF594 | AB_2892764 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Lumican_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [A0A8C0SV20](https://www.uniprot.org/uniprotkb/A0A8C0SV20) | Primary Antibody | Lumican | Canine | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 | Unconjugated | AB_2139484 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/Lumican_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | -| [P51884](https://www.uniprot.org/uniprotkb/P51884) | Primary Antibody | Lumican | Human | Rabbit | IgG | EPR8898(2) | [Abcam](https://www.abcam.com/) | Ab252012 | PE | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/Lumican_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P51884](https://www.uniprot.org/uniprotkb/P51884) | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Lumican_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P51884](https://www.uniprot.org/uniprotkb/P51884) | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 | Unconjugated | AB_2139484 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/Lumican_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [A0A8C0SV20](https://www.uniprot.org/uniprotkb/A0A8C0SV20) | Primary Antibody | Lumican | Canine | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 | Unconjugated | AB_2139484 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/Lumican_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | | [P51884](https://www.uniprot.org/uniprotkb/P51884) | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 | Unconjugated | AB_2139484 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/Lumican_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P51884](https://www.uniprot.org/uniprotkb/P51884) | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 | Unconjugated | AB_2139484 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0002-2692-1225](supporting_material/Lumican_Unconjugated/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | -| [P51884](https://www.uniprot.org/uniprotkb/P51884) | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 | Unconjugated | AB_2139484 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-5187-810X](supporting_material/Lumican_Unconjugated/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | -| [A0A8C0SV20](https://www.uniprot.org/uniprotkb/A0A8C0SV20) | Primary Antibody | Lumican | Canine | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 | Unconjugated | AB_2139484 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0001-5088-7808](supporting_material/Lumican_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | -| [P51884](https://www.uniprot.org/uniprotkb/P51884) | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2846 (Unconjugated) | AF680 | AB_2892760 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Lumican_AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P51884](https://www.uniprot.org/uniprotkb/P51884) | Primary Antibody | Lumican | Human | Rabbit | IgG | EPR8898(2) | [Abcam](https://www.abcam.com/) | Ab252012 | PE | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/Lumican_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q8R2S8](https://www.uniprot.org/uniprotkb/Q8R2S8) | Primary Antibody | Ly-6G | Mouse | Rat | IgG2a | 1A8 | [BioLegend](https://www.biolegend.com/) | 127626 | AF488 | AB_2561340 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Ly-6G_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q6P698](https://www.uniprot.org/uniprotkb/Q6P698) | Primary Antibody | Lymphocyte cytosolic protein 1 (L-plastin/LCP1) | Zebrafish | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 13025-1-AP | Unconjugated | AB_2136871 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Lymphocyte_cytosolic_protein_1__L-plastin_LCP1__Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [Q6P698](https://www.uniprot.org/uniprotkb/Q6P698) | Primary Antibody | Lymphocyte cytosolic protein 1 (L-plastin/LCP1) | Zebrafish | Rabbit | IgG | Polyclonal | [GeneTex](https://www.genetex.com/) | GTX124420 | Unconjugated | AB_11167454 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Lymphocyte_cytosolic_protein_1__L-plastin_LCP1__Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [P61626](https://www.uniprot.org/uniprotkb/P61626) | Primary Antibody | Lysozyme | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab2408 | Unconjugated | AB_303050 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Lysozyme_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [A0A8P0NA08](https://www.uniprot.org/uniprotkb/A0A8P0NA08) | Primary Antibody | LYVE-1 | Canine | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2089 | Unconjugated | AB_355144 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0001-5088-7808](supporting_material/LYVE-1_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | +| [Q8BHC0](https://www.uniprot.org/uniprotkb/Q8BHC0) | Primary Antibody | LYVE-1 | Mouse | Rat | IgG1 | ALY7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-0443-82 | eF570 | AB_2573596 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/LYVE-1_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q8BHC0](https://www.uniprot.org/uniprotkb/Q8BHC0) | Primary Antibody | LYVE-1 | Mouse | Rat | IgG1 | ALY7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-0443-82 | eF570 | AB_2573596 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/LYVE-1_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9Y5Y7](https://www.uniprot.org/uniprotkb/Q9Y5Y7) | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2089 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/LYVE-1_iF594/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [Q9Y5Y7](https://www.uniprot.org/uniprotkb/Q9Y5Y7) | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2089 (Unconjugated) | AF532 | AB_2892756 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/LYVE-1_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q8BHC0](https://www.uniprot.org/uniprotkb/Q8BHC0) | Primary Antibody | LYVE-1 | Mouse | Rat | IgG1 | ALY7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-0443-82 | eF570 | AB_2573596 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/LYVE-1_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q8BHC0](https://www.uniprot.org/uniprotkb/Q8BHC0) | Primary Antibody | LYVE-1 | Mouse | Rat | IgG1 | ALY7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-0443-82 | eF570 | AB_2573596 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/LYVE-1_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [A0A8P0NA08](https://www.uniprot.org/uniprotkb/A0A8P0NA08) | Primary Antibody | LYVE-1 | Canine | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2089 | Unconjugated | AB_355144 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0001-5088-7808](supporting_material/LYVE-1_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | -| [Q9Y5Y7](https://www.uniprot.org/uniprotkb/Q9Y5Y7) | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2089 | Unconjugated | AB_355144 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/LYVE-1_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q8BHC0](https://www.uniprot.org/uniprotkb/Q8BHC0) | Primary Antibody | LYVE-1 | Mouse | Rabbit | IgG | E3L3V | [Cell Signaling Technology](https://www.cellsignal.com/) | 67538 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lymph Node | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | [0000-0003-0666-4804](supporting_material/LYVE-1_Unconjugated/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | -| [F7AVS7](https://www.uniprot.org/uniprotkb/F7AVS7) | Primary Antibody | LYVE-1 | Rhesus Macaque | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2089 | Unconjugated | AB_355144 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/LYVE-1_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [Q9Y5Y7](https://www.uniprot.org/uniprotkb/Q9Y5Y7) | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2089 (Unconjugated) | AF532 | AB_2892756 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/LYVE-1_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9Y5Y7](https://www.uniprot.org/uniprotkb/Q9Y5Y7) | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2089 | Unconjugated | AB_355144 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/LYVE-1_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9Y5Y7](https://www.uniprot.org/uniprotkb/Q9Y5Y7) | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2089 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/LYVE-1_iF594/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [Q8BHC0](https://www.uniprot.org/uniprotkb/Q8BHC0) | Primary Antibody | LYVE-1 | Mouse | Rabbit | IgG | E3L3V | [Cell Signaling Technology](https://www.cellsignal.com/) | 67538 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lymph Node | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | [0000-0003-0666-4804](supporting_material/LYVE-1_Unconjugated/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | | [Q9Y5Y7](https://www.uniprot.org/uniprotkb/Q9Y5Y7) | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2089 | Unconjugated | AB_355144 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/LYVE-1_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9Y5Y7](https://www.uniprot.org/uniprotkb/Q9Y5Y7) | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2089 | Unconjugated | AB_355144 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/LYVE-1_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q9Y5Y7](https://www.uniprot.org/uniprotkb/Q9Y5Y7) | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2089 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/LYVE-1_iF594/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [Q8BHC0](https://www.uniprot.org/uniprotkb/Q8BHC0) | Primary Antibody | LYVE-1 | Mouse | Rat | IgG1 | ALY7 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 41-0443-82 | eF570 | AB_2573596 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/LYVE-1_eF570/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9Y5Y7](https://www.uniprot.org/uniprotkb/Q9Y5Y7) | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2089 (Unconjugated) | AF532 | AB_2892756 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/LYVE-1_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9Y5Y7](https://www.uniprot.org/uniprotkb/Q9Y5Y7) | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2089 | Unconjugated | AB_355144 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/LYVE-1_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q9Y5Y7](https://www.uniprot.org/uniprotkb/Q9Y5Y7) | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2089 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | [0000-0001-9561-4256](supporting_material/LYVE-1_iF594/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [F7AVS7](https://www.uniprot.org/uniprotkb/F7AVS7) | Primary Antibody | LYVE-1 | Rhesus Macaque | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2089 | Unconjugated | AB_355144 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/LYVE-1_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [Q9UEW3](https://www.uniprot.org/uniprotkb/Q9UEW3) | Primary Antibody | MARCO | Human | Rabbit | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | PA5-64134 | Unconjugated | AB_2643767 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/MARCO_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | mCherrry | Mouse | Chicken | IgY | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NBP2-25158 | NA | AB_2636881 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-6944-6997](supporting_material/mCherrry_NA/0000-0002-6944-6997.md) | NA | 0000-0002-6944-6997 | | [A0A8C0S4Q3](https://www.uniprot.org/uniprotkb/A0A8C0S4Q3) | Primary Antibody | Melan-A | Canine | Mouse | IgG1 | rMLANA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 2315-MSM12-P1 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/Melan-A_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | | [Q13421](https://www.uniprot.org/uniprotkb/Q13421) | Primary Antibody | Mesothelin | Human | Rabbit | IgG | YP158 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-75255 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Peritoneum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/Mesothelin_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q13421](https://www.uniprot.org/uniprotkb/Q13421) | Primary Antibody | Mesothelin | Human | Mouse | IgG2a | MB-G10 | [Novus Biologicals](https://www.novusbio.com/) | NB110-85538 | Unconjugated | AB_1199375 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Peritoneum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/Mesothelin_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [BioLegend](https://www.biolegend.com/) | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/MHC-II_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [BioLegend](https://www.biolegend.com/) | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/MHC-II_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [BioLegend](https://www.biolegend.com/) | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/MHC-II_AF700/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | -| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [BioLegend](https://www.biolegend.com/) | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/MHC-II_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [BioLegend](https://www.biolegend.com/) | 107618 | AF647 | AB_493525 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/MHC-II_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [BioLegend](https://www.biolegend.com/) | 107620 | Pacific Blue | AB_493527 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/MHC-II_Pacific_Blue/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | -| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 48-5321-82 | eF450 | AB_1272204 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/MHC-II_eF450/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | -| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [BioLegend](https://www.biolegend.com/) | 107631 | BV421 | AB_10900075 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/MHC-II_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [BioLegend](https://www.biolegend.com/) | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/MHC-II_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11002 | AF532 | AB_2534070 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-10038 | AF680 | AB_2534014 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11031 | AF568 | AB_144696 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF568/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Mouse IgG (H+L) | Canine | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21203 | AF594 | AB_141633 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0001-5088-7808](supporting_material/Mouse_IgG__H+L__AF594/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | -| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | [AAT Bioquest](https://www.aatbio.com/) | 16548 | iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21052 | AF633 | AB_2535719 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF633/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21236 | AF647 | AB_2535805 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [BioLegend](https://www.biolegend.com/) | 107620 | Pacific Blue | AB_493527 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/MHC-II_Pacific_Blue/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | +| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [BioLegend](https://www.biolegend.com/) | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/MHC-II_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 48-5321-82 | eF450 | AB_1272204 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/MHC-II_eF450/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | +| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [BioLegend](https://www.biolegend.com/) | 107631 | BV421 | AB_10900075 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/MHC-II_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [BioLegend](https://www.biolegend.com/) | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/MHC-II_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [BioLegend](https://www.biolegend.com/) | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/MHC-II_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [BioLegend](https://www.biolegend.com/) | 107618 | AF647 | AB_493525 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/MHC-II_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [BioLegend](https://www.biolegend.com/) | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/MHC-II_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | [BioLegend](https://www.biolegend.com/) | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/MHC-II_AF700/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | +| NA | Secondary Antibody | Mouse IgG (H+L) | Zebrafish | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21235 | AF647 | AB_2535804 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Mouse_IgG__H+L__AF647/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21036 | AF700 | AB_2535707 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | SA5-10175 | DL755 | AB_2556755 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__DL755/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21203 | AF594 | AB_141633 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21236 | AF647 | AB_2535805 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21052 | AF633 | AB_2535719 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF633/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | [AAT Bioquest](https://www.aatbio.com/) | 16548 | iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11030 | AF546 | AB_2534089 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF546/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21202 | AF488 | AB_141607 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11031 | AF568 | AB_144696 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF568/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11002 | AF532 | AB_2534070 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-10038 | AF680 | AB_2534014 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | PA1-28626 | Texas Red | AB_10983944 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__Texas_Red/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21202 | AF488 | AB_141607 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | SA5-10175 | DL755 | AB_2556755 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__DL755/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-31570 | AF555 | AB_2536180 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Mouse IgG (H+L) | Rhesus Macaque | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-31570 | AF555 | AB_2536180 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | [0009-0004-2746-8743](supporting_material/Mouse_IgG__H+L__AF555/0009-0004-2746-8743.md) | NA | 0009-0004-2746-8743 | -| NA | Secondary Antibody | Mouse IgG (H+L) | Zebrafish | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21235 | AF647 | AB_2535804 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Mouse_IgG__H+L__AF647/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | +| NA | Secondary Antibody | Mouse IgG (H+L) | Canine | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21203 | AF594 | AB_141633 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0001-5088-7808](supporting_material/Mouse_IgG__H+L__AF594/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | | NA | Secondary Antibody | Mouse IgG (H+L) | Zebrafish | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11030 | AF546 | AB_2737024 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Mouse_IgG__H+L__AF546/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | +| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21203 | AF594 | AB_141633 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG__H+L__AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Mouse IgG1 | Human | Alpaca | IgG1 | CTK0103/CTK0104 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | SA5-10331 | AF568 | AB_2868378 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG1_AF568/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Mouse IgG1 | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21121 | AF488 | AB_2535764 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG1_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Mouse IgG1 | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21121 | AF488 | AB_2535764 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG1_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Zenon Labeling Kit | Mouse IgG1 | Human | NA | Fab2 | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | Z25011 | AF700 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG1_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Mouse IgG2a | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21137 | AF555 | AB_2535776 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG2a_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Mouse IgG2a | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21137 | AF555 | AB_2535776 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG2a_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Mouse IgG2a | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21137 | AF555 | AB_2535776 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG2a_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Mouse IgG2b | Human | Alpaca | IgG2b | CTK0105/CTK0106 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | SA5-10339 | AF647 | AB_2868386 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgG2b_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 715-545-020 | AF488 | AB_2340844 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgM_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 715-545-020 | AF488 | AB_2340844 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgM_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 715-605-020 | AF647 | AB_2340860 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgM_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 715-605-020 | AF647 | AB_2340860 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgM_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 715-095-140 | FITC | AB_2340791 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgM_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 715-605-020 | AF647 | AB_2340860 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgM_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 715-545-020 | AF488 | AB_2340844 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgM_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 715-545-020 | AF488 | AB_2340844 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgM_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 715-545-020 | AF488 | AB_2340844 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Mouse_IgM_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | Mycobacterium tuberculosis | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab905 | Unconjugated | AB_306965 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-3697-5726](supporting_material/Mycobacterium_tuberculosis_Unconjugated/0000-0003-3697-5726.md) | NA | 0000-0003-3697-5726 | | NA | Primary Antibody | Mycobacterium tuberculosis Ag85B | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab43019 | Unconjugated | AB_776575 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-3697-5726](supporting_material/Mycobacterium_tuberculosis_Ag85B_Unconjugated/0000-0003-3697-5726.md) | NA | 0000-0003-3697-5726 | +| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | S11224 | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/NA_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | [AAT Bioquest](https://www.aatbio.com/) | 16956 | iF514 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/NA_iF514/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 016-620-084 | AF680 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/NA_AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 48-4317-82 | eF450 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/NA_eF450/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | [BioLegend](https://www.biolegend.com/) | 405235 | AF488 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/NA_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | FlexAble CoraLite Plus 750 Antibody Labeling Kit for Rabbit IgG | NA | Zebrafish | NA | NA | NA | [Proteintech](https://www.ptglab.com/) | KFA004 | CoraLite Plus AF750 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | [0009-0000-2047-4228](supporting_material/NA_CoraLite_Plus_AF750/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | +| NA | FlexAble CoraLite Plus 550 Antibody Labeling Kit for Rabbit IgG | NA | Zebrafish | NA | NA | NA | [Proteintech](https://www.ptglab.com/) | KFA002 | CoraLite Plus AF550 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | [0009-0000-2047-4228](supporting_material/NA_CoraLite_Plus_AF550/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | +| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | [BioLegend](https://www.biolegend.com/) | 405203 | PE | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/NA_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | FlexAble CoraLite Plus 488 Antibody Labeling Kit for Rabbit IgG | NA | Zebrafish | NA | NA | NA | [Proteintech](https://www.ptglab.com/) | KFA001 | CoraLite Plus AF488 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | [0009-0000-2047-4228](supporting_material/NA_CoraLite_Plus_AF488/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | S21384 | AF750 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/NA_AF750/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | [AAT Bioquest](https://www.aatbio.com/) | 16956 | iF514 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/NA_iF514/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | FlexAble CoraLite Plus 750 Antibody Labeling Kit for Rabbit IgG | NA | Zebrafish | NA | NA | NA | [Proteintech](https://www.ptglab.com/) | KFA004 | CoraLite Plus AF750 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | [0009-0000-2047-4228](supporting_material/NA_CoraLite_Plus_AF750/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | +| NA | Avidin/Biotin Blocking Kit | NA | Human | NA | NA | NA | [Abcam](https://www.abcam.com/) | ab64212 | NA | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/NA_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Avidin/Biotin Blocking Kit | NA | Human | NA | NA | NA | [Abcam](https://www.abcam.com/) | ab64212 | NA | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/NA_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | S21384 | AF750 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/NA_AF750/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 48-4317-82 | eF450 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/NA_eF450/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | S21384 | AF750 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/NA_AF750/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | FlexAble CoraLite Plus 555 Antibody Labeling Kit for Mouse IgG1 | NA | Zebrafish | NA | NA | NA | [Proteintech](https://www.ptglab.com/) | KFA022 | CoraLite Plus AF555 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | [0009-0000-2047-4228](supporting_material/NA_CoraLite_Plus_AF555/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | S11224 | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/NA_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | FlexAble CoraLite Plus 647 Antibody Labeling Kit for Mouse IgG1 | NA | Zebrafish | NA | NA | NA | [Proteintech](https://www.ptglab.com/) | KFA023 | CoraLite Plus AF647 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | [0009-0000-2047-4228](supporting_material/NA_CoraLite_Plus_AF647/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| NA | Avidin/Biotin Blocking Kit | NA | Human | NA | NA | NA | [Abcam](https://www.abcam.com/) | ab64212 | NA | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/NA_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Avidin/Biotin Blocking Kit | NA | Human | NA | NA | NA | [Abcam](https://www.abcam.com/) | ab64212 | NA | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/NA_NA/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | FlexAble CoraLite Plus 550 Antibody Labeling Kit for Rabbit IgG | NA | Zebrafish | NA | NA | NA | [Proteintech](https://www.ptglab.com/) | KFA002 | CoraLite Plus AF550 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | [0009-0000-2047-4228](supporting_material/NA_CoraLite_Plus_AF550/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | [BioLegend](https://www.biolegend.com/) | 405203 | PE | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/NA_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 016-620-084 | AF680 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/NA_AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q13562](https://www.uniprot.org/uniprotkb/Q13562) | Primary Antibody | NeuroD1 | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NBP2-98697AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-2950-2683](supporting_material/NeuroD1_AF647/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | | [P08246](https://www.uniprot.org/uniprotkb/P08246) | Primary Antibody | Neutrophil elastase | Human | Sheep | IgG | Polyclonal | [LSBio](https://www.lsbio.com/) | LS-B4244-50 | Unconjugated | AB_10912151 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-3882-457X](supporting_material/Neutrophil_elastase_Unconjugated/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | | [P12036](https://www.uniprot.org/uniprotkb/P12036) | Primary Antibody | NF-H | Human | Mouse | IgG1 | SMI32 | [BioLegend](https://www.biolegend.com/) | 801705 | AF488 | AB_2715876 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/NF-H_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | NF-H/NF-M | Human | Mouse | IgG1 | SMI310 | [BioLegend](https://www.biolegend.com/) | 837711 | AF488 | AB_2750293 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/NF-H_NF-M_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P07196](https://www.uniprot.org/uniprotkb/P07196) | Primary Antibody | NF-L | Human | Mouse | IgG1 | NFL3 | [BioLegend](https://www.biolegend.com/) | 845907 | AF647 | AB_2728564 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/NF-L_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P30548](https://www.uniprot.org/uniprotkb/P30548) | Primary Antibody | NK1.1 | Mouse | Mouse | IgG2a | PK136 | [BioLegend](https://www.biolegend.com/) | 108731 | BV421 | AB_10895916 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-1431-1398](supporting_material/NK1.1_BV421/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | | [P30548](https://www.uniprot.org/uniprotkb/P30548) | Primary Antibody | NK1.1 | Mouse | Mouse | IgG2a | PK136 | [BioLegend](https://www.biolegend.com/) | 108731 | BV421 | AB_10895916 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/NK1.1_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P30548](https://www.uniprot.org/uniprotkb/P30548) | Primary Antibody | NK1.1 | Mouse | Mouse | IgG2a | PK136 | [BioLegend](https://www.biolegend.com/) | 108731 | BV421 | AB_10895916 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-1431-1398](supporting_material/NK1.1_BV421/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | | [Q8C567](https://www.uniprot.org/uniprotkb/Q8C567) | Primary Antibody | NKp46 | Mouse | Rat | IgG2a | 29A1.4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 12-3351-82 | PE | AB_1210743 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-7422-3237](supporting_material/NKp46_PE/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | | [Q92823](https://www.uniprot.org/uniprotkb/Q92823) | Primary Antibody | NrCAM | Human | Mouse | IgG2a | N343/26 | [BioLegend](https://www.biolegend.com/) | 821602 | Unconjugated | AB_2734588 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-3882-457X](supporting_material/NrCAM_Unconjugated/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | | NA | Primary Antibody | Nuclear Pore Complex | Mouse | Mouse | IgG1 | MAb414 | [BioLegend](https://www.biolegend.com/) | 682202 | AF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0002-5187-810X](supporting_material/Nuclear_Pore_Complex_AF594/0000-0002-5187-810X.md) | NA | 0000-0002-5187-810X | -| [P22736](https://www.uniprot.org/uniprotkb/P22736) | Primary Antibody | Nur77 | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab48789 | Unconjugated | AB_881709 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/Nur77_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P22736](https://www.uniprot.org/uniprotkb/P22736) | Primary Antibody | Nur77 | Human | Mouse | IgG2b | 1E10A15 | [BioLegend](https://www.biolegend.com/) | 653504 | AF594 | AB_2563321 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/Nur77_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P22736](https://www.uniprot.org/uniprotkb/P22736) | Primary Antibody | Nur77 | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab48789 | Unconjugated | AB_881709 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/Nur77_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P51491](https://www.uniprot.org/uniprotkb/P51491) | Primary Antibody | Opsin Blue | Mouse | Rabbit | IgG | Polyclonal | [MilliporeSigma](https://www.sigmaaldrich.com/) | AB5407 | Unconjugated | AB_177457 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | [0000-0003-2088-8310](supporting_material/Opsin_Blue_Unconjugated/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [P03999](https://www.uniprot.org/uniprotkb/P03999) | Primary Antibody | Opsin Blue | Human | Rabbit | IgG | Polyclonal | [MilliporeSigma](https://www.sigmaaldrich.com/) | AB5407 | Unconjugated | AB_177457 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | [0000-0003-2088-8310](supporting_material/Opsin_Blue_Unconjugated/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | -| [P04000](https://www.uniprot.org/uniprotkb/P04000) | Primary Antibody | Opsin Red/Green | Human | Rabbit | IgG | Polyclonal | [MilliporeSigma](https://www.sigmaaldrich.com/) | AB5405 | Unconjugated | AB_177456 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | [0000-0003-2088-8310](supporting_material/Opsin_Red_Green_Unconjugated/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | Q9W6A7/Q9W6A5 | Primary Antibody | Opsin Red/Green | Zebrafish | Rabbit | IgG | Polyclonal | [MilliporeSigma](https://www.sigmaaldrich.com/) | AB5405 | Unconjugated | AB_177456 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Opsin_Red_Green_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [P04000](https://www.uniprot.org/uniprotkb/P04000) | Primary Antibody | Opsin Red/Green | Mouse | Rabbit | IgG | Polyclonal | [MilliporeSigma](https://www.sigmaaldrich.com/) | AB5405 | Unconjugated | AB_177456 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | [0000-0003-2088-8310](supporting_material/Opsin_Red_Green_Unconjugated/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | +| [P04000](https://www.uniprot.org/uniprotkb/P04000) | Primary Antibody | Opsin Red/Green | Human | Rabbit | IgG | Polyclonal | [MilliporeSigma](https://www.sigmaaldrich.com/) | AB5405 | Unconjugated | AB_177456 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | [0000-0003-2088-8310](supporting_material/Opsin_Red_Green_Unconjugated/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [P15863](https://www.uniprot.org/uniprotkb/P15863) | Primary Antibody | PAX1 | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab203065 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-3882-457X](supporting_material/PAX1_Unconjugated/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | | [Q02650](https://www.uniprot.org/uniprotkb/Q02650) | Primary Antibody | Pax5 | Mouse | Rat | IgG2a | 1H9 | [BioLegend](https://www.biolegend.com/) | 649704 | AF647 | AB_2562425 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Pax5_AF647/0000-0003-4379-8967.md), [0000-0002-1461-0999](supporting_material/Pax5_AF647/0000-0002-1461-0999.md) | NA | 0000-0003-4379-8967 | | [A0A8C6NQH8](https://www.uniprot.org/uniprotkb/A0A8C6NQH8) | Primary Antibody | PAX6 | Turquoise Killifish | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 12323-1-AP | Unconjugated | AB_2159695 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/PAX6_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [P55864](https://www.uniprot.org/uniprotkb/P55864) | Primary Antibody | PAX6 | African Clawed Frog (Xenopus laevis) | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 12323-1-AP | Unconjugated | AB_2159695 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/PAX6_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [P26630](https://www.uniprot.org/uniprotkb/P26630) | Primary Antibody | PAX6 | Zebrafish | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 12323-1-AP | Unconjugated | AB_2159695 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/PAX6_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| [P18248](https://www.uniprot.org/uniprotkb/P18248) | Primary Antibody | PCNA | African Clawed Frog (Xenopus laevis) | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 24036-1-AP | Unconjugated | AB_2879414 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/PCNA_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| [Q9PTP1](https://www.uniprot.org/uniprotkb/Q9PTP1) | Primary Antibody | PCNA | Zebrafish | Mouse | IgG2a | PC10 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-56 | Unconjugated | AB_628110 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/PCNA_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| [Q9PTP1](https://www.uniprot.org/uniprotkb/Q9PTP1) | Primary Antibody | PCNA | Zebrafish | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 24036-1-AP | Unconjugated | AB_2879414 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/PCNA_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | NA | Primary Antibody | PCNA | Turquoise Killifish | Mouse | IgG2a | PC10 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-56 | Unconjugated | AB_628110 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/PCNA_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | +| [Q9PTP1](https://www.uniprot.org/uniprotkb/Q9PTP1) | Primary Antibody | PCNA | Zebrafish | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 24036-1-AP | Unconjugated | AB_2879414 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/PCNA_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | +| [P18248](https://www.uniprot.org/uniprotkb/P18248) | Primary Antibody | PCNA | African Clawed Frog (Xenopus laevis) | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 24036-1-AP | Unconjugated | AB_2879414 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/PCNA_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [P18248](https://www.uniprot.org/uniprotkb/P18248) | Primary Antibody | PCNA | African Clawed Frog (Xenopus laevis) | Mouse | IgG2a | PC10 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-56 | Unconjugated | AB_628110 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/PCNA_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | NA | Primary Antibody | PCNA | Turquoise Killifish | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 24036-1-AP | Unconjugated | AB_2879414 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/PCNA_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | +| [Q9PTP1](https://www.uniprot.org/uniprotkb/Q9PTP1) | Primary Antibody | PCNA | Zebrafish | Mouse | IgG2a | PC10 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-56 | Unconjugated | AB_628110 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/PCNA_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [P48539](https://www.uniprot.org/uniprotkb/P48539) | Primary Antibody | PCP4 | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NBP1-80929 | Unconjugated | AB_11026568 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](supporting_material/PCP4_Unconjugated/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | -| [Q02242](https://www.uniprot.org/uniprotkb/Q02242) | Primary Antibody | PD-1 | Mouse | Rat | IgG2a | 29F.1.A12 | [BioLegend](https://www.biolegend.com/) | 135206 | PE | AB_1877231 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/PD-1_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Rabbit | IgG | EPR4877(2) | [Abcam](https://www.abcam.com/) | ab275126 | AF555 | AB_2728811 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0009-0006-9784-2694](supporting_material/PD-1_AF555/0009-0006-9784-2694.md) | NA | 0009-0006-9784-2694 | -| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | [BioLegend](https://www.biolegend.com/) | 329906 | PE | AB_940483 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/PD-1_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | PD-1 | Rhesus Macaque | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | BAF1086 | Biotin | AB_355833 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/PD-1_Biotin/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [Q02242](https://www.uniprot.org/uniprotkb/Q02242) | Primary Antibody | PD-1 | Mouse | Rat | IgG2a | 29F.1.A12 | [BioLegend](https://www.biolegend.com/) | 135218 | BV421 | AB_2561447 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/PD-1_BV421/0000-0003-4379-8967.md), [0000-0003-1118-7432](supporting_material/PD-1_BV421/0000-0003-1118-7432.md) | NA | 0000-0003-4379-8967 | -| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | [BioLegend](https://www.biolegend.com/) | 329936 | AF488 | AB_2563594 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/PD-1_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | [BioLegend](https://www.biolegend.com/) | 329906 | PE | AB_940483 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/PD-1_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | [BioLegend](https://www.biolegend.com/) | 329906 | PE | AB_940483 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/PD-1_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | [BioLegend](https://www.biolegend.com/) | 329906 | PE | AB_940483 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/PD-1_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | [BioLegend](https://www.biolegend.com/) | 329920 | BV421 | AB_10960742 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/PD-1_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Rabbit | IgG | EPR4877(2) | [Abcam](https://www.abcam.com/) | ab275126 | AF555 | AB_2728811 | Stock | Multiplexed 2D Imaging | FFPE | Lymph node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/PD-1_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q02242](https://www.uniprot.org/uniprotkb/Q02242) | Primary Antibody | PD-1 | Mouse | Rat | IgG2a | 29F.1.A12 | [BioLegend](https://www.biolegend.com/) | 135218 | BV421 | AB_2561447 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/PD-1_BV421/0000-0003-4379-8967.md), [0000-0003-1118-7432](supporting_material/PD-1_BV421/0000-0003-1118-7432.md) | NA | 0000-0003-4379-8967 | +| [A0A024FCJ9](https://www.uniprot.org/uniprotkb/A0A024FCJ9) | Primary Antibody | PD-1 | Canine | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF1086 | Unconjugated | AB_354588 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/PD-1_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | | NA | Primary Antibody | PD-1 | Rhesus Macaque | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | FAB7115G | AF488 | AB_10973668 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/PD-1_AF488/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| NA | Primary Antibody | PD-1 | Rhesus Macaque | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | BAF1086 | Biotin | AB_355833 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/PD-1_Biotin/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | +| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Rabbit | IgG | EPR4877(2) | [Abcam](https://www.abcam.com/) | ab275126 | AF555 | AB_2728811 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/PD-1_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | [BioLegend](https://www.biolegend.com/) | 329906 | PE | AB_940483 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/PD-1_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF1086 | Unconjugated | AB_354588 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/PD-1_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | | [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF1086 | Unconjugated | AB_354588 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/PD-1_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF1086 | Unconjugated | AB_354588 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/PD-1_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | [BioLegend](https://www.biolegend.com/) | 329906 | PE | AB_940483 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/PD-1_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q02242](https://www.uniprot.org/uniprotkb/Q02242) | Primary Antibody | PD-1 | Mouse | Rat | IgG2a | 29F.1.A12 | [BioLegend](https://www.biolegend.com/) | 135206 | PE | AB_1877231 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/PD-1_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | [BioLegend](https://www.biolegend.com/) | 329910 | AF647 | AB_940471 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/PD-1_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | [BioLegend](https://www.biolegend.com/) | 329906 | PE | AB_940483 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/PD-1_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | [BioLegend](https://www.biolegend.com/) | 329920 | BV421 | AB_10960742 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/PD-1_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Rabbit | IgG | EPR4877(2) | [Abcam](https://www.abcam.com/) | ab275126 | AF555 | AB_2728811 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0009-0006-9784-2694](supporting_material/PD-1_AF555/0009-0006-9784-2694.md) | NA | 0009-0006-9784-2694 | +| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | [BioLegend](https://www.biolegend.com/) | 329936 | AF488 | AB_2563594 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/PD-1_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF1086 | Unconjugated | AB_354588 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/PD-1_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF1086 | Unconjugated | AB_354588 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | [0000-0001-9561-4256](supporting_material/PD-1_Unconjugated/0000-0001-9561-4256.md) | NA | 0000-0001-9561-4256 | -| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF1086 | Unconjugated | AB_354588 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/PD-1_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q15116](https://www.uniprot.org/uniprotkb/Q15116) | Primary Antibody | PD-1 | Human | Rabbit | IgG | EPR4877(2) | [Abcam](https://www.abcam.com/) | ab275126 | AF555 | AB_2728811 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/PD-1_AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [A0A024FCJ9](https://www.uniprot.org/uniprotkb/A0A024FCJ9) | Primary Antibody | PD-1 | Canine | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF1086 | Unconjugated | AB_354588 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/PD-1_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | +| [A0A8C6KR86](https://www.uniprot.org/uniprotkb/A0A8C6KR86) | Primary Antibody | Peripherin 2 | Turquoise Killifish | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 18109-1-AP | Unconjugated | AB_10665364 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Peripherin_2_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [Q66HW7](https://www.uniprot.org/uniprotkb/Q66HW7) | Primary Antibody | Peripherin 2 | Zebrafish | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 18109-1-AP | Unconjugated | AB_10665364 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Peripherin_2_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [O42583](https://www.uniprot.org/uniprotkb/O42583) | Primary Antibody | Peripherin 2 | African Clawed Frog (Xenopus laevis) | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 18109-1-AP | Unconjugated | AB_10665364 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Peripherin_2_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| [A0A8C6KR86](https://www.uniprot.org/uniprotkb/A0A8C6KR86) | Primary Antibody | Peripherin 2 | Turquoise Killifish | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 18109-1-AP | Unconjugated | AB_10665364 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Peripherin_2_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [P60709](https://www.uniprot.org/uniprotkb/P60709) | Cell Structure Reagent | Phalloidin | Human | NA | NA | NA | [AAT Bioquest](https://www.aatbio.com/) | 23122 | iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/Phalloidin_iF594/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [P19838](https://www.uniprot.org/uniprotkb/P19838) | Primary Antibody | Phospho NF-Kappa B p105 | Human | Rabbit | IgG | 178F3 | [Cell Signaling Technology](https://www.cellsignal.com/) | PA5-79525 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/Phospho_NF-Kappa_B_p105_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P31751](https://www.uniprot.org/uniprotkb/P31751) | Primary Antibody | Phospho-Akt | Human | Rabbit | IgG | D9E | [Cell Signaling Technology](https://www.cellsignal.com/) | 4060T | Unconjugated | AB_2315049 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/Phospho-Akt_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | @@ -1045,8 +1051,8 @@ Additional information on fluorescent probes tested by the IBEX Imaging Communit | [P01833](https://www.uniprot.org/uniprotkb/P01833) | Primary Antibody | PIGR | Human | Rat | IgG1 | 7C1 | [Abcam](https://www.abcam.com/) | ab170321 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | [0000-0002-2950-2683](supporting_material/PIGR_Unconjugated/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | | [P01833](https://www.uniprot.org/uniprotkb/P01833) | Primary Antibody | PIGR | Human | Rat | IgG1 | 7C1 | [Abcam](https://www.abcam.com/) | ab170321 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](supporting_material/PIGR_Unconjugated/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | | [P01833](https://www.uniprot.org/uniprotkb/P01833) | Primary Antibody | PIGR | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab96196 | Unconjugated | AB_10677612 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](supporting_material/PIGR_Unconjugated/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | -| [P17252](https://www.uniprot.org/uniprotkb/P17252) | Primary Antibody | PKC alpha | Human | Mouse | IgG1 | H-7 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-8393 AF680 | AF680 | AB_628142 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/PKC_alpha_AF680/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [P20444](https://www.uniprot.org/uniprotkb/P20444) | Primary Antibody | PKC alpha | Mouse | Mouse | IgG1 | H-7 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-8393 AF680 | AF680 | AB_628142 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/PKC_alpha_AF680/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | +| [P17252](https://www.uniprot.org/uniprotkb/P17252) | Primary Antibody | PKC alpha | Human | Mouse | IgG1 | H-7 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-8393 AF680 | AF680 | AB_628142 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/PKC_alpha_AF680/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | NA | Lectin | PNA | Zebrafish | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | L21409 | AF488 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | [0009-0000-2047-4228](supporting_material/PNA_AF488/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | NA | Lectin | PNA | Human | NA | NA | NA | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | L21409 | AF488 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/PNA_AF488/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | NA | Primary Antibody | PNL-2 | Canine | Mouse | IgG1 | PNL2 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | MSM1-894-P0 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0001-5088-7808](supporting_material/PNL-2_Unconjugated/0000-0001-5088-7808.md) | NA | 0000-0001-5088-7808 | @@ -1055,75 +1061,75 @@ Additional information on fluorescent probes tested by the IBEX Imaging Communit | [Q9UKI9](https://www.uniprot.org/uniprotkb/Q9UKI9) | Primary Antibody | POU2F3 | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NBP1-83966 | Unconjugated | AB_11024500 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](supporting_material/POU2F3_Unconjugated/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | | [P02545](https://www.uniprot.org/uniprotkb/P02545) | Primary Antibody | Progerin | Human | Mouse | IgG1 | 13A4 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 39965 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](supporting_material/Progerin_Unconjugated/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | | [Q7SY24](https://www.uniprot.org/uniprotkb/Q7SY24) | Primary Antibody | Protein kinase C beta | Zebrafish | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 12919-1-AP | Unconjugated | AB_2237469 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Protein_kinase_C_beta_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| [P42225](https://www.uniprot.org/uniprotkb/P42225) | Primary Antibody | pSTAT1 | Mouse | Rabbit | IgG | 58D6 | [Cell Signaling Technology](https://www.cellsignal.com/) | 9167 | Unconjugated | NA | Stock | IBEX2D Manual | 2% PFA Fixed Frozen | Lung | Infected | Akoya Antibody Diluent | NA | NA | Yes | [0000-0003-0666-4804](supporting_material/pSTAT1_Unconjugated/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | -| [P42225](https://www.uniprot.org/uniprotkb/P42225) | Primary Antibody | pSTAT1 | Mouse | Rabbit | IgG | 58D6 | [Cell Signaling Technology](https://www.cellsignal.com/) | 9167 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lymph Node | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | [0000-0003-0666-4804](supporting_material/pSTAT1_Unconjugated/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | | [P42225](https://www.uniprot.org/uniprotkb/P42225) | Primary Antibody | pSTAT1 | Mouse | Rabbit | IgG | 58D6 | [Cell Signaling Technology](https://www.cellsignal.com/) | 9167 | Unconjugated | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | Akoya Antibody Diluent | NA | NA | Yes | [0000-0003-0666-4804](supporting_material/pSTAT1_Unconjugated/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | | [P42225](https://www.uniprot.org/uniprotkb/P42225) | Primary Antibody | pSTAT1 | Mouse | Rabbit | IgG | 58D6 | [Cell Signaling Technology](https://www.cellsignal.com/) | 9167 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | [0000-0003-0666-4804](supporting_material/pSTAT1_Unconjugated/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | -| [P42229](https://www.uniprot.org/uniprotkb/P42229) | Primary Antibody | pSTAT5 (Tyr694) | Human | Rabbit | IgG | C11C5 | [Cell Signaling Technology](https://www.cellsignal.com/) | 9359 | Unconjugated | AB_823649 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/pSTAT5__Tyr694__Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P42229](https://www.uniprot.org/uniprotkb/P42229) | Primary Antibody | pSTAT5 (Tyr694) | Human | Rabbit | IgG | C71E5 | [Cell Signaling Technology](https://www.cellsignal.com/) | 9314 | Unconjugated | AB_2302702 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/pSTAT5__Tyr694__Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P42225](https://www.uniprot.org/uniprotkb/P42225) | Primary Antibody | pSTAT1 | Mouse | Rabbit | IgG | 58D6 | [Cell Signaling Technology](https://www.cellsignal.com/) | 9167 | Unconjugated | NA | Stock | IBEX2D Manual | 2% PFA Fixed Frozen | Lung | Infected | Akoya Antibody Diluent | NA | NA | Yes | [0000-0003-0666-4804](supporting_material/pSTAT1_Unconjugated/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | +| [P42225](https://www.uniprot.org/uniprotkb/P42225) | Primary Antibody | pSTAT1 | Mouse | Rabbit | IgG | 58D6 | [Cell Signaling Technology](https://www.cellsignal.com/) | 9167 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lymph Node | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | [0000-0003-0666-4804](supporting_material/pSTAT1_Unconjugated/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | | [P42229](https://www.uniprot.org/uniprotkb/P42229) | Primary Antibody | pSTAT5 (Tyr694) | Human | Rabbit | IgG | D47E7 | [Cell Signaling Technology](https://www.cellsignal.com/) | 4322 | Unconjugated | AB_10544692 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/pSTAT5__Tyr694__Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21206 | AF488 | AB_2535792 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P42229](https://www.uniprot.org/uniprotkb/P42229) | Primary Antibody | pSTAT5 (Tyr694) | Human | Rabbit | IgG | C71E5 | [Cell Signaling Technology](https://www.cellsignal.com/) | 9314 | Unconjugated | AB_2302702 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/pSTAT5__Tyr694__Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P42229](https://www.uniprot.org/uniprotkb/P42229) | Primary Antibody | pSTAT5 (Tyr694) | Human | Rabbit | IgG | C11C5 | [Cell Signaling Technology](https://www.cellsignal.com/) | 9359 | Unconjugated | AB_823649 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/pSTAT5__Tyr694__Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Human | Goat | Fab2 | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | Z25305 | AF555 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-3882-457X](supporting_material/Rabbit_IgG__H+L__AF555/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-31572 | AF555 | AB_162543 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-31572 | AF555 | AB_162543 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Human | Goat | Fab2 | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | Z25308 | AF647 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11009 | AF532 | AB_2534076 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1118-7432](supporting_material/Rabbit_IgG__H+L__AF532/0000-0003-1118-7432.md) | NA | 0000-0003-1118-7432 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Zebrafish | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21245 | AF647 | AB_2535813 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Rabbit_IgG__H+L__AF647/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Zebrafish | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-10042 | AF546 | AB_2534017 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Rabbit_IgG__H+L__AF546/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | +| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Mouse | Goat | Fab2 | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | Z25307 | AF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-1431-1398](supporting_material/Rabbit_IgG__H+L__AF594/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | | NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11034 | AF488 | AB_2576217 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Human | Goat | Fab2 | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | Z25302 | AF488 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21038 | AF700 | AB_2535709 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [AAT Bioquest](https://www.aatbio.com/) | 16712 | iF680 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__iF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [AAT Bioquest](https://www.aatbio.com/) | 16710 | iF647 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__iF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21109 | AF680 | AB_2535758 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21206 | AF488 | AB_2535792 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Human | Goat | Fab2 | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | Z25303 | AF532 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [AAT Bioquest](https://www.aatbio.com/) | 16690 | iF555 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__iF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11009 | AF532 | AB_2534076 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-10040 | AF546 | AB_2534016 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF546/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [AAT Bioquest](https://www.aatbio.com/) | 16686 | iF532 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__iF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11010 | AF546 | AB_2534077 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF546/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [MilliporeSigma](https://www.sigmaaldrich.com/) | SAB4600110 | CF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__CF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [AAT Bioquest](https://www.aatbio.com/) | 16678 | iF488 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__iF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-31572 | AF555 | AB_162543 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21207 | AF594 | AB_141637 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11009 | AF532 | AB_2534076 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1118-7432](supporting_material/Rabbit_IgG__H+L__AF532/0000-0003-1118-7432.md) | NA | 0000-0003-1118-7432 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [Vector Laboratories](https://vectorlabs.com/) | TI-10000 | Texas Red | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__Texas_Red/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab155728 | AF750 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF750/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21074 | AF660 | AB_2535735 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21109 | AF680 | AB_2535758 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | CL594-66467 | CL594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__CL594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [Vector Laboratories](https://vectorlabs.com/) | TI-10000 | Texas Red | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__Texas_Red/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [MilliporeSigma](https://www.sigmaaldrich.com/) | SAB4600110 | CF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__CF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11010 | AF546 | AB_2534077 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF546/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11374 | AF790 | AB_2534145 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF790/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-31573 | AF647 | AB_2536183 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-10040 | AF546 | AB_2534016 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF546/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21207 | AF594 | AB_141637 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-10043 | AF680 | AB_2534018 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-31572 | AF555 | AB_162543 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Zebrafish | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-10042 | AF546 | AB_2534017 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Rabbit_IgG__H+L__AF546/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-31572 | AF555 | AB_162543 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Zebrafish | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21245 | AF647 | AB_2535813 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Rabbit_IgG__H+L__AF647/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21206 | AF488 | AB_2535792 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Human | Goat | Fab2 | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | Z25305 | AF555 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-3882-457X](supporting_material/Rabbit_IgG__H+L__AF555/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [AAT Bioquest](https://www.aatbio.com/) | 16686 | iF532 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__iF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-31572 | AF555 | AB_162543 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [AAT Bioquest](https://www.aatbio.com/) | 16833 | iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21206 | AF488 | AB_2535792 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21038 | AF700 | AB_2535709 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Human | Goat | Fab2 | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | Z25308 | AF647 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Mouse | Goat | Fab2 | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | Z25307 | AF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-1431-1398](supporting_material/Rabbit_IgG__H+L__AF594/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | -| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Human | Goat | Fab2 | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | Z25303 | AF532 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Human | Goat | Fab2 | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | Z25302 | AF488 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-31573 | AF647 | AB_2536183 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21039 | AF750 | AB_2535710 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF750/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21245 | AF647 | AB_2535813 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21428 | AF555 | AB_2535849 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [AAT Bioquest](https://www.aatbio.com/) | 16690 | iF555 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__iF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21206 | AF488 | AB_2535792 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Mouse | Goat | Fab2 | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | Z25303 | AF532 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-1431-1398](supporting_material/Rabbit_IgG__H+L__AF532/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11009 | AF532 | AB_2534076 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [AAT Bioquest](https://www.aatbio.com/) | 16712 | iF680 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__iF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [AAT Bioquest](https://www.aatbio.com/) | 16720 | iF750 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__iF750/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11037 | AF594 | AB_2534095 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21206 | AF488 | AB_2535792 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21207 | AF594 | AB_141637 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21074 | AF660 | AB_2535735 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF660/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21207 | AF594 | AB_141637 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-10043 | AF680 | AB_2534018 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [AAT Bioquest](https://www.aatbio.com/) | 16720 | iF750 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__iF750/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11009 | AF532 | AB_2534076 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | [AAT Bioquest](https://www.aatbio.com/) | 16710 | iF647 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__iF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 712-625-150 | AF680 | AB_2340698 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rat IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21096 | AF680 | AB_2535750 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rat IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11007 | AF594 | AB_10561522 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rabbit_IgG__H+L__AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Rat IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21094 | AF633 | AB_2535749 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF633/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 712-585-150 | AF594 | AB_2340688 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-48270 | AF555 (Plus) | AB_2896336 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF555__Plus_/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | SA5-10031 | DL755 | AB_2556611 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__DL755/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 712-605-153 | AF647 | AB_2340694 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 712-605-153 | AF647 | AB_2340694 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rat IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21247 | AF647 | AB_141778 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 712-605-153 | AF647 | AB_2340694 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | SA5-10031 | DL755 | AB_2556611 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__DL755/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-48272 | AF647 (Plus) | AB_2893138 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF647__Plus_/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-48269 | AF488 (Plus) | AB_2893137 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF488__Plus_/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-48270 | AF555 (Plus) | AB_2896336 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF555__Plus_/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rat IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21096 | AF680 | AB_2535750 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 712-605-153 | AF647 | AB_2340694 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 712-625-150 | AF680 | AB_2340698 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 712-585-150 | AF594 | AB_2340688 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 712-545-150 | AF488 | AB_2340683 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rat IgG (H+L) | Mouse | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21247 | AF647 | AB_141778 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-48272 | AF647 (Plus) | AB_2893138 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF647__Plus_/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Jackson ImmunoResearch](https://www.jacksonimmuno.com/) | 712-605-153 | AF647 | AB_2340694 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Rat IgG (H+L) | Human | Goat | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11007 | AF594 | AB_10561522 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/Rat_IgG__H+L__AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q6PBW5](https://www.uniprot.org/uniprotkb/Q6PBW5) | Primary Antibody | Retinal pigment epithelium-specific protein 65kDa | Zebrafish | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 17939-1-AP | Unconjugated | AB_2285290 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Retinal_pigment_epithelium-specific_protein_65kDa_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [Q6P027](https://www.uniprot.org/uniprotkb/Q6P027) | Primary Antibody | Retinaldehyde binding protein 1 | Zebrafish | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 15356-1-AP | Unconjugated | AB_2178530 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Retinaldehyde_binding_protein_1_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [P35359](https://www.uniprot.org/uniprotkb/P35359) | Primary Antibody | Rhodopsin | Zebrafish | Mouse | IgG1 | 1D4 | [Santa Cruz Biotechnology](https://www.scbt.com/) | sc-57432 | Unconjugated | AB_785511 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Rhodopsin_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | @@ -1131,87 +1137,88 @@ Additional information on fluorescent probes tested by the IBEX Imaging Communit | [P51449](https://www.uniprot.org/uniprotkb/P51449) | Primary Antibody | RORgamma t | Human | Mouse | IgG2a | 6F3.1 | [MilliporeSigma](https://www.sigmaaldrich.com/) | MABF81 | Unconjugated | AB_11205416 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/RORgamma_t_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | SARS-NP | Rhesus Macaque | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NB100-56576 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lung | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | [0000-0003-0666-4804](supporting_material/SARS-NP_Unconjugated/0000-0003-0666-4804.md) | NA | 0000-0003-0666-4804 | | [O09126](https://www.uniprot.org/uniprotkb/O09126) | Primary Antibody | SEMA4D | Mouse | Rat | IgG2a | BMA-12 | [BioLegend](https://www.biolegend.com/) | 147609 | AF594 | AB_2687303 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0002-7422-3237](supporting_material/SEMA4D_AF594/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | -| NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21102 | AF680 | AB_2535755 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Sheep_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21448 | AF647 | AB_2535865 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Sheep_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21102 | AF680 | AB_2535755 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Sheep_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21102 | AF680 | AB_2535755 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Sheep_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21102 | AF680 | AB_2535755 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Sheep_IgG__H+L__AF680/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-11015 | AF488 | AB_2534082 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Sheep_IgG__H+L__AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21436 | AF555 | AB_2535857 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Sheep_IgG__H+L__AF555/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | A-21448 | AF647 | AB_2535865 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Sheep_IgG__H+L__AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q920G3](https://www.uniprot.org/uniprotkb/Q920G3) | Primary Antibody | SiglecF | Mouse | Rat | IgG2a | 1RNM44N | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 56-1702-80 | AF700 | AB_2637126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/SiglecF_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q920G3](https://www.uniprot.org/uniprotkb/Q920G3) | Primary Antibody | SiglecF | Mouse | Rat | IgG2a | E50-2440 | [BD Biosciences](https://www.bdbiosciences.com/) | 552126 | PE | AB_394341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/SiglecF_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P97797](https://www.uniprot.org/uniprotkb/P97797) | Primary Antibody | SIRPalpha | Mouse | Rat | IgG1 | P84 | [BioLegend](https://www.biolegend.com/) | 144027 | AF647 | AB_2721300 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/SIRPalpha_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q920G3](https://www.uniprot.org/uniprotkb/Q920G3) | Primary Antibody | SiglecF | Mouse | Rat | IgG2a | E50-2440 | [BD Biosciences](https://www.bdbiosciences.com/) | 552126 | PE | AB_394341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0001-5813-2581](supporting_material/SiglecF_PE/0000-0001-5813-2581.md) | NA | 0000-0001-5813-2581 | +| [Q920G3](https://www.uniprot.org/uniprotkb/Q920G3) | Primary Antibody | SiglecF | Mouse | Rat | IgG2a | 1RNM44N | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 56-1702-80 | AF700 | AB_2637126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/SiglecF_AF700/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P97797](https://www.uniprot.org/uniprotkb/P97797) | Primary Antibody | SIRPalpha | Mouse | Rat | IgG1 | P84 | [BioLegend](https://www.biolegend.com/) | 144024 | AF488 | AB_2650815 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/SIRPalpha_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P97797](https://www.uniprot.org/uniprotkb/P97797) | Primary Antibody | SIRPalpha | Mouse | Rat | IgG1 | P84 | [BioLegend](https://www.biolegend.com/) | 144024 | AF488 | AB_2650815 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/SIRPalpha_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P97797](https://www.uniprot.org/uniprotkb/P97797) | Primary Antibody | SIRPalpha | Mouse | Rat | IgG1 | P84 | [BioLegend](https://www.biolegend.com/) | 144027 | AF647 | AB_2721300 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/SIRPalpha_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P05023](https://www.uniprot.org/uniprotkb/P05023) | Primary Antibody | Sodium Potassium ATPase | Human | Rabbit | IgG | EP1845Y | [Abcam](https://www.abcam.com/) | ab198367 | AF647 | AB_2916056 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-3697-5726](supporting_material/Sodium_Potassium_ATPase_AF647/0000-0003-3697-5726.md) | NA | 0000-0003-3697-5726 | | [P05023](https://www.uniprot.org/uniprotkb/P05023) | Primary Antibody | Sodium Potassium ATPase | Human | Rabbit | IgG | EP1845Y | [Abcam](https://www.abcam.com/) | ab197713 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/Sodium_Potassium_ATPase_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q06945](https://www.uniprot.org/uniprotkb/Q06945) | Primary Antibody | SOX4 | Human | Mouse | IgG2b | SOX4/2540 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-79903AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](supporting_material/SOX4_AF488/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | | [Q06945](https://www.uniprot.org/uniprotkb/Q06945) | Primary Antibody | SOX4 | Human | Mouse | IgG2b | CL5634 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-61420 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](supporting_material/SOX4_Unconjugated/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | +| [Q06945](https://www.uniprot.org/uniprotkb/Q06945) | Primary Antibody | SOX4 | Human | Mouse | IgG2b | SOX4/2540 | [Novus Biologicals](https://www.novusbio.com/) | NBP2-79903AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0002-2950-2683](supporting_material/SOX4_AF488/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | | [Q15506](https://www.uniprot.org/uniprotkb/Q15506) | Primary Antibody | SPA17 | Human | Rabbit | IgG | Polyclonal | [Proteintech](https://www.ptglab.com/) | 13367-1-AP | Unconjugated | AB_2194631 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/SPA17_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P09486](https://www.uniprot.org/uniprotkb/P09486) | Primary Antibody | SPARC | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF941 (Unconjugated) | AF532 | AB_2892754 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/SPARC_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P09486](https://www.uniprot.org/uniprotkb/P09486) | Primary Antibody | SPARC | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF941 (Unconjugated) | AF532 | AB_2892754 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/SPARC_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P09486](https://www.uniprot.org/uniprotkb/P09486) | Primary Antibody | SPARC | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF941 (Unconjugated) | AF532 | AB_2892754 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/SPARC_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P09486](https://www.uniprot.org/uniprotkb/P09486) | Primary Antibody | SPARC | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF941 (Unconjugated) | AF532 | AB_2892754 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/SPARC_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P09486](https://www.uniprot.org/uniprotkb/P09486) | Primary Antibody | SPARC | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF941 | Unconjugated | AB_355728 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/SPARC_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P14719](https://www.uniprot.org/uniprotkb/P14719) | Primary Antibody | ST2 (IL-33R) | Mouse | Rat | IgG2a | U29-93 | [BD Biosciences](https://www.bdbiosciences.com/) | 566311 | PE | AB_2744490 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-1461-0999](supporting_material/ST2__IL-33R__PE/0000-0002-1461-0999.md) | NA | 0000-0002-1461-0999 | | [P18827](https://www.uniprot.org/uniprotkb/P18827) | Primary Antibody | Syndecan-1 | Human | Goat | IgG | Polyclonal | [R&D Systems](https://www.rndsystems.com/) | AF2780 (Unconjugated) | iF594 | AB_2892765 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Syndecan-1_iF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | TCR gamma delta | Human | Mouse | IgG1 | B1 | [BioLegend](https://www.biolegend.com/) | 331225 | PE/Dazzle AF594 | AB_2565533 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/TCR_gamma_delta_PE_Dazzle_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | TCR gamma delta | Mouse | Armenian Hamster | IgG | GL3 | [BioLegend](https://www.biolegend.com/) | 118128 | AF488 | AB_2562771 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/TCR_gamma_delta_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | TCR gamma delta | Mouse | Armenian Hamster | IgG | GL3 | [BioLegend](https://www.biolegend.com/) | 118108 | PE | AB_313832 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/TCR_gamma_delta_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | TCR gamma delta | Human | Mouse | IgG1 | B1 | [BioLegend](https://www.biolegend.com/) | 331210 | PE | AB_1089218 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/TCR_gamma_delta_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Primary Antibody | TCR gamma delta | Mouse | Armenian Hamster | IgG | GL3 | [BioLegend](https://www.biolegend.com/) | 118108 | PE | AB_313832 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/TCR_gamma_delta_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | TCR gamma delta | Human | Mouse | IgG1 | B1 | [BioLegend](https://www.biolegend.com/) | 331210 | PE | AB_1089218 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/TCR_gamma_delta_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | TCR gamma delta | Mouse | Armenian Hamster | IgG | GL3 | [BioLegend](https://www.biolegend.com/) | 118128 | AF488 | AB_2562771 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/TCR_gamma_delta_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | TCR gamma delta | Human | Mouse | IgG1 | B1 | [BioLegend](https://www.biolegend.com/) | 331225 | PE/Dazzle AF594 | AB_2565533 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/TCR_gamma_delta_PE_Dazzle_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | TCR gamma delta | Mouse | Armenian Hamster | IgG | GL3 | [BioLegend](https://www.biolegend.com/) | 118108 | PE | AB_313832 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/TCR_gamma_delta_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Primary Antibody | TCR gamma delta | Mouse | Armenian Hamster | IgG | GL3 | [BioLegend](https://www.biolegend.com/) | 118108 | PE | AB_313832 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/TCR_gamma_delta_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | TCR Valpha24 | Human | Mouse | IgG1 | 6B11 | [BioLegend](https://www.biolegend.com/) | 342919 | PE/Dazzle AF594 | AB_2566311 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/TCR_Valpha24_PE_Dazzle_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | TCR Valpha7.2 | Human | Mouse | IgG1 | 3C10 | [BioLegend](https://www.biolegend.com/) | 351706 | PE | AB_10899577 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/TCR_Valpha7.2_PE/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | TCR Valpha7.2 | Human | Mouse | IgG1 | 3C10 | [BioLegend](https://www.biolegend.com/) | 351729 | PE/Dazzle AF594 | AB_2566338 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/TCR_Valpha7.2_PE_Dazzle_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | TCR Valpha7.2 | Human | Mouse | IgG1 | 3C10 | [BioLegend](https://www.biolegend.com/) | 351726 | AF647 | AB_2566335 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/TCR_Valpha7.2_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9UIF3](https://www.uniprot.org/uniprotkb/Q9UIF3) | Primary Antibody | TEKT2 | Human | Rabbit | IgG | Polyclonal | [Novus Biologicals](https://www.novusbio.com/) | NBP1-86955 | Unconjugated | AB_11017402 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/TEKT2_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q8TDQ0](https://www.uniprot.org/uniprotkb/Q8TDQ0) | Primary Antibody | Tim-3 | Human | Rat | IgG2a | 344823 | [R&D Systems](https://www.rndsystems.com/) | MAB2365 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Tim-3_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q8TDQ0](https://www.uniprot.org/uniprotkb/Q8TDQ0) | Primary Antibody | Tim-3 | Human | Rat | IgG2a | 344823 | [R&D Systems](https://www.rndsystems.com/) | MAB2365 | Unconjugated | AB_2232900 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/Tim-3_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q8TDQ0](https://www.uniprot.org/uniprotkb/Q8TDQ0) | Primary Antibody | Tim-3 | Human | Rat | IgG2a | 344823 | [R&D Systems](https://www.rndsystems.com/) | MAB2365 | Unconjugated | AB_2232900 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Tim-3_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q8TDQ0](https://www.uniprot.org/uniprotkb/Q8TDQ0) | Primary Antibody | Tim-3 | Human | Rabbit | IgG | Polyclonal | [Abcam](https://www.abcam.com/) | ab185703 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | [0000-0003-4379-8967](supporting_material/Tim-3_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q8TDQ0](https://www.uniprot.org/uniprotkb/Q8TDQ0) | Primary Antibody | Tim-3 | Human | Rat | IgG2a | 344823 | [R&D Systems](https://www.rndsystems.com/) | MAB2365 | Unconjugated | AB_2232900 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0000-0003-4379-8967](supporting_material/Tim-3_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q8TDQ0](https://www.uniprot.org/uniprotkb/Q8TDQ0) | Primary Antibody | Tim-3 | Human | Rat | IgG2a | 344823 | [R&D Systems](https://www.rndsystems.com/) | MAB2365 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Tim-3_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q6U7R4](https://www.uniprot.org/uniprotkb/Q6U7R4) | Primary Antibody | Tim-4 | Mouse | Rat | IgG1 | 21H12 | [BD Biosciences](https://www.bdbiosciences.com/) | 742773 | BV421 | AB_2741037 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-1431-1398](supporting_material/Tim-4_BV421/0000-0003-1431-1398.md) | NA | 0000-0003-1431-1398 | | [O15393](https://www.uniprot.org/uniprotkb/O15393) | Primary Antibody | TMPRSS2 | Human | Rabbit | IgG | EPR3861 | [Abcam](https://www.abcam.com/) | ab92323 | Unconjugated | AB_10585592 | Stock | Multiplexed 2D Imaging | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/TMPRSS2_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [O15393](https://www.uniprot.org/uniprotkb/O15393) | Primary Antibody | TMPRSS2 | Human | Rabbit | IgG | EPR3861 | [Abcam](https://www.abcam.com/) | ab92323 (Unconjugated) | AF647 | AB_2892763 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/TMPRSS2_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q15661](https://www.uniprot.org/uniprotkb/Q15661) | Primary Antibody | Tryptase | Human | Mouse | IgG1 | AA1 | [Abcam](https://www.abcam.com/) | ab2378 | Unconjugated | AB_303023 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Tryptase_Unconjugated/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q13509](https://www.uniprot.org/uniprotkb/Q13509) | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | TUJ1 | [BioLegend](https://www.biolegend.com/) | 801203 | AF488 | AB_2564757 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/Tubulin_beta-3_chain_AF488/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | -| [Q9ERD7](https://www.uniprot.org/uniprotkb/Q9ERD7) | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | AA10 | [BioLegend](https://www.biolegend.com/) | 657402 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Tubulin_beta-3_chain_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q4QRB4](https://www.uniprot.org/uniprotkb/Q4QRB4) | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | TUJ1 | [BioLegend](https://www.biolegend.com/) | 801203 | AF488 | AB_2564757 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/Tubulin_beta-3_chain_AF488/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | +| [Q13509](https://www.uniprot.org/uniprotkb/Q13509) | Primary Antibody | Tubulin beta-3 chain | Human | Mouse | IgG2a | AA10 | [BioLegend](https://www.biolegend.com/) | 657412 | BV421 | AB_2632699 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Tubulin_beta-3_chain_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q13509](https://www.uniprot.org/uniprotkb/Q13509) | Primary Antibody | Tubulin beta-3 chain | Human | Mouse | IgG2a | TUJ1 | [BioLegend](https://www.biolegend.com/) | 801202 (Unconjugated) | AF532 | AB_2892767 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-3882-457X](supporting_material/Tubulin_beta-3_chain_AF532/0000-0002-3882-457X.md) | NA | 0000-0002-3882-457X | +| [Q13509](https://www.uniprot.org/uniprotkb/Q13509) | Primary Antibody | Tubulin beta-3 chain | Human | Mouse | IgG2a | TUJ1 | [BioLegend](https://www.biolegend.com/) | 801203 | AF488 | AB_2564757 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/Tubulin_beta-3_chain_AF488/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [Q13509](https://www.uniprot.org/uniprotkb/Q13509) | Primary Antibody | Tubulin beta-3 chain | Human | Mouse | IgG2a | TUJ1 | [BioLegend](https://www.biolegend.com/) | 801203 | AF488 | AB_2564757 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Tubulin_beta-3_chain_AF488/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [Q4QRB4](https://www.uniprot.org/uniprotkb/Q4QRB4) | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | TUJ1 | [BioLegend](https://www.biolegend.com/) | 801203 | AF488 | AB_2564757 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/Tubulin_beta-3_chain_AF488/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | | [Q9ERD7](https://www.uniprot.org/uniprotkb/Q9ERD7) | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | AA10 | [BioLegend](https://www.biolegend.com/) | 657402 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Tubulin_beta-3_chain_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q13509](https://www.uniprot.org/uniprotkb/Q13509) | Primary Antibody | Tubulin beta-3 chain | Human | Mouse | IgG2a | TUJ1 | [BioLegend](https://www.biolegend.com/) | 801203 | AF488 | AB_2564757 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/Tubulin_beta-3_chain_AF488/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | +| [Q13509](https://www.uniprot.org/uniprotkb/Q13509) | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | TUJ1 | [BioLegend](https://www.biolegend.com/) | 801203 | AF488 | AB_2564757 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-7422-3237](supporting_material/Tubulin_beta-3_chain_AF488/0000-0002-7422-3237.md) | NA | 0000-0002-7422-3237 | +| [Q9ERD7](https://www.uniprot.org/uniprotkb/Q9ERD7) | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | AA10 | [BioLegend](https://www.biolegend.com/) | 657402 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Tubulin_beta-3_chain_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9ERD7](https://www.uniprot.org/uniprotkb/Q9ERD7) | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | AA10 | [BioLegend](https://www.biolegend.com/) | 657412 | BV421 | AB_2632699 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](supporting_material/Tubulin_beta-3_chain_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [Q13509](https://www.uniprot.org/uniprotkb/Q13509) | Primary Antibody | Tubulin beta-3 chain | Human | Mouse | IgG2a | AA10 | [BioLegend](https://www.biolegend.com/) | 657412 | BV421 | AB_2632699 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](supporting_material/Tubulin_beta-3_chain_BV421/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P07911](https://www.uniprot.org/uniprotkb/P07911) | Primary Antibody | Uromodulin | Human | Mouse | IgG2b | 10.32 | [Novus Biologicals](https://www.novusbio.com/) | NBP1-50431 | FITC | AB_10012622 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Uromodulin_FITC/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | UT014 | Human | Rabbit | IgG | Polyclonal | [Cell IDx](https://cellidx.com/) | HI06B-005 | CL550 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/UT014_CL550/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | UT014 | Human | Rabbit | IgG | Polyclonal | [Cell IDx](https://cellidx.com/) | HI06B-005 | CL550 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/UT014_CL550/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| NA | Secondary Antibody | UT015 | Human | Rabbit | IgG | Polyclonal | [Cell IDx](https://cellidx.com/) | HI06B-005 | CL490 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/UT015_CL490/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | UT015 | Human | Rabbit | IgG | Polyclonal | [Cell IDx](https://cellidx.com/) | HI06B-005 | CL490 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/UT015_CL490/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| NA | Secondary Antibody | UT015 | Human | Rabbit | IgG | Polyclonal | [Cell IDx](https://cellidx.com/) | HI06B-005 | CL490 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/UT015_CL490/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | UT016 | Human | Rabbit | IgG | Polyclonal | [Cell IDx](https://cellidx.com/) | HI06B-005 | CL650 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/UT016_CL650/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Secondary Antibody | UT019 | Human | Rabbit | IgG | Polyclonal | [Cell IDx](https://cellidx.com/) | HI06B-005 | CL594 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | [0000-0003-4379-8967](supporting_material/UT019_CL594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [Q9W6A9](https://www.uniprot.org/uniprotkb/Q9W6A9) | Primary Antibody | UV opsin | Zebrafish | Rabbit | IgG | Polyclonal | [Kerafast](https://www.kerafast.com/) | EJH013 | Unconjugated | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/UV_opsin_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [P33151](https://www.uniprot.org/uniprotkb/P33151) | Primary Antibody | VE-Cadherin | Human | Mouse | IgG2a | BV9 | [BioLegend](https://www.biolegend.com/) | 348512 | AF594 | AB_2563354 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0002-2950-2683](supporting_material/VE-Cadherin_AF594/0000-0002-2950-2683.md) | NA | 0000-0002-2950-2683 | -| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677802 (Unconjugated) | AF532 | AB_2892753 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677802 (Unconjugated) | AF532 | AB_2892753 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677804 | AF594 | AB_2566179 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677804 | AF594 | AB_2566179 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/Vimentin_AF647/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | -| [P20152](https://www.uniprot.org/uniprotkb/P20152) | Primary Antibody | Vimentin | Mouse | Rat | IgG2a | W16220A | [BioLegend](https://www.biolegend.com/) | 699305 | AF488 | AB_2888889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/Vimentin_AF488/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | -| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P20152](https://www.uniprot.org/uniprotkb/P20152) | Primary Antibody | Vimentin | Mouse | Rat | IgG2a | W16220A | [BioLegend](https://www.biolegend.com/) | 699308 | AF647 | AB_2888890 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0001-5813-2581](supporting_material/Vimentin_AF647/0000-0001-5813-2581.md) | NA | 0000-0001-5813-2581 | -| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677804 | AF594 | AB_2566179 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-3697-5726](supporting_material/Vimentin_AF594/0000-0003-3697-5726.md) | NA | 0000-0003-3697-5726 | -| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677807 | AF647 | AB_2616801 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677807 | AF647 | AB_2616801 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677807 | AF647 | AB_2616801 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677807 | AF647 | AB_2616801 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677804 | AF594 | AB_2566179 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-3697-5726](supporting_material/Vimentin_AF594/0000-0003-3697-5726.md) | NA | 0000-0003-3697-5726 | | [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677802 (Unconjugated) | BL759/780 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_BL759_780/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677802 (Unconjugated) | BL759/780 | NA | Custom | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_BL759_780/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | -| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677807 | AF647 | AB_2616801 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677804 | AF594 | AB_2566179 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677802 (Unconjugated) | AF532 | AB_2892753 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677804 | AF594 | AB_2566179 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF594/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF647/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | +| [P20152](https://www.uniprot.org/uniprotkb/P20152) | Primary Antibody | Vimentin | Mouse | Rat | IgG2a | W16220A | [BioLegend](https://www.biolegend.com/) | 699308 | AF647 | AB_2888890 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0001-5813-2581](supporting_material/Vimentin_AF647/0000-0001-5813-2581.md) | NA | 0000-0001-5813-2581 | +| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0002-2692-1225](supporting_material/Vimentin_AF647/0000-0002-2692-1225.md) | NA | 0000-0002-2692-1225 | +| [P20152](https://www.uniprot.org/uniprotkb/P20152) | Primary Antibody | Vimentin | Mouse | Rat | IgG2a | W16220A | [BioLegend](https://www.biolegend.com/) | 699305 | AF488 | AB_2888889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-2088-8310](supporting_material/Vimentin_AF488/0000-0003-2088-8310.md) | NA | 0000-0003-2088-8310 | +| [P08670](https://www.uniprot.org/uniprotkb/P08670) | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | [BioLegend](https://www.biolegend.com/) | 677802 (Unconjugated) | AF532 | AB_2892753 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](supporting_material/Vimentin_AF532/0000-0003-4379-8967.md) | NA | 0000-0003-4379-8967 | | NA | Primary Antibody | ZO-1 | Zebrafish | Mouse | IgG1 | ZO1-1A12 | [Thermo Fisher Scientific](https://www.thermofisher.com/us/en/home/life-science/antibodies.html) | 33-9100 | Unconjugated | AB_2533147 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/ZO-1_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | NA | Primary Antibody | Zpr-1 | Turquoise Killifish | Mouse | IgG1 | Monoclonal | [ZIRC](https://zebrafish.org) | ZDB-ATB-081002-43 | Unconjugated | AB_10013803 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Zpr-1_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [Q6DH07](https://www.uniprot.org/uniprotkb/Q6DH07) | Primary Antibody | Zpr-1 | Zebrafish | Mouse | IgG1 | Monoclonal | [ZIRC](https://zebrafish.org) | ZDB-ATB-081002-43 | Unconjugated | AB_10013803 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Zpr-1_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | -| [P35359](https://www.uniprot.org/uniprotkb/P35359) | Primary Antibody | Zpr-3 | Zebrafish | Mouse | IgG1 | Monoclonal | [ZIRC](https://zebrafish.org) | ZDB-ATB-081002-45 | Unconjugated | AB_10013805 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Zpr-3_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | NA | Primary Antibody | Zpr-3 | African Clawed Frog (Xenopus laevis) | Mouse | IgG1 | Monoclonal | [ZIRC](https://zebrafish.org) | ZDB-ATB-081002-45 | Unconjugated | AB_10013805 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Zpr-3_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | +| [P35359](https://www.uniprot.org/uniprotkb/P35359) | Primary Antibody | Zpr-3 | Zebrafish | Mouse | IgG1 | Monoclonal | [ZIRC](https://zebrafish.org) | ZDB-ATB-081002-45 | Unconjugated | AB_10013805 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Zpr-3_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | [Q58EE9](https://www.uniprot.org/uniprotkb/Q58EE9) | Primary Antibody | Zrf-1 | Zebrafish | Mouse | IgG1 | Monoclonal | [ZIRC](https://zebrafish.org) | ZDB-ATB-081002-46 | Unconjugated | AB_10013806 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Zrf-1_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | | NA | Primary Antibody | Zrf-1 | Turquoise Killifish | Mouse | IgG1 | Monoclonal | [ZIRC](https://zebrafish.org) | ZDB-ATB-081002-46 | Unconjugated | AB_10013806 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | [0009-0000-2047-4228](supporting_material/Zrf-1_Unconjugated/0009-0000-2047-4228.md) | NA | 0009-0000-2047-4228 | {: #reagent_resources_table_id}