From 6ca992d42d17e108258d813db5efa8a4ccf08796 Mon Sep 17 00:00:00 2001 From: Rosa Kortekaas <177236990+Rosa-234@users.noreply.github.com> Date: Thu, 5 Sep 2024 22:28:18 -0400 Subject: [PATCH 1/3] Added pSMAD3 for Human Lung --- data/reagent_resources.csv | 3 ++- .../0000-0002-8728-1735.md | 22 ++++++++++++++++++ .../pSMAD3_AF647_Human_Lung.jpg | Bin 0 -> 219335 bytes 3 files changed, 24 insertions(+), 1 deletion(-) create mode 100644 docs/supporting_material/Phospho-SMAD3_Unconjugated/0000-0002-8728-1735.md create mode 100644 docs/supporting_material/Phospho-SMAD3_Unconjugated/pSMAD3_AF647_Human_Lung.jpg diff --git a/data/reagent_resources.csv b/data/reagent_resources.csv index 9b094f9..fc37691 100644 --- a/data/reagent_resources.csv +++ b/data/reagent_resources.csv @@ -1205,4 +1205,5 @@ P06800,Primary Antibody,CD45,Mouse,Rat,IgG2b,30-F11,BioLegend,103144,AF594,AB_25 Q920G3,Primary Antibody,SiglecF,Mouse,Rat,IgG2a,E50-2440,BD Biosciences,552126,PE,AB_394341,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lung,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0001-5813-2581,NA,0000-0001-5813-2581 P62737,Primary Antibody,Alpha smooth muscle actin,Mouse,Mouse,IgG2a,1A4,Thermo Fisher Scientific,50-9760-82,eF660,AB_2574362,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lung,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0001-5813-2581,NA,0000-0001-5813-2581 P05089,Primary Antibody,Arginase-1,Mouse,Rabbit,IgG,D4E3M,Cell Signaling Technology,93668,Unconjugated,AB_2800207,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lung,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0001-5813-2581,NA,0000-0001-5813-2581 -Q61830,Primary Antibody,CD206,Mouse,Rat,IgG2a,C068C2,BioLegend,141709,AF488,AB_10933252,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lung,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0001-5813-2581,NA,0000-0001-5813-2581 \ No newline at end of file +Q61830,Primary Antibody,CD206,Mouse,Rat,IgG2a,C068C2,BioLegend,141709,AF488,AB_10933252,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lung,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0001-5813-2581,NA,0000-0001-5813-2581 +NA,Primary Antibody,Phospho-SMAD3,Human,Rabbit,IgG,EP823Y,Abcam,ab52903,Unconjugated,AB_882596,Stock,IBEX2D Manual,FFPE,Lung,IPF,0.3% Triton-X-100,10 mM citrate buffer (pH 6.0) for 30 minutes at 95C,NA,Yes,0000-0002-8728-1735,NA,0000-0002-8728-1735 \ No newline at end of file diff --git a/docs/supporting_material/Phospho-SMAD3_Unconjugated/0000-0002-8728-1735.md b/docs/supporting_material/Phospho-SMAD3_Unconjugated/0000-0002-8728-1735.md new file mode 100644 index 0000000..e93d043 --- /dev/null +++ b/docs/supporting_material/Phospho-SMAD3_Unconjugated/0000-0002-8728-1735.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|------------:|:--------------------------|-----------------:|:-------------|:----------|:---------------|:-----------------------|:----------------------|:----------------|:--------------------|:------------------|:-------------------------------------------------------------------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| +| NA | Primary Antibody | Phospho-SMAD3 | Human | Rabbit | IgG | EP823Y | Abcam | ab52903 | Unconjugated | AB_882596 | Stock | IBEX2D Manual | FFPE | Lung | IPF | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | [0000-0002-8728-1735](https://orcid.org/0000-0002-8728-1735) | NA | [0000-0002-8728-1735](https://orcid.org/0000-0002-8728-1735) | [1](#notes) | + +# Publications + + + +# Additional Notes + + +1. This antibody labels the anticipated subcellular compartment (nucleus). This antibody was detected using a secondary antibody (Abcam, ab150079). We used a secondary only control to test for non-specific binding of the secondary antibody and observerd no signal as expected (not shown). The following antibody was tested for Phospho-SMAD3 and is not recommended: Cell Signaling Technology, 9520. + +| Human IPF lung FFPE: Phospho-SMAD3 (magenta) and DAPI (blue) | +|:-------:| +| ![](pSMAD3_AF647_Human_Lung.jpg) | diff --git a/docs/supporting_material/Phospho-SMAD3_Unconjugated/pSMAD3_AF647_Human_Lung.jpg b/docs/supporting_material/Phospho-SMAD3_Unconjugated/pSMAD3_AF647_Human_Lung.jpg new file mode 100644 index 0000000000000000000000000000000000000000..d687fd322338391c8bb815808a9e0039e4d60ed5 GIT binary patch literal 219335 zcmeFZ2UL?w^EezJ^denAz|eb12qiS>y$7T#kOF~F6PlvZk=~Ie(xij*VnuotX-cus z6$BM5SM)011D1R5`@X+&zVCeJ{LlHjoM+3-?Ck99?mWp%mV>#2W#(_XAvi1uWNHc$ z2Z2CTAW9Nu5E)P-0scTFTp;qJItb)Oa^hFrlSK3f4Jkka1pzteB=7`~z>eyyKs`MU z2Lk`-TLqLeAeukJK_C&vpQR?+*9`-)@bD%25{l%N9?*~5vpair6Iw}w54t79vN3%GqZ3-eicnxAWYJt$s6%`dV6&*D-9TP1LEfd=@I=W+Q94stsEG!&MbVun&@xAe{ISD;2EjtV*g`n?O&|AxUqH;9P}R17L6BjE>;GLeumksLe)CP{P9M{){~lAPoy0RkT; 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literal 0 HcmV?d00001 From a0aaa46661425fce5ede6f7a57555fb65a929fcc Mon Sep 17 00:00:00 2001 From: Rosa Kortekaas <177236990+Rosa-234@users.noreply.github.com> Date: Tue, 10 Sep 2024 09:13:47 -0400 Subject: [PATCH 2/3] Expand abbreviation for IPF --- data/reagent_resources.csv | 2 +- .../Phospho-SMAD3_Unconjugated/0000-0002-8728-1735.md | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/data/reagent_resources.csv b/data/reagent_resources.csv index fc37691..3d791ed 100644 --- a/data/reagent_resources.csv +++ b/data/reagent_resources.csv @@ -1206,4 +1206,4 @@ Q920G3,Primary Antibody,SiglecF,Mouse,Rat,IgG2a,E50-2440,BD Biosciences,552126,P P62737,Primary Antibody,Alpha smooth muscle actin,Mouse,Mouse,IgG2a,1A4,Thermo Fisher Scientific,50-9760-82,eF660,AB_2574362,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lung,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0001-5813-2581,NA,0000-0001-5813-2581 P05089,Primary Antibody,Arginase-1,Mouse,Rabbit,IgG,D4E3M,Cell Signaling Technology,93668,Unconjugated,AB_2800207,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lung,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0001-5813-2581,NA,0000-0001-5813-2581 Q61830,Primary Antibody,CD206,Mouse,Rat,IgG2a,C068C2,BioLegend,141709,AF488,AB_10933252,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lung,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0001-5813-2581,NA,0000-0001-5813-2581 -NA,Primary Antibody,Phospho-SMAD3,Human,Rabbit,IgG,EP823Y,Abcam,ab52903,Unconjugated,AB_882596,Stock,IBEX2D Manual,FFPE,Lung,IPF,0.3% Triton-X-100,10 mM citrate buffer (pH 6.0) for 30 minutes at 95C,NA,Yes,0000-0002-8728-1735,NA,0000-0002-8728-1735 \ No newline at end of file +NA,Primary Antibody,Phospho-SMAD3,Human,Rabbit,IgG,EP823Y,Abcam,ab52903,Unconjugated,AB_882596,Stock,IBEX2D Manual,FFPE,Lung,Idiopathic Pulmonary Fibrosis,0.3% Triton-X-100,10 mM citrate buffer (pH 6.0) for 30 minutes at 95C,NA,Yes,0000-0002-8728-1735,NA,0000-0002-8728-1735 \ No newline at end of file diff --git a/docs/supporting_material/Phospho-SMAD3_Unconjugated/0000-0002-8728-1735.md b/docs/supporting_material/Phospho-SMAD3_Unconjugated/0000-0002-8728-1735.md index e93d043..722b7da 100644 --- a/docs/supporting_material/Phospho-SMAD3_Unconjugated/0000-0002-8728-1735.md +++ b/docs/supporting_material/Phospho-SMAD3_Unconjugated/0000-0002-8728-1735.md @@ -17,6 +17,6 @@ layout: default 1. This antibody labels the anticipated subcellular compartment (nucleus). This antibody was detected using a secondary antibody (Abcam, ab150079). We used a secondary only control to test for non-specific binding of the secondary antibody and observerd no signal as expected (not shown). The following antibody was tested for Phospho-SMAD3 and is not recommended: Cell Signaling Technology, 9520. -| Human IPF lung FFPE: Phospho-SMAD3 (magenta) and DAPI (blue) | +| Human lung (Idiopathic Pulmonary Fibrosis) FFPE: Phospho-SMAD3 (magenta) and DAPI (blue) | |:-------:| | ![](pSMAD3_AF647_Human_Lung.jpg) | From 075d5c1e6697144243aaeb465f1e8dacb100cf0a Mon Sep 17 00:00:00 2001 From: Ziv Yaniv Date: Wed, 11 Sep 2024 21:42:11 -0400 Subject: [PATCH 3/3] Harmonizing contribution. Adding image information to image_resources.csv, harmonizing content of reagent_resources.csv and supporting material file and adding contributor information to the .zenod.json (credit where credit is due). --- .github/data_validation_configs/reagent_resources.json | 6 ++++-- .zenodo.json | 5 +++++ data/image_resources.csv | 3 ++- .../Phospho-SMAD3_Unconjugated/0000-0002-8728-1735.md | 4 ++-- 4 files changed, 13 insertions(+), 5 deletions(-) diff --git a/.github/data_validation_configs/reagent_resources.json b/.github/data_validation_configs/reagent_resources.json index 7eda03d..99c186f 100644 --- a/.github/data_validation_configs/reagent_resources.json +++ b/.github/data_validation_configs/reagent_resources.json @@ -220,7 +220,8 @@ "NA", "Tumor", "GFP transgenic line", - "Melanoma" + "Melanoma", + "Idiopathic Pulmonary Fibrosis" ], "Detergent":[ "0.1% Saponin", @@ -239,7 +240,8 @@ "pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000)", "pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker", "pH 6 (Sodium Citrate buffer) for 45 minutes", - "pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond" + "pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond", + "10 mM citrate buffer (pH 6.0) for 30 minutes at 95C" ], "Dye Inactivation Conditions":[ "0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol", diff --git a/.zenodo.json b/.zenodo.json index 29c0209..d28240f 100644 --- a/.zenodo.json +++ b/.zenodo.json @@ -132,6 +132,11 @@ "name": "Koenig, Joshua F. E.", "orcid": "0000-0002-8909-5039" }, + { + "affiliation": "McMaster University", + "name": "Kortekaas, Rosa K.", + "orcid": "0000-0002-8728-1735" + }, { "affiliation": "University College London", "name": "Kothurkar, Aanandita", diff --git a/data/image_resources.csv b/data/image_resources.csv index 503f9d7..696d194 100644 --- a/data/image_resources.csv +++ b/data/image_resources.csv @@ -211,4 +211,5 @@ CD4_AF488/Human_tonsil_FFPE_CD4_AF488_FAB8165G.jpg,"Human tonsil FFPE: CD4 (gree CXCR3_AF700/Human_Tonsil_FFPE_CXCR3_FAB160N.jpg,"Human tonsil FFPE: CXCR3 (cyan, catalog number FAB160N)",282723d37e8b3bfc2fe3a57fa805f7bc,['CXCR3_AF700/0000-0002-6604-2065.md'] CD20_eF615/Human_Tonsil_FFPE_CD20_eF615_42-0202-82.jpg,"Human tonsil FFPE: CD20 (red, catalog number 42-0202-82)",77e6ac7e3a9009daa030e9f17c118f0c,['CD20_eF615/0000-0002-6604-2065.md'] E-cadherin_AF647/Ecadherin_Yellow_DAPI_Cyan_EpCAM_Blue.jpg,"Human Nasal Polyp: E-Cadherin (yellow, catalog number 147307), EpCAM (blue, catalog number 324228) and DAPI (cyan)",9b79c06f612c26ba7af3cb03c4e21298,['E-cadherin_AF647/0009-0004-8162-409X.md'] -E-cadherin_AF647/Ecadherin_Yellow_DAPI_Cyan.jpg,"Human Nasal Polyp: E-Cadherin (yellow, catalog number 147307), and DAPI (cyan)",4dc47ddf0a00a3ad872b91ba448f914a,['E-cadherin_AF647/0009-0004-8162-409X.md'] \ No newline at end of file +E-cadherin_AF647/Ecadherin_Yellow_DAPI_Cyan.jpg,"Human Nasal Polyp: E-Cadherin (yellow, catalog number 147307), and DAPI (cyan)",4dc47ddf0a00a3ad872b91ba448f914a,['E-cadherin_AF647/0009-0004-8162-409X.md'] +Phospho-SMAD3_Unconjugated/pSMAD3_AF647_Human_Lung.jpg,"Human lung (Idiopathic Pulmonary Fibrosis) FFPE: Phospho-SMAD3 (magenta) and DAPI (blue)",944bcbc69d59d8973393e9c7c04239d2,['Phospho-SMAD3_Unconjugated/0000-0002-8728-1735.md'] \ No newline at end of file diff --git a/docs/supporting_material/Phospho-SMAD3_Unconjugated/0000-0002-8728-1735.md b/docs/supporting_material/Phospho-SMAD3_Unconjugated/0000-0002-8728-1735.md index 722b7da..1c52eac 100644 --- a/docs/supporting_material/Phospho-SMAD3_Unconjugated/0000-0002-8728-1735.md +++ b/docs/supporting_material/Phospho-SMAD3_Unconjugated/0000-0002-8728-1735.md @@ -6,7 +6,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|------------:|:--------------------------|-----------------:|:-------------|:----------|:---------------|:-----------------------|:----------------------|:----------------|:--------------------|:------------------|:-------------------------------------------------------------------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| -| NA | Primary Antibody | Phospho-SMAD3 | Human | Rabbit | IgG | EP823Y | Abcam | ab52903 | Unconjugated | AB_882596 | Stock | IBEX2D Manual | FFPE | Lung | IPF | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | [0000-0002-8728-1735](https://orcid.org/0000-0002-8728-1735) | NA | [0000-0002-8728-1735](https://orcid.org/0000-0002-8728-1735) | [1](#notes) | +| NA | Primary Antibody | Phospho-SMAD3 | Human | Rabbit | IgG | EP823Y | Abcam | ab52903 | Unconjugated | AB_882596 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | [0000-0002-8728-1735](https://orcid.org/0000-0002-8728-1735) | NA | [0000-0002-8728-1735](https://orcid.org/0000-0002-8728-1735) | [1](#notes) | # Publications @@ -15,7 +15,7 @@ layout: default # Additional Notes -1. This antibody labels the anticipated subcellular compartment (nucleus). This antibody was detected using a secondary antibody (Abcam, ab150079). We used a secondary only control to test for non-specific binding of the secondary antibody and observerd no signal as expected (not shown). The following antibody was tested for Phospho-SMAD3 and is not recommended: Cell Signaling Technology, 9520. +1. This antibody labels the anticipated subcellular compartment (nucleus). This antibody was detected using a secondary antibody (Abcam, ab150079). We used a secondary only control to test for non-specific binding of the secondary antibody and observed no signal as expected (not shown). The following antibody was tested for Phospho-SMAD3 and is not recommended: Cell Signaling Technology, 9520. | Human lung (Idiopathic Pulmonary Fibrosis) FFPE: Phospho-SMAD3 (magenta) and DAPI (blue) | |:-------:|