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New tags: GCN4 & GCN4pep #431

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lukasz99 opened this issue Jul 6, 2020 · 1 comment
Open

New tags: GCN4 & GCN4pep #431

lukasz99 opened this issue Jul 6, 2020 · 1 comment

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@lukasz99
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lukasz99 commented Jul 6, 2020

GCN4 is antibody recognizing GCN4pep peptide. The paper

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252608/

uses GCN4pep to tag proteins and GCN4-GFP fusion to detect it.

GCN4 seems to be also used on its own as a tag: See PMID: 32150576

@pporrasebi
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It is not clear to me whether the tag used in PMID:32150576 is the full length GCN4 or the peptide described in the paper you mention. Also, from reading such paper I interpret there could be three potential tags:

  • GCN4 as a tag, possibly (but I think not likely) full length.
  • GCN4 peptide, potentially even single repeat, but more likely multiple repeats (low copy number).
  • Sun Tag: 24 repeats of peptide GCN4 sequence, used to amplify GFP signal in combination with anti-GCN4, GFP-tagged antibodies

Or if these distinctions are deemed not important for the particular use case you have, I can create a generic GNC4 tag and then add specifics if needed. I'd like to avoid creating tags that are used in just one publication, hence the question.

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