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ROIs defined on CZI MIPs are rotated #79

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DrLachie opened this issue Aug 30, 2024 · 0 comments
Open

ROIs defined on CZI MIPs are rotated #79

DrLachie opened this issue Aug 30, 2024 · 0 comments

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@DrLachie
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The MIP image generated via ZEN has been rotated 90 degrees right. If users are using this image to define rois with the roi manager in fiji/imagej then the rois are incorrect when imported into lls-pipeline. We have two workarounds:

  • Before defining ROIs, rotate the image 90 degrees left and draw ROIs on the rotated image
    OR
  • Transform ROIs before importing into lls-pipeline. Attached FIJI macro does this (rename from txt to ijm - requires BIOP and IJPB plugins.
    fix_rois.txt

In future Zen may change rotation - it's changed before. Could add version check and apply fix or raise error to user.

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