Skip to content

Commit

Permalink
Deploying to gh-pages from @ 8da3162 🚀
Browse files Browse the repository at this point in the history
  • Loading branch information
3mmaRand committed Oct 15, 2023
1 parent c07b8c0 commit ddbcd8b
Show file tree
Hide file tree
Showing 3 changed files with 66 additions and 52 deletions.
58 changes: 29 additions & 29 deletions core/week-2/workshop.html
Original file line number Diff line number Diff line change
Expand Up @@ -425,49 +425,49 @@ <h1 class="title">Workshop</h1>
<div class="sourceCode cell-code" id="cb9"><pre class="sourceCode bash code-with-copy"><code class="sourceCode bash"><span id="cb9-1"><a href="#cb9-1" aria-hidden="true" tabindex="-1"></a><span class="fu">ls</span> <span class="at">-l</span></span></code><button title="Copy to Clipboard" class="code-copy-button"><i class="bi"></i></button></pre></div>
<div class="cell-output cell-output-stdout">
<pre><code>total 136
drwxr-xr-x 2 runner docker 4096 Oct 15 09:00 data
drwxr-xr-x 2 runner docker 4096 Oct 15 09:00 images
-rw-r--r-- 1 runner docker 1597 Oct 15 09:00 overview.qmd
-rw-r--r-- 1 runner docker 184 Oct 15 09:00 study_after_workshop.qmd
-rw-r--r-- 1 runner docker 69877 Oct 15 09:04 study_before_workshop.html
-rw-r--r-- 1 runner docker 4807 Oct 15 09:00 study_before_workshop.ipynb
-rw-r--r-- 1 runner docker 13029 Oct 15 09:00 study_before_workshop.qmd
-rw-r--r-- 1 runner docker 8550 Oct 15 09:00 workshop.qmd
-rw-r--r-- 1 runner docker 8564 Oct 15 09:05 workshop.rmarkdown</code></pre>
drwxr-xr-x 2 runner docker 4096 Oct 15 09:26 data
drwxr-xr-x 2 runner docker 4096 Oct 15 09:26 images
-rw-r--r-- 1 runner docker 1597 Oct 15 09:26 overview.qmd
-rw-r--r-- 1 runner docker 184 Oct 15 09:26 study_after_workshop.qmd
-rw-r--r-- 1 runner docker 69877 Oct 15 09:29 study_before_workshop.html
-rw-r--r-- 1 runner docker 4807 Oct 15 09:26 study_before_workshop.ipynb
-rw-r--r-- 1 runner docker 13029 Oct 15 09:26 study_before_workshop.qmd
-rw-r--r-- 1 runner docker 8550 Oct 15 09:26 workshop.qmd
-rw-r--r-- 1 runner docker 8564 Oct 15 09:29 workshop.rmarkdown</code></pre>
</div>
</div>
<p>You can use more than one option at once. The <code>-h</code> option stands for “human readable” and makes the file sizes easier to understand for humans:</p>
<div class="cell">
<div class="sourceCode cell-code" id="cb11"><pre class="sourceCode bash code-with-copy"><code class="sourceCode bash"><span id="cb11-1"><a href="#cb11-1" aria-hidden="true" tabindex="-1"></a><span class="fu">ls</span> <span class="at">-hl</span></span></code><button title="Copy to Clipboard" class="code-copy-button"><i class="bi"></i></button></pre></div>
<div class="cell-output cell-output-stdout">
<pre><code>total 136K
drwxr-xr-x 2 runner docker 4.0K Oct 15 09:00 data
drwxr-xr-x 2 runner docker 4.0K Oct 15 09:00 images
-rw-r--r-- 1 runner docker 1.6K Oct 15 09:00 overview.qmd
-rw-r--r-- 1 runner docker 184 Oct 15 09:00 study_after_workshop.qmd
-rw-r--r-- 1 runner docker 69K Oct 15 09:04 study_before_workshop.html
-rw-r--r-- 1 runner docker 4.7K Oct 15 09:00 study_before_workshop.ipynb
-rw-r--r-- 1 runner docker 13K Oct 15 09:00 study_before_workshop.qmd
-rw-r--r-- 1 runner docker 8.4K Oct 15 09:00 workshop.qmd
-rw-r--r-- 1 runner docker 8.4K Oct 15 09:05 workshop.rmarkdown</code></pre>
drwxr-xr-x 2 runner docker 4.0K Oct 15 09:26 data
drwxr-xr-x 2 runner docker 4.0K Oct 15 09:26 images
-rw-r--r-- 1 runner docker 1.6K Oct 15 09:26 overview.qmd
-rw-r--r-- 1 runner docker 184 Oct 15 09:26 study_after_workshop.qmd
-rw-r--r-- 1 runner docker 69K Oct 15 09:29 study_before_workshop.html
-rw-r--r-- 1 runner docker 4.7K Oct 15 09:26 study_before_workshop.ipynb
-rw-r--r-- 1 runner docker 13K Oct 15 09:26 study_before_workshop.qmd
-rw-r--r-- 1 runner docker 8.4K Oct 15 09:26 workshop.qmd
-rw-r--r-- 1 runner docker 8.4K Oct 15 09:29 workshop.rmarkdown</code></pre>
</div>
</div>
<p>The <code>-a</code> option stands for “all” and shows us all the files, including hidden files.</p>
<div class="cell">
<div class="sourceCode cell-code" id="cb13"><pre class="sourceCode bash code-with-copy"><code class="sourceCode bash"><span id="cb13-1"><a href="#cb13-1" aria-hidden="true" tabindex="-1"></a><span class="fu">ls</span> <span class="at">-alh</span></span></code><button title="Copy to Clipboard" class="code-copy-button"><i class="bi"></i></button></pre></div>
<div class="cell-output cell-output-stdout">
<pre><code>total 144K
drwxr-xr-x 4 runner docker 4.0K Oct 15 09:05 .
drwxr-xr-x 5 runner docker 4.0K Oct 15 09:04 ..
drwxr-xr-x 2 runner docker 4.0K Oct 15 09:00 data
drwxr-xr-x 2 runner docker 4.0K Oct 15 09:00 images
-rw-r--r-- 1 runner docker 1.6K Oct 15 09:00 overview.qmd
-rw-r--r-- 1 runner docker 184 Oct 15 09:00 study_after_workshop.qmd
-rw-r--r-- 1 runner docker 69K Oct 15 09:04 study_before_workshop.html
-rw-r--r-- 1 runner docker 4.7K Oct 15 09:00 study_before_workshop.ipynb
-rw-r--r-- 1 runner docker 13K Oct 15 09:00 study_before_workshop.qmd
-rw-r--r-- 1 runner docker 8.4K Oct 15 09:00 workshop.qmd
-rw-r--r-- 1 runner docker 8.4K Oct 15 09:05 workshop.rmarkdown</code></pre>
drwxr-xr-x 4 runner docker 4.0K Oct 15 09:29 .
drwxr-xr-x 5 runner docker 4.0K Oct 15 09:29 ..
drwxr-xr-x 2 runner docker 4.0K Oct 15 09:26 data
drwxr-xr-x 2 runner docker 4.0K Oct 15 09:26 images
-rw-r--r-- 1 runner docker 1.6K Oct 15 09:26 overview.qmd
-rw-r--r-- 1 runner docker 184 Oct 15 09:26 study_after_workshop.qmd
-rw-r--r-- 1 runner docker 69K Oct 15 09:29 study_before_workshop.html
-rw-r--r-- 1 runner docker 4.7K Oct 15 09:26 study_before_workshop.ipynb
-rw-r--r-- 1 runner docker 13K Oct 15 09:26 study_before_workshop.qmd
-rw-r--r-- 1 runner docker 8.4K Oct 15 09:26 workshop.qmd
-rw-r--r-- 1 runner docker 8.4K Oct 15 09:29 workshop.rmarkdown</code></pre>
</div>
</div>
<p>You can move about with the <code>cd</code> command, which stands for “change directory”. You can use it to move into a directory by specifying the path to the directory:</p>
Expand Down
52 changes: 33 additions & 19 deletions omics/omics.html
Original file line number Diff line number Diff line change
Expand Up @@ -21,7 +21,26 @@
margin: 0 0.8em 0.2em -1em; /* quarto-specific, see https://github.com/quarto-dev/quarto-cli/issues/4556 */
vertical-align: middle;
}
</style>
/* CSS for citations */
div.csl-bib-body { }
div.csl-entry {
clear: both;
}
.hanging-indent div.csl-entry {
margin-left:2em;
text-indent:-2em;
}
div.csl-left-margin {
min-width:2em;
float:left;
}
div.csl-right-inline {
margin-left:2em;
padding-left:1em;
}
div.csl-indent {
margin-left: 2em;
}</style>


<script src="../site_libs/quarto-nav/quarto-nav.js"></script>
Expand Down Expand Up @@ -287,49 +306,44 @@ <h2 class="anchored" data-anchor-id="omics-1-hello-data">Omics 1: 👋 Hello dat
</section>
<section id="omics-2-statistical-analysis" class="level2">
<h2 class="anchored" data-anchor-id="omics-2-statistical-analysis">Omics 2: Statistical Analysis</h2>
<p>before</p>
<ul>
<li>which genes matter: expression, DE</li>
<li>goal of DE</li>
<li>how does DE work</li>
<li>multiple testing: FDR</li>
<li>say what comparisons we will do</li>
</ul>
<p>workshop - open project import the data - revise! - prep for DE - lists of genes - writing to files</p>
<p>after</p>
<ul>
<li>document what you have done</li>
<li>repeat on another comparison</li>
</ul>
<p>This week we cover differential expression analysis on raw counts or log normalised values. The independent study will allow you to check you have what you should have following the <a href="week-3/workshop.html">Omics 1: Hello Data workshop</a> and <a href="week-3/study_after_workshop.html">Consolidation study</a>. It will also summarise the concepts and methods we will use in the workshop. In the workshop, you will learn how to perform differential expression analysis on raw counts using <strong><code>DESeq2</code></strong> <span class="citation" data-cites="DESeq2">(<a href="#ref-DESeq2" role="doc-biblioref">Love, Huber, and Anders 2014</a>)</span> or on logged normalised expression values using <strong><code>scran</code></strong> <span class="citation" data-cites="scran">(<a href="#ref-scran" role="doc-biblioref">Lun, McCarthy, and Marioni 2016</a>)</span> or both.</p>
</section>
<section id="omics-3-visualising-and-interpreting" class="level2">
<h2 class="anchored" data-anchor-id="omics-3-visualising-and-interpreting">Omics 3: Visualising and Interpreting</h2>
<p>before</p>
<ul>
<li>recap what we have</li>
<li>PCA</li>
<li>volcano plot described</li>
<li>GO terms</li>
<li></li>
</ul>
<p>workshop</p>
<ul>
<li>open project import the data</li>
<li>revise!</li>
<li>PCA</li>
<li>volcano plot</li>
<li>kable table? knitr::kable(df) |&gt; kableExtra::kable_styling()</li>
<li>annotating with go terms</li>
</ul>
<p>after</p>
<ul>
<li>document what you have done</li>
<li>repeat on another comparison</li>
</ul>
<p>References</p>



</section>
</section>

</main> <!-- /main -->
<div id="quarto-appendix" class="default"><section class="quarto-appendix-contents" role="doc-bibliography"><h2 class="anchored quarto-appendix-heading">References</h2><div id="refs" class="references csl-bib-body hanging-indent" role="list">
<div id="ref-DESeq2" class="csl-entry" role="listitem">
Love, Michael I., Wolfgang Huber, and Simon Anders. 2014. <span>“Moderated Estimation of Fold Change and Dispersion for RNA-Seq Data with DESeq2”</span> 15: 550. <a href="https://doi.org/10.1186/s13059-014-0550-8">https://doi.org/10.1186/s13059-014-0550-8</a>.
</div>
<div id="ref-scran" class="csl-entry" role="listitem">
Lun, Aaron T. L., Davis J. McCarthy, and John C. Marioni. 2016. <span>“A Step-by-Step Workflow for Low-Level Analysis of Single-Cell RNA-Seq Data with Bioconductor”</span> 5: 2122. <a href="https://doi.org/10.12688/f1000research.9501.2">https://doi.org/10.12688/f1000research.9501.2</a>.
</div>
</div></section></div></main> <!-- /main -->
<script id="quarto-html-after-body" type="application/javascript">
window.document.addEventListener("DOMContentLoaded", function (event) {
const toggleBodyColorMode = (bsSheetEl) => {
Expand Down
Loading

0 comments on commit ddbcd8b

Please sign in to comment.